A new pregnane glycoside hypoglaucin G was isolated from Dioscorea collettii var. hypoglauca by bioactivity-guided fractionation, which showed a moderate activity of inducing morphological deformation of the Pyricular...A new pregnane glycoside hypoglaucin G was isolated from Dioscorea collettii var. hypoglauca by bioactivity-guided fractionation, which showed a moderate activity of inducing morphological deformation of the Pyricularia oryzae mycelia. The structure of hypoglaucin G was established as 16 beta-(4'-methyl-5'-O-beta-D-glucopyranosyl-pentanoyloxy)-pregn-5-en-3 beta-ol-20-one 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)-]-beta-D-glucopyranoside by chemical evidence and spectral analysis, especially by 2D-NMR techniques.展开更多
采用RAPD分子标记技术对6个叉蕊薯蓣（Dioscorea collettii Hook. f.）居群及5个粉背薯蓣〔D. collettii var. hypoglauca （Palibin） C. T. Ting et al.〕居群的遗传多样性进行了分析,并基于遗传相似系数采用UPGMA法对11个居群进行了...采用RAPD分子标记技术对6个叉蕊薯蓣（Dioscorea collettii Hook. f.）居群及5个粉背薯蓣〔D. collettii var. hypoglauca （Palibin） C. T. Ting et al.〕居群的遗传多样性进行了分析,并基于遗传相似系数采用UPGMA法对11个居群进行了聚类分析。用14个寡聚核苷酸引物共扩增出170条带,其中多态性条带161条,多态性条带百分率高达94.71%;粉背薯蓣5个居群多态性条带百分率的变化幅度（81.25%~89.29%）略大于叉蕊薯蓣6个居群（82.00%~84.21%）。粉背薯蓣居群间的有效等位基因数（Ne）、Nei’s基因多样性指数（h）和Shannon多样性指数（I）分别为1.368 5、0.238 4和0.376 3,叉蕊薯蓣居群间的Ne、h和I分别为1.331 1、0.197 2和0.298 3;叉蕊薯蓣与粉背薯蓣间的基因分化系数（Gst）为0.122 8,基因流（Nm）为3.570 7。二者间的遗传相似系数为0.922 3,遗传距离为0.080 9;其中,粉背薯蓣江西庐山居群和叉蕊薯蓣云南丽江居群间的遗传距离最远,达0.693 1;叉蕊薯蓣云南蒙自和云南景洪居群间的遗传距离最近,仅0.219 4。根据聚类分析结果可将参试的11个居群分成4组,其中,所有的叉蕊薯蓣居群和粉背薯蓣浙江临安居群聚成第1组,粉背薯蓣重庆南川居群和湖南衡山居群聚为第2组,粉背薯蓣湖南永顺居群和江西庐山居群则各自独立成组。研究结果证明,叉蕊薯蓣和粉背薯蓣这2个类群之间为原变种和变种的关系,并存在着变种水平上的分化不完全。展开更多
Rhizoma Dioscoreae Collettii,is known as Huang Dioscorea collettii,Rhizoma Coptidis Brevisepalae or yellow ginger.It is neutral in nature and bitter in taste which is described to Gan(Liver)meridian,Wei(Stomach)meridi...Rhizoma Dioscoreae Collettii,is known as Huang Dioscorea collettii,Rhizoma Coptidis Brevisepalae or yellow ginger.It is neutral in nature and bitter in taste which is described to Gan(Liver)meridian,Wei(Stomach)meridian and Pangguang(Bladder)meridian.It was often used in the treatment of chyloid stranguria,gonorrhea,leucorrhagia,rheumatism arthralgia pain,joints disable,and lumbar knee pain syndrome in clinical.展开更多
文摘A new pregnane glycoside hypoglaucin G was isolated from Dioscorea collettii var. hypoglauca by bioactivity-guided fractionation, which showed a moderate activity of inducing morphological deformation of the Pyricularia oryzae mycelia. The structure of hypoglaucin G was established as 16 beta-(4'-methyl-5'-O-beta-D-glucopyranosyl-pentanoyloxy)-pregn-5-en-3 beta-ol-20-one 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)-]-beta-D-glucopyranoside by chemical evidence and spectral analysis, especially by 2D-NMR techniques.
文摘Rhizoma Dioscoreae Collettii,is known as Huang Dioscorea collettii,Rhizoma Coptidis Brevisepalae or yellow ginger.It is neutral in nature and bitter in taste which is described to Gan(Liver)meridian,Wei(Stomach)meridian and Pangguang(Bladder)meridian.It was often used in the treatment of chyloid stranguria,gonorrhea,leucorrhagia,rheumatism arthralgia pain,joints disable,and lumbar knee pain syndrome in clinical.
