A new method called differential reciprocal oscillographic chronopotentiometric titration is presented.It makes use of appearance or disappearance of a peak of depolarizer on differential reciprocal oscillogram indica...A new method called differential reciprocal oscillographic chronopotentiometric titration is presented.It makes use of appearance or disappearance of a peak of depolarizer on differential reciprocal oscillogram indicating the end point of titration.This method was applied to titration of Pb^(2+),Cd^(2+),Ni^(2+),Co^(3+),Zn^(2+),Ga^(3+) and Cu^(2+)by EDTA or EGTA.The end point of titration is very easy to observe and the sensibility of the method is higher than that of diffcrcntial oscillographic chronopotcntiometric titration.展开更多
The fetal neocortical transplant (E15-17 days gestation) of Wistar rat was grafted to the corresponding neocortical region (frontal-parietal lobe) of the same strain in young rats (4-5 weeks old). On the 7th, 15th, 30...The fetal neocortical transplant (E15-17 days gestation) of Wistar rat was grafted to the corresponding neocortical region (frontal-parietal lobe) of the same strain in young rats (4-5 weeks old). On the 7th, 15th, 30th, 60th, 150th day after transplantation, the sections cut through the middle area of graft-ost brain were examined by HE, Nissl, Glees stain, immunohistochemical technique for GFAP and NF, Nissl, Glees stain, immunohistochemical technique for GFAP and NF, acetylcholinesterase (AChE) histochemistry as well as horseradish peroxidase (HRP) retrograde tracing with light microscope. Some of the sections were also examined with TEM. The result showed that most immature neurons within the graft can survive, grow, differentiate and mature, and are similar to the structure of the neocortical neurons of host brain. This study also provides patterns of integration of the interface between graft-host brain varying with the proliferation of reactive astrocyte as well as graft-host reciprocal connection of fibers.展开更多
文摘A new method called differential reciprocal oscillographic chronopotentiometric titration is presented.It makes use of appearance or disappearance of a peak of depolarizer on differential reciprocal oscillogram indicating the end point of titration.This method was applied to titration of Pb^(2+),Cd^(2+),Ni^(2+),Co^(3+),Zn^(2+),Ga^(3+) and Cu^(2+)by EDTA or EGTA.The end point of titration is very easy to observe and the sensibility of the method is higher than that of diffcrcntial oscillographic chronopotcntiometric titration.
文摘The fetal neocortical transplant (E15-17 days gestation) of Wistar rat was grafted to the corresponding neocortical region (frontal-parietal lobe) of the same strain in young rats (4-5 weeks old). On the 7th, 15th, 30th, 60th, 150th day after transplantation, the sections cut through the middle area of graft-ost brain were examined by HE, Nissl, Glees stain, immunohistochemical technique for GFAP and NF, Nissl, Glees stain, immunohistochemical technique for GFAP and NF, acetylcholinesterase (AChE) histochemistry as well as horseradish peroxidase (HRP) retrograde tracing with light microscope. Some of the sections were also examined with TEM. The result showed that most immature neurons within the graft can survive, grow, differentiate and mature, and are similar to the structure of the neocortical neurons of host brain. This study also provides patterns of integration of the interface between graft-host brain varying with the proliferation of reactive astrocyte as well as graft-host reciprocal connection of fibers.
