Pseudoalteromonas sp. SM9913 is a phychrotmphic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL - PCR (GenBank accession ...Pseudoalteromonas sp. SM9913 is a phychrotmphic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL - PCR (GenBank accession Nos DQ640312, DQ504163 ). The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria, and show some cold-adapted characteristics in their structures when compared with those from psychro-, meso-and themophilic bacteria. It is indicated that chaperones DnaJ and DnaK of P. sp. SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding, assembling and translocation of proteins at low temperature. This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms.展开更多
通过对大豆铝胁迫下的转录组测序分析,发现一个差异表达的基因,其编码一个具有101个氨基酸残基的Dna J-like分子伴侣蛋白-Gm Dna J1(Glycine max Dna J1),等电点为8.97;序列分析表明该蛋白具有典型的高度保守的J domain功能域,是一种类...通过对大豆铝胁迫下的转录组测序分析,发现一个差异表达的基因,其编码一个具有101个氨基酸残基的Dna J-like分子伴侣蛋白-Gm Dna J1(Glycine max Dna J1),等电点为8.97;序列分析表明该蛋白具有典型的高度保守的J domain功能域,是一种类型III的J蛋白;通过对其序列的同源性及进化关系分析,推测该蛋白可能响应重金属胁迫。为进一步探究Gm Dna J1是否能够对重金属胁迫产生应答反应,试验分别以0或100μmol·L-1Cu2+、Pb2+和Cd2+溶液胁迫处理的不同时间(0、12、24、48和72 h)大豆根尖RNA为材料,通过实时定量PCR研究了该蛋白基因的表达特征。结果表明:与对照相比,Gm Dna J1受Cu、Pb和Cd等重金属的诱导而强烈表达,呈现先升高后降低的趋势,其中Pb、Cd处理24 h后表达水平达到峰值,而Cu处理48 h后达到峰值;此外,Gm Dna J1对Cu、Pb和Cd胁迫的响应程度也不同,表明该基因对这三种重金属的响应模式存在差异。根据以上研究结果,推测大豆Gm Dna J1蛋白不仅响应铝毒胁迫,而且可能在响应重金属胁迫方面具有重要的作用,参与了大豆对重金属毒害的抵抗。该结果为深入研究Gm Dna J1在重金属胁迫响应中的功能及其分子机制提供了一定的依据。展开更多
基金The work was supported by the Hi-Tech Research and Development Program of China under contract Nos 2006AA09Z414 and 2007AA091903;the China Ocean Mineral Resources R & D Association under contract No. DYXM - 115 - 02 - 2 - 6;the National Natural Science Foundation of China under contract No. Z2004D02;the Natural Science Foundation of Shandong Province of China under contract No. Z2004D02;the Foundation for Young Excellent Scientists in Shandong Province of China under contract No. 2006BS02002;the Program for New Century Excellent Talents in University under contract No. NCET - 06 - 0578.
文摘Pseudoalteromonas sp. SM9913 is a phychrotmphic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL - PCR (GenBank accession Nos DQ640312, DQ504163 ). The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria, and show some cold-adapted characteristics in their structures when compared with those from psychro-, meso-and themophilic bacteria. It is indicated that chaperones DnaJ and DnaK of P. sp. SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding, assembling and translocation of proteins at low temperature. This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms.
文摘通过对大豆铝胁迫下的转录组测序分析,发现一个差异表达的基因,其编码一个具有101个氨基酸残基的Dna J-like分子伴侣蛋白-Gm Dna J1(Glycine max Dna J1),等电点为8.97;序列分析表明该蛋白具有典型的高度保守的J domain功能域,是一种类型III的J蛋白;通过对其序列的同源性及进化关系分析,推测该蛋白可能响应重金属胁迫。为进一步探究Gm Dna J1是否能够对重金属胁迫产生应答反应,试验分别以0或100μmol·L-1Cu2+、Pb2+和Cd2+溶液胁迫处理的不同时间(0、12、24、48和72 h)大豆根尖RNA为材料,通过实时定量PCR研究了该蛋白基因的表达特征。结果表明:与对照相比,Gm Dna J1受Cu、Pb和Cd等重金属的诱导而强烈表达,呈现先升高后降低的趋势,其中Pb、Cd处理24 h后表达水平达到峰值,而Cu处理48 h后达到峰值;此外,Gm Dna J1对Cu、Pb和Cd胁迫的响应程度也不同,表明该基因对这三种重金属的响应模式存在差异。根据以上研究结果,推测大豆Gm Dna J1蛋白不仅响应铝毒胁迫,而且可能在响应重金属胁迫方面具有重要的作用,参与了大豆对重金属毒害的抵抗。该结果为深入研究Gm Dna J1在重金属胁迫响应中的功能及其分子机制提供了一定的依据。