Two New Steroidal Saponins from Diuranthera inarticulata

Two new steroidal saponins, diuranthosides D and E, were isolated from the whole plant of Diuranthera inarticulata Wang et K. Y. Lang. By means of spectral and chemical analysis, the structure of the new compounds were established as neotigogenin-3-O-(-D-glucopyranosyl (1?3)-(-D-xylopyranosyl-(1?3)-[(-D-glucopyranosyl(1?2)]-(-D-glucopyranosyl(1?4)-(-D-galactopyranoside (1) and neotigogenin-3-O-(-D-glucopyranosyl(1?3)-(-D-glucopyranosyl (1?3)-(-D-xylopyranosyl(1?3)-[(-D-glucopyranosyl(1?2)]-(-D-glucopyranosyl(1?4)-(-D-galactopyranoside (2) respectively.

鹭鸶兰的核型分析

本文报道了鹭鸶兰的染色体数目和核型。结果如下:鹭鸶兰2n=56=2M+12m+32sm+10st。

鹭鸶草组织培养再生体系的建立

为缓解鹭鸶草(Diuranthera major)野生资源短缺状况,以鹭鸶草花序轴为外植体,研究不同消毒方式对外植体无菌培养的影响以及不同培养基配方对鹭鸶草组织培养的影响。结果表明,流水冲洗20 min、酒精消毒10 s、浓度1/1000氯化汞消毒8 min,花序轴污染率为15.33%,成活率为68.67%;MS+2.0 mg/L 6-BA+0.25 mg/L NAA可促进花序轴直接诱导出不定芽,诱导率为93.33%;适宜的增殖配方为MS+1.50 mg/L 6-BA+0.10 mg/L NAA+0.75 mg/L KT,增殖系数为6.8;在MS培养基中添加20 g/L NH4NO3和20 g/L土豆汁有助于壮苗,叶色翠绿且舒展度高;生根培养基为1/2 MS+0.50 mg/L IBA+0.20 mg/L NAA,转入该培养基中16~17天即可生根,平均生根数7.94条,生根率100%。以鹭鸶草花序轴为外植体可建立高效快繁再生体系,实现无性系规模化育苗,移栽成活率达100%。