镉诱导威廉环毛蚓（Pheretima guillelmi）金属硫蛋白的分离纯化及特性研究

蚯蚓威廉环毛（ｐｈｅｒｅｔｉｍａ ｇｕｉｌｌｅｌｍｉ）经皮下注射ＣｄＣｌ２溶液诱导后，整体匀浆，再经热沉淀、乙醇沉淀后，经凝胶过滤Ｓｅｐｈａｄｅｘ Ｇ－５０柱层析，得两个镉结合蛋白峰，分子量依次为４３ｋＤ及１９ｋＤ。这两个组份再分别经ＤＥＡＥ Ｓｅｐｈａｒｏｓｅ Ｆａｓｔ Ｆｌｏｗ柱层析，各得三个镉结合蛋白峰。根据光谱学特征、巯基含量及氨基酸组成等分析，表明凝胶过滤第二峰为金属硫蛋白（ＭＴ），其经ＤＥＡＥ柱层析所得三个峰为ＭＴ三个亚型，Ｎ末端分别为Ｌｙｓ、Ａｌａ、Ａｌａ。

秉氏环毛蚓(Pheretima pingi Stephenson)雄性生殖细胞减数分裂

以秉氏环毛蚓雄性生殖细胞为实验材料,通过石蜡连续切片方法,对雄性生殖细胞的减数分裂进行了初步研究,并分折了雄性生殖细胞在精巢囊、贮精囊中从形成到成熟的过程。进一步了解秉氏环毛蚓的染色体数2n=8。这为促进蚯蚓生物学研究、人工养殖和综合利用,以及蚯蚓的远缘杂交和育种选种提供理论依据。

威廉环毛蚓[Pheretima guillelmi(Michaelsen)]前列腺显微及亚显微结构的研究

环毛属(Pheretima)是我国常见的和优势的属,其中大多数的种类具有发达的前列腺,该腺属于雄性生殖器.威廉环毛蚓的前列腺由小叶组成,每小叶内有一至几个分泌小管.前列腺腺细胞及其核的形态多样。腺细胞的分泌功能旺盛,在细胞质中有很多分泌颗粒、大量的粗面内质网和高尔基体。前列腺腺细胞中的分泌颗粒与蚯蚓精子的营养和活动有关,可能与其成熟的程度也有关。前列腺的分泌活动可能还与季节有一定的关系,因为在生殖季节里比较旺盛.

用COI基因确定直隶环毛蚓(Pheretima tschiliensis tschiliensis)的属的地位

目的：由于采用新的分类体系,国内外学者通过形态学特征对直隶环毛蚓（Pheretima tschiliensis tschiliensis,Michealson,1928）的分类地位一直存在争议,该文的目的在于将此种给予明确的划分。方法：用采自不同地区的直隶环毛蚓COI基因（534bp）及结合其相应的氨基酸（178个）,以陆正蚓（Lumbricus tereistial）为外群,用最大简约法和贝叶斯法构建进化树的方法,结果：试验结果表明,构建的系统树将直隶环毛蚓与腔蚓属聚为一族,并得到很好的支持（BP〉76,pp≥0.94）,种间校正基因距离介于0.006-0.263,种内校正基因距离介于0.000-0.162,结论：因具有交配腔,故分子生物学和形态学特征均表明,直隶环毛蚓应属于腔蚓属而不是远盲蚓属。其学名应为Metaphire tschiliensis tschiliensis（Michealson,1928）。

Effect of lead on survival, locomotion and sperm morphology of Asian earthworm, Pheretima guillelmi

To provide basic toxicity data for formulating risk characterization benchmarks, the effects of lead on survival, locomotion, and sperm morphology were investigated in the Asian earthworm Pheretima guillelmi. The LC50 of P. guiUelmi for 7 and 14 d were 4285＋339 mg/kg and 3207＋248 mg/kg, which shows P. guillelmi can tolerate a higher concentration of lead nitrate. The average weight of the surviving earthworms decreased at concentration of 2800 mg Pb/kg soil, and the locomotor ability of earthworms exposed to a range of soil Pb concentrations showed a general decrease with increasing Pb concentrations. We also presented data depicting the sperm morphology of earthworms, which shows potential as a sensitive biomarker for measuring the effects of heavy metal on reproduction.

Electrochemical Assay of Effects of Organophosphate Poisoning on Acetylcholinesterase from Pheretima via 2,6-Dimethyl-p-benzoquinone

Electroanalytical techniques could be a reliable and promising alternative to classical and sophisticated methods because of their simplicity（small and portable）,easy use,the ability to deliver fast response with high sensitivity and selectivity.A square wave voltammetric method was developed for the assessment of organophosphorus（OPs） compound impact on acetylcholinesterase（AChE） of Pheretima with 2,6-dimethyl-p-benzoquinone（2,6DMBQ） as a redox indicator.The substrate of acetylthiocholine is hydrolyzed by AChE and the produced thiocholine reacts with 2,6-DMBQ to give an obvious shift of electrochemical signal.The reduction peak of 2,6-DMBQ is located at around 0.18 V which is far away from the oxidation potential of possible interference components often present in biosample.The decreased rate of reduction current was related with the activity of AChE.The inhibition of parathion-methyl on AChE was assessed.The inhibiton rate of OPs on AChE activity increased quickly during the first 10 min inhibition,and after that the value of inhibition rate approached to be constant.AChE lost almost 29.3% of activity after 10 min incubation with 1 μg/mL parathion-methyl and 67.5% of activity with 10 μg/mL parathion-methyl,while the activity that corresponds to 40 μg/mL parathion-methyl was nearly completely inhibited（94.9%）.Compared to cyclic voltammetry and amperometry,Square wave voltammetry（SWV） method is a high sensitive electroanalysis with fast scan-rate（only several seconds for one signal value） which is useful to prevent the electrodes from possible fouling or passivation.This method can be employed to assess the inhibition of organophosphate on AChE and investigate OPs impact on environmental animals.

Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics

Pheretima,also called“earthworms”,is a well-known animal-derived traditional Chinese medicine that is extensively used in over 50 Chinese patent medicines(CPMs)in Chinese Pharmacopoeia(2020 edition).However,its zoological origin is unclear,both in the herbal market and CPMs.In this study,a strategy for integrating in-house annotated protein databases constructed from close evolutionary relationship-sourced RNA sequencing data from public archival resources and various sequencing algorithms(restricted search,open search,and de novo)was developed to characterize the phenotype of natural peptides of three major commercial species of Pheretima,including Pheretima aspergillum(PA),Pheretima vulgaris(PV),and Metaphire magna(MM).We identified 10,477 natural peptides in the PA,7,451 in PV,and 5,896 in MM samples.Five specific signature peptides were screened and then validated using synthetic peptides;these demonstrated robust specificity for the authentication of PA,PV,and MM.Finally,all marker peptides were successfully applied to identify the zoological origins of Brain Heart capsules and Xiaohuoluo pills,revealing the inconsistent Pheretima species used in these CPMs.In conclusion,our integrated strategy could be used for the in-depth characterization of natural peptides of other animal-derived traditional Chinese medicines,especially non-model species with poorly annotated protein databases.

Xiongzhi Dilong decoction interferes with calcitonin gene-related peptide(CGRP)-induced migraine in rats through the CGRP/iNOS pathway

Objective: To evaluate the effects of Xiongzhi Dilong decoction(XZDLD) and its wind medicine on calcitonin gene-related peptide(CGRP)-induced migraine and explore the mechanism through the CGRP/inducible nitric oxide synthase(iNOS) pathway.Methods: Rats were divided into control, model, XZDLD, XZDLD(external wind), XZDLD(internal wind),and olcegepant groups. CGRP was injected into the dura mater to induce a migraine. The frequency of head scratching, cage climbing, and facial grooming was observed. The pain threshold, the levels of CGRP,pituitary adenylate cyclase activating polypeptide(PACAP), substance P(SP), and the plasma protein extravasation(PPE) ratio were measured. The phosphorylation levels of p38, extracellular signalregulated kinase 1/2(ERK1/2), and expression of iNOS were detected by western blot.Results: Compared with the model group, the three modified XZDLD groups showed reduced frequency of head scratching and cage climbing in the first 30 min(all P <.05). Facial grooming frequency was reduced in XZDLD and XZDLD(external wind) groups(P =.0003 and P =.0131, respectively). External wind medicine played a more important role in increasing mechanical pain threshold than internal wind medicine. Moreover, compared with the model group, the three modified XZDLD groups demonstrated reduced plasma levels of CGRP and PACAP(all P <.05). No difference in the SP level was observed among the six groups. XZDLD reduced PPE ratio. XZDLD and XZDLD(external wind) groups suppressed the CGRP/iNOS pathway by inhibiting the p-p38/p38 ratio and the expression of iNOS. No difference in pERK1/2/ERK1/2 ratio was detected among the six groups.Conclusion: XZDLD increases pain threshold, downregulates the expression of CGRP and PACAP, and reduces PPE ratio by inhibiting the CGRP/iNOS pathway. External wind medicine is more effective than internal one on improving facial grooming and head scratching, increasing the mechanical pain threshold, and inhibiting the expression of iNOS.

Protective Effect and Molecular Mechanism of Shui People's Classic Prescription Jipei Dilong Ointment on Osteoarthritis in Rats

[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control group,model group,Jipei Dilong Ointment high,medium and low dose groups,and positive drug Diclofenac group,with 12 rats in each group.Except the control group,all other groups were injected with 0.2 mL of 2%papain and 0.3%L-cysteine mixture into knee joint cavity to establish osteoarthritis model,while the control group was injected with the same amount of normal saline.The Lequesne MG score was used to determine the success of the model.After successful modeling,external administration was given for 4 weeks.The histopathological changes in articular cartilage and synovium were observed by HE staining;the levels of TNF-α,IL-1βand COX-2 in serum were detected by ELISA,and the relative expression of MMP-9 and TIMP-1 mRNA in cartilage was detected by qRT-PCR;the relative expression of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB in rat cartilage was detected by Western-blot.[Results]Compared with the model group,Jipei Dilong Ointment could significantly reduce the Lequesne MG score and Mankin s score of arthritic rats(P<0.01);significantly improve the pathological changes in articular cartilage and synovium,reduce tissue edema,necrosis,inflammatory cell infiltration and fibrous tissue proliferation;reduce the expression of MMP-9 mRNA(P<0.01)in different degrees and increase the expression of TIMP-1 mRNA(P<0.01);reduce the relative expression of MMP-9,TLR4,MyD88 and NF-κB protein in different degrees(P<0.01),and significantly increase the relative expression of TIMP-1 protein in cartilage(P<0.01).[Conclusions]Jipei Dilong Ointment can improve the joint injury of osteoarthritis rats,and the mechanism may be related to the regulation of TLR4/MyD88/NF-κB signaling pathway and the ratio of MMP-9/TIMP-1.

威廉环毛蚓Pheretima guillelmi（Michaelsen）...

环毛属(Pheretima)是我国常见的和优势的属,其中大多数的种类具有发达的前列腺,该腺属于雄性生殖器.威廉环毛蚓的前列腺由小叶组成,每小叶内有一至几个分泌小管.前列腺腺细胞及其核的形态多样。腺细胞的分泌功能旺盛,在细胞质中有很多分泌颗粒、大量的粗面内质网和高尔基体。前列腺腺细胞中的分泌颗粒与蚯蚓精子的营养和活动有关,可能与其成熟的程度也有关。前列腺的分泌活动可能还与季节有一定的关系,因为在生殖季节里比较旺盛.

Therapeutic Effect and Mechanism of Jipei Dilong Ointment on Acute Soft Tissue Injury in Rats

[Objectives]This study was conducted to observe the therapeutic effect of Jipei Dilong Ointment on rats with acute soft tissue injury caused by heavy objects and to explore its action mechanism.[Methods]Thirty six rats were randomly divided into six groups(control group,model group,high-dose Jipei Dilong Ointment group(JP-H),medium-dose Jipei Dilong Ointment group(JP-M),low-dose Jipei Dilong Ointment group(JP-L)and diclofenac group).Except for the Control group,other groups were subjected to modeling of acute soft tissue injury by the weight impact method.All administration was performed once a day for nine consecutive days.The local appearance score and activity disorder score were determined after soft tissue injury in rats.HE staining was used to detect the pathological changes of injured soft tissues in rats.RT-PCR was used to detect the relative mRNA expressions of Bax,Bcl-2,MMP-9 and TIMP-1 in injured soft tissues of rats.Western Blot was used to detect the protein expressions of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB p65 in injured soft tissues of rats.Results were statistically analyzed.[Results]Compared with the model group,Jipei Dilong Ointment could significantly improve the appearance symptoms such as swelling and ecchymosis in the injured area and the movement function of the affected limb(P<0.05).It could also improve the infiltration of inflammatory cells and widening of the intermuscular space caused by injury.Among them,the JP-H group and the diclofenac group had more significant curative effects.After 9 d of administration,each administration group could significantly up-regulate the ratio of Bcl-2/Bax mRNA expression level(P<0.05 or P<0.01),and the ratio of MMP-9/TIMP-1 mRNA expression level showed a downward trend(P>0.05).The expression level of NF-κB p65 protein in each administration group was significantly decreased(P<0.01).The protein expression levels of TLR4 and MyD88 and the ratio of MMP-9/TIMP-1 protein expression level in each administration group decreased to varying degrees.Among them,the JP-H group and diclofenac group significantly decreased(P<0.05).[Conclusions]Jipei Dilong Ointment has the functions of relieving pain,swelling and inflammation.It could improve the local appearance,functional activity and tissue morphology of affected limbs in rats,and has a therapeutic effect on acute soft tissue injury in rats.Its mechanism of action might be related to the inhibition of TLR4/MyD88/NF-κB p65 signaling pathway and the regulation of Bcl-2/Bax and MMP-9/TIMP-1 balance.

超高效液相色谱-串联质谱法同时检测地龙药材中4种黄曲霉毒素含量及阳性结果确证

建立超高效液相色谱-串联质谱法同时测定地龙药材中黄曲霉毒素B1、B2、G1、G2的含量,并对阳性结果进行确证.样品经70%甲醇提取、免疫亲和柱净化、超高效液相色谱柱分离、三重四极杆质谱检测,并采用离子峰度比进行阳性结果确证.结果表明,4种黄曲霉毒素的线性关系良好(r>0.9995),回收率在91.2%~95.6%范围内.黄曲霉毒素B1、B2、G1、G2的检出限分别为0.10、0.038、0.11、0.038µg/kg.方法专属性好,灵敏度高,准确性好,可以进行最终阳性检出的判定.15批地龙药材中6批黄曲霉毒素确证阳性检出,证明地龙药材较易被黄曲霉毒素污染.

威廉环毛蚓中8种核苷提取工艺优化及含量测定

目的优化威廉环毛蚓中尿嘧啶、次黄嘌呤、黄嘌呤、尿苷、胸腺嘧啶、肌苷、鸟苷、2′-脱氧鸟苷提取工艺,并测定其含量。方法以料液比、超声时间、超声温度为影响因素,次黄嘌呤、总核苷含量为评价指标,正交试验优化提取工艺。HPLC法测定各核苷含量,分析采用Agilent C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-水,梯度洗脱;体积流量1 mL/min;柱温30℃;检测波长260 nm。结果最佳条件为料液比1∶250,超声时间30 min,超声温度60℃。8种核苷在各自范围内线性关系良好(R^(2)>0.9990),平均加样回收率99.11%~103.27%,RSD 0.85%~2.89%。结论该方法稳定可靠,可用于威廉环毛蚓中核苷的提取与含量测定。

威廉环毛蚓提取物蛋白质含量测定方法研究

目的 建立威廉环毛蚓提取物蛋白质含量测定的最佳方法。方法 以氨基酸分析仪测定的威廉环毛蚓提取物中的氨基酸含量为指标,对蛋白质含量检测方法 Lowry法2、改良Lowry法、BCA法、Bradford法进行对比评价。结果 Lowry法2、改良Lowry法、BCA法、Bradford法测定威廉环毛蚓提取物蛋白质含量(上清液)结果分别为27.03%、45.85%、31.58%、11.11%,威廉环毛蚓提取物蛋白质含量(混悬液)的结果分别为38.44%、52.39%、44.06%、11.56%。采用氨基酸分析仪法测定得到威廉环毛蚓提取物中游离氨基酸含量为4.18%,将威廉环毛蚓提取物蛋白质酸水解之后测定总氨基酸含量为48.53%。结论 在威廉环毛蚓提取物样品溶液的处理方式上,与样品上清液相比,样品混悬液的测定结果更接近样品中蛋白质的真实含量。以牛血清白蛋白(BSA)为对照品,相较于Lowry法2、改良Lowry法和Bradford法,BCA法更适宜用于威廉环毛蚓提取物中的蛋白质含量测定。

地龙舒腰汤治疗腰椎间盘突出症椎间孔镜术后残留症状的临床观察

目的观察地龙舒腰汤对腰椎间盘突出症经皮椎间孔镜术后残留症状的临床疗效,为临床上优化治疗及缓解患者症状提供参考。方法将诊断为腰椎间盘突出症并行经皮椎间孔镜术,术后残留腰腿部症状的患者60例,按照随机数字表法分为对照组30例与观察组30例。对照组患者予以常规西药及腰背肌功能锻炼治疗,观察组在对照组的基础上,服用地龙舒腰汤治疗,连续服药14 d。比较2组患者手术前、术后第2周疼痛视觉模拟量表(VAS)、日本骨科学会评估腰椎治疗量表(JOA)及腰椎功能障碍指数量表(ODI)。结果术后第2周2组患者疼痛程度及腰椎功能障碍指数均低于术前,且观察组均低于对照组,差异均具有统计学意义(P<0.05);手术后2组患者JOA评分均高于术前,且观察组高于对照组,差异均具有统计学意义(P<0.05);观察组总有效率为96.70%高于对照组的73.33%,差异具有统计学意义(P<0.05)。结论地龙舒腰汤改善腰椎间盘突出症经皮椎间孔镜术后残留症状有较好疗效。

地龙药材中六溴环十二烷三种异构体残留量的测定和风险评估

目的:建立一种高效液相色谱-串联质谱法(HPLC-MS/MS)同时测定地龙药材中新污染物六溴环十二烷(HBCDs)3种异构体残留量,并评估其健康风险。方法:取地龙药材细粉,加入^(13)C_(12)-α-HBCD、^(13)C_(12)-β-HBCD和^(13)C_(12)-γ-HBCD内标溶液,经超声提取复合硅胶柱净化后,采用液相色谱串联质谱多反应监测,内标法定量。结果:HBCDs 3种立体异构体线性关系良好(r2>0.999),检出限和定量限分别约为2和6 pg·g^(-1),平均回收率为97%~120%,RSD为7.1%~7.4%,精密度为1.1%~7.4%(n=6)。78批地龙药材中有73批检出HBCDs,检出率为94%,其中以α-HBCD的含量最高。使用欧洲食品安全局建议的暴露边界值法对地龙药材中HBCDs的风险评估表明,经地龙药材摄入的HBCDs引起健康问题的风险较小。结论:建立的地龙药材中新污染物HBCDs残留量的液相色谱-串联质谱测定法灵敏、准确、可靠,适用于地龙药材中HBCDs残留量的定量分析,可为中药材外源性新污染物的安全监测提供科学参考。

地龙冬花汤加减治疗儿童哮喘急性发作期合并肺炎支原体感染临床研究

目的:观察地龙冬花汤加减治疗痰浊郁肺型儿童哮喘急性发作期合并肺炎支原体(MP)感染的临床疗效。方法:选取50例痰浊郁肺型哮喘急性发作期合并MP感染患儿,采用随机数字表法分为对照组和治疗组,每组25例。对照组给予阿奇霉素干混悬剂与布地奈德福莫特罗粉吸入剂治疗,治疗组在对照组基础上加用地龙冬花汤加减治疗。2组疗程均为8周。比较2组临床疗效、中医证候积分、呼气峰流速占预计值的百分比(PEF%pred)及第1秒用力呼气容积占预计值的百分比(FEV1%pred)。结果:治疗8周后,治疗组总有效率96.00%,与对照组88.00%比较,差异无统计学意义(P>0.05)。2组中医证候积分均较治疗前降低,治疗组中医证候积分低于对照组,差异均有统计学意义(P<0.05)。2组PEF%pred、FEV1%pred均较治疗前升高,治疗组PEF%pred、FEV1%pred均高于对照组,差异均有统计学意义(P<0.05)。结论:地龙冬花汤加减能够改善痰浊郁肺型哮喘急性发作期合并MP感染患儿的临床症状、体征和肺功能,有效控制哮喘发作。

地龙舒腰汤治疗退行性腰椎管狭窄症(肾虚血瘀型)临床疗效观察

目的:探究地龙舒腰汤治疗退行性腰椎管狭窄症(肾虚血瘀型)的临床疗效。方法:选取2021年12月—2022年12月收治的退行性腰椎管狭窄症患者68例,按随机数字表法将患者分为治疗组与对照组,每组各34例,对照组予以现代医学常规治疗,治疗组在对照组基础上予以地龙舒腰汤治疗,持续治疗28d。比较2组患者治疗前后的疼痛视觉模拟评分法(VAS评分)、日本骨科学会腰椎评估治疗分数评分(JOA评分)及中医证候积分。结果:治疗前,2组患者疼痛程度、腰椎评估治疗分数及中医证候积分比较,差异均无统计学意义;治疗后2组患者疼痛程度及中医证候积分均低于治疗前,且治疗组均低于对照组,差异均具有统计学意义;治疗后2组患者JOA评分均高于治疗前,且治疗组高于对照组,差异具有统计学意义;治疗组有效率为97.06%高于对照组的88.23%,差异具有统计学意义。结论:地龙舒腰汤能有效缓解退行性腰椎管狭窄症患者的临床症状,有效减轻疼痛,改善其腰椎功能,提高患者的生活质量,值得在临床推广。

地龙舒腰汤加味对腰椎间盘突出症后路术后残余根性腰腿痛(气滞血瘀型)临床疗效观察

目的:观察地龙舒腰汤加味治疗腰椎间盘突出症(LDH)后路后残余神经根症状(气滞血瘀型)临床疗效。方法:选取2022年12月—2023年9月腰椎后路椎板减压植骨融合内固定术后残余根性症状(气滞血瘀型)患者60例,按照随机数字表法分为治疗组与对照组,每组30例。术后常规补液、换药预防感染,对照组在此基础上服用塞莱昔布胶囊与甲钴胺片,治疗组在对照组基础上服用地龙舒腰汤加味治疗,连续服用药物4周。比较2组患者在接受治疗后2周、4周、6周的视觉模拟评分法(Visual Analogue Scale,VAS),腰椎JOA(Japanese Orthopaedic Association scores)评分,分析手术单节段与多节段及年龄因素对患者疼痛(VAS)评分改善的影响判定地龙舒腰汤加味采用治法是否有效并评估有效率。结果:治疗后2周、4周、6周治疗组VAS评分明显低于对照组;腰椎JOA评分,治疗组的评分明显高于对照组;2组2周、4周、6周VAS评分,单节段低于多节段评分;治疗组2周、4周、6周,单节段与多节段的评分均低于对照组单节段与多节段的评分;年龄与VAS评分下降分值之间存在负相关,治疗后4周、6周,治疗组VAS下降分值高于对照组;治疗组有效率高于对照组,差异均有统计学意义。结论:地龙舒腰汤加味采用温经通络、活血化瘀兼以补益气血、补益肝肾法,能够有效改善LDH术后腰椎功能,缓解术后根性腰腿疼痛。

地龙有效成分对高糖下HMC细胞炎症因子分泌影响

目的观察地龙有效成分含药血清对HMC细胞炎症因子分泌的影响,并探讨其可能的作用机制。方法地龙有效成分及福辛普利干预高糖刺激HMC模型细胞,并设立正常组和高汤刺激组进行对照,应用MTT法检测HMC细胞生长抑制水平,酶联免疫法检测细胞培养液中TNF-α、IL-6和MCP-1的含量,应用RT-qPCR技术检测细胞样本的TNF-α、IL-6和MCP-1 mRNA表达水平。结果地龙有效成分组、福辛普利组HMC细胞生长平均抑制率下降(P<0.05);地龙有效成分、福辛普利干预后,细胞培养液中的TNF-α、IL-6和MCP-1含量均有下降趋势,与高糖刺激组比较,地龙有效成分组培养液中细胞TNF-αmRNA表达水平下降,MCP-1 mRNA表达水平上升,福辛普利组TNF-α、IL-6 mRNA表达水平明显下降。结论地龙有效成分对高糖下HMC细胞的干预作用可能是抑制细胞炎症因子分泌。