Abnormal expression of TGFβ1 in acute myeloid leukemia and its regulation effect on leukemia cells

Objective:To explore the level of acute myeloid leukemia(COX-2),transforming growth factorβ1(TGFβ1),basic fibroblast growth factor(bFGF),and vascular endothelial growth factor(VEGF)in acute myeloid leukemia(AML)patients and to investigate the role of TGFβ1 on the proliferation,apoptosis and cycle of AML cells,providing new targets and research bases for the treatment and prognostic evaluation of AML.Methods:Peripheral blood was collected from 80 AML patients and 60 normal people.The levels of COX-2,TGFβ1,bFGF and VEGF in peripheral blood mononuclear cells and serum were determined by using quantitative polymerase chain reaction and enzyme linked immunosorbent assay,respectively.The proliferation,apoptosis and cycle of AML cells affected by overexpression and silencing of TGFβ1 was detected using cell counting kit-8 and flow cytometry.Results:The expression of COX-2,TGFβ1,bFGF and VEGF increased significantly in peripheral blood mononuclear cells and serum in AML patients.TGFβ1 promoted AML cell proliferation,inhibited its apoptosis,and increased stage G2 cell proportion.Conclusion:COX-2,TGFβ1,bFGF and VEGF play important roles in the progression of AML.TGFβ1 is a new potential biomarker for the diagnosis and treatment of AML.

Effect of Trastuzumab on the Micellization Properties, Endocytic Pathways and Antitumor Activities of Polyurethane-based Drug Delivery System

Polyurethane micelles（PM）-based nanovehicles have shown great potential in targeted delivery of therapeutics and diagnostics into tumors.However,the pathways of PMs entering cancer cells and the action mechanism of targeting ligands have yet to be understood.In this contribution,the actively-targeted PM were developed using trastuzumab as a model targeting group.It was found that PM were mainly taken up by SKOV-3 tumor cells via a micropinocytosis process,while the incorporation of trastuzumab to PM enabled a receptor-mediated endocytosis of nanocarriers in cancer cells,leading to more efficient cell entry and enhanced anticancer efficacy of chemotherapeutic drugs both in vitro and in vivo.This study is advantageous to the understanding of the action mechanism of trastuzumab,and significant for the construction of improved formulations for targeted delivery and precise therapy.