The median lethal concentrations (LC50s) of aldrin, fenvalerate, captan and diazinon were determined for Clarias batrachus by trimmed Spearman-Karber method. The potency ratios of toxicity among them were analysed by ...The median lethal concentrations (LC50s) of aldrin, fenvalerate, captan and diazinon were determined for Clarias batrachus by trimmed Spearman-Karber method. The potency ratios of toxicity among them were analysed by parallel-line bioassay with quantal responses. The LC50s for 40 day of exposure of aldrin, fenvalerate, captan and diazinon were 0.00036,0.0094, 0.5473 and 2.4186 ppm respectively. These values were lower than those obtained for an exposure of 96 hour. It shows the greater toxicity of the pesticides in a long-term exposure. The relative toxic potency of aldrin fenvalerate, captan and diazinon was in a ratio of 6807:241:4:1 respectively. Thus the chemically different groups of pesticides exhibit an order of toxicity as aldrin > fenvalerate >captan > diazinon for the freshwater catfish, Clarias batrachus. It infers that the catfish is most sensitive to aldrin and least sensitive to diazinon. The comparison of the sensitivity of various species tested against these pesticidal chemicals has also been done to review the available information.展开更多
In most tropical developing countries, one of the problems facing aquaculture industry is the pollution of ponds and rivers with pesticides. Chemicals such as diazinon, an organophosphate pesticide, originating from a...In most tropical developing countries, one of the problems facing aquaculture industry is the pollution of ponds and rivers with pesticides. Chemicals such as diazinon, an organophosphate pesticide, originating from agricultural activity enter the aquatic environment through atmospheric deposition, surface run-off or leaching. Pollutants enter the food chain through accumulation in soft bottom sediment and aquatic organisms. However, information on how these pesticides affect inhabiting organisms is often not available. In a triplicate experimental set-up, seventy-two (72) apparently healthy catfish comprising adult and juvenile of both sexes were therefore exposed to a previously determined no effect concentration (0.405 ppm) of diazinon. Another set of fish was exposed to 0.0625 μg sodium arsenite, a known clastogen, which was used as the positive control, while another set of catfish exposed to the culture water alone was the negative control. Adults and juveniles were exposed separately to avoid cannibalism. After 48 hours of exposure, micronuclei induction was determined in subsets of experimental groups, while exposure continued for 28 days. Catfish organs were harvested on days 21 and 28 to determine the effect of long-term exposure to diazinon on histology. Water quality was also monitored before and during exposure in the experimental groups. The result established a significantly high mean micronucleated polychromatic erythrocytes (15.00) in catfish exposed to diazinon suggesting genetic damage (normal is ≤4). The MPE in sodium arsenite exposed fish was 28, while that of the control group was below 4. Effect of sex and age on micronuclei induction was not significant. Histological alteration observed in the ovary and testis was distorted matured cells and extensive testicular degeneration, respectively. The results show that diazinon has clastogenic effect, and may have endocrine disrupting properties because of the histological changes induced in the ovaries and testis.展开更多
A neurophysiological detector (NPD) is a hybridization of olfactory system neurons of the fish crucian carp, Carassius carassius L., with a computerized electronic device connected to a high-performance liquid chromat...A neurophysiological detector (NPD) is a hybridization of olfactory system neurons of the fish crucian carp, Carassius carassius L., with a computerized electronic device connected to a high-performance liquid chromatograph (HPLC). This system makes it possible to measure neurophysiological activities in the olfactory system of C. carassius L. after exposure of this fish to alarm pheromones. The construction of the system was presented for the first time at the 3rd International Symposium on Separation in Bio Sciences SBS 2003 in I. Brondz, et al., The Fish Olfactory System Used as an In-Line HPLC Neurophysiologic Detector NPD, 3rd Int. Symposium on Separation in Bio Sciences SBS 2003: A 100 Years of Chromatography, 13-18 May, Moscow, Russia, 2003, Abstract O- 27, p. 95. A complete paper was published in I. Brondz, et al., Neurophysiologic Detector (NPD)—A Selective and Sensitive Tool in High-Performance Liquid Chromatography, Chromatography B: Biomedical Sciences and Applications, Vol. 800, No. 1-2, 2004, pp. 41-47, and the hybridization of living cells with an electronic device has been discussed (I. Brondz, et al., International Scientific-Technical Conference Sensors Electronics and Microsystems Technology (SEMST-1), 1-5 June, (Odessa), Ukraine, 2004, Plenum Lecture, Abstract p. 17;I. Brondz, et al., The European Chemoreception Research Organization ECRO 2004 Congress, 12-15 September, (Dijon), France, 2004, Abstract P-3;and I. Brondz, et al., Biosensors as Electronic Compounds for Detector in the High-Performance Liquid Chromatography (HPLC), Electronic Components and Systems, Vol. 3, No. 103, 2006, pp. 25-27). In the present study, an HPLC equipped with an NPD was used to assess the influence of organophosphate (OP) pesticides on olfactory sensory nerves and the modification of nerve signals from the olfactory organ. The results show that exposure of the olfactory system to OP pesticides can lead to disruption of normal reflexes and to significant suppression of individual sexual activity and, as a result, to the suppression of a population.展开更多
The toxicokinetics, tissue distribution, and anticholinesteruse (antiChE ) activity of diazinon were investigated in the rat. Plasma concentrations most adequately fitted a two-compartment open model after iv adminis...The toxicokinetics, tissue distribution, and anticholinesteruse (antiChE ) activity of diazinon were investigated in the rat. Plasma concentrations most adequately fitted a two-compartment open model after iv administration of 10 mg/kg and a one-compartment model after oral administration of 80 mg/kg. Diazinon elimination half-life following iv and oral dosing was 4.70 and 2.86 h, respectively. The oral bioavailabllity was found to be low (35.5%). Hepatic extraction ratios after iv administration of 5 or 10 mg展开更多
The study was carried out with the objective of developing suitable and sustainable low cost adsorbent materials for diazinon, an organophosphate pollutant used as a pesticide. Montmorillonite modified with iron was u...The study was carried out with the objective of developing suitable and sustainable low cost adsorbent materials for diazinon, an organophosphate pollutant used as a pesticide. Montmorillonite modified with iron was used. Two different types of iron-montmorillonite, each having different contents of iron and synthesized with different pH and levels of Fe hydrolysis were used. One was denoted “Fe-modified” and the other denoted as “FeOH-modified”. The color of the samples changed from greyish green to light-reddish brown after the modification. X-ray diffraction and physical observations were used for characterization of the samples. The d-spacing of the samples was greater than 15 ?, indicating the formation of iron hydroxides in the interlayer space of montmorillonite. The amount of adsorption was calculated from the difference between the initial and the final concentration of diazinon. The adsorption data were analyzed using the Langmuir adsorption isotherms. The amounts of diazinon adsorbed were 58.8 and 54.1 mmolkg-1 for Fe-modified and FeOH-modified respectively. The steep rise in their adsorption isotherms indicated the possibility of adsorption for low level of diazinon in polluted water.展开更多
In all parts of the world pesticides have been found in the aquatic ecosystem and scientific evidence has also shown that they can enter the food chain. Diazinon is an organophosphate pesticide, widely used in agricul...In all parts of the world pesticides have been found in the aquatic ecosystem and scientific evidence has also shown that they can enter the food chain. Diazinon is an organophosphate pesticide, widely used in agriculture to control a wide variety of suckling and leaf eating insects and recently in fish culture to suppress some parasitic diseases;nevertheless, there is little study on its adverse effect on fish. In this study, seventy-two (72) apparently healthy catfish comprising adult and juvenile of both sexes were used to set up triplicate experimental groups of those exposed to culture water alone (control group), fish exposed to pre-determined no-effect concentration (0.405 ppm) of Diazinon (test group). The fish were exposed for 28 days and fish were sacrificed and organs harvested on days 21 and 28 to determine the effect of long-term exposure to diazinon on organ histology. Histological changes observed in diazinon-exposed catfish were hyperplasia and fusion of the gill epithelium, hyperplasia of mucoid producing cells and aggregation of melanin pigment in the skin. Histological lesions were also seen observed in other organs, including severe diffuse cellular swelling and fatty degeneration of the liver, interstitial congestion of the kidney, carbon deposit on the wall of the heart and multifocal haemorrhage. The water quality of the control was not significantly different from that of the test group throughout the experiment. The lesions detected in cells, tissue, or organs represent an integration of cumulative effects of physiological and biochemical stressors. The histological alterations observed in vital organ of fish show that exposure to “no-effect” concentration of diazinon induced structural damage in fish organs and are likely to affect the functionality of the organs. For example, the adverse effect on the gill might disrupt its feeding and oxygen uptake.展开更多
A rapid method was developed for the determination of diazinon in plasma using gas chromatography with electron-capture detection (GC-ECD). Following a single extraction with hexane from 100μl of plasma, diazinon was...A rapid method was developed for the determination of diazinon in plasma using gas chromatography with electron-capture detection (GC-ECD). Following a single extraction with hexane from 100μl of plasma, diazinon was quantitated on a 3% OV-17 column. The detection limit was 10 ng/ml and linearity was obtained in the range of 25 ng/ml-2500 ng/ml.The applicability of the assay to single-dose kinetics in rats is illustrated展开更多
文摘The median lethal concentrations (LC50s) of aldrin, fenvalerate, captan and diazinon were determined for Clarias batrachus by trimmed Spearman-Karber method. The potency ratios of toxicity among them were analysed by parallel-line bioassay with quantal responses. The LC50s for 40 day of exposure of aldrin, fenvalerate, captan and diazinon were 0.00036,0.0094, 0.5473 and 2.4186 ppm respectively. These values were lower than those obtained for an exposure of 96 hour. It shows the greater toxicity of the pesticides in a long-term exposure. The relative toxic potency of aldrin fenvalerate, captan and diazinon was in a ratio of 6807:241:4:1 respectively. Thus the chemically different groups of pesticides exhibit an order of toxicity as aldrin > fenvalerate >captan > diazinon for the freshwater catfish, Clarias batrachus. It infers that the catfish is most sensitive to aldrin and least sensitive to diazinon. The comparison of the sensitivity of various species tested against these pesticidal chemicals has also been done to review the available information.
文摘In most tropical developing countries, one of the problems facing aquaculture industry is the pollution of ponds and rivers with pesticides. Chemicals such as diazinon, an organophosphate pesticide, originating from agricultural activity enter the aquatic environment through atmospheric deposition, surface run-off or leaching. Pollutants enter the food chain through accumulation in soft bottom sediment and aquatic organisms. However, information on how these pesticides affect inhabiting organisms is often not available. In a triplicate experimental set-up, seventy-two (72) apparently healthy catfish comprising adult and juvenile of both sexes were therefore exposed to a previously determined no effect concentration (0.405 ppm) of diazinon. Another set of fish was exposed to 0.0625 μg sodium arsenite, a known clastogen, which was used as the positive control, while another set of catfish exposed to the culture water alone was the negative control. Adults and juveniles were exposed separately to avoid cannibalism. After 48 hours of exposure, micronuclei induction was determined in subsets of experimental groups, while exposure continued for 28 days. Catfish organs were harvested on days 21 and 28 to determine the effect of long-term exposure to diazinon on histology. Water quality was also monitored before and during exposure in the experimental groups. The result established a significantly high mean micronucleated polychromatic erythrocytes (15.00) in catfish exposed to diazinon suggesting genetic damage (normal is ≤4). The MPE in sodium arsenite exposed fish was 28, while that of the control group was below 4. Effect of sex and age on micronuclei induction was not significant. Histological alteration observed in the ovary and testis was distorted matured cells and extensive testicular degeneration, respectively. The results show that diazinon has clastogenic effect, and may have endocrine disrupting properties because of the histological changes induced in the ovaries and testis.
文摘A neurophysiological detector (NPD) is a hybridization of olfactory system neurons of the fish crucian carp, Carassius carassius L., with a computerized electronic device connected to a high-performance liquid chromatograph (HPLC). This system makes it possible to measure neurophysiological activities in the olfactory system of C. carassius L. after exposure of this fish to alarm pheromones. The construction of the system was presented for the first time at the 3rd International Symposium on Separation in Bio Sciences SBS 2003 in I. Brondz, et al., The Fish Olfactory System Used as an In-Line HPLC Neurophysiologic Detector NPD, 3rd Int. Symposium on Separation in Bio Sciences SBS 2003: A 100 Years of Chromatography, 13-18 May, Moscow, Russia, 2003, Abstract O- 27, p. 95. A complete paper was published in I. Brondz, et al., Neurophysiologic Detector (NPD)—A Selective and Sensitive Tool in High-Performance Liquid Chromatography, Chromatography B: Biomedical Sciences and Applications, Vol. 800, No. 1-2, 2004, pp. 41-47, and the hybridization of living cells with an electronic device has been discussed (I. Brondz, et al., International Scientific-Technical Conference Sensors Electronics and Microsystems Technology (SEMST-1), 1-5 June, (Odessa), Ukraine, 2004, Plenum Lecture, Abstract p. 17;I. Brondz, et al., The European Chemoreception Research Organization ECRO 2004 Congress, 12-15 September, (Dijon), France, 2004, Abstract P-3;and I. Brondz, et al., Biosensors as Electronic Compounds for Detector in the High-Performance Liquid Chromatography (HPLC), Electronic Components and Systems, Vol. 3, No. 103, 2006, pp. 25-27). In the present study, an HPLC equipped with an NPD was used to assess the influence of organophosphate (OP) pesticides on olfactory sensory nerves and the modification of nerve signals from the olfactory organ. The results show that exposure of the olfactory system to OP pesticides can lead to disruption of normal reflexes and to significant suppression of individual sexual activity and, as a result, to the suppression of a population.
文摘The toxicokinetics, tissue distribution, and anticholinesteruse (antiChE ) activity of diazinon were investigated in the rat. Plasma concentrations most adequately fitted a two-compartment open model after iv administration of 10 mg/kg and a one-compartment model after oral administration of 80 mg/kg. Diazinon elimination half-life following iv and oral dosing was 4.70 and 2.86 h, respectively. The oral bioavailabllity was found to be low (35.5%). Hepatic extraction ratios after iv administration of 5 or 10 mg
文摘The study was carried out with the objective of developing suitable and sustainable low cost adsorbent materials for diazinon, an organophosphate pollutant used as a pesticide. Montmorillonite modified with iron was used. Two different types of iron-montmorillonite, each having different contents of iron and synthesized with different pH and levels of Fe hydrolysis were used. One was denoted “Fe-modified” and the other denoted as “FeOH-modified”. The color of the samples changed from greyish green to light-reddish brown after the modification. X-ray diffraction and physical observations were used for characterization of the samples. The d-spacing of the samples was greater than 15 ?, indicating the formation of iron hydroxides in the interlayer space of montmorillonite. The amount of adsorption was calculated from the difference between the initial and the final concentration of diazinon. The adsorption data were analyzed using the Langmuir adsorption isotherms. The amounts of diazinon adsorbed were 58.8 and 54.1 mmolkg-1 for Fe-modified and FeOH-modified respectively. The steep rise in their adsorption isotherms indicated the possibility of adsorption for low level of diazinon in polluted water.
文摘In all parts of the world pesticides have been found in the aquatic ecosystem and scientific evidence has also shown that they can enter the food chain. Diazinon is an organophosphate pesticide, widely used in agriculture to control a wide variety of suckling and leaf eating insects and recently in fish culture to suppress some parasitic diseases;nevertheless, there is little study on its adverse effect on fish. In this study, seventy-two (72) apparently healthy catfish comprising adult and juvenile of both sexes were used to set up triplicate experimental groups of those exposed to culture water alone (control group), fish exposed to pre-determined no-effect concentration (0.405 ppm) of Diazinon (test group). The fish were exposed for 28 days and fish were sacrificed and organs harvested on days 21 and 28 to determine the effect of long-term exposure to diazinon on organ histology. Histological changes observed in diazinon-exposed catfish were hyperplasia and fusion of the gill epithelium, hyperplasia of mucoid producing cells and aggregation of melanin pigment in the skin. Histological lesions were also seen observed in other organs, including severe diffuse cellular swelling and fatty degeneration of the liver, interstitial congestion of the kidney, carbon deposit on the wall of the heart and multifocal haemorrhage. The water quality of the control was not significantly different from that of the test group throughout the experiment. The lesions detected in cells, tissue, or organs represent an integration of cumulative effects of physiological and biochemical stressors. The histological alterations observed in vital organ of fish show that exposure to “no-effect” concentration of diazinon induced structural damage in fish organs and are likely to affect the functionality of the organs. For example, the adverse effect on the gill might disrupt its feeding and oxygen uptake.
文摘A rapid method was developed for the determination of diazinon in plasma using gas chromatography with electron-capture detection (GC-ECD). Following a single extraction with hexane from 100μl of plasma, diazinon was quantitated on a 3% OV-17 column. The detection limit was 10 ng/ml and linearity was obtained in the range of 25 ng/ml-2500 ng/ml.The applicability of the assay to single-dose kinetics in rats is illustrated
