Relationships between microbial communities and geochemical environments are important in marine microbial ecology and biogeochemistry. Although biogeochemical redox stratification has been well documented in marine s...Relationships between microbial communities and geochemical environments are important in marine microbial ecology and biogeochemistry. Although biogeochemical redox stratification has been well documented in marine sediments, its impact on microbial communities remains largely unknown. In this study, we applied denaturing gradient gel electrophoresis (DGGE) and clone library construction to investigate the diversity and stratification of bacterial communities in redox-stratified sandy reef sediments in a microcosm. A total of 88 Operational Taxonomic Units (OTU) were identified from 16S rRNA clone libraries constructed from sandy reef sediments in a laboratory microcosm. They were members of nine phyla and three candidate divisions, including Proteobacteria (Alphas, Beta-, Gamma-, Delta-, and Epsilonproteobacteria), Aetinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, Verrucomicrobia, Spirochaetes, and the candidate divisions WS3, SO31 and AO19. The vast majority of these phylotypes are related to clone sequences from other marine sediments, but OTUs of Epsilonproteobacteria and WS3 are reported for the first time from permeable marine sediments. Several other OTUs are potential new bacterial phylotypes because of their low similarity with reference sequences. Results from the 16S rRNA, gene clone sequence analyses suggested that bacterial communities exhibit clear stratification across large redox gradients in these sediments, with the highest diversity found in the anoxic layer (15-25 mm) and the least diversity in the suboxic layer (3-5 mm). Analysis of the nosZ, and amoA gene libraries also indicated the stratification of denitrifiers and nitrifiers, with their highest diversity being in the anoxic and oxic sediment layers, respectively. These results indicated that redox-stratification can affect the distribution of bacterial communities in sandy reef sediments.展开更多
This article explores the impact of social work leadership integration and diversity and its response during the COVID-19 pandemic and its daily application.Social workers used various modalities to ensure that immedi...This article explores the impact of social work leadership integration and diversity and its response during the COVID-19 pandemic and its daily application.Social workers used various modalities to ensure that immediate priority was given to those individuals who were needed in various agencies and schools.Social workers provided added value and were essential to providing vital support through leadership skills in the development and operation during the COVID-19 pandemic.The social workers and leaders demonstrated the ability to intervene during the pandemic crisis through the infusion of a variety of leadership practices and integration.The profession itself makes social workers innate leaders.In the article,attention is given to the work that social workers embraced during the COVID-19 pandemic,to demonstrate that front-line workers are real experts.Social workers as leaders are guided by the code of ethics which employs a leadership style.展开更多
Integrated multi-trophic aquaculture(IMTA)has been considered as an ecofriendly culture system providing a potential solution to environmental risks caused by intensive monoculture system.However,the impact of IMTA on...Integrated multi-trophic aquaculture(IMTA)has been considered as an ecofriendly culture system providing a potential solution to environmental risks caused by intensive monoculture system.However,the impact of IMTA on phytoplankton remains unclear.In this study,the spatial and temporal variations of phytoplankton in Sanggou Bay were investigated seasonally based on 21 sampling sites covering three cultivation zones(bivalve zone,IMTA zone,and kelp zone)and one control zone(without aquatic cultivation).In total,128 phytoplankton species,with diatoms and dinoflagellates as the dominant groups,were obtained across the whole year,and the mean Shannon diversity index(H')and species richness(SR)were determined as 1.39 and 9.39,respectively.The maximum chlorophyll a(Chl-a)(6.32μg L^(-1))and plankton diversity(H'of 1.97)occurred in summer and autumn,respectively.Compared to other zones,the bivalve zone displayed significantly higher Chl-a and lower H'in majority of time.Pairwise PERMANOVA analysis indicated that the phytoplankton assemblage in the bivalve zone was significantly different with the control and kelp zones,while the IMTA zone maintained close to other three zones.Based on generalized additive models,temperature,NO_(2)^(-)-N,N/P ratio,SiO_(3)^(2-)-Si,and salinity were determined as the key factors underlying Chl-a and phytoplankton diversity.Addi-tionally,the results of redundancy analysis further indicated that the phytoplankton assemblage in the bivalve zone is positively re-lated with nutrients such as NO_(3)^(-)-N and NH_(4)^(+)-N as well as water depth,while the phytoplankton assemblages in the kelp,control,and IMTA zones are associated with NO_(2)^(-)-N,SiO_(3)^(2-)-Si,and salinity.Taken all observations into consideration together,it can be inferred that IMTA can effectively reduce Chl-a level compared to bivalve monoculture by reducing the nutrients.However,the SR,H’,and species composition of phytoplankton are primarily determined by local environment factors such as temperature,water depth,salinity and SiO_(3)^(2-)-Si.展开更多
In this paper we describe an integrated collector storage solar water heater for the North Western region of India for use as domestic water heater during the winter season and present the experimental results of temp...In this paper we describe an integrated collector storage solar water heater for the North Western region of India for use as domestic water heater during the winter season and present the experimental results of temperature stratification. The system consists of a steel water storage tank with azimuthal orientation such that its walls face south east, south west, north east and North West directions. The sunlit walls (south east and south west) and the top cover surface of the storage tank are covered with transparent insulation material (TIM) and the off-sunlit sides with opaque insulation. Experimental results show that the top layer is at the highest temperature. The top layer is drained using a new design of the outlet. The system can be used in rural areas or as a pre heater in more affluent households. The system can return the cost of conversion of the storage tank into a Solar Water Heater in one winter season by saving the cost of electrical energy required for heating water during winter months.展开更多
Utilizing both borehole and Cone Penetration Testing(CPT)data in soil stratification helps to get more convincing soil stratification results.However,the soil classification results revealed by borehole(Unified Soil C...Utilizing both borehole and Cone Penetration Testing(CPT)data in soil stratification helps to get more convincing soil stratification results.However,the soil classification results revealed by borehole(Unified Soil Classification System,USCS)and CPT tests(soil behavior type,SBT)are commonly not con-sistent.This study proposes a feasible solution to integrate the borehole and CPT data with the tree-based method.The tree-based method is naturally suitable for soil stratification tasks as it aims to divide the subsurface space into several clusters based on the similarities of the soil types.A novel boundary dic-tionary method is proposed to enhance the model performance on complex soil layer conditions.A prob-abilistic mapping matrix between the USCS-SBT system is built based on a collected municipal database with collocated borehole and CPT data.The optimal soil stratification results can be selected based on considering multiple borehole information and pruning the structure of trees.The structure of the trees can be optimized in a back analysis perspective with the Sequential Model-Based Global Optimization(SMBO)algorithm which aims to maximize the possibility of observing the borehole information based on the USCS-SBT probabilistic mapping matrix.The uncertainties of the optimal soil stratification results can be estimated based on a weighted Gini index method.The performance of the proposed method is validated based on a real case in New Zealand with a cross-validation method.The results indicate that the proposed method is robust and effective.展开更多
针对影子栈难以兼顾安全性和性能,软件实现的前向CFI(control-flow integrity)技术性能开销过大、难以进行实际部署,提出基于软硬件结合的CFI(control-flow integrity based on the combination of software and hardware,SHCFI)技术。...针对影子栈难以兼顾安全性和性能,软件实现的前向CFI(control-flow integrity)技术性能开销过大、难以进行实际部署,提出基于软硬件结合的CFI(control-flow integrity based on the combination of software and hardware,SHCFI)技术。通过二进制重写器对程序反编译,生成中间语言,并判断转移指令类型。对于后向控制流的保护,提出基于返回地址加密的平行影子栈方案,使用随机数对栈上的返回地址进行异或加密,将加密结果备份到原始栈固定偏移处的影子栈中,在函数返回时对影子栈中的地址异或解密,将解密结果作为实际的返回地址。对于前向控制流的保护,使用硬件ENDBRANCH状态机指令标记间接转移指令的目标地址,在运行时对目标地址进行合法性检查,以降低性能开销。实验结果表明,SHCFI加固后的程序能够有效地防御代码重用攻击,且具有良好的运行开销。展开更多
基金Supported by a NOAA Grant(No.NA04OAR4600196(GW))the microcosm development and operation was supported by the U.S.National Science Foundation(Nos.OCE03-27332 and OCE05-36616(FJS))a project of Shandong Province Higher Education Science and Technology Program(No.J10LC09)
文摘Relationships between microbial communities and geochemical environments are important in marine microbial ecology and biogeochemistry. Although biogeochemical redox stratification has been well documented in marine sediments, its impact on microbial communities remains largely unknown. In this study, we applied denaturing gradient gel electrophoresis (DGGE) and clone library construction to investigate the diversity and stratification of bacterial communities in redox-stratified sandy reef sediments in a microcosm. A total of 88 Operational Taxonomic Units (OTU) were identified from 16S rRNA clone libraries constructed from sandy reef sediments in a laboratory microcosm. They were members of nine phyla and three candidate divisions, including Proteobacteria (Alphas, Beta-, Gamma-, Delta-, and Epsilonproteobacteria), Aetinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, Verrucomicrobia, Spirochaetes, and the candidate divisions WS3, SO31 and AO19. The vast majority of these phylotypes are related to clone sequences from other marine sediments, but OTUs of Epsilonproteobacteria and WS3 are reported for the first time from permeable marine sediments. Several other OTUs are potential new bacterial phylotypes because of their low similarity with reference sequences. Results from the 16S rRNA, gene clone sequence analyses suggested that bacterial communities exhibit clear stratification across large redox gradients in these sediments, with the highest diversity found in the anoxic layer (15-25 mm) and the least diversity in the suboxic layer (3-5 mm). Analysis of the nosZ, and amoA gene libraries also indicated the stratification of denitrifiers and nitrifiers, with their highest diversity being in the anoxic and oxic sediment layers, respectively. These results indicated that redox-stratification can affect the distribution of bacterial communities in sandy reef sediments.
文摘This article explores the impact of social work leadership integration and diversity and its response during the COVID-19 pandemic and its daily application.Social workers used various modalities to ensure that immediate priority was given to those individuals who were needed in various agencies and schools.Social workers provided added value and were essential to providing vital support through leadership skills in the development and operation during the COVID-19 pandemic.The social workers and leaders demonstrated the ability to intervene during the pandemic crisis through the infusion of a variety of leadership practices and integration.The profession itself makes social workers innate leaders.In the article,attention is given to the work that social workers embraced during the COVID-19 pandemic,to demonstrate that front-line workers are real experts.Social workers as leaders are guided by the code of ethics which employs a leadership style.
基金supported by the National Science and Technology Basic Resources Investigation Program of China(No.2018FY100206)the National Natural Science Foundation of China(Nos.31902370 and 42276099)+2 种基金the Ningbo Public Welfare Science and Technology Program(No.2022S161)the Key Program of Science and Technology Innovation in Ningbo(No.2023Z118)the National Key Research and Development Program of China(No.2018YFD0900703).
文摘Integrated multi-trophic aquaculture(IMTA)has been considered as an ecofriendly culture system providing a potential solution to environmental risks caused by intensive monoculture system.However,the impact of IMTA on phytoplankton remains unclear.In this study,the spatial and temporal variations of phytoplankton in Sanggou Bay were investigated seasonally based on 21 sampling sites covering three cultivation zones(bivalve zone,IMTA zone,and kelp zone)and one control zone(without aquatic cultivation).In total,128 phytoplankton species,with diatoms and dinoflagellates as the dominant groups,were obtained across the whole year,and the mean Shannon diversity index(H')and species richness(SR)were determined as 1.39 and 9.39,respectively.The maximum chlorophyll a(Chl-a)(6.32μg L^(-1))and plankton diversity(H'of 1.97)occurred in summer and autumn,respectively.Compared to other zones,the bivalve zone displayed significantly higher Chl-a and lower H'in majority of time.Pairwise PERMANOVA analysis indicated that the phytoplankton assemblage in the bivalve zone was significantly different with the control and kelp zones,while the IMTA zone maintained close to other three zones.Based on generalized additive models,temperature,NO_(2)^(-)-N,N/P ratio,SiO_(3)^(2-)-Si,and salinity were determined as the key factors underlying Chl-a and phytoplankton diversity.Addi-tionally,the results of redundancy analysis further indicated that the phytoplankton assemblage in the bivalve zone is positively re-lated with nutrients such as NO_(3)^(-)-N and NH_(4)^(+)-N as well as water depth,while the phytoplankton assemblages in the kelp,control,and IMTA zones are associated with NO_(2)^(-)-N,SiO_(3)^(2-)-Si,and salinity.Taken all observations into consideration together,it can be inferred that IMTA can effectively reduce Chl-a level compared to bivalve monoculture by reducing the nutrients.However,the SR,H’,and species composition of phytoplankton are primarily determined by local environment factors such as temperature,water depth,salinity and SiO_(3)^(2-)-Si.
文摘In this paper we describe an integrated collector storage solar water heater for the North Western region of India for use as domestic water heater during the winter season and present the experimental results of temperature stratification. The system consists of a steel water storage tank with azimuthal orientation such that its walls face south east, south west, north east and North West directions. The sunlit walls (south east and south west) and the top cover surface of the storage tank are covered with transparent insulation material (TIM) and the off-sunlit sides with opaque insulation. Experimental results show that the top layer is at the highest temperature. The top layer is drained using a new design of the outlet. The system can be used in rural areas or as a pre heater in more affluent households. The system can return the cost of conversion of the storage tank into a Solar Water Heater in one winter season by saving the cost of electrical energy required for heating water during winter months.
基金funded by the Australian Government through the Australian Research Council’s Discovery Projects funding scheme (Project DP190101592)the National Natural Science Foundation of China (No.41972280,52179103).
文摘Utilizing both borehole and Cone Penetration Testing(CPT)data in soil stratification helps to get more convincing soil stratification results.However,the soil classification results revealed by borehole(Unified Soil Classification System,USCS)and CPT tests(soil behavior type,SBT)are commonly not con-sistent.This study proposes a feasible solution to integrate the borehole and CPT data with the tree-based method.The tree-based method is naturally suitable for soil stratification tasks as it aims to divide the subsurface space into several clusters based on the similarities of the soil types.A novel boundary dic-tionary method is proposed to enhance the model performance on complex soil layer conditions.A prob-abilistic mapping matrix between the USCS-SBT system is built based on a collected municipal database with collocated borehole and CPT data.The optimal soil stratification results can be selected based on considering multiple borehole information and pruning the structure of trees.The structure of the trees can be optimized in a back analysis perspective with the Sequential Model-Based Global Optimization(SMBO)algorithm which aims to maximize the possibility of observing the borehole information based on the USCS-SBT probabilistic mapping matrix.The uncertainties of the optimal soil stratification results can be estimated based on a weighted Gini index method.The performance of the proposed method is validated based on a real case in New Zealand with a cross-validation method.The results indicate that the proposed method is robust and effective.
文摘针对影子栈难以兼顾安全性和性能,软件实现的前向CFI(control-flow integrity)技术性能开销过大、难以进行实际部署,提出基于软硬件结合的CFI(control-flow integrity based on the combination of software and hardware,SHCFI)技术。通过二进制重写器对程序反编译,生成中间语言,并判断转移指令类型。对于后向控制流的保护,提出基于返回地址加密的平行影子栈方案,使用随机数对栈上的返回地址进行异或加密,将加密结果备份到原始栈固定偏移处的影子栈中,在函数返回时对影子栈中的地址异或解密,将解密结果作为实际的返回地址。对于前向控制流的保护,使用硬件ENDBRANCH状态机指令标记间接转移指令的目标地址,在运行时对目标地址进行合法性检查,以降低性能开销。实验结果表明,SHCFI加固后的程序能够有效地防御代码重用攻击,且具有良好的运行开销。