AIM: To investigate relationships between colorectal adenoma incidence, metabolic syndrome (MS) components and lifestyle factors. METHODS: We conducted a retrospective cohort study using data from individuals who had ...AIM: To investigate relationships between colorectal adenoma incidence, metabolic syndrome (MS) components and lifestyle factors. METHODS: We conducted a retrospective cohort study using data from individuals who had multiple sigmoidoscopies for colon cancer at the Health Promotion Center of Ulsan University Hospital in Korea from 1998 to 2007. RESULTS: By multivariate analysis, the incidence of distal colon adenoma was increased by more than 1.76 times in individuals with at least one component of MS compared to those without a component of MS. After adjustment for age, gender, smoking, drinking, and physical exercise, only high body mass index (BMI) was significantly associated with the incidence of distal colon adenoma (Hazard ratio 1.66, 95% confidence interval 1.05-2.62). CONCLUSION: Our results suggest that high BMI may increase the risk of colorectal adenoma in Korean adults.展开更多
AIM: To study the origin of calcium necessary for agonist-induced contraction of the distal colon in rats.METHODS: The change in intracellular calcium concentration ([Ca2+]i) evoked by elevating external Ca2+ was dete...AIM: To study the origin of calcium necessary for agonist-induced contraction of the distal colon in rats.METHODS: The change in intracellular calcium concentration ([Ca2+]i) evoked by elevating external Ca2+ was detected by fura 2/AM fluorescence. Contractile activity was measured with a force displacement transducer. Tension was continuously monitored and recorded using a Powerlab 4/25T data acquisition system with an ML110 bridge bioelectric physiographic amplifier.RESULTS: Store depletion induced Ca2+ influx had an effect on [Ca2+]i. In nominally Ca2+-free medium, the sarco-endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin (1 μmol/L) increased [Ca2+]i from 68 to 241 nmol/L, and to 458 (P < 0.01) and 1006 nmol/L (P < 0.01), respectively, when 1.5 mmol/L and 3.0 mmol/L extracellular Ca2+ was reintroduced. Furthermore, the change in [Ca2+]i was observed with verapamil (5 μmol/L), La3+ (1 mmol/L) or KCl (40 mmol/L) in the bathing solution. These channels were sensitive to La3+ (P < 0.01), insensitive to verapamil, and voltage independent. In isolated distal colons we found that in normal Krebs solution, contraction induced by acetylcholine (ACh) was partially inhibited by verapamil, and the inhibitory rate was 41% (P < 0.05). On the other hand, in Ca2+-free Krebs solution, ACh induced transient contraction due to Ca2+ release from the intracellular stores. The transient contraction lasted until the Ca2+ store was depleted. Restoration of extracellular Ca2+ in the presence of atropine produced contraction, mainly due to Ca2+ influx. Such contraction was not inhibited by verapamil, but was decreased by La3+ (50 μmol/L) from 0.96 to 0.72 g (P < 0.01). CONCLUSION: The predominant source of activator Ca2+ for the contractile response to agonist is extracellular Ca2+, and intracellular Ca2+ has little role to play in mediating excitation-contraction coupling by agonists in rat distal colon smooth muscle in vitro. The influx of extracellular Ca2+ is mainly mediated through voltage-, receptor- and store-operated Ca2+ channels, which can be used as an alternative to develop new drugs targeted on the dysfunction of digestive tract motility.展开更多
AIM:To investigate whether Na+-K+-2Cl-cotransporter(NKCC2)is expressed in the mouse distal colonic epithelia and whether it is regulated by vasopressin in the colon.METHODS:The mRNA expression of NKCC2 in the mouse co...AIM:To investigate whether Na+-K+-2Cl-cotransporter(NKCC2)is expressed in the mouse distal colonic epithelia and whether it is regulated by vasopressin in the colon.METHODS:The mRNA expression of NKCC2 in the mouse colonic mucosa was examined by reverse transcription-polymerase chain reaction.NKCC trafficking in the colon stimulated by 1-D-amino(8-D-arginine)-vasopressin(dDAVP)infusion(10 ng/mouse,intraperitoneal injection)within 15 min,30 min and 1h was investigated by laser confocal scanning microscopy.Total and membrane NKCC2 expression in the colonic mucosa from control and dDAVP-treated mice was detected by Western blotting.Short circuit current method was performed to determine regulation of NKCC2 by vasopressin in the colon.RESULTS:NKCC2 was predominantly located in the apical region of the surface of the distal colonic epithelia;by comparison,a large amount of NKCC1 was distributed in the basolateral membrane of the lower crypt epithelia of the mouse distal colon.Short-term treatment with dDAVP,a V2-type receptor-specific vasopressin analog,induced NKCC2 re-distribution,i.e.,NKCC2traffics to the apical membrane after dDAVP stimulation.In contrast,no obvious NKCC1 membrane translocation was observed.Western blotting results confirmed that membrane NKCC2 had significantly higher abundance in the dDAVP-treated mouse colonic mucosa relative to that in the untreated control,which is consistent with our immunostaining data.Moreover,the short-circuit current method combined with a NKCC2 inhibitor demonstrated that NKCC2 was also activated by serosal vasopressin in isolated distal colonic mucosa.CONCLUSION:Our results provide direct evidence that vasopressin also plays an important role in the colonic epithelia by stimulating NKCC2 trafficking to the apical membrane and inducing NKCC2-mediated ion transport.展开更多
AIM: To investigate the effects of luminal exposure to H2O2 and two related thiol oxidizing agents on basal and stimulated chloride secretion in native colon using electro- physiological and pharmacological approaches...AIM: To investigate the effects of luminal exposure to H2O2 and two related thiol oxidizing agents on basal and stimulated chloride secretion in native colon using electro- physiological and pharmacological approaches. METHODS: Unstripped rat distal colon segments were mounted in Ussing chambers. Potential difference, cal- culated resistance and short-circuit current across un- stripped colon segments were monitored with a dual voltage/current clamp. Paracellular permeability was assessed by measuring the mucosa-to-serosa ? ux of a ? uorescent probe (FITC). RESULTS: Luminal exposure to hydrogen peroxide tran- sitorily stimulated chloride secretion without altering bar- rier function. This stimulatory effect could be blocked by basolateral atropine but not indomethacin. The cysteine and methionine oxidizing compounds, phenylarsine oxide and chloramine T respectively, mimicked the effect of H2O2, except for a drop in transcolonic resistance after 30 min. In contrast to the observed stimulatory effect on basal secretion, cAMP-stimulated electrogenic ion trans- port was blunted by luminal H2O2. However, the Ca2+- activated response remained unchanged. CONCLUSION: H2O2 may be an important selective modulator of intestinal ion and water secretion in certain pathologic conditions such as in? ammation or ischemia- reperfusion by multiple mechanisms.展开更多
目的 探讨中药灌肠联合温针灸治疗远端结肠型溃疡性结肠炎的疗效。方法 将90例远端结肠型溃疡性结肠炎患者分为3组。在常规西药基础上,中药灌肠组予以中药灌肠,温针灸组予以温针灸治疗,联合治疗组则予以中药灌肠联合温针灸治疗。比较3...目的 探讨中药灌肠联合温针灸治疗远端结肠型溃疡性结肠炎的疗效。方法 将90例远端结肠型溃疡性结肠炎患者分为3组。在常规西药基础上,中药灌肠组予以中药灌肠,温针灸组予以温针灸治疗,联合治疗组则予以中药灌肠联合温针灸治疗。比较3组患者的临床疗效、生化指标及炎症因子水平、中医症状评分、结肠镜评分、结肠组织病理评分、黏膜愈合及复发情况、不良反应。结果 联合治疗组临床治疗总有效率显著高于中药灌肠组及温针灸组( P <0.05);联合治疗组治疗后白细胞计数(WBC)、血小板(PLT)、肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)水平均显著低于中药灌肠组及温针灸组( P <0.05);联合治疗组治疗后中医症状评分、结肠镜评分、结肠组织病理评分均显著低于中药灌肠组及温针灸组( P < 0.05);联合治疗组黏膜愈合率、复发率均显著优于中药灌肠组及温针灸组( P <0.05);3组不良反应发生率差异无统计学意义( P >0.05)。结论 中药灌肠联合温针灸治疗远端结肠型慢性溃疡性结肠炎的疗效显著,可显著改善中医症状,降低生化指标与炎症因子水平。展开更多
目的研究多巴胺(DA)对大鼠结肠运动影响的机制。方法采用离体组织灌流方法记录大鼠远端结肠自发性节律运动,观察DA的作用以及阻断剂的影响,再用反转录实时多聚酶链反应(real time RT-PCR)检测受体基因的表达。结果DA(≥1.0×10-5mol...目的研究多巴胺(DA)对大鼠结肠运动影响的机制。方法采用离体组织灌流方法记录大鼠远端结肠自发性节律运动,观察DA的作用以及阻断剂的影响,再用反转录实时多聚酶链反应(real time RT-PCR)检测受体基因的表达。结果DA(≥1.0×10-5mol/L)对结肠远端(紧接肛门淋巴结近端)离体纵行肌条(2.0 mm×10 mm)的运动具有抑制作用,多巴胺受体阻断剂(D1受体阻断剂SCH23390,1.0×10-7mol/L,D2受体阻断剂Sulpide,1.0×10-7mol/L)不能阻断多巴胺的抑制效应,但加入β3受体抑制剂cyanopindolol(7.5×10-7mol/L),DA的抑制作用显著减弱。real time RT-PCR检测发现β1、β2、β3受体mRNA在远端结肠均有表达。结论DA可通过β3受体发挥对远端结肠运动的抑制作用。展开更多
基金Supported by The Biomedical Research Center Promotion Fund of the Ulsan University Hospital (UUH-2008-08)
文摘AIM: To investigate relationships between colorectal adenoma incidence, metabolic syndrome (MS) components and lifestyle factors. METHODS: We conducted a retrospective cohort study using data from individuals who had multiple sigmoidoscopies for colon cancer at the Health Promotion Center of Ulsan University Hospital in Korea from 1998 to 2007. RESULTS: By multivariate analysis, the incidence of distal colon adenoma was increased by more than 1.76 times in individuals with at least one component of MS compared to those without a component of MS. After adjustment for age, gender, smoking, drinking, and physical exercise, only high body mass index (BMI) was significantly associated with the incidence of distal colon adenoma (Hazard ratio 1.66, 95% confidence interval 1.05-2.62). CONCLUSION: Our results suggest that high BMI may increase the risk of colorectal adenoma in Korean adults.
基金Supported by Grants from the fund of Wannan Medical College Scientific Research, No. WK 200729
文摘AIM: To study the origin of calcium necessary for agonist-induced contraction of the distal colon in rats.METHODS: The change in intracellular calcium concentration ([Ca2+]i) evoked by elevating external Ca2+ was detected by fura 2/AM fluorescence. Contractile activity was measured with a force displacement transducer. Tension was continuously monitored and recorded using a Powerlab 4/25T data acquisition system with an ML110 bridge bioelectric physiographic amplifier.RESULTS: Store depletion induced Ca2+ influx had an effect on [Ca2+]i. In nominally Ca2+-free medium, the sarco-endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin (1 μmol/L) increased [Ca2+]i from 68 to 241 nmol/L, and to 458 (P < 0.01) and 1006 nmol/L (P < 0.01), respectively, when 1.5 mmol/L and 3.0 mmol/L extracellular Ca2+ was reintroduced. Furthermore, the change in [Ca2+]i was observed with verapamil (5 μmol/L), La3+ (1 mmol/L) or KCl (40 mmol/L) in the bathing solution. These channels were sensitive to La3+ (P < 0.01), insensitive to verapamil, and voltage independent. In isolated distal colons we found that in normal Krebs solution, contraction induced by acetylcholine (ACh) was partially inhibited by verapamil, and the inhibitory rate was 41% (P < 0.05). On the other hand, in Ca2+-free Krebs solution, ACh induced transient contraction due to Ca2+ release from the intracellular stores. The transient contraction lasted until the Ca2+ store was depleted. Restoration of extracellular Ca2+ in the presence of atropine produced contraction, mainly due to Ca2+ influx. Such contraction was not inhibited by verapamil, but was decreased by La3+ (50 μmol/L) from 0.96 to 0.72 g (P < 0.01). CONCLUSION: The predominant source of activator Ca2+ for the contractile response to agonist is extracellular Ca2+, and intracellular Ca2+ has little role to play in mediating excitation-contraction coupling by agonists in rat distal colon smooth muscle in vitro. The influx of extracellular Ca2+ is mainly mediated through voltage-, receptor- and store-operated Ca2+ channels, which can be used as an alternative to develop new drugs targeted on the dysfunction of digestive tract motility.
基金Supported by National Natural Science Foundation of China,No.31271290 and No.31000514Scientific Research Key Program of Beijing Municipal Commission of Education No.KZ201310025020+1 种基金Beijing Postdoctoral Research FoundationBeijing Natural Science Foundation No.7142025
文摘AIM:To investigate whether Na+-K+-2Cl-cotransporter(NKCC2)is expressed in the mouse distal colonic epithelia and whether it is regulated by vasopressin in the colon.METHODS:The mRNA expression of NKCC2 in the mouse colonic mucosa was examined by reverse transcription-polymerase chain reaction.NKCC trafficking in the colon stimulated by 1-D-amino(8-D-arginine)-vasopressin(dDAVP)infusion(10 ng/mouse,intraperitoneal injection)within 15 min,30 min and 1h was investigated by laser confocal scanning microscopy.Total and membrane NKCC2 expression in the colonic mucosa from control and dDAVP-treated mice was detected by Western blotting.Short circuit current method was performed to determine regulation of NKCC2 by vasopressin in the colon.RESULTS:NKCC2 was predominantly located in the apical region of the surface of the distal colonic epithelia;by comparison,a large amount of NKCC1 was distributed in the basolateral membrane of the lower crypt epithelia of the mouse distal colon.Short-term treatment with dDAVP,a V2-type receptor-specific vasopressin analog,induced NKCC2 re-distribution,i.e.,NKCC2traffics to the apical membrane after dDAVP stimulation.In contrast,no obvious NKCC1 membrane translocation was observed.Western blotting results confirmed that membrane NKCC2 had significantly higher abundance in the dDAVP-treated mouse colonic mucosa relative to that in the untreated control,which is consistent with our immunostaining data.Moreover,the short-circuit current method combined with a NKCC2 inhibitor demonstrated that NKCC2 was also activated by serosal vasopressin in isolated distal colonic mucosa.CONCLUSION:Our results provide direct evidence that vasopressin also plays an important role in the colonic epithelia by stimulating NKCC2 trafficking to the apical membrane and inducing NKCC2-mediated ion transport.
基金Supported by grants from Ministerio de Ciencia y Tecnología (BFI 03/1350) and Fundacion MMA
文摘AIM: To investigate the effects of luminal exposure to H2O2 and two related thiol oxidizing agents on basal and stimulated chloride secretion in native colon using electro- physiological and pharmacological approaches. METHODS: Unstripped rat distal colon segments were mounted in Ussing chambers. Potential difference, cal- culated resistance and short-circuit current across un- stripped colon segments were monitored with a dual voltage/current clamp. Paracellular permeability was assessed by measuring the mucosa-to-serosa ? ux of a ? uorescent probe (FITC). RESULTS: Luminal exposure to hydrogen peroxide tran- sitorily stimulated chloride secretion without altering bar- rier function. This stimulatory effect could be blocked by basolateral atropine but not indomethacin. The cysteine and methionine oxidizing compounds, phenylarsine oxide and chloramine T respectively, mimicked the effect of H2O2, except for a drop in transcolonic resistance after 30 min. In contrast to the observed stimulatory effect on basal secretion, cAMP-stimulated electrogenic ion trans- port was blunted by luminal H2O2. However, the Ca2+- activated response remained unchanged. CONCLUSION: H2O2 may be an important selective modulator of intestinal ion and water secretion in certain pathologic conditions such as in? ammation or ischemia- reperfusion by multiple mechanisms.
文摘目的 探讨中药灌肠联合温针灸治疗远端结肠型溃疡性结肠炎的疗效。方法 将90例远端结肠型溃疡性结肠炎患者分为3组。在常规西药基础上,中药灌肠组予以中药灌肠,温针灸组予以温针灸治疗,联合治疗组则予以中药灌肠联合温针灸治疗。比较3组患者的临床疗效、生化指标及炎症因子水平、中医症状评分、结肠镜评分、结肠组织病理评分、黏膜愈合及复发情况、不良反应。结果 联合治疗组临床治疗总有效率显著高于中药灌肠组及温针灸组( P <0.05);联合治疗组治疗后白细胞计数(WBC)、血小板(PLT)、肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)水平均显著低于中药灌肠组及温针灸组( P <0.05);联合治疗组治疗后中医症状评分、结肠镜评分、结肠组织病理评分均显著低于中药灌肠组及温针灸组( P < 0.05);联合治疗组黏膜愈合率、复发率均显著优于中药灌肠组及温针灸组( P <0.05);3组不良反应发生率差异无统计学意义( P >0.05)。结论 中药灌肠联合温针灸治疗远端结肠型慢性溃疡性结肠炎的疗效显著,可显著改善中医症状,降低生化指标与炎症因子水平。
文摘目的研究多巴胺(DA)对大鼠结肠运动影响的机制。方法采用离体组织灌流方法记录大鼠远端结肠自发性节律运动,观察DA的作用以及阻断剂的影响,再用反转录实时多聚酶链反应(real time RT-PCR)检测受体基因的表达。结果DA(≥1.0×10-5mol/L)对结肠远端(紧接肛门淋巴结近端)离体纵行肌条(2.0 mm×10 mm)的运动具有抑制作用,多巴胺受体阻断剂(D1受体阻断剂SCH23390,1.0×10-7mol/L,D2受体阻断剂Sulpide,1.0×10-7mol/L)不能阻断多巴胺的抑制效应,但加入β3受体抑制剂cyanopindolol(7.5×10-7mol/L),DA的抑制作用显著减弱。real time RT-PCR检测发现β1、β2、β3受体mRNA在远端结肠均有表达。结论DA可通过β3受体发挥对远端结肠运动的抑制作用。
