Microglia-mediated neuroinflammation is widely perceived as a contributor to numerous neurological diseases and mental disorders including depression.Discs large homolog 1(Dlg1),an adaptor protein,regulates cell polar...Microglia-mediated neuroinflammation is widely perceived as a contributor to numerous neurological diseases and mental disorders including depression.Discs large homolog 1(Dlg1),an adaptor protein,regulates cell polarization and the function of K?channels,which are reported to regulate the activation of microglia.However,little is known about the role of Dlg1 in microglia and the maintenance of central nervous system homeostasis.In this study,we found that Dlg1 knockdown suppressed lipopolysaccharide(LPS)-induced inflammation by downregulating the activation of nuclear factor-jB signaling and the mitogen-activated protein kinase pathway in microglia.Moreover,using an inducible Dlg1 microglia-specific knockout(Dlg1flox/flox;CX3CR1CreER)mouse line,we found that microglial Dlg1 knockout reduced the activation of microglia and alleviated the LPS-induced depressionlike behavior.In summary,our results demonstrated that Dlg1 plays a critical role in microglial activation and thus provides a potential therapeutic target for the clinical treatment of depression.展开更多
研究了大比例尺地形图在1∶10000数字线划图(Digital Line Graph,DLG)数学精度检测中的应用。笔者先从政府权威部门收集了覆盖检测区域的现势性强且比例尺较大的基础地理数据,然后利用ArcGIS软件实现大比例尺地形图坐标系统向CGCS2000的...研究了大比例尺地形图在1∶10000数字线划图(Digital Line Graph,DLG)数学精度检测中的应用。笔者先从政府权威部门收集了覆盖检测区域的现势性强且比例尺较大的基础地理数据,然后利用ArcGIS软件实现大比例尺地形图坐标系统向CGCS2000的3°分带高斯坐标系的严格转换。叠加2套数据,根据检测要求与流程精准配对其平面位置和高程检测要素,并分别进行精度评定。结果表明,基于大比例尺地形图的1∶10000 DLG数学精度检测方法满足现有国家规范的要求,结果可靠,且与传统检测方法相比,极大地减少了测量人员和仪器设备的投入,检测周期大幅缩短,工作效率显著提高,值得应用与推广。展开更多
基金the National Natural Science Foundation of China(82071218,81630026,and 81930029).
文摘Microglia-mediated neuroinflammation is widely perceived as a contributor to numerous neurological diseases and mental disorders including depression.Discs large homolog 1(Dlg1),an adaptor protein,regulates cell polarization and the function of K?channels,which are reported to regulate the activation of microglia.However,little is known about the role of Dlg1 in microglia and the maintenance of central nervous system homeostasis.In this study,we found that Dlg1 knockdown suppressed lipopolysaccharide(LPS)-induced inflammation by downregulating the activation of nuclear factor-jB signaling and the mitogen-activated protein kinase pathway in microglia.Moreover,using an inducible Dlg1 microglia-specific knockout(Dlg1flox/flox;CX3CR1CreER)mouse line,we found that microglial Dlg1 knockout reduced the activation of microglia and alleviated the LPS-induced depressionlike behavior.In summary,our results demonstrated that Dlg1 plays a critical role in microglial activation and thus provides a potential therapeutic target for the clinical treatment of depression.
文摘研究了大比例尺地形图在1∶10000数字线划图(Digital Line Graph,DLG)数学精度检测中的应用。笔者先从政府权威部门收集了覆盖检测区域的现势性强且比例尺较大的基础地理数据,然后利用ArcGIS软件实现大比例尺地形图坐标系统向CGCS2000的3°分带高斯坐标系的严格转换。叠加2套数据,根据检测要求与流程精准配对其平面位置和高程检测要素,并分别进行精度评定。结果表明,基于大比例尺地形图的1∶10000 DLG数学精度检测方法满足现有国家规范的要求,结果可靠,且与传统检测方法相比,极大地减少了测量人员和仪器设备的投入,检测周期大幅缩短,工作效率显著提高,值得应用与推广。
