Different Re-centrifugation Conditions for Infectious Disease Screening Using the Abbott 12000

We investigated whether an ordinary centrifuge can achieve the standard centrifugal effect required according to specifications for infectious disease screening using the Abbott i2000.Samples were collected and centrifuged following a standard operating procedure(SOP).They were then divided into three groups according to the results of the initial screening tests:a negative group,weak-positive group,and positive group.Twenty negative samples and all weak-positive and positive samples were re-analyzed.Two tubes for each re-analyzed sample were centrifuged simultaneously,one for 10 min at 10000x g,per recommendations,and one for 10 min at 2750^g.No significant difference was found between the groups using different centrifugal forces.There was a strong correlation in the quantitative values between the two conditions of centrifugation.Consistency analysis showed a Cronbach's alpha>0.8 for detection of Treponema pallidum,human immunodeficiency virus(HIV),hepatitis C virus(HCV),and hepatitis B surface antigen in the three groups(negative group,weak-positive group,and positive group)under different centrifugation conditions.Strong consistency was found under different centrifugal conditions,regardless of the initial testing results.In conclusion,we conducted centrifugation steps in duplicate,according to infectious disease screening protocols.Our study showed that all samples should be centrifuged using a recommended relative centrifugal force after a proper clotting time,as in the standard operating procedure of our laboratory.In this way,we were able to obtain the same results using an ordinary centrifuge as those obtained using a high-speed centrifuge,such as the Abbott i2000.

Teaching Physical Assessment and Disease Screening to Students in the Health Professions: A Focus in Pharmacy Education

Background: As pharmacists continue to transition into the provision of public health care, physical assessment and disease screening will become part of their everyday practice, in a similar manner that it has been for doctors and nurses. Objective: The aim was to describe the health assessment of undergraduate curricula across three disciplines involved in public health care and to explore the students’ perceived training in a variety of health assessments. Method: Instructors of courses related to health assessment in medicine, nursing, and pharmacy colleges in Qatar were invited to participate in the curriculum review process. Students’ perceived training in a variety of health assessments was assessed using a self-administered survey. Both descriptive and inferential statistics were used to analyze the survey responses. Results: A narrative description for the similarities and differences in the health assessment curricula across the three colleges was provided. Blood pressure, heart rate, and heart sounds were the health assessments that almost all students received training on, although there were differences in where the training was delivered. The health assessments that the students perceived having received least training on were measuring bone mineral density, blood cholesterol and international normalized ratio reported by only 37%, 41% and 44% of students, respectively. Conclusion: The study identified a close alignment between each of the health discipline’s scope of practice and the health assessment content taught in each individual program. It also identified possible curricular gaps towards learning the skills of patient assessment that may need to be addressed.

Application of Serum Ferritin Combined with Blood Routine Testing in the Screening of Obstetrics and Gynecology Diseases

Objective: To understand the application of serum ferritin combined with blood routine testing in the screening of obstetrics and gynecology diseases in this region. Methods: From January 1, 2017, to October 28, 2020, all obstetric pregnant women, inpatient maternal, gynecological outpatient, and gynecological inpatients in our hospital’s outpatient and inpatient clinics were collected for serum ferritin determination and blood routine related index detection and analysis Changes in results. The application value of serum special protein in gynecological and obstetrical disease screening was put forward. Results: A total of 15,656 cases of obstetrics and gynecology patients were collected, of which 15,300 were pregnant women. Comparing maternity checkups with hospitalized puerpera, the difference of all indexes except RBC was statistically significant (P < 0.05);Comparison of various indicators between gynecological outpatient clinics and gynecological hospitalizations, maternity checkups and gynecological outpatient clinics, inpatient maternal and gynecological hospitalizations, etc.;there was no statistically significant difference in all indicators (P > 0.05). Comparison of hospitalized puerpera and gynecological hospitalization, the difference of other indexes except for MCV, MCH was statistically significant (P < 0.05);Comparing hospitalized puerpera and gynecological clinics, the difference of HGB results was statistically significant (P < 0.05), but the difference of other indexes was not statistically significant (P > 0.05). Conclusion: During the maternity check-up period, the ferritin consumption is too large, the blood volume increases, and the blood routine-related items are reduced. The consumption is greater for a period of time after delivery, and recovery requires a certain process, which is relatively low;The ferritin in the body slowly recovered and the ferritin concentration increased. This process is a process of physiological change and does not involve related diseases, but from this result, we are also required to strengthen nutrition for pregnant women in order to give birth to healthier babies. The ferritin test of gynecological patients is mainly a screening of related diseases, and the results of this time did not screen out serious diseases.

Bioinformatics Analysis Raises Candidate Genes in Blood for Early Screening of Parkinson's Disease

Parkinson＇s disease （PD） is a typical degenerative disease, which is characterized by the most obvious symptoms of movement dysfunction, including shaking, rigidity, slowness of movement and difficulty in walking and gait. This disease can not be clearly identified through laboratory tests at present, thus application of high-throughput technique in studying the expression profiles of PD helps to find the genetic markers for its early diagnosis. Studies on expression profiles of neurodegenerative diseases have revealed the novel genes and pathways involved in the progress of illness. In this study, the expression profiles of PD in blood were compared, showing that 181 differentially expressed genes （DEG） exhibit a similar expression trend both in patients and in normal controls.

基于知识图谱的疾病筛查模型研究:以新型冠状病毒肺炎为例

目的形成一套基于机器学习等技术、面向疾病筛查的模型构建方法与路径,推进我国常见疾病、传染病的预测预防与早期干预。方法以新型冠状病毒肺炎为例,基于统计学和知识图谱理论,利用大数据和机器学习技术,提取疾病数据集的特征数据,构建基于机器学习的疾病早期筛查模型。结果对500例风险人员进行模型测试,并依据核酸检测结果评价模型的准确率,测试结果表明,模型有较高的准确率。结论研究构建的疾病筛查模型较好地解决了传统疾病筛查效率低、准确性差的瓶颈问题,实现了居民及医疗机构对疾病的早期筛查与识别,可以在不同医疗机构以及互联网端推广使用。

Screening for coronary artery disease in asymptomatic patients with type 2 diabetes mellitus

In China, the prevalence of type 2 diabetes mellitus is .increasing rapidly due to aging of the population,increased frequency of obesity, and suboptimal nutritional habits. Currently, the number of people with diabetes was estimated to be 20 million and will be projected to double by the year 2030. The age-adjusted prevalence of diabetes among patients with acute myocardial infarction has increased significantly over the past two decades, and diabetic patients without known coronary artery disease have similar rates of subsequent myocardial infarction compared with non-diabetic counterparts with a previous myocardial infarction.4 Diabetes is a strong risk factor for cardiovascular disorders,

The Effects of Absorbable Materials in the Treatment for Non-Weight-Bearing Bone Fractures of Extremities

With the application of queuing theory model,this paper regards breast cancer screening in primary health care service center as a queuing system.With the help of the tide of sharing economy,it puts forward a joint screening scheme based on M/M/s model under shared mode,and compares it with M/M/1 model of non-shared mode,shortens patient waiting time by sharing medical resources,plans existing resources reasonably,and enhances the comprehensive strength of primary health care system so as to optimize the screening of breast cancer at the grass-roots level.

A New Next-Generation Sequencing-Based Assay for Concurrent Preimplantation Genetic Diagnosis of Charcot-Marie-Tooth Disease Type 1A and Aneuploidy Screening

Charcot-Marie-Tooth （CMT） disease is the most common hereditary neuropathy, with a population prevalence of 1 in 2500. CMT disease type 1A （CMT1A）, accounting for ~70% of CMT1 cases and ~ 50% of all CMT cases, is transmitted in an autosomal dominant manner. CMT1A maps to chromo- some 17pl 1.2 and is caused, in the majority of cases, by a 1.4- Mb tandem duplication that includes the peripheral myelin protein22 （PMP22） gene （Li et al., 2013）. The disease usually presents in the first 20 years of age, causing difficulty in walking or running, distal symmetrical muscle weakness and wasting, and sensory loss （van Paassen et al., 2014）.

Yeast Two-Hybrid Screening for Proteins that Interact with the Extracellular Domain of Amyloid Precursor Protein

Alzheimer＇s disease（AD） is a neurodegenerative disorder in which amyloid b plaques are a pathological characteristic. Little is known about the physiological functions of amyloid b precursor protein（APP）. Based on its structure as a type I transmembrane protein, it has been proposed that APP might be a receptor, but so far, no ligand has been reported. In the present study, 9 proteins binding to the extracellular domain of APP were identified using a yeast two-hybrid system. After confirming the interactions in the mammalian system, mutated PLP1,members of the FLRT protein family, and KCTD16 were shown to interact with APP. These proteins have been reported to be involved in Pelizaeus-Merzbacher disease（PMD） and axon guidance. Therefore, our results shed light on the mechanisms of physiological function of APP in AD, PMD, and axon guidance.

1,5-O-Dicaffeoyl-quinic Acid as a Novel Potential NMDA Receptor Inhibitor from Traditional Chinese Medicine Database by Virtual Screening

Objective Neurodegenerative diseases, such as ischemia, traumatic injury, Alzheimer＇s disease, and Parkinson＇s disease are characterized by neuronal loss and dysfunction. It is known that glutamate-induced toxicity plays an important role in neurodegenerative diseases. Glutamate toxicity seems to be mediated by excessive influx of Ca^（2＋） into neuronal cells through activation of N-methyl-D-aspartate（NMDA） receptor. To search for potential NMDA receptor inhibitors in traditional Chinese medicine. Methods A series of computer methods including drug-likeness evaluation, ADMET tests as well as molecular docking have been used. Results 1,5-O-dicaffeoyl-quinic acid was identified as NMDA receptor inhibitor by virtual screening. Its neuroprotective activity was further confirmed by in vitro test. 1,5-O-dicaffeoyl-quinic acid showed strong neuroprotection against NMDA-induced cell injury. Conclusion 1,5-O-Dicaffeoylquinic acid may be regarded as a potential NMDA receptor inhibitor for the prevention and treatment of neurodegenerative disorders.