Diplostomum species metacercariae are trematode parasites that pose serious economic threats to aquaculture practice globally. Identification of Diplostomum at metacercariae stage has remained equivocal, hence lack of...Diplostomum species metacercariae are trematode parasites that pose serious economic threats to aquaculture practice globally. Identification of Diplostomum at metacercariae stage has remained equivocal, hence lack of elucidation of the actual role of these species in fish population. 21 Diplostomoid metacercariae obtained from eyes of Nile tilapia were characterized using 18S and ITS rDNA (ITS1-5.8S-ITS2) genes. Phylogenetic analyses of ITS rDNA gene dataset in the metacercariae revealed close relationship to Diplostomum mashonense and D. baeri. Molecular identification using 18S rDNA sequences revealed close relationship to D. compactum, D. phoxini and D. spathaceum. Overall, genetic analyses in this study depicted a significant unrecognized genetic diversity among Diplostomum species. Successful differentiation of Diplostomum genera in this study using ribosomal markers suggested that 18S and ITS rDNA genes are effective genetic markers for inter-species phylogenetic analysis and should be employed in future for identification of diplostomoidea.展开更多
本实验对倪氏复口吸虫(Diplostomum niedashui Pan et Wang,1963)和湖北复口吸虫(Diplostomum hupehensis Pan et Wang,1963)生活史的各个时期,包括虫卵、毛蚴、母胞蚴、子胞蚴、尾蚴、后囊蚴和成虫,进行了详细的观察。两种复口吸虫的...本实验对倪氏复口吸虫(Diplostomum niedashui Pan et Wang,1963)和湖北复口吸虫(Diplostomum hupehensis Pan et Wang,1963)生活史的各个时期,包括虫卵、毛蚴、母胞蚴、子胞蚴、尾蚴、后囊蚴和成虫,进行了详细的观察。两种复口吸虫的第一中间寄主为斯氏萝卜螺(Radix swinhoei(H.Adams));第二中间寄主为鲤形目的鲤科(Cyprinidae)鱼类,鳅科(Cobitidae)的大鳞副泥鳅(P.dabryanus);鳉形目(Cyprinodontiformes)的食蚊鱼(Gambusia affinis);合鳃目的黄鳝(Monopterus albus);鲈形目的罗非鱼(Tilapia mossambica)等淡水鱼类。终末寄主为红咀鸥(Larus ridibundus ridibundus L.)。展开更多
利用分子生物学方法对采自额尔齐斯河(中国段)的白斑狗鱼(Esox lucius Linnaeus)晶状体内寄生的复口吸虫(Diplostomum)进行了种类鉴定,结果显示其种类为假尾复口吸虫(D.paracaudum),为中国新记录种。通过PCR扩增待鉴定种的部分18S-ITS1-...利用分子生物学方法对采自额尔齐斯河(中国段)的白斑狗鱼(Esox lucius Linnaeus)晶状体内寄生的复口吸虫(Diplostomum)进行了种类鉴定,结果显示其种类为假尾复口吸虫(D.paracaudum),为中国新记录种。通过PCR扩增待鉴定种的部分18S-ITS1-5.8S序列,测序后与Gen Bank中假尾复口吸虫的序列进行比对,发现其相似度达到99.6%。将该序列与7种复口吸虫的ITS1序列进行比对后构建系统进化树,进化树以单殖吸虫Gyrodactylus parvae为外类群,结果显示:待鉴定种与假尾复口吸虫(D.paracaudum)聚为一支,其支持率达到了50%以上,支持它们为同一虫种。展开更多
文摘Diplostomum species metacercariae are trematode parasites that pose serious economic threats to aquaculture practice globally. Identification of Diplostomum at metacercariae stage has remained equivocal, hence lack of elucidation of the actual role of these species in fish population. 21 Diplostomoid metacercariae obtained from eyes of Nile tilapia were characterized using 18S and ITS rDNA (ITS1-5.8S-ITS2) genes. Phylogenetic analyses of ITS rDNA gene dataset in the metacercariae revealed close relationship to Diplostomum mashonense and D. baeri. Molecular identification using 18S rDNA sequences revealed close relationship to D. compactum, D. phoxini and D. spathaceum. Overall, genetic analyses in this study depicted a significant unrecognized genetic diversity among Diplostomum species. Successful differentiation of Diplostomum genera in this study using ribosomal markers suggested that 18S and ITS rDNA genes are effective genetic markers for inter-species phylogenetic analysis and should be employed in future for identification of diplostomoidea.
文摘本实验对倪氏复口吸虫(Diplostomum niedashui Pan et Wang,1963)和湖北复口吸虫(Diplostomum hupehensis Pan et Wang,1963)生活史的各个时期,包括虫卵、毛蚴、母胞蚴、子胞蚴、尾蚴、后囊蚴和成虫,进行了详细的观察。两种复口吸虫的第一中间寄主为斯氏萝卜螺(Radix swinhoei(H.Adams));第二中间寄主为鲤形目的鲤科(Cyprinidae)鱼类,鳅科(Cobitidae)的大鳞副泥鳅(P.dabryanus);鳉形目(Cyprinodontiformes)的食蚊鱼(Gambusia affinis);合鳃目的黄鳝(Monopterus albus);鲈形目的罗非鱼(Tilapia mossambica)等淡水鱼类。终末寄主为红咀鸥(Larus ridibundus ridibundus L.)。
文摘利用分子生物学方法对采自额尔齐斯河(中国段)的白斑狗鱼(Esox lucius Linnaeus)晶状体内寄生的复口吸虫(Diplostomum)进行了种类鉴定,结果显示其种类为假尾复口吸虫(D.paracaudum),为中国新记录种。通过PCR扩增待鉴定种的部分18S-ITS1-5.8S序列,测序后与Gen Bank中假尾复口吸虫的序列进行比对,发现其相似度达到99.6%。将该序列与7种复口吸虫的ITS1序列进行比对后构建系统进化树,进化树以单殖吸虫Gyrodactylus parvae为外类群,结果显示:待鉴定种与假尾复口吸虫(D.paracaudum)聚为一支,其支持率达到了50%以上,支持它们为同一虫种。