Objectives: To identify the etiology and source after recognition of diarrhea outbreak is associated with drinking water in suburb of Chengdu. Methods: Both unmatched case control and retrospective cohort study were c...Objectives: To identify the etiology and source after recognition of diarrhea outbreak is associated with drinking water in suburb of Chengdu. Methods: Both unmatched case control and retrospective cohort study were conducted. 131 targets including 56 suspected patients were recruited for case control study, while 463 residents were selected for cohort study. Stool, water and environmental samples were collected for laboratory testing. Results: The proportion of case exposed to well water was 86% in case group compared with 51% in the controls during the epidemic period (OR = 6.14, P P P E. coli and Enterotoxigenic E. coli was positive in stool and river samples. Conclusions: This outbreak of diarrhea might be caused by several mixed opportunistic pathogens in well water contaminated from the river water.展开更多
Vibrio cholera, causing acute watery diarrhea known as cholera disease, affects all ages and both genders. Cholera infection outbreaks in Iraq have been reported for several years. The recent cholera outbreak, emerged...Vibrio cholera, causing acute watery diarrhea known as cholera disease, affects all ages and both genders. Cholera infection outbreaks in Iraq have been reported for several years. The recent cholera outbreak, emerged throughout 2015, was investigated using bacteriological laboratory tests, singleplex and multiplex PCR technique for the detection of V. cholera from stool samples. Furthermore the toxigenic potential coupled with the antibiotic susceptibility test for cholera and other bacteria were also investigated. The stool samples were collected from 5698 patients admitted to Al-Yarmouk Teaching hospital and health care centers in Baghdad/Al-Karkh, Iraq, from the 1<sup>st</sup> of August to the 30<sup>th</sup> of December 2015. The V. cholera was isolated from 194 cases (3.4% of the cases age between 21 - 50 years). In addition, other enteric infections: Salmonellosis and Shigellosis 7 and 21 respectively, protozoan parasite Giardia lamblia and Entamoeba histolytica 2 and 43 cases respectively were also reported. High percentage of V. cholera infection was detected in October (122 cases, 62.8%), compared with other enteric infections that show high percentage of diarrheal disease in September and November. The results have confirmed that the cholera outbreak was caused by V. cholera O1, biotype El Tor, and serotype Inaba. Seven virulence genes were identified ctxA, toxR, zot, ace, rfbO1, tcpA and ompW. Moreover, the cholera isolated strains were found sensitive to most antibiotic but resistant to nalidixic acid.展开更多
目的分析2015年4月河北某小区感染性腹泻暴发疫情的病原学特征。方法采集该次暴发疫情的19份便和1份呕吐物标本并采用特异探针实时荧光定量RT-PCR(Taq Man real time reverse transcription PCR)技术进行检测,对阳性标本进行基因测序和...目的分析2015年4月河北某小区感染性腹泻暴发疫情的病原学特征。方法采集该次暴发疫情的19份便和1份呕吐物标本并采用特异探针实时荧光定量RT-PCR(Taq Man real time reverse transcription PCR)技术进行检测,对阳性标本进行基因测序和型别鉴定,进化树分析。结果采集的20份标本检出诺如病毒核酸阳性15份,阳性检出率为75%,均为诺如病毒Ⅱ型。对其中10株诺如病毒进行同源性及进化树分析显示均归类于GⅡ.17亚型。结论本次感染性腹泻暴发疫情由诺如病毒GⅡ.17亚型所致。展开更多
文摘Objectives: To identify the etiology and source after recognition of diarrhea outbreak is associated with drinking water in suburb of Chengdu. Methods: Both unmatched case control and retrospective cohort study were conducted. 131 targets including 56 suspected patients were recruited for case control study, while 463 residents were selected for cohort study. Stool, water and environmental samples were collected for laboratory testing. Results: The proportion of case exposed to well water was 86% in case group compared with 51% in the controls during the epidemic period (OR = 6.14, P P P E. coli and Enterotoxigenic E. coli was positive in stool and river samples. Conclusions: This outbreak of diarrhea might be caused by several mixed opportunistic pathogens in well water contaminated from the river water.
文摘Vibrio cholera, causing acute watery diarrhea known as cholera disease, affects all ages and both genders. Cholera infection outbreaks in Iraq have been reported for several years. The recent cholera outbreak, emerged throughout 2015, was investigated using bacteriological laboratory tests, singleplex and multiplex PCR technique for the detection of V. cholera from stool samples. Furthermore the toxigenic potential coupled with the antibiotic susceptibility test for cholera and other bacteria were also investigated. The stool samples were collected from 5698 patients admitted to Al-Yarmouk Teaching hospital and health care centers in Baghdad/Al-Karkh, Iraq, from the 1<sup>st</sup> of August to the 30<sup>th</sup> of December 2015. The V. cholera was isolated from 194 cases (3.4% of the cases age between 21 - 50 years). In addition, other enteric infections: Salmonellosis and Shigellosis 7 and 21 respectively, protozoan parasite Giardia lamblia and Entamoeba histolytica 2 and 43 cases respectively were also reported. High percentage of V. cholera infection was detected in October (122 cases, 62.8%), compared with other enteric infections that show high percentage of diarrheal disease in September and November. The results have confirmed that the cholera outbreak was caused by V. cholera O1, biotype El Tor, and serotype Inaba. Seven virulence genes were identified ctxA, toxR, zot, ace, rfbO1, tcpA and ompW. Moreover, the cholera isolated strains were found sensitive to most antibiotic but resistant to nalidixic acid.
文摘目的分析2015年4月河北某小区感染性腹泻暴发疫情的病原学特征。方法采集该次暴发疫情的19份便和1份呕吐物标本并采用特异探针实时荧光定量RT-PCR(Taq Man real time reverse transcription PCR)技术进行检测,对阳性标本进行基因测序和型别鉴定,进化树分析。结果采集的20份标本检出诺如病毒核酸阳性15份,阳性检出率为75%,均为诺如病毒Ⅱ型。对其中10株诺如病毒进行同源性及进化树分析显示均归类于GⅡ.17亚型。结论本次感染性腹泻暴发疫情由诺如病毒GⅡ.17亚型所致。