A new cyclopeptide from Dianthus caryophyllus

A new cyclopeptide as well as a known cyclopeptide were isolated from the whole plants of Dianthus caryophyllus. Their structures were elucidated as cyclo （-Thr-Gly-Pro-Tyr-Phe-） （1） and cyclo （-Ala-Gly-Ser-IIe-Phe-Phe-） （2） by means of chemical and spectroscopic methods including MS, 1D and 2D NMR techniques.

生长调节剂对香石竹(Dianthus caryophyllus)组培苗生根的影响

为了解不同生长调节剂及其浓度对香石竹组培苗生根培养的影响,通过在基本培养基内添加不同浓度的IAA和IBA进行组织培养试验并观察其生根和生长情况,来探索促进香石竹组培苗生根的最佳生长调节剂种类及其浓度。研究结果表明,10%次氯酸钠灭菌4 min时灭菌效果最佳;MS培养基中植株的根长与根数明显高于1/2MS和2MS培养基,而且生根较快,生根率高,植株长势好;1.0 mg/L的IAA和IBA都有利于根的伸长,并显著增加根数,其余浓度促进效果不显著,且浓度过高会抑制根的生长。各浓度IBA处理的植株长势均较好,其中1.0 mg/L的IBA处理的植株长势最好,最有利于香石竹组培苗生根培养。上述研究结果为香石竹切花离体快繁及工厂化生产提供进一步的理论依据。

Molecular Cloning and Characterization of Carnation EBF1 Gene During Flower Senescence and upon Ethylene Exposure and Sugar

A cDNA clone encoding a putative EBF-like protein （DCEBF1）was obtained from total RNA isolated from senescing carnation （Dianthus caryophyllus L.） petals using reverse transcription PCR and rapid-amplification of cDNA ends techniques. The cDNA contained an open reading frame of l 878 bp corresponding to 625 amino acids. Results of Northern blot indicated DCEBFI expression was enhanced by endogenous and exogenous ethylene, and was inhibited by STS in petals and ovaries. Upon wounding treatment, DCEBF1 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The levels of DCEBF1 mRNA increased in both petals and ovaries by sucrose treatment compared with the control.

香石竹Dccdc20基因的克隆及表达分析

减数分裂是香石竹遗传改良的重要环节,Cdc20蛋白在减数分裂中起着重要作用。为了更好的了解Cdc20在香石竹减数分裂中的作用机制。本研究利用RT-qPCR结合RACE技术,从香石竹花药中分离克隆了一个Cdc20基因的全长c DNA序列,命名为Dccdc20 (GenBanK登录号:2217177)。Dccdc20基因全长1 847 bp,含有439个氨基酸,长1 320 bp的开放阅读框。蛋白序列比对显示Dccdc20基因与其他物种的Cdc20基因有很高的相似度,RT-qPCR结果显示Dccdc20基因的表达量随着花药发育呈现递减趋势,在花蕾长度为1.1~1.2 cm时期的花药中表达最高,而在叶和茎中表达量较低,研究表明该基因可能在调节香石竹减数分裂中发挥作用。

细胞分裂素处理对香石竹切花保鲜效果的影响

以香石竹切花为试材,通过测定其花径、瓶插寿命、鲜质量、水分平衡、可溶性蛋白质、相对电导率等指标,研究了6-苄氨基嘌呤(6-BA)、激动素(KT)、玉米素(ZT)3种细胞分裂素处理的瓶插液对香石竹切花保鲜效果的影响,以期筛选出理想的香石竹切花保鲜液。结果表明:在蒸馏水和可利鲜对照下,30 g·L-1 Suc(蔗糖)+250 mg·L-1 CA(柠檬酸)+200 mg·L-1 8-HQS(8-羟基喹啉硫酸盐)+10 mg·L-1 6-BA和30 g·L-1 Suc+250 mg·L-1 CA+200 mg·L-1 8-HQS+5 mg·L-1 ZT保鲜效果最佳。