A Paraffin Sectioning Method Without Using the Microtome and an Analysis Method for Optical Information of the Plant Anatomical Digital Photographs

[Objective] The research aimed to study a paraffin method without using the microtome,and also introduced an analysis method for optical information of the plant anatomical digital photographs.[Method] The plant material softened or not was embedded in paraffin according to the paraffin method.Cut the thin paraffin sections from the paraffin block with a sharp two-sided blade under anatomical lens.The thin material sections rolled up when they were cut off.Took the section rolls to a slide,and then heated them to melt the paraffin section roll.When the paraffin melted,the sections of plant material were rolled out.After the common or simplified procedures of staining and mounting,the preparations were finished.When an anatomical digital photograph was processed,copy it into the word file and two copies of the original photograph were obtained.One copy was selected to make it to be a negative photograph,and then press the key ＂Press Screen＂ to copy the screen frame.After it was copied into the word file,cut of the unnecessary parts and other operations were carried out,then processed photograph was obtained.[Results] The anatomical preparation for research was gotten.The analyzed digital photograph of the leaf structure of Salix matsudana var.matsudana f.tortuosa has some a three-dimensional effect,and the different leaf structures and cells,e.g.cuticle,cell wall,protoplast,vein,etc.can be identified easily.[Conclusion]The paraffin method without using the microtome has advantages of low cost and higher efficiency,which could be applied by the beginner or in the time without a microtome to be used.The analysis of the plant anatomical digital photographs can acquire more structural information than the original digital photographs,which shows the potentiality and prospects of the optical information analysis of the microscopic imagery and its digital photograph.

Photo Socialization in Digital Natives

In the following work of research, we shall present the results of a study on forms of digital photographic socialization as carried out by the so-called ＂digital natives＂ in order to explore their competence in managing digital photographic output and cultural practices. This shall be done under the following categories of analysis： snapshot, organization, socialization, reception, privacy, and copyrights in digital photography. The study explores the forms of digital photography production, technical problems of photographic management and the final uses that digital photographers give to their photos in terms of web tools 2.0, mobile telephones and social networks. Digital photography technology, compact cameras, mobile phones with cameras, specialized websites in photography and the lnternet generate new forms of production and emerging styles of digital photographic socialization. The study is carried out using a defined sample of university students, those digital natives who create and use digital photography.