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Amperometric Sensors for Determination of Hydrogen Peroxide Based on Electron Transfer Between Horseradish Peroxidase and 1,1'-Dimethylferrocene
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作者 SUN Chang-qing LUAN Chai-xia +1 位作者 YANG Xu GAO Qian and XU Hong-ding(Department of Chemistry,Jilin University, Changchu,130023)DU Wen-yuan(The National Lab.of Enzyme Engineering,Jilin University, Chanchun, Chanchun,130023) 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 1995年第4期284-290,共7页
hydrogen peroxide electrode with 1,1'-dimethylferrocene(DMFc)used as an electron transfer mediator has been described.Using Nafion, DMFc was modified on a glassy carbon electrode(GCE)surface,and horseradish peroxi... hydrogen peroxide electrode with 1,1'-dimethylferrocene(DMFc)used as an electron transfer mediator has been described.Using Nafion, DMFc was modified on a glassy carbon electrode(GCE)surface,and horseradish peroxidase(HRP)was then immobiliged on the DMFc-Nafion film,forming a HRp-DMFc-Nafion modi- fied electrode. The chracteristics of the sensor has been shown by cyclic voltam- metry and constant potential measurements,The sensor responds fastly to hydro- gen peroxide,the time required to reach 95%of the steady-state current is less than 50s. The sensor displays a sensitive catalytic current response to hydrogen peroxide and can be operated at a potential range in which the oxidation of common interfering species,such as ascorbic acid and uric acid,does not occur. The sensor is stable for 20 days and its detection limit is 1 μmol/L. 展开更多
关键词 dimethylferrocene Nafion Sensors Amperometry Hydrogen peroxide
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