Research Progress of Radical Treatment of Helicobacter Pylori Based on Drug Sensitivity Test as First-Line Treatment

Helicobacter pylori (HP) infection is a global problem that affects about half of the world’s population and requires sufficient attention in clinical and scientific work. Due to differences in economic and medical conditions among countries around the world, there is currently no unified treatment plan for anti-HP. In China, empirical quadruple therapy is mainly used. With the abuse of antibiotics, many patients face the problem of secondary eradication after failure, and the resistance rate of HP is gradually increasing. After eradication failure, drug sensitivity cultivation is carried out to choose sensitive antibiotics for treatment. A new strategy is currently needed to address how to improve the eradication rate of HP during the first eradication. This article aims to discuss the first-line treatment plans and research progress for eradicating HP based on drug sensitivity testing before eradication. Compared with traditional empirical therapies, treatment based on drug sensitivity results can effectively improve the eradication rate of HP, and reduce drug resistance rates, and adverse reactions, among other benefits. .

Improving treatment plan and mental health in children with abdominal infection for broad-spectrum bacterial infections

BACKGROUND Pediatric abdominal infection is a common but serious disease that requires timely and effective treatment.In surgical treatment,accurate diagnosis and rational application of antibiotics are the keys to improving treatment effects.AIM To investigate the effect of broad-spectrum bacterial detection on postoperative antibiotic therapy.METHODS A total of 100 children with abdominal infection who received surgical treatment in our hospital from September 2020 to July 2021 were grouped.The observation group collected blood samples upon admission and sent them for broad-spectrum bacterial infection nucleic acid testing,and collected pus or exudate during the operation for bacterial culture and drug sensitivity testing;the control group only sent bacterial culture and drug sensitivity testing during the operation.RESULTS White blood cell count,C-reactive protein,procalcitonin,3 days after surgery,showed better postoperative index than the control group(P<0.05).The hospital stay in the observation group was significantly shorter than that in the control group.The hospitalization cost in the observation group was significantly lower than that in the control group,and the difference between the two groups was statistically significant(P<0.05).CONCLUSION Early detection of broad-spectrum bacterial infection nucleic acids in pediatric abdominal infections can help identify pathogens sooner and guide the appropriate use of antibiotics,improving treatment outcomes and reducing medical costs to some extent.

Analysis of Chinese and American cultural values in the film——《Gua Sha Treatment》

The film——《Gua Sha Treatment》 tells the story of a Chinese family who immigrates to the Unit ed States,but falls into a series of troubles because the traditional Chinese medical therapy-Gua Sha is not understood and recognized by Americans.The film takes Gua Sha as the main line,reflecting the misunderstanding,conflict and integration caused by different cultural values be-tween China and America.This paper interprets the differences and conflicts between Chinese and American cultural values contained in the film from the perspectives of ethics,face and friendship,and points out that mutual adaptation and tolerance are effective meth-ods to overcome cultural barriers and improve the quality of communication in cross-cultural communication.

Establishment of a Highly Efficient Regeneration System for the Mature Embryo Culture of Wheat

Establishment of a highly efficient regeneration system for the mature embryo of wheat will provide a convenient tool for wheat tissue culture and transformation, thereby facilitating the transformation of foreign genes into wheat. By using the mature embryos derived from 20 different wheat lines including Shi 4185, Yumai 66, Lunxuan 987, CB037, Yangmai 6, Xinchun 9, Bobwhite, Han 6172, Zheng 9023, Jimai 20, Ningchun 4, and Jing 411, the effects of some factors including inoculation methods, initiating culture media, organic additives, antioxidants, and auxins on the regeneration from the explants were evaluated. The results indicated that the scraping embryo culture was better than the whole embryo culture, the Aa medium was better than the SD2 medium and dicamba was better than 2,4-D in increasing the regeneration frequency. An Adi medium was established in this study by adding silver nitrate, cysteine, ascorbic acid, dicamba, glutamine into the Aa medium at the concentration of 4,40, 100, 2, and 5 mg L^-1, respectively. By using the Adi medium and the scraping technique, the regeneration frequencies of the mature embryos of CB037, Lunxuan 987, Hart 6172, Yangmai 6, Bobwhite, Zheng 9023, Shi 4 185, and Jimai 20 became 85.6, 60,1, 46.0, 42.1,42.0, 34.0, 33.0, and 32.0%, respectively, which were about 5-8 times higher than that obtained from the conventional culture mediums and techniques. This novel regeneration system could be helpful in wheat transformation.

Do not lose the moon while counting the stars:Conventional IVF versus add-on treatments

When we look back at-more than four decades-the history of assisted reproductive treatments(ART),a continuous effort to optimise laboratory conditions and to improve treatment protocols can be seen.In basic terms,the aim of research about culture media and laboratory environments is to create as more physiological condition as possible,which means reducing the gap between the not-fully-known in vivo conditions and their in vitro counterparts.

论历代治黄方略中的“中国智慧”

回顾历代疏水导水、防水御水、束水攻沙、谐水让水、调水调沙、协同治理和综合创新等黄河治理方略,总结概括其深邃的时代价值,提炼彰显其启迪现在及将来的“中国智慧”,包括“疏导”与“堵塞”辩证实施是中国治水成功的基本理路、“利人”与“利水”双向互动是中国治水成功的根本原因、“治水”与“治人”两手发力是中国治水成功的重要法宝、“治水”与“治国”融通联动是中国治水成功的切实保障等,对于进一步增强文化自信,彰显中华先进水文化成果,建设中华民族现代文明,具有重要的理论价值和现实意义。

青海茄参种子的萌发特性

【目的】本试验旨在探索青海茄参种子的萌发特性。【方法】以青海茄参(Mandragora chinghaiensis)的种子为试验材料,选择常规播种,低温处理(4℃),组织培养3种方式进行萌发试验。【结果】青海茄参种子经TTC法染色检测,活力为65%;常规播种与低温处理无法使青海茄参种子萌发,只有组织培养的方式获得了种苗;对种子的预处理最适宜的方式为300 mg/L的GA3浸泡16 h;最佳的消毒方式为20%NaClO消毒15 min;最适宜萌发的基本培养基为1/2MS;最有利于青海茄参种子萌发的激素组合为1/2 MS+6-BA 2 mg/L+NAA 0.1 mg/L+GA30.3 mg/L。【结论】探索青海茄参种子的萌发体系,可以缓解青海茄参人工繁殖困难种苗极难获得的瓶颈问题,为进一步研究其药学价值及生物学特性提供新的支持。

香云纱真丝织物抗紫外线性能研究进展

香云纱作为国家级非物质文化遗产,是世界纺织品中唯一用纯植物染料染色的丝绸面料,具有天然环保、挺括顺滑、抗菌除臭、防晒防水等优势,被誉为“软黄金”。现阶段由于臭氧层的严重破坏,对人类健康及其他方面产生重要威胁,织物抗紫外线性能研究逐渐成为纺织品行业的重要方向。相比于普通真丝织物,香云纱由于特殊染整工艺,具有优异的紫外线防护性能。但该染整过程较为传统,实际生产具有较大限制,行业产业化率低。基于此文章综述了香云纱特殊染整工艺及其抗紫外线性能、其他真丝织物抗紫外线研究等内容,总结了香云纱抗紫外线染整过程存在的问题与改进方向,并对其发展趋势进行了展望,以指导实际生产应用,为未来香云纱产品设计创新及性能改善提供借鉴,提升产品服用性能,推动行业发展。

榆叶梅高效成熟胚培养成苗体系的建立

以榆叶梅(Prunus triloba Lindl.)成熟胚为外植体,构建榆叶梅稳定高效的胚培养成苗体系,测定萌发率、平均株高、生根率、平均数、平均根长、愈伤率和叶片形态等指标,设置MS、1/2 MS、WPM等3个基础培养基,筛选榆叶梅胚培养成苗的最适培养基;利用6-BA、NAA、IBA等3种植物生长调节剂和活性炭进行胚的萌发和生根试验,其中IBA、NAA均设0.05、0.1、0.2 mg/L等3个水平,6-BA设1、2 mg/L两个水平,统计胚萌发和生根情况,筛选榆叶梅胚培养成苗体系最佳培养基类型;在最适合榆叶梅胚培养生长培养基基础上,再设置不同低温处理时间(0、20、40、60、80 d)进行愈伤组织诱导及分化实验,统计愈伤率和叶片形态情况;最后分析不同子叶保留程度(完整子叶、1/2子叶和1/4子叶)对胚培养萌发和生根的影响。结果表明,MS培养基为榆叶梅胚的萌发和上胚轴生长的最适培养基;综合各项试验的最佳影响因子,榆叶梅在MS+6-BA 1.0 mg/L+IBA 0.1 mg/L+NAA 0.1 mg/L+活性炭500 mg/L培养基上萌发率和生根率最高,分别为83.33%、86.67%;4℃冷藏60~80 d为最适宜榆叶梅胚培养发育和正常生长的时间,冷藏时间80 d时萌发率达100%,生根率为93.33%,叶片绿色平展;保留完整子叶有利于促进上胚轴的伸长,保留1/4子叶有利于促进下胚轴的伸长。本研究筛选出最适宜榆叶梅胚的培养成苗培养基为MS+6-BA 1.0 mg/L+IBA 0.1 mg/L+NAA 0.1 mg/L+活性炭500 mg/L。

药食两用植物黑老虎组培快繁体系研究

为改变药食同源植物黑老虎野生资源日趋减少、难以满足市场需求的现状,对组培快繁体系中的培养基配制及组培瓶苗培育条件进行了研究,以期建立黑老虎组培快繁体系,为规模化种苗繁殖奠定基础。结果表明,以MS+2.0 mg/L 6-BA+0.2 mg/L NAA为初代培养基,以B5+2.0 mg/L 6-BA+0.2 mg/L NAA为增殖培养基,以1/2 MS+0.1 mg/L 6-BA+1.5 mg/L NAA+0.3%活性炭为生根培养基,先暗培养,再光照培养,生根率可达100%,且小苗主根粗,长势强。组培苗最佳炼苗时间为15 d,最佳移栽基质为塘泥∶蔗渣∶鸡粪∶过磷酸钙=3.9∶4∶2∶0.1,最佳遮阳模式为半遮阳,最佳培育期为60 d。

多学科协作诊疗模式在临床医学专业整体思维培养中的效果

目的研究多学科协作诊疗(multi-disciplinary collaborative diagnosis and treatment,MDT)模式在临床医学专业整体思维培养中的效果。方法选取雅安职业技术学院临床医学院2024年3—6月的33名临床专业22级学生作为观察组,另选取雅安职业技术学院临床医学院2023年3—6月的33名临床专业21级学生作为对照组。观察组采取MDT模式进行教学,对照组采用传统教学。比较2组学生的考试成绩、整体思维能力、考核评价得分以及教学总满意度。结果观察组学生考试成绩中的临床多学科基础知识[(22.42±1.28)分]、临床专业知识[(22.05±1.96)分]、临床技术实操[(18.75±1.57)分]、临床试验设计和验证[(13.29±0.42)分]及医患沟通基本技能[(14.66±0.25)分]成绩均高于对照组[(18.02±2.38)分、(15.33±3.01)分、(13.28±2.64)分、(10.04±2.02)分、(11.50±1.12)分],差异有统计学意义(P<0.05)。观察组学生整体思维能力中的临床资料检索能力[(18.33±1.15)分]、临床实践操作能力[(17.18±2.86)分]、临床沟通应变能力[(18.19±0.97)分]、临床分析理解信息能力[(19.13±0.32)分]、临床团队协作能力[(18.33±1.67)分]及临床解决问题与创新能力[(18.43±1.07)分]评分均高于对照组[(12.06±2.11)分、(12.77±2.71)分、(13.28±2.24)分、(13.34±1.75)分、(12.34±1.92)分、(13.28±2.68)分],差异有统计学意义(P<0.05)。观察组学生考核评价中的课前任务[(95.11±4.35)分]、课堂参与度[(96.11±2.34)分]、方案可行度[(92.49±4.22)分]、操作规范[(93.17±2.33)分]、医患沟通[(93.75±3.58)分]、人文关怀[(95.77±3.28)分]、课后作业评价得分[(95.65±4.30)分]均高于对照组[(80.23±5.35)分、(83.38±3.44)分、(80.28±3.17)分、(83.65±2.78)分、(80.37±3.65)分、(84.38±4.49)分、(80.44±5.75)分],差异有统计学意义(P<0.05)。观察组的教学总满意度为100%,高于对照组的69.70%,差异有统计学意义(P<0.05)。结论在临床医学专业教学中采取MDT模式能有效提高医学生的考试成绩,培养学生的整体思维能力,提高教学总满意度,具有显著的教学效果。

基于官修方书探讨宋代香佩疗法特点

宋代为方剂学和香文化皆蓬勃发展的重要历史时期,香佩疗法在当时富有鲜明的时代特色。梳理宋代三大官修方书中的香佩方剂发现,宋时香佩疗法用药多样,除了常用矿物药和骨类药之外,本土香药与外来香药皆有大量使用;用法灵活,有膏、丹、丸、散等多种剂型,可佩戴于头、手、颈、胸等身体各部位,但总体以丸剂佩于上肢者为多;用途广泛,以辟疫驱邪治疗传染性疾病为主,内外妇儿各科杂症也有涉及,为后世香佩疗法的发展打下了良好的基础。

中医药院校廉洁文化教育研究——以浙江中医药大学为例

[目的]探索中医药院校廉洁文化教育新路径,从中医药文化中提取廉洁元素,专注打造中医药院校的廉洁教育方案。[方法]通过文献研究法,提炼中医药文化与廉洁文化的相通性,运用“四诊合参”“辨证论治”“良药金方”“标本兼治”等中医理念,构建中医药院校廉洁文化教育研究框架。[结果]中医药文化蕴含丰富的廉洁文化精神内涵和方法论,两者具有高度契合性。中医药院校廉洁文化教育存在的“四诊合参”缺位、“辨证论治”乏力、“良药金方”少有、“标本兼治”不牢等问题,要通过摸清廉洁思想教育实际、完善廉洁文化教育制度、巧妙开展廉洁文化教育、筑牢廉洁文化教育防线等举措,进一步提升中医药院校廉洁文化教育成果。[结论]通过对浙江中医药大学廉洁文化教育的个案研究,摸清廉洁思想教育实际,完善廉洁文化教育制度,巧妙开展廉洁文化教育,筑牢廉洁文化教育防线,为高校开展廉洁文化教育提供示范。

福建省鳗鲡养殖尾水治理模式分析与推进建议

随着中国生态文明建设步伐的加快,渔业进入了高质量发展的新时代。鳗鲡作为福建省淡水养殖的主要品种之一,其养殖尾水排放问题日益突出。目前福建省鳗鲡养殖尾水治理的模式主要有传统化学处理、改进化学处理、生态综合种养治理、双循环零排放养鳗模式等,本文阐述了这四种模式的主要技术工艺、特点,分析了其主要优劣势,并对今后鳗鲡养殖尾水的治理工作提出了推进建议,为福建省乃至中国水产养殖业尾水治理技术的提升改进提供了良好借鉴。

Comparison of Histological, Microbiological, and Molecular Methods in Diagnosis of Patients with TBLN Having Different Anti-TB Treatment Background

Objective The influence of anti-tuberculosis （TB） treatment history on tuberculous lymphadenitis （TBLN） diagnosis is unclear. Therefore, this study aims to evaluate the diagnostic methods, including histology, microbiology, and molecular tests, used for TBLN. Methods In this study, suspected patients with TBLN and having different anti-T8 treatment background were enrolled. All the samples were tested simultaneously by histology, ZiehI-Neelsen （ZN） staining, mycobacterial culture （culture）, Xpert MTB/RIF （xpert）, real-time PCR, and high-resolution melting curve PCR （HRM）. Thereafter, the performance of these methods on samples with different anti-TB treatment background was assessed. Results In our study, 89 patients were prospectively included 82 patients with TBLN and 7 with other diseases. The overall sensitivities of Xpert, real-time PCR, histology, ZN staining, and culture were 86.6%, 69.5%, 58.5%, 43.9%, and 22.0%, respectively. The anti-TB treatment history revealed dramatic influences on the sensitivity of culture （P 〈 0.0001）. In fact, the treatment that lasted over 3 months also influenced the sensitivity of Xpert （P 〈 0.05）. However, the treatment history did not affect the performance of remaining tests （P 〉 0.05）. For rifampicin drug susceptibility test （DST）, the anti-T8 treatment showed only significant influence on the success rate of culture DST （P = 0.001）, but not on those of Xpert and HRM tests （P 〉 0.05）. Conclusion Other tests as welt as culture should be considered for patients with TBLN having retreatment history or over 1-month treatment to avoid false negative results.

Curcumin pretreatment and post-treatment both improve the antioxidative ability of neurons with oxygen-glucose deprivation

Recent studies have shown that induced expression of endogenous antioxidative enzymes thr- ough activation of the antioxidant response element/nuclear factor erythroid 2-related factor 2 （Nrf2） pathway may be a neuroprotective strategy. In this study, rat cerebral cortical neurons cultured in vitro were pretreated with 10 ktM curcumin or post-treated with 5 pM curcumin, respectively before or after being subjected to oxygen-glucose deprivation and reoxygenation for 24 hours. Both pretreatment and post-treatment resulted in a significant decrease of cell injury as indicated by propidium iodide/Hoechst 33258 staining, a prominent increase of Nrf2 protein expression as indicated by western blot analysis, and a remarkable increase of protein expression and enzyme activity in whole cell lysates of thioredoxin before ischemia, after ischemia, and after reoxygenation. In addition, post-treatment with curcumin inhibited early DNA/RNA oxidation as indicated by immunocytochemistry and increased nuclear Nrf2 protein by inducing nuclear accumulation of Nrf2. These findings suggest that curcumin activates the expression of thi- oredoxin, an antioxidant protein in the Nrf2 pathway, and protects neurons from death caused by oxygen-glucose deprivation in an in vitro model of ischemia/reperfusion. We speculate that pharmacologic stimulation of antioxidant gene expression may be a promising approach to neu- roprotection after cerebral ischemia.

一株白地霉在低碳氮比废水中降氨氮新功能的研究

该文从垃圾渗滤液中筛选出一株低C/N营养条件下氨氮去除效果显著的菌株。ITS序列测序鉴定表明,该菌株为白地霉(Galactomyces candidum)。经不同pH、温度、C/N的培养条件下培养24 h,测定其生长密度及氨氮去除情况,结果发现,白地霉培养基最佳降氨氮条件为:pH 8.0,C/N 1.5,温度30℃,其最佳氨氮去除率可达93.1%。该文发现了白地霉在污水处理,尤其是低C/N污水处理中具有氨氮去除的新功能,为其在低C/N污水生化处理工业化应用提供了新的菌株资源和技术途径。

试管苗热处理结合茎尖培养脱除苹果矮化自根砧M9-T337、B9、GM256、M26病毒试验

【目的】苹果矮化自根砧脱毒苗木的推广应用,对推动苹果栽培制度的变革和果业增效、果农增收都具有重要的意义。【方法】对苹果矮化自根砧M9-T337、B9、GM256、M26茎尖和试管苗采用茎尖培养结合试管苗热处理脱除病毒方法,开展了脱毒试验。【结果】经检测,4个苹果砧木苹果褪绿叶斑病毒(ACLSV)茎尖脱毒率为7.1%,苹果茎尖病毒(ASGV)脱毒率为3.6%;二次茎尖热处理后,4个苹果砧木苹果褪绿叶斑病毒(ACLSV)脱毒率为51.0%,达到了预期的目标,对苹果矮化自根砧脱毒苗木的生产起到了促进作用。【结论】对脱毒后的材料送检,4个样品中苹果矮化自根砧M9-T337、TM26不携带苹果茎痘病毒(ASPV)、苹果茎沟病毒(ASGV)、苹果褪绿叶病毒(ACLSV)、苹果花叶病毒(APMV)、苹果诱果类病毒(ASSVd),病毒的脱毒率达到100%;B9携带苹果茎痘病毒(ASPV),GM256携带苹果褪绿叶斑病毒(ACLSV),这两种砧木病毒的脱毒率达到80%,脱毒效果较好,该方法可在苗木繁育中推广应用,以促进苹果矮化自根砧脱毒苗木的生产。