Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effe...Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in Semen Euphorbiae on the viability of HEK293 cells were studied by MTT assay.The LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 h.The effect of the main diterpene ester components of Semen Euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western Blot.Results:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly down-regulated.Conclusion:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.展开更多
Aster souliei Franch, (Compositae) is a. herbaceous plant distributed innorth China. It has been used in folk medicine as antipyretic, detoxicant, expectorant andantitussive. In an effort to find biologically active c...Aster souliei Franch, (Compositae) is a. herbaceous plant distributed innorth China. It has been used in folk medicine as antipyretic, detoxicant, expectorant andantitussive. In an effort to find biologically active components from Chinese medicinal plants ' ,we have examined the aerial parts of this herb, leading to the isolation of a clerodane-typediterpene, 18, 19-dihydroxy-5α, 10β-neo-cleroda-3, 13 (l4)-dien-16, 15-butenolide (1). In thispaper we report the structural elucidation, and the antitumor and antibacterial activities of thiscompound. It was found that 1 possesses moderate cytotoxicity against human leukemia cells (HL-60)and activity against microorganisms.展开更多
Kiridine, a new C18-diterpenoid alkaloid, was isolated from the roots of Aconitum kirinense. Its structure was elucidated by MS, 1D- and 2D- NMR, and mono-crystal X-ray analysis. It is the first norditerpene alkaloid ...Kiridine, a new C18-diterpenoid alkaloid, was isolated from the roots of Aconitum kirinense. Its structure was elucidated by MS, 1D- and 2D- NMR, and mono-crystal X-ray analysis. It is the first norditerpene alkaloid containing a 9,14-methylenedioxy group and the substituent at C-14 is (-configuration.展开更多
A new cembranolide diterpene with a hydroperoxyl substitution was isolated from the marine soft coral Lobophytum crassum. The structure was elucidated on the basis of chemical and spectral methods.
Two new diterpenes, 2β, 16β-ent-kaurane diol, 15α, 16α-epoxy-17β-al-ent-kaurane- 19-oic acid were isolated from Helianthus annuus L. Their structures were determined by spectroscopic evidences.
目的观察银杏二萜内酯葡胺、丁苯酞注射液治疗老年急性缺血性脑卒中(Acute Ischemic Stroke,AIS)患者的临床疗效。方法选择2022年3月—2023年3月徐州市肿瘤医院收治的90例AIS患者为研究对象,按随机数表法分为两组,各45例。在常规治疗基...目的观察银杏二萜内酯葡胺、丁苯酞注射液治疗老年急性缺血性脑卒中(Acute Ischemic Stroke,AIS)患者的临床疗效。方法选择2022年3月—2023年3月徐州市肿瘤医院收治的90例AIS患者为研究对象,按随机数表法分为两组,各45例。在常规治疗基础上,对照组使用丁苯酞治疗,研究组则联用银杏二萜内酯葡胺与丁苯酞治疗。比较两组疗效、国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、血液流变学指标水平、不良反应。结果观察组治疗总有效率为91.11%,高于对照组的75.56%,差异有统计学意义(χ^(2)=3.920,P=0.048)。治疗后,研究组的NIHSS评分、血浆黏度、血浆黏度、纤维蛋白原、红细胞压积水平低于对照组,差异有统计学意义(P均<0.05)。两组不良反应总发生率比较,差异无统计学意义(P>0.05)。结论银杏二萜内酯葡胺联合丁苯酞注射液治疗老年AIS患者的临床疗效好,同时对血液流变学有良好的改善效果。展开更多
基金supported by National Natural Science Foundation of China(Grant No.82074021).
文摘Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in Semen Euphorbiae on the viability of HEK293 cells were studied by MTT assay.The LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 h.The effect of the main diterpene ester components of Semen Euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western Blot.Results:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly down-regulated.Conclusion:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.
文摘Aster souliei Franch, (Compositae) is a. herbaceous plant distributed innorth China. It has been used in folk medicine as antipyretic, detoxicant, expectorant andantitussive. In an effort to find biologically active components from Chinese medicinal plants ' ,we have examined the aerial parts of this herb, leading to the isolation of a clerodane-typediterpene, 18, 19-dihydroxy-5α, 10β-neo-cleroda-3, 13 (l4)-dien-16, 15-butenolide (1). In thispaper we report the structural elucidation, and the antitumor and antibacterial activities of thiscompound. It was found that 1 possesses moderate cytotoxicity against human leukemia cells (HL-60)and activity against microorganisms.
文摘Kiridine, a new C18-diterpenoid alkaloid, was isolated from the roots of Aconitum kirinense. Its structure was elucidated by MS, 1D- and 2D- NMR, and mono-crystal X-ray analysis. It is the first norditerpene alkaloid containing a 9,14-methylenedioxy group and the substituent at C-14 is (-configuration.
基金financed in part by the innovation program of the Institute of Oceanology,Chinese Academy of Sciences(IOCAS,L79032432)the program of"Bairen Jihua"awarded from the Chinese Academy of Sciences(to B.G.Wang)Y.P.Shi wishes thank the Key Laboratory of Experimental Marine Biology at IOCAS for partial financial support
文摘A new cembranolide diterpene with a hydroperoxyl substitution was isolated from the marine soft coral Lobophytum crassum. The structure was elucidated on the basis of chemical and spectral methods.
基金supported by the National Natural Science Foundation of China(No 20432030)
文摘Two new diterpenes, 2β, 16β-ent-kaurane diol, 15α, 16α-epoxy-17β-al-ent-kaurane- 19-oic acid were isolated from Helianthus annuus L. Their structures were determined by spectroscopic evidences.
文摘目的观察银杏二萜内酯葡胺、丁苯酞注射液治疗老年急性缺血性脑卒中(Acute Ischemic Stroke,AIS)患者的临床疗效。方法选择2022年3月—2023年3月徐州市肿瘤医院收治的90例AIS患者为研究对象,按随机数表法分为两组,各45例。在常规治疗基础上,对照组使用丁苯酞治疗,研究组则联用银杏二萜内酯葡胺与丁苯酞治疗。比较两组疗效、国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、血液流变学指标水平、不良反应。结果观察组治疗总有效率为91.11%,高于对照组的75.56%,差异有统计学意义(χ^(2)=3.920,P=0.048)。治疗后,研究组的NIHSS评分、血浆黏度、血浆黏度、纤维蛋白原、红细胞压积水平低于对照组,差异有统计学意义(P均<0.05)。两组不良反应总发生率比较,差异无统计学意义(P>0.05)。结论银杏二萜内酯葡胺联合丁苯酞注射液治疗老年AIS患者的临床疗效好,同时对血液流变学有良好的改善效果。
