一种基于Filter Faster R-CNN的数字PCR液滴检测技术

目的研究液滴数字聚合酶链式反应(ddPCR)液滴检测技术,去除图像中灰尘、气泡、芯片表面的划痕以及微小凹陷等因素产生的异常点对结果的影响,实现高通量、稳定和准确的ddPCR液滴的自动检测。方法提出Filter Faster R-CNN ddPCR液滴检测模型。使用Faster R-CNN生成液滴预测框,之后使用异常点过滤模块(Filter)去除阳性液滴预测框中的异常点。以诺如病毒片段的质粒为模板进行ddPCR实验,建立一个ddPCR数据集,用于模型的训练(2462例,约占78.56%)和测试(672例,约占21.44%)。对异常点过滤模块的3个过滤支路在验证集上进行消融实验,通过与其他ddPCR液滴检测模型进行比较的对比实验以及进行ddPCR的绝对定量实验。结果在少尘和多尘的环境中,Filter Faster R-CNN阳性液滴准确率为98.23%和88.35%,综合指标F1分数分别达到了99.15%和99.14%,高于其他相比较的模型。独立样本T检验的结果证明,相比未添加过滤模块的网络,添加过滤模块后能够显著提示模型在多尘环境中的阳性准确率。在ddPCR绝对定量实验中,将商业化流式检测设备的结果作为标准浓度,绘制了回归线。结果显示,回归线斜率为1.0005,截距为-0.025,决定系数达到了0.9997,二者结果高度一致。结论本文提出了一种基于Filter Faster R-CNN的ddPCR液滴检测技术,为在多种环境条件下的ddPCR实验提供了鲁棒的液滴检测方法。

Robust state-space realizations of digital filters against finite word length errors

This article deals with two important issues in digital filter implementation： roundoff noise and limit cycles. A novel class of robust state-space realizations, called normal realizations, is derived and characterized. It is seen that these realizations are free of limit cycles. Another interesting property of the normal realizations is that they yield a minimal error propagation gain. The optimal realization problem, defined as to find those normal realizations that minimize roundoff noise gain, is formulated and solved analytically. A design example is presented to demonstrate the behavior of the optimal normal realizations and to compare them with several well-known digital filter realizations in terms of minimizing the roundoff noise and the error propagation.

Optimal parameter choice of CR–RC^m digital filter in nuclear pulse processing

CR–RCm filters are widely used in nuclear energy spectrum measurement systems. The choice of parameters of a CR–RCm digital filter directly affects its performance in terms of energy resolution and pulse count rate in digital nuclear spectrometer systems. A numerical recursive model of a CR differential circuit and RC integration circuit is derived, which shows that the shaping result of CR–RCm is determined by the adjustment parameter (k, it determines the shaping time of the shaper) and the integral number (m). Furthermore, the amplitude– frequency response of CR–RC^m is analyzed, which shows that it is a bandpass filter;the larger the shaping parameters (k and m), the narrower is the frequency band. CR–RC^m digital Gaussian shaping is performed on the actual sampled nuclear pulse signal under different shaping parameters. The energy spectrum of 137Cs is measured based on the LaBr3(Ce) detector under different parameters. The results show that the larger the shaping parameters (m and k), the closer the shaping result is to Gaussian shape, the wider is the shaped pulse, the higher is the energy resolution, and the lower is the pulse count rate. For the same batch of pulse signals, the energy resolution is increased from 3.8 to 3.5%, and the full energy peak area is reduced from 7815 to 6503. Thus, the optimal shaping parameters are m -3 and k -0.95. These research results can provide a design reference for the development of digital nuclear spectrometer measurement systems.

Optimal design study of high order FIR digital filters based on neural network algorithm

An optimal design approach of high order FIR digital filter is developed based on the algorithm of neural networks with cosine basis function . The main idea is to minimize the sum of the square errors between the amplitude response of the desired FIR filter and that of the designed by training the weights of neural networks, then obtains the impulse response of FIR digital filter . The convergence theorem of the neural networks algorithm is presented and proved, and the optimal design method is introduced by designing four kinds of FIR digital filters , i.e., low-pass, high-pass, bandpass , and band-stop FIR digital filter. The results of the amplitude responses show that attenuation in stop-bands is more than 60 dB with no ripple and pulse existing in pass-bands, and cutoff frequency of passband and stop-band is easily controlled precisely .The presented optimal design approach of high order FIR digital filter is significantly effective.

Satisfactory Optimization Design of IIR Digital Filters

A new method called satisfactory optimization method is proposed to design IIR (Infinite Impulse Response) digital filters, and the satisfactory optimization model is presented. The detailed algorithm of designing IIR digital filters using satisfactory optimization method is described. By ~using quantum genetic algorithm characterized by rapid convergence and good global search capability, the satisfying solutions are ~achieved in the experiment of designing lowpass and bandpass IIR digital filters. Experimental results show that the performances of IIR filters designed by the introduced method are better than those by traditional methods.

Demodulation System for Fiber Bragg Grating Sensors Using Digital Filtering Technique

A discrimination measurement method and demodulation technique for fiber Bragg grating (FBG) sensors were presented using digital filtering technique. The system can control a tunable fiber Fabry-Perot filter with sawtooth wave voltage generated by digital clock to interrogate FBG sensors. Using the analogue digital converter (ADC), the reflected FBG signals were sampled with synchronous digital clock. With the aid of digital matched filtering technique, the sampled FBG signals were processed to obtain the maximum signal-to-noise ratio (SNR) and the Bragg wavelength shift from the FBG signals was recovered. The results demonstrate that this system has a scanning range of 1 520 nm-1 575 nm,and the wavelength detection accuracy is less than 2 pm with 1.5 Hz scanning frequency.

A New Design Method for Variable Digital Filter Based on Field Programmable Gate Array(FPGA)

In order to obtain variable characteristics,the digital filter's type,number of taps and coefficients should be changed constantly such that the desired frequency-domain characteristics can be obtained.This paper proposes a method for self-programmable variable digital filter(VDF) design based on field programmable gate array(FPGA).We implement a digital filter system by using custom embedded micro-processor,programmable finite impulse response(P-FIR) macro module,coefficient-loader,clock manager and analog/digital(A/D) or digital/analog(D/A) controller and other modules.The self-programmable VDF can provide the best solution for realization of digital filter algorithms,which are the low-pass,high-pass,band-pass and band-stop filter algorithms with variable frequency domain characteristics.The design examples with minimum 1 to maximum 32 taps FIR filter,based on Modelsim post-routed simulation and onboard running on XUPV5-LX110T,are provided to demonstrate the effectiveness of the proposed method.

Design of twodimensional digital filters using neural networks

A new approach for the design of two-dimensional （2-D） linear phase FIR digital filters based on a new neural networks algorithm （NNA） is provided. A compact expression for the transfer function of a 2-D linear phase FIR filter is derived based on its frequency response characteristic, and the NNA, based on minimizing the square-error in the frequency-domain, is established according to the compact expression. To illustrate the stability of the NNA, the convergence theorem is presented and proved. Design examples are also given, and the results show that the ripple is considerably small in passband and stopband, and the NNA-based method is of powerful stability and requires quite little amount of computations.

NUMERICAL EXPERIMENTS AND ANALYSIS OF DIGITAL FILTER INITIALIZATION FOR WRF MODEL

Initialization and initial imbalance problem were discussed in the context of a three-dimensional variational data assimilation system of the new generation"Weather Research and Forecasting Model". Several options of digital filter initialization have been tested with a rain storm case. It is shown that digital filter initialization, especially diabatic digital filter initialization and twice digital filter initialization, have effectively removed spurious high frequency noise from initial data for numerical weather prediction and produced balanced initial conditions. For six consecutive intermittent data assimilation cycles covering a 3-day period, mean initialization increments and impact on forecast variables are studied. DFI has been demonstrated to provide better adjustment of the hydrometeors and vertical velocity, reduced spin-up time, and improved forecast variables quantity.

Digital Filter Algorithm Based on Morphological Lifting Scheme and Median Filter

When the circuits in which electronic products are fitted are disturbed by various interrupting signals, wave distortions occur to the normal voltage signals of these circuits.These wave distortions influence the normal operation and life cycle of electronic products. To eliminate the harmful effects of interrupting signals on electronic products, in this paper, a digital filter algorithm based on morphological lifting scheme and median filter(MLS-MF), which will be used to filter various interrupting signals existing in the circuits in which electronic products are fitted, is proposed. A variety of interrupting signals have been included in simulation studies, and simulation results have demonstrated the effectiveness and feasibility of the proposed digital filter algorithm in high frequency continuous interference, random background noise and damped oscillatory transient interference filter.

On low-pass digital filters in oceanography

-Two types of filters are widely used to remove semidirunal and diurnal tidal signals and other high frequency noises in oceanography. The first type of filters uses moving average with weights in time domain, and can be easily operated. Some data will be lost at each end of the time series, especially for the low low-pass filters. The second type of filters uses the discrete Fourier transform filter (DFTF) which operates in the frequency domain, and there are no data loss at the ends for the forward transform. However, owing to the Gibbs phenomenon and the discrete sampling (Nyquist effect) , ringing appears in the inverse transformed data, which is especially serious at each end. Thus some data at the ends are also discarded. The present study tries to find out what causes the ringing and then to seek for methods to overcome the ringing. We have found that there are two kinds of ringings, one is the Gibbs phenomenon, as defined before. The other is the 'Nyquist'ringing due to sampling Nyquist critical frequency. The former is due to the abrupt transition in frequency band. The Gibbs and Nyquist effects show the ringing at each end of the filtered time series. Thus, the use of a cosine taper or a linear taper on the window in the frequency domain makes the transition band smooth, so that the Gibbs phenomenon will be minimized. Before applying the Fast Fourier Transform (FFT), the original time series at each end is properly tapered by a split cosine bell that reduces significant ringing since this method limits the energy transfer from outside of the Nyquist frequency. Thus, the DFTF can be a powerful tool to suppress the signals in which we are not interested, with sharp peaks in low frequency variation and less data loss at each end.

DESIGN OF DIGITAL IMPACTING FILTER IN CP-EBPSK WITH RANDOM-POLAR COMMUNICATION SYSTEM

To solve the difficulty of designing digital impacting filter in the receiver of random-polar modulated Extended Binary Phase Shift Keying with Continuous Phase (CP-EBPSK), a design method based on Quantum-behaved Particle Swarm Optimization (QPSO) algorithm is proposed. Firstly, QPSO is introduced elaborately, and the basic flow of QPSO is also given. Then, the demodulation principle of digital impacting filter in the communication system of CP-EBPSK with random-polar is demonstrated, and QPSO is utilized to design the digital impacting filter, which also takes the effect of finite word length into consideration when implemented by hardware. Finally, the proposed method is simulated. Simulation results show that the digital impacting filter designed by new method can derive satisfied demodulation performance.

Improvement of digital S-K filter and its application in nuclear signal processing

On the basis of preliminary studies,a novel duo-parameter model consisting of amplitude filter factor and frequency filter factor for low-pass S-K filter is presented in this paper.The model is established by applying numerical differentiation method.Some simulation experiments and real data tests are carried out to verify the feasibility and superiority of the new algorithm.The results show that this duo-parameter model of low-pass S-K filter can be used to achieve high performance in signal processing and nuclear spectrum smoothing.

AN ITERATIVE ALGORITHM FOR OPTIMAL DESIGN OF NON-FREQUENCY-SELECTIVE FIR DIGITAL FILTERS

This paper proposes a novel iterative algorithm for optimal design of non-frequency-selective Finite Impulse Response(FIR) digital filters based on the windowing method.Different from the traditional optimization concept of adjusting the window or the filter order in the windowing design of an FIR digital filter,the key idea of the algorithm is minimizing the approximation error by succes-sively modifying the design result through an iterative procedure under the condition of a fixed window length.In the iterative procedure,the known deviation of the designed frequency response in each iteration from the ideal frequency response is used as a reference for the next iteration.Because the approximation error can be specified variably,the algorithm is applicable for the design of FIR digital filters with different technical requirements in the frequency domain.A design example is employed to illustrate the efficiency of the algorithm.

Simulation of Cellular Neural Networks by Wave Digital Filter Principles

Based on wave digital filter(WDF) principles, this paper presents a digital model of cellular neural networks(CNNs). The model can precisely simulate the dynamic behavior of CNNs.

Digital Imaging Reconstruction from Multiple Angle Diversity Using Digital Filtering Technique

Microwave diffraction tomography is a process to infer the internal structure of an objectfrom multiple angle views of microwave diffraction shadow. Being sensitive to variations in refractive index of the object, the procedure can be used to measure permittivity distributions within dielectric objects and to image soft tissues for biomedical applications. The optimal resolution distance obtainable is half a wavelength, but this can rarely be achieved because of practical limitations. Some procedures, however, are available to improve the practical resolution. One, which is suitable for microwave tomography, is to use multiple angle views data and to combine the resulting images. The other, which is suitable for improving the image reconstruction resolution, is to use the digital filtering technique and the filtered backpropagation algorithm. A system operating over the X-band microwave frequency is described and some experimental results for objects in air are given.

Optimized Implementation for Wave Digital Filter Based Circuit Emulation on FPGA

A binary tree representation is designed in this paper for optimization of wave digital filter（WDF）implementation.To achieve this,an equivalent WDF model of the original circuit is converted into abinary tree representation at first.This WDF binary tree can then be transformed to several topologies with the same implication,since the WDF adaptors have a symmetrical behavior on their ports.Because the WDF implementation is related to field programmable gate array（FPGA）resource usage and the cycle time of emulation,choosing aproper binary tree topology for WDF implementation can help balance the complexity and performance quality of an emulation system.Both WDF-FPGA emulation and HSpice simulation on the same circuit are tested.There is no significant difference between these two simulations.However,in terms of time consumption,the WDF-FPGA emulation has an advantage over the other.Our experiment also demonstrates that the optimized WDF-FPGA emulation has an acceptable accuracy and feasibility.

Polyphase Filter Banks for Embedded Sample Rate Changes in Digital Radio Front-Ends

This paper presents efficient processing engines for software-defined radio （SDR） front-ends. These engines, based on a polyphase channelizer, perform arbitrary sample-rate changes, frequency selection, and bandwidth control. This paper presents an M-path polyphase filter bank based on a modified N-path polyphase filter. Such a system allows resampling by arbitrary ratios while performing baseband aliasing from center frequencies at Nyquist zones that are not multiples of the output sample rate. This resampling technique is based on sliding cyclic data load interacting with cyclic-shifted coefficients. A non-maximally-decimated polyphase filterbank （where the number of data loads is not equal to the number of M subfilters） processes M subfilters in a time period that is less than or greater than the M data loads. A polyphase filter bank with five different resampling modes is used as a case study for embedded resamp/ing in SDR front-ends. These modes are （i） maximally decimated, （ii） Under-decimated, （iii） over-decimated, and combined up- and down-sampling with （iv） single stride length, and （v） multiple stride lengths. These modes can be used to obtain any required rational sampling rate change in an SDR front-end based on a polyphase channelizer. They can also be used for translation to and from arbitrary center frequencies that are unrelated to the output sample rates.

DESIGN OF POLYPHASE WAVE DIGITAL FILTERS WITH APPROXIMATELY LINEAR PHASE

It is well known that IIR digital filters require quite fewer computations,comparedwith FIR filters,in order to meet stringent magnitude specifications when the phase distortioncan be tolerated.An approximately linear phase,however,can be also obtained with the IIRfilter by making use of a technique without increasing the complexity.Based on a certain numberof attenuation zeros in the pass band,a new approach is developed for the design of polyphasewave digital filters with exact magnitude responses and Chebyshev approximation of the desiredphase responses.The minimum number of attenuation zeros is estimated,and some examples areincluded.

SIMPLIFIED LATTICE REALIZAT ION OF FIR DIGITAL FILTERS

This paper suggests a simplified lattice structure for implementing FIR digital filters,which halvesthe hardware requirements of a conventional lattice realization.The conversion algorithms between directrealizations and the simplified lattice forms are given.Furthermore,the algorithms for linear phase FIR fil-ters are simplified.The principal results are illustrated by a practical example.