Psychological and Emotional Responses during Different Stages of the COVID-19 Pandemic Based on a Survey of a Mental Health Hotline

Background:The coronavirus(COVID-19)outbreak in 2019 triggered psychological and emotional responses.This research investigates the psychological status and emotional problems of those who sought psychological assistance during the epidemic period by calling a mental health hotline.Methods:This study aims to combine qualitative and quantitative research.Descriptive analysis was used for undertaking qualitative research.We ana-lyzed the data from group 1(n=706),in which the people used the mental health hotline from 25 January 2020 to 23 June 2020.A self-designed questionnaire was developed in accordance with the classification and sum-marized items from group 1’s psychological problems and emotional status.To implement the quantitative research,we conducted a cross-sectional descriptive survey and used the self-compiled scale and HADS to inves-tigate group 2(n=553)from May 2020 to June 2020.Results:Descriptive statistics and comparative analysis revealed that:①Visitors mainly reported behavior,emotional,family relationship problems and sleep disorders.②Anxiety,comorbidities,sleep disorders and coping problems were the most frequently reported problems.③There were significant differences in the number of visitors experiencing various problems or exhibiting harmful behaviors(sorrow,worry,fear,depression,sleep disorders,self-harm or suicide,and coping problems,anxiety,hypochondria,and comorbidity)in the four stages of the epidemic.④More than a quarter of participants still suffered from anxiety or depression in the later stages of the epidemic.Conclusion:Different problems manifested at different stages of the epidemic,and psychological interventions and assistance should be tailored to reflect this.

The Characterization of Aortic Valve Calcification at Different Stage of Disease

Cardiac valve calcification is a common disease,especially among the elderly.Calcification can affect valve function and cause heart failure and sudden death(Adler et al.,2002).Aortic valve calcification is alsorelated to arteriosclerosis and coronary heart disease(Rashedi et al.,2015).However,the origin of valve calcification is still unclear.This study characterized the

Microstructure and Stability of Fe_(73.5) Cu_1Nb_3Si_(13.5)B_9 Alloy at Different Stages of Phase Transformation

The microstructure and the stability of Fe_73.5Cu_1Nb_3Si_13.5B_9 alloy at different stages of phase transformation were investigated through the observation of X-ray difraction and transmission electron microscopy and the measurement of magnetic aftereffect (MAE). It was found that the dependence of the volume fraction of amorphous phase and the MAE in the samples annealed from 450 to 700℃ on the annealing temperature is similar.

Effect of intervention treatment from different stages on prognosis in children with central coordination disturbance

BACKGROUND： Central coordination disturbance is regarded as the early-stage symptom of brain injury-induced cerebral palsy. This disease manifests itself as motor disorder, abnormal attitudinal reflex and muscular tension. Early intervention may improve its prognosis. OBJECTIVE： To observe the effects of intervention treatment from different stages on the prognosis of central coordination disturbance following brain injury in children patients. DESIGN： A contrast observation experiment SETTING： Department of Neurology and Rehabilitation, Urumqi Children＇s Hospital PARTICIPANTS ： Totally 151 children who were diagnosed as central coordination disturbance from January 2002 to December 2003 in the Department of Neurology and Rehabilitation of Urumqi Children＇s Hospital were recruited. All the children patients, including 101 male and 50 female, aged from 3 months to 1 year old, met the diagnosis criteria of central coordination disturbance from Vojta. They were divided into slight, moderate and severe abnormity according to Vojta attitudinal reflex. All 151 patients were divided into 2 groups according to their age at diagnosis： 3-6 months old group （n=74）, 62 severe, 10 moderate and 2 slight; 7-12 months old group （n=77）, 66 severe, 7 moderate and 4 slight. All the relatives of children patients were informed of the experiment. METHODS： ① Both groups received physical training （PT） as the main treatment; Hand training was given if necessary. All of the patients received additional hyperbaric oxygen therapy, bioelectric therapy, scalp acupuncture, drug treatment and family training. The importance of integration of hospital and family based rehabilitation was stressed. Those who did not catch up with the normal development or had abnormal reflexes continued to receive treatment. ② Around the age of 2 years old, all children did a final evaluation using Bayley Scales of Infant Development （BSID）. Mental development index （MDI） and physical development index （PDI） were taken as evaluative criteria. Children with scores 80 and above on MDI and PDI were considered to have normal motor and mental development; 79 and below were considered delayers. The Diagnostic Criteria of Cerebral Palsy and Infantecondary School Social Adaptation Scale were used in the final evaluation[scores ≤5 was extremely severe （extremely severe cerebral palsy）, 6 was severe（severe cerebral palsy）, 7 was moderate（moderate cerebral palsy）, 8 was slight（slight cerebral palsy）, 9 was borderline（slight cerebral palsy）, 10 was normal, 11 was above average, 12 was excellent and ≥13 was extremely excellent]. ③ Final evaluative results of 2 groups were compared. And u test was used for the comparison of Bayley development index and Chi-square test for the comparison of normalization rate. MAIN OUTCOME MEASURES： BSID MDI and PDI as well as the normalization rate of motor function and mental behavior of children patients at 2 years old. RESULTS： Totally 151 children patients entered the stage of result analysis. ①Two groups of children patients were given integrated rehabilitation therapy , and evaluated at 2 years old . The 3-6 months old group had higher MDI and PDI than the 7-12 months old group （91.81＋19.99 vs 71.93＋18.98;91.55＋23.61 vs 68.95＋23.51, u=6.265,5.894,P 〈 0.01 ）.②After being given integrated rehabilitation therapy, children patients of 3-6 months old group had significantly higher normalization rate of motor function and mental behavior than 7-12 months old group （73% ,27%,X2=29.723 9,P 〈 0.01 ）. ③Six slight and 17 moderate central coordination disturbance children of 2 groups all completely recovered; After intervention treatment, among 128 severe central coordination disturbance children, 52 recovered and 76 were still abnormal, in which, 43 slight cerebral palsy, 18 moderate cerebral palsy, 5 severe cerebral palsy and 10 extremely severe cerebral palsy. CONCLUSION： To perform integrated intervention treatment before 6 months old can better improve and promote mental and physical development levels of children patients with central coordination disturbance after brain injury than 6 months later.

Combining urine surface-enhanced Raman spectroscopy with PCA-SVM algorithm for improving the identification of colorectal cancer at different stages

Cancer staging detection is important for clinician to assess the patients' status and make optimal therapy decision. In this study, the machine learning algorithm based on principal component analysis(PCA) and support vector machine(SVM) was combined with urine surface-enhanced Raman scattering(SERS) spectroscopy for improving the identification of colorectal cancer(CRC) at early and advanced stages. Two discriminant methods, linear discriminant analysis(LDA) and SVM were compared, and the results indicated that the diagnostic accuracy of SVM(93.65%) was superior to that of LDA(80.95%). This exploratory study demonstrated the great promise of urine SERS spectra along with PCA-SVM for facilitating more accurate detection of CRC at different stages.

Characteristics of Soil Organic Carbon and Total Nitrogen in Rubber Plantations Soil at Different Age Stages in the Western Region of Hainan Island

[Objective]The aim was to study on the characteristics of soil organic carbon and nitrogen in rubber （Hevea brasiliensis Muell-Arg） plantations at different age stages in the western region of Hainan Island,so as to evaluate the ecological benefits of rubber plantations and provide basic data for studying the effect of tropical land utilization/cover change on the global carbon and nitrogen cycle. [Method]The situs was in Danzhou city,western region of Hainan Island,and the samples were four kinds of rubber plantations soil at different ages and one kind of control check （pepper,Piper nigrum L.） soil. In this research,four quadrats were set up in each sample,and the size of each was 20 cm×20 cm. Four specimens were gathered from four layers of 0-15,15-30,30-45,45-60,and the average of them was the last analysis result of each sample. Soil density was measured by cutting ring method,soil containing and hygroscopic water was detected by oven drying method,soil organic carbon （SOC） was measured by low temperature heated outside potassium dichromate oxidation-colorimetry method,and soil total nitrogen （STN） was detected by semimicro Kjeldahl method. [Result]SOC contents of different layers in rubber plantations soil at different age stages （including the CK pepper soil,the same as below） varied little,and the content of SOC in surface layer （0-15 cm） was higher,while the underlayer （45-60 cm） was lower than the average value; there was significant difference in SOC content among different kinds of soil,and the content was of 6.03-7.78 g/kg,tapping young trees （7 years） CK pepper mature age trees （30 years） prophase of young trees （2 years） tapping trees （16 years）; there was no significant difference in SOC storage among different kinds of soil,and the storage was of 61.33-74.29 t/hm2,mature age trees （30 years） tapping young trees （7 years） prophase of young trees （2 years） CK pepper tapping trees （16 years）; there was significant difference in STN content among rubber plantations soil at different age stages,the content was of 410.86-664.14 mg/kg2,CK pepper tapping young trees （7 years） prophase of young trees （2 years） mature age trees （30 years） tapping trees （16 years）,and STN content of tapping trees （16 years） soil was extremely lowest; there was significant difference in C/N ratio among different kinds of soil,the ratio was of 10.94-14.47,and the ratio of tapping trees （16 years） mature age trees （30 years） tapping young trees （7 years） CK pepper prophase of young trees （2 years）. [Conclusion]There wasn＇t unhealthy effect of rubber trees planted in tropical area on the content and storage of SOC,the content of STN and the ratio of C/N. there was no significant difference between rubber plantations and CK pepper soil,and the effects of rubber plantation on soil carbon-nitrogen was similar to that of other tropical crops （such as pepper）.

Effects of Short-term High Temperature Stress on the Photosynthesis of Potato in Different Growth Stages

[Objective] The aim was to study the effects of short-term high temperature stress on the photosynthesis of potato in different growth stages. [Method] Choosing powder potato named Longshu No.3 widely cultivated in Ningxia as test material,the changes of stomata conductance （Gs）,transpiration rate （Tr） and CO2 concentration difference between internal and external leaf chamber,net photosynthetic rate （Pn） and photosynthetic water use efficiency （WUE） in different growth stages under short-term high temperature were analyzed. [Result] During seedling stage,the hysteretic nature of net photosynthetic rate and CO2 concentration difference between internal and external leaf chamber of potato could be found under high temperature stress,while the change trends of stomata conductance and transpiration rate under high temperature stress were consistent to that at normal temperature,but stomata conductance and transpiration rate were higher than those at normal temperature,and CO2 concentration difference between internal and external leaf chamber affected net photosynthetic rate most obviously. During branching stage,the change trends of net photosynthetic rate,CO2 concentration difference between internal and external leaf chamber,stomata conductance and transpiration rate under high temperature stress and normal temperature were similar,but they changed abruptly and reached peak value at noon under high temperature stress,while there existed consistent variation of water use efficiency under high temperature stress and at normal temperature,and CO2 concentration difference between internal and external leaf chamber also affected net photosynthetic rate most greatly,next came transpiration rate. [Conclusion] High temperature stress affected the photosynthesis of potato in different growth stages,and it was more obvious during branching stage than seedling stage,while CO2 concentration difference between internal and external leaf chamber had the most important influence on net photosynthetic rate.

Dynamic changes of genomic methylation profiles at different growth stages in Chinese Tan sheep

Background:Tan sheep,an important local sheep breed in China,is famous for their fur quality.One-month-old Tan sheep have white,curly hair with beautiful flower spikes,commonly known as“nine bends”,which has high economic value.However,the“nine bends”characteristic gradually disappears with age;consequently,the economic value of the Tan sheep decreases.Age-related changes in DNA methylation have been reported and may be responsible for age-induced changes in gene expression.Until now,no genome-wide surveys have been conducted to identify potential DNA methylation sites involved in different sheep growth stages.In this study we investigated the dynamic changes of genome-wide DNA methylation profiles in Tan sheep using DNA from skin and deep whole-genome bisulfite sequencing,and compared the DNA methylation levels at three different growth stages:1,24,and 48 months old(mon1,mon24,and mon48,respectively).Results:In this study,11 skin samples from three growth stages(four for mon1,four for mon24,and three for mon48)were used for DNA methylation analysis and gene expression profiling.There were 52,288 and 236 differentially methylated genes(DMGs)identified between mon1 and mon24,mon1 and mon48,and mon24 and mon48,respectively.Of the differentially methylated regions,1.11%,7.61%,and 7.65% were in the promoter in mon1 vs.mon24,mon24 vs.mon48,and mon1 vs.mon48,respectively.DMGs were enriched in the MAPK and WNT signaling pathways,which are related to age growth and hair follicle morphogenesis processes.There were 51 DMGs associated with age growth and curly fleece formation.Four DMGs between mon1 and mon48(KRT71,CD44,ROR2 and ZDHHC13)were further validated by bisulfite sequencing.Conclusions:This study revealed dynamic changes in the genomic methylation profiles of mon1,mon24,and mon48 sheep,and the percentages of methylated cytosines were 3.38%,2.85% and 4.17%,respectively.Of the DMGs,KRT71 and CD44 were highly methylated in mon1,and ROR2 and ZDHHC13 were highly methylated in mon48.These findings provide foundational information that may be used to develop strategies for potentially retaining the lamb fur and thus improving the economic value of Tan sheep.

Resistance of Different Tomato Varieties against Tomato Powdery Mildew during Different Growth Stages

The pathogen of tomato powdery mildew （Oidium neolycopersici Kiss） was simultaneously inoculated into four varieties during seedling, flowering and fruiting stages under the same environmental conditions by inducing inoculation method, so as to study the resistance during different growth stages. Different varieties of plants and the plants during different growth stages were investigated after inoculation for 12 d, and disease epidemic curves were drawn according to survey data. The results showed that different varieties performed different resistance against powdery mildew and the same variety also showed different resistance during different growth stages. The susceptible extent of Lujia was the heaviest during seedling and flowering sages, while it showed the strongest resistance during fruiting stage; improved 96 -8 showed the highest resistance during seedling stage, but performed the lowest resistance during fruiting stage.

Deformation Mechanism of Bimodal Structured 2205 Duplex Stainless Steel in Two Yield Stages

A kind of micro/nanostructured 2205 duplex stainless steel(DSS)with uniform distribution of nanocrystals was prepared via aluminothermic reaction method.The analysis of stress-strain curve showed that the fracture strength and elongation of the specimen were 946 MPa and 24.7%,respectively.At present,the research on microstructure of bimodal 2205 DSS at room temperature(RT)mainly depended on scanning electron microscope(SEM)observation after loading experiments.The test result indicates that there are two different yield stages in stress-strain curve of specimen during tensile process.The microstructure of duplex bimodal structured stainless steel consists of two pairs of soft hard regions and phases.By studying deformation mechanism of bimodal structured stainless steel,the interaction between soft phase and hard phase are discussed.The principle of composition design and microstructure control of typical duplex stainless steel is obtained,which provides an important research basis for designing of advanced duplex stainless steel.

Clinical Efficacy of Dasatinib in the Treatment of Chronic Myeloid Leukemia (CML) Patients with Different Clinical Stages

Objective:To study the efficacy of dasatinib treatment in different clinical stages of patients with chronic myeloid leukemia(CML).Methods:A total of 80 patients with chronic myeloid leukemia(CML)were selected for experimental research.According to different clinical stages,they were divided into chronic phase,accelerated phase and blast phase,and all of them were treated with dasatinib.Results:The complete cytogenetic response remission rate,complete hematologic remission rate,and major molecular biological remission rate in the chronic phase were significantly higher.Besides,the overall survival time and relapse-free survival time in the chronic phase were significantly longer,and the mortality during the follow-up period in the chronic phase was also significantly higher.Furthermore,the incidence of hematological adverse reactions of gradesⅢtoⅣin the chronic phase was significantly lower compared with the corresponding data of patients in the accelerated phase and blast phase with P<0.05.Conclusion:Different clinical stages of CML patients have different curative effects of dasatinib,which can effectively treat patients in chronic stage.

Deformation Stages and Ar-Ar Age Data of the Wan-Zhe-Gan Tectonic Zone,Southeast China,and Their Tectonic Significance

The major tectonic zone that passes through the border regions of the Anhui, Zhejiang, and Jiangxi Provinces in southeast China has been commonly referred to as the Wan-Zhe-Gan fault zone. Geologically, this zone consists of several regional fault belts of various ages and orientations. We have categorized the faults into four age groups based on field investigations. The Neoproterozoic faults are northeast striking. They start from the northeast Jiangxi Province and extend northeastward to Fuchuan in Anhui Province, the same location of the northeast Jiangxi-Fuchuan ophiolite belt. The faults probably acted during the Neoproterozoic as a boundary fault zone of a plate or a block suture with melange along the faults. The nearly east-west- or east-northeast-striking faults are of Silurian ages （40Ar/39Ar age 429 Ma）. This group includes the Qimen-Shexian fault and the Jiangwang-Jiekou ductile shear belt. They represent a major tectonic boundary in the basement because the two sides of the fault have clear dissimilarities. The third group of faults is north-northeast striking, having formed since the early-middle Triassic with 40Ar/39Ar ages of 230-254 Ma. They form a fault belt starting from Yiyang in northern Jiangxi and connect with the Wucheng as well as the Ningguo-Jixi faults. This fault belt is a key fault-magmatic belt controlling the formation of Jurassic-Cretaceous red basins, ore distribution, magmatic activity, and mineralization. When it reactivated during the late Cretaceous, the belt behaved as a series of reverse faults from southeast to northwest and composed the fourth fault group. Therefore, classifying the Wan-Zhe-Gan fault zone into four fault groups will help in the analysis of the tectonic evolution of the eastern segment of the Jiangnan orogen since the Neoproterozoic era.

Combining QTL mapping and expression profile analysis to identify candidate genes of cold tolerance from Dongxiang common wild rice(Oryza rufipogon Griff.)

Rice（Oryza sativa L.）, a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. Cold tolerance is affected by multiple genetic factors in rice, and the complex genetic mechanisms associated with chilling stress tolerance remain unclear. Here, we detected seven quantitative trait loci（QTLs） for cold tolerance at booting stage and identified one cold tolerant line, SIL157, in an introgression line population derived from a cross between the indica variety Guichao 2, as the recipient, and Dongxiang common wild rice, as the donor. When compared with Guichao 2, SIL157 showed a stronger cold tolerance during different growth stages. Through an integrated strategy that combined QTL-mapping with expression profile analysis, six candidate genes, which were up-regulated under chilling stress at the seedling and booting developmental stages, were studied. The results may help in understanding cold tolerance mechanisms and in using beneficial alleles from wild rice to improve the cold tolerance of rice cultivars through molecular marker-assisted selection.

Characteristics of Traditional Chinese Medicine Syndrome in Patients with Coronary Heart Disease at Different Disease Stages

Coronary atherosclerotic heart disease(CHD)has a high incidence,disability rate,and mortality rate,which severely affect people’s health,work,and life.Based on its clinical manifestations,traditional Chinese medicine(TCM)has classified CHD as“chest impediment”and“heart pain”for the treatment.The course of CHD is protracted,and the pathogenesis is complex and changeable.Moreover,different disease stages have different syndrome characteristics.Thus,this article aimed to summarize the classification and distribution characteristics of the TCM syndrome in various stages of CHD,such as before and after percutaneous coronary intervention therapy,disease progression,age,and complications,to provide references for the TCM clinical diagnosis and treatment of CHD.

Effect of Maturity Indices on Growth and Quality of High Value Vegetables

The study took place at Bangladesh Agricultural Research Institute’s Olericulture Division’s research farm from March 2021 to February 2022 (BARI). In a protected net house, we investigated the impact of five different types of vegetables on various maturation stages, including tomato, broccoli, sweet pepper, cucumber, and netted melon. Vegetables cultivated under protected conditions in a transparent poly-film net house can improve quality, maturity, fruit size, and yield. When fruits and vegetables are picked before they are fully mature, they may stay green for longer, but they may not ripen to a satisfactory color and flavor, resulting in a loss of consumer confidence. Furthermore, because fruit continues to grow until the harvest, immature fruit will be smaller than mature fruit, reducing harvest yield. We tried to determine the right maturation stages in order to avoid product loss during our investigation. The tomato was found to be an appropriate size (6.5 cm length and 6.2 cm diameter), weight (84 g), TSS (4.5 percent), pH (4.3), “turning red”, and “tasty” at the week 5 stage, while the broccoli was found to be an appropriate size (12.0 cm length and 13.0 cm diameter), weight (360 g), and “green” color at the week 5 stage. At the week 6 stage, the nettled melon was found to be of appropriate size (15.2 cm length and 14.5 cm diameter), weight (800 g), TSS (10.8 percent), pH (6.3), “net fully developed” on the fruit skin and “much tasty,” while cucumber was found to be of appropriate size (8.8 - 10.8 cm length and 2.2 - 2.9 cm diameter), weight (61 - 88 g), TSS (3.8 - 4.1 percent), pH (6.3), “less powdery”. As a result, establishing the optimal maturity of our research will benefit both consumers and growers.

The Difference Effect of Environmental Regulation on Two Stages of Technology Innovation in China's Manufacturing Industry

This paper divides technological innovation into two stages:technology development and technology transfer.Then the authors use the panel data of 28 manufacturing industries during 2003–2012 to test the econometric regression model for the industry of the sub stages,which is a regulation on technological innovation in the environment.The results show that:(1) environmental regulation has a significant role in promoting China's manufacturing technology research patent achievements,and technology into new products,and this indicated that"Porter hypothesis" in the manufacturing sector has been verified;(2) R&D and transfer expenditure have a positive impact on technological innovation.Finally,the authors put forward the corresponding policy recommendations for industry of the environmental regulation on the impact of technological innovation in phases.

Impact of Chinese Local Government-led Construction Land Supply Strategies on Urban Innovation and its Spatiotemporal Differences

This study constructs a theoretical framework to analyze the impact of local governmentled construction land supply(LGCLS)strategies on urban innovation.It uses data related to 265 cities in China from 2005 to 2019 for empirical analysis.Its conclusions are as follows.For development stage differences,the impact of LGCLS strategies on urban innovation exhibited an inverted U shape with economic growth.The inflection point occurred when the per capita GDP equaled approximately RMB23,101 in 2008.For regional diferences,the inflection point in eastern China is higher and earlier than that in central and western China;before the inflection point,the positive infuence was more obvious in the central and western regions,whereas after the inflection point,the negative influence was more obvious in the eastern region.For the eastern Yangtze River Delta urban agglomerations and the northwestern resource-based urban agglomerations with relatively high per capita GDP,the negative impact of LGCLS strategies on urban innovation occurred earlier and showed spatial agglomeration characteristics.

徐志瑛教授治疗慢性阻塞性肺疾病经验

徐志瑛教授认为,慢性阻塞性肺疾病多因久病肺气虚所致,气虚基础上逐渐发展为气虚、气阴两虚为其本,痰瘀交阻、气机升降失常贯穿疾病始终为其标,病位在肺,可涉他脏,临证应分期辨证,治疗上应重视"痰"、"瘀"、"虚"产生的机理,从肺脾肾三脏分期治疗,达到临床缓解,提高患者生活质量。