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基于Aβ25-35诱导的PC12细胞损伤模型研究定志小丸治疗阿尔兹海默病的作用机制及配伍机制 被引量:8
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作者 郑妍 刘舒 +1 位作者 宋凤瑞 刘志强 《分析化学》 SCIE EI CAS CSCD 北大核心 2018年第11期1724-1731,共8页
利用β-淀粉样蛋白片段Aβ_(25-35)诱导的PC12细胞损伤模型,从细胞凋亡及氧化应激两个通路研究定志小丸对Aβ_(25-35)引起的PC12神经细胞损伤的保护作用,并将定志小丸拆方为单味药及三味药,用以阐明定志小丸治疗阿尔兹海默病的作用机制... 利用β-淀粉样蛋白片段Aβ_(25-35)诱导的PC12细胞损伤模型,从细胞凋亡及氧化应激两个通路研究定志小丸对Aβ_(25-35)引起的PC12神经细胞损伤的保护作用,并将定志小丸拆方为单味药及三味药,用以阐明定志小丸治疗阿尔兹海默病的作用机制、配伍机制以及主要活性药味。通过MTT和乳酸脱氢酶含量测定评估定志小丸及各味药对PC12细胞活力的影响,其中人参、茯苓效果最好,与模型组相比,可使细胞活力提高约30%。通过细胞凋亡、线粒体膜电位(MMP)、丙二醛(MDA)及还原型谷胱甘肽(GSH)含量测定,进一步从细胞凋亡及氧化应激两个通路研究定志小丸及各味药对Aβ_(25-35)诱导PC12细胞保护作用,研究结果表明,人参、茯苓效果最优,不仅可以显著抑制细胞凋亡,还可降低丙二醛(MDA)含量,减少膜脂过氧化;远志、石菖蒲可以通过提高细胞内还原型谷胱甘肽(GSH)含量进而抑制氧化应激损伤,且与人参、茯苓配伍后,可促进主要成分发挥药效,显著提高人参、茯苓的药效。人参、茯苓是定志小丸中的主要活性药味,远志、石菖蒲起辅助增强药效的作用。 展开更多
关键词 定志小丸 拆方 配伍作用 AΒ25-35 PC12细胞 细胞凋亡 氧化应激
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Modified Tong Xie Yao Fang relieves solitary rectal ulcer syndrome:A case report 被引量:2
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作者 Li-Li Zhang Wan-Shan Hao +2 位作者 Meng Xu Chang Li Yuan-Yuan Shi 《World Journal of Clinical Cases》 SCIE 2019年第15期2058-2064,共7页
BACKGROUND Solitary rectal ulcer syndrome(SRUS)is a rare rectal disorder characterized by bloody mucus in the stool,difficulty in defecation,pain,and anal swelling.To date,the etiology of this syndrome remains not wel... BACKGROUND Solitary rectal ulcer syndrome(SRUS)is a rare rectal disorder characterized by bloody mucus in the stool,difficulty in defecation,pain,and anal swelling.To date,the etiology of this syndrome remains not well understood and the diagnosis is frequently confused with other disorders,making treatment a clinical challenge.CASE SUMMARY A 50-year-old woman presented to our hospital with a 40-d history of bloody mucus in the stool and anal swelling.SRUS was suspected.Rectoscopy revealed a large,severe ulcerous lesion.Histologically,the lesion was characterized as chronic ulcer without clear tumor cells,and the final diagnosis of SRUS was made.The patient was treated with Chinese medicine therapy,with administration of Tong Xie Yao Fang.After 3 wk of treatment,the symptoms improved significantly.At 2-mo follow-up,rectoscopy in a local hospital showed healed ulcer scars without obvious protrusion 3 cm from the anal verge.CONCLUSION Chinese medicine therapy represents a potential treatment of SRUS with predominant rectal bleeding,mucinous discharge,and anal swelling pain. 展开更多
关键词 Solitary rectal ULCER syndrome CHINESE FORMULAS Tong Xie YAO Fang Er SHEN wan ding zhi xiao wan CHINESE medicine therapy Case report
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