Huanglongbing(HLB) is a devastating disease that has led to an acute crisis for growers of citrus, one of the world's most important fruit crops. The phloem-feeding Asian citrus psyllid(ACP), Diaphorina citri, is ...Huanglongbing(HLB) is a devastating disease that has led to an acute crisis for growers of citrus, one of the world's most important fruit crops. The phloem-feeding Asian citrus psyllid(ACP), Diaphorina citri, is the main pest at the new shoot stage and is the only natural vector of HLB pathogenic bacteria. Little is known about how plants perceive and defend themselves from this destructive pest. Here, we characterized changes in the expression of various genes in citrus plants that were continuously infested by D. citri for different durations(12, 24, and 48 h). A total of 5 219 differentially expressed genes(DEGs) and 643 common DEGs were identified across all time points. Several pathways related to defense were activated, such as peroxisome, alpha-linolenic acid metabolism, and phenylpropanoid and terpenoid biosynthesis, and some pathways related to growth and signal transduction were suppressed in response to D. citri infestation. The expression of genes including kinases(CML44, CIPK6, and XTH6), phytohormones(SAMT, LOX6, and NPR3), transcription factors(bHLH162, WRKY70, and WRKY40), and secondary metabolite synthesis-related genes(PAL, 4CL2, UGT74B1 and CYP82G1) was significantly altered in response to D. citri infestation. The findings of this study greatly enhance our understanding of the mechanisms underlying the defense response of citrus plants to D. citri infestation at the molecular level. Functional characterization of the candidate defense-related genes identified in this study will aid the molecular breeding of insect-resistant citrus varieties.展开更多
In order to promote the healthy and sustainable development of citrus industry in Guangxi and reduce the harm of Diaphorina citri and citrus Huanglongbing on citrus industry,the paper summarizes the occurrence of D.ci...In order to promote the healthy and sustainable development of citrus industry in Guangxi and reduce the harm of Diaphorina citri and citrus Huanglongbing on citrus industry,the paper summarizes the occurrence of D.citri in Guangxi,and put forward comprehensive control technique,so as to provide the reference for guiding prevention and control of major pests in citrus industry.展开更多
Nicotine and nornicotine-typed compounds are well-known for its potential as insecticide.Some researches have shown that nicotine was highly toxic to Diaphorina citri Kuwayama,an invasive and fast spreading pest.Here ...Nicotine and nornicotine-typed compounds are well-known for its potential as insecticide.Some researches have shown that nicotine was highly toxic to Diaphorina citri Kuwayama,an invasive and fast spreading pest.Here we report the effects of nornicotine-typed insecticide against D.citri.Novel compounds A1-A8,B1-B28,C1-C18 and D1-D12 were synthesized by reported procedure.And bioassays indicated that some of these compounds exhibited good effects against D.citri.Compounds B2,B3,B8,B12,B13,B22,C5,C7,D5,and D9 exhibited the mortality up to 100%within 120 h in 50 mg/L.And further data shows that A2-5,A7,B1,B8,B20 and B22 showed rapid insecticidal activities against D.citri in 24 h with the low LC_(50)(LC_(50)<50 mg/L in 24 h,and LC_(50)<30 mg/L in 72 h).Nornicotine-typed derivatives exhibited activity against D.citri,and B8 and B22 exhibited the best insecticidal activity with an LC_(50)of 18.01.0,18.20.9 mg/L in 24 h,which had the lower cost than nicotine and showed the potential as powerful insecticides.展开更多
基金supported by Key Realm R&D Program of Guangdong Province (Grant No. 2020B0202090005)Special Fund for Scientific Innovation Strategy-construction of High Level Academy of Agriculture Science (Grant No. R2020PY-JG002)the President Foundation of Guangdong Academy of Agricultural Sciences (Grant No. 202030)。
文摘Huanglongbing(HLB) is a devastating disease that has led to an acute crisis for growers of citrus, one of the world's most important fruit crops. The phloem-feeding Asian citrus psyllid(ACP), Diaphorina citri, is the main pest at the new shoot stage and is the only natural vector of HLB pathogenic bacteria. Little is known about how plants perceive and defend themselves from this destructive pest. Here, we characterized changes in the expression of various genes in citrus plants that were continuously infested by D. citri for different durations(12, 24, and 48 h). A total of 5 219 differentially expressed genes(DEGs) and 643 common DEGs were identified across all time points. Several pathways related to defense were activated, such as peroxisome, alpha-linolenic acid metabolism, and phenylpropanoid and terpenoid biosynthesis, and some pathways related to growth and signal transduction were suppressed in response to D. citri infestation. The expression of genes including kinases(CML44, CIPK6, and XTH6), phytohormones(SAMT, LOX6, and NPR3), transcription factors(bHLH162, WRKY70, and WRKY40), and secondary metabolite synthesis-related genes(PAL, 4CL2, UGT74B1 and CYP82G1) was significantly altered in response to D. citri infestation. The findings of this study greatly enhance our understanding of the mechanisms underlying the defense response of citrus plants to D. citri infestation at the molecular level. Functional characterization of the candidate defense-related genes identified in this study will aid the molecular breeding of insect-resistant citrus varieties.
基金Supported by Science and Technology Major Project of Guangxi Province(GK AA20108003,GK AA22036002)Science and Technology Major Project of Nanning City(20212141)+6 种基金Collaborative Innovation Project of Chinese Academy of Agricultural Sciences-Guangxi Academy of Agricultural Sciences(CAAS-GXAAS-XTCX2019026-2)Guangxi Special Crops Experimental Station(G TS202201)Wuming District Science and Technology Project of Nanning City(2210201)Jiangnan District Science and Technology Project of Nanning City(2020020102)GNK(2021YT051,2022JM32)Guangxi Citrus Huanglongbing Prevention and Control Engineering Technology Research Center"Cultivation and Pest Control"Post Expert of Guangxi Citrus Innovation Team(nycytxgxcxtd-2021-05-02)。
文摘In order to promote the healthy and sustainable development of citrus industry in Guangxi and reduce the harm of Diaphorina citri and citrus Huanglongbing on citrus industry,the paper summarizes the occurrence of D.citri in Guangxi,and put forward comprehensive control technique,so as to provide the reference for guiding prevention and control of major pests in citrus industry.
基金the financial support from the National Natural Science Foundation of China(31901786,22078263)the National Key R&D Program of China(2021YFC2101500)+2 种基金the National Key R&D Program of China(2021SF-116,2023-YBSF-396)the Natural Science Foundation of Shaanxi Province(2022JQ-112)the China Postdoctoral Science Foundation(2021M692610).
文摘Nicotine and nornicotine-typed compounds are well-known for its potential as insecticide.Some researches have shown that nicotine was highly toxic to Diaphorina citri Kuwayama,an invasive and fast spreading pest.Here we report the effects of nornicotine-typed insecticide against D.citri.Novel compounds A1-A8,B1-B28,C1-C18 and D1-D12 were synthesized by reported procedure.And bioassays indicated that some of these compounds exhibited good effects against D.citri.Compounds B2,B3,B8,B12,B13,B22,C5,C7,D5,and D9 exhibited the mortality up to 100%within 120 h in 50 mg/L.And further data shows that A2-5,A7,B1,B8,B20 and B22 showed rapid insecticidal activities against D.citri in 24 h with the low LC_(50)(LC_(50)<50 mg/L in 24 h,and LC_(50)<30 mg/L in 72 h).Nornicotine-typed derivatives exhibited activity against D.citri,and B8 and B22 exhibited the best insecticidal activity with an LC_(50)of 18.01.0,18.20.9 mg/L in 24 h,which had the lower cost than nicotine and showed the potential as powerful insecticides.
