“Breeding by design” for pure lines may be achieved by construction of an additive QTL-allele matrix in a germplasm panel or breeding population, but this option is not available for hybrids, where both additive and...“Breeding by design” for pure lines may be achieved by construction of an additive QTL-allele matrix in a germplasm panel or breeding population, but this option is not available for hybrids, where both additive and dominance QTL-allele matrices must be constructed. In this study, a hybrid-QTL identification approach, designated PLSRGA, using partial least squares regression(PLSR) for model fitting integrated with a genetic algorithm(GA) for variable selection based on a multi-locus, multi-allele model is described for additive and dominance QTL-allele detection in a diallel hybrid population(DHP). The PLSRGA was shown by simulation experiments to be superior to single-marker analysis and was then used for QTL-allele identification in a soybean DPH yield experiment with eight parents. Twenty-eight main-effect QTL with 138 alleles and nine QTL × environment QTL with 46 alleles were identified, with respective contributions of 61.8% and 23.5% of phenotypic variation. Main-effect additive and dominance QTL-allele matrices were established as a compact form of the DHP genetic structure. The mechanism of heterosis superior-to-parents(or superior-to-parents heterosis, SPH) was explored and might be explained by a complementary locus-set composed of OD+(showing positive over-dominance, most often), PD+(showing positive partial-to-complete dominance, less often) and HA+(showing positive homozygous additivity, occasionally) loci, depending on the parental materials. Any locus-type, whether OD+, PD + and HA+, could be the best genotype of a locus. All hybrids showed various numbers of better or best genotypes at many but not necessarily all loci, indicating further SPH improvement. Based on the additive/dominance QTL-allele matrices, the best hybrid genotype was predicted, and a hybrid improvement approach is suggested. PLSRGA is powerful for hybrid QTL-allele detection and cross-SPH improvement.展开更多
The study on raising of strong tomato seedlings in high-temperature season is of great practical significance,because high-temperature condition in summer and autumn causes excessive growth of tomato seedlings easily....The study on raising of strong tomato seedlings in high-temperature season is of great practical significance,because high-temperature condition in summer and autumn causes excessive growth of tomato seedlings easily. ‘Fenniya'variety was selected as an experimental material,an orthogonal experiment was designed with rhizospheric temperature( A),light intensity( B) and nutrient salt concentration( C) as three factors which were designed with three levels,so as to study the effects of different treatments on root activity,strong seedling index,hypocotyl length and average internode length. The results showed that the most important factor for tomato seedling's root activity,hypocotyl length and internode length was nutrient salt concentration. In terms of effect on seedling index,substrate temperature should be reduced properly,to avoid excessive shading. It was primarily determined that the effects of three factors on the raising of strong tomato seedlings ranked as factor C,factor B and factor A from high to low; and the screened optimal seedling raising conditions were the rhizospheric temperature at( 3 + 1)℃,the shading rate of( 50 + 5) % and the EC value of( 5. 0 + 0. 5) mS/cm.展开更多
The orthogonal Latin hypercube design and its relaxation,and column-orthogonal design,are two kinds of orthogonal designs for computer experiments.However,they usually do not achieve maximum stratifications in multi-d...The orthogonal Latin hypercube design and its relaxation,and column-orthogonal design,are two kinds of orthogonal designs for computer experiments.However,they usually do not achieve maximum stratifications in multi-dimensional margins.In this paper,we propose some methods to construct column-orthogonal designs with multi-dimensional stratifications by rotating symmetric and asymmetric orthogonal arrays.The newly constructed column-orthogonal designs ensure that the estimates of all linear effects are uncorrelated with each other and even uncorrelated with the estimates of all second-order effects(quadratic effects and bilinear effects)when the rotated orthogonal arrays have strength larger than two.Besides orthogonality,the resulting designs also preserve better space-filling properties than those constructed by using the existing methods.In addition,we provide a method to construct a new class of orthogonal Latin hypercube designs with multi-dimensional stratifications by rotating regular factorial designs.Some newly constructed orthogonal Latin hypercube designs are tabulated for practical use.展开更多
A comprehensive study of the tribological performance of the Al-Zn-Mg-Cu/Al2O3 composite and its matrix alloy is presented in this paper, with a specific emphasis to identify and model the applicable wear conditions w...A comprehensive study of the tribological performance of the Al-Zn-Mg-Cu/Al2O3 composite and its matrix alloy is presented in this paper, with a specific emphasis to identify and model the applicable wear conditions where the composite provides a minimum of 50% reduction in wear rate and 25% lowering of the friction coefficient. Two-body abrasion experiments following Taguchi L27 orthogonal design have been performed separately on alloy and composite materials, both prepared by the stir casting method. The influence of crucial control factors including silicon carbide (SiC) abrasive size, load, sliding distance, and velocity on the percentage variations of wear rates and friction coefficients between alloy and composite have been studied using the analysis of variance technique and full quadratic regression method. The dominant control factors are identified as abrasive size, load, and the interaction between abrasive size and load. This has been verified by establishing the influence of abrasive size and load on variations of wear mechanisms like microcutting, microploughing, and delamination, identified by means of in-depth characterization of worn surfaces and generated debris for both alloy and composite. The selection of applicable tribological condition for the composite has been accomplished by adopting the multi-response optimization technique based on combined desirability approach to obtain concurrent optimization of the percentage variations of wear rates and friction coefficients. Predictive models correlating the superiority of tribological performance of composite with abrasion conditions have been developed, and these are found to be accurate (errors <10%), as determined by confirmatory experiment.展开更多
基金supported by the National Key Research and Development Program of China (2021YFF1001204,2017YFD0101500)the MOE Program of Introducing Talents of Discipline to Universities (“111”Project, B08025)+4 种基金the MOE Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT_17R55)the MARA CARS-04 Programthe Jiangsu Higher Education PAPD Programthe Fundamental Research Funds for the Central Universities (KYZZ201901)the Jiangsu JCICMCP Program。
文摘“Breeding by design” for pure lines may be achieved by construction of an additive QTL-allele matrix in a germplasm panel or breeding population, but this option is not available for hybrids, where both additive and dominance QTL-allele matrices must be constructed. In this study, a hybrid-QTL identification approach, designated PLSRGA, using partial least squares regression(PLSR) for model fitting integrated with a genetic algorithm(GA) for variable selection based on a multi-locus, multi-allele model is described for additive and dominance QTL-allele detection in a diallel hybrid population(DHP). The PLSRGA was shown by simulation experiments to be superior to single-marker analysis and was then used for QTL-allele identification in a soybean DPH yield experiment with eight parents. Twenty-eight main-effect QTL with 138 alleles and nine QTL × environment QTL with 46 alleles were identified, with respective contributions of 61.8% and 23.5% of phenotypic variation. Main-effect additive and dominance QTL-allele matrices were established as a compact form of the DHP genetic structure. The mechanism of heterosis superior-to-parents(or superior-to-parents heterosis, SPH) was explored and might be explained by a complementary locus-set composed of OD+(showing positive over-dominance, most often), PD+(showing positive partial-to-complete dominance, less often) and HA+(showing positive homozygous additivity, occasionally) loci, depending on the parental materials. Any locus-type, whether OD+, PD + and HA+, could be the best genotype of a locus. All hybrids showed various numbers of better or best genotypes at many but not necessarily all loci, indicating further SPH improvement. Based on the additive/dominance QTL-allele matrices, the best hybrid genotype was predicted, and a hybrid improvement approach is suggested. PLSRGA is powerful for hybrid QTL-allele detection and cross-SPH improvement.
基金Supported by Special Funding Project for the Construction of Henan Modern Agricultural Industrial Technology System(S2010-03)
文摘The study on raising of strong tomato seedlings in high-temperature season is of great practical significance,because high-temperature condition in summer and autumn causes excessive growth of tomato seedlings easily. ‘Fenniya'variety was selected as an experimental material,an orthogonal experiment was designed with rhizospheric temperature( A),light intensity( B) and nutrient salt concentration( C) as three factors which were designed with three levels,so as to study the effects of different treatments on root activity,strong seedling index,hypocotyl length and average internode length. The results showed that the most important factor for tomato seedling's root activity,hypocotyl length and internode length was nutrient salt concentration. In terms of effect on seedling index,substrate temperature should be reduced properly,to avoid excessive shading. It was primarily determined that the effects of three factors on the raising of strong tomato seedlings ranked as factor C,factor B and factor A from high to low; and the screened optimal seedling raising conditions were the rhizospheric temperature at( 3 + 1)℃,the shading rate of( 50 + 5) % and the EC value of( 5. 0 + 0. 5) mS/cm.
基金supported by National Natural Science Foundation of China(Grant Nos.11871033,11771220,11671386,11771219 and 11971345)National Ten Thousand Talents Program+2 种基金Tianjin Development Program for Innovation and EntrepreneurshipTianjin“131”Talents Programthe Postdoctoral Science Foundation Funded Project of China(Grant No.2017M611147)。
文摘The orthogonal Latin hypercube design and its relaxation,and column-orthogonal design,are two kinds of orthogonal designs for computer experiments.However,they usually do not achieve maximum stratifications in multi-dimensional margins.In this paper,we propose some methods to construct column-orthogonal designs with multi-dimensional stratifications by rotating symmetric and asymmetric orthogonal arrays.The newly constructed column-orthogonal designs ensure that the estimates of all linear effects are uncorrelated with each other and even uncorrelated with the estimates of all second-order effects(quadratic effects and bilinear effects)when the rotated orthogonal arrays have strength larger than two.Besides orthogonality,the resulting designs also preserve better space-filling properties than those constructed by using the existing methods.In addition,we provide a method to construct a new class of orthogonal Latin hypercube designs with multi-dimensional stratifications by rotating regular factorial designs.Some newly constructed orthogonal Latin hypercube designs are tabulated for practical use.
基金The authors recognize the support obtained from the Centre of Excellence on Microstructurally Designed Advanced Materials Development under TEQIP-II,IIEST Shibpur.The assistance granted from DST-FIST to set up Advanced Tribology Laboratory under the Department of Mechanical Engineering,IIEST Shibpur is also highly appreciated.
文摘A comprehensive study of the tribological performance of the Al-Zn-Mg-Cu/Al2O3 composite and its matrix alloy is presented in this paper, with a specific emphasis to identify and model the applicable wear conditions where the composite provides a minimum of 50% reduction in wear rate and 25% lowering of the friction coefficient. Two-body abrasion experiments following Taguchi L27 orthogonal design have been performed separately on alloy and composite materials, both prepared by the stir casting method. The influence of crucial control factors including silicon carbide (SiC) abrasive size, load, sliding distance, and velocity on the percentage variations of wear rates and friction coefficients between alloy and composite have been studied using the analysis of variance technique and full quadratic regression method. The dominant control factors are identified as abrasive size, load, and the interaction between abrasive size and load. This has been verified by establishing the influence of abrasive size and load on variations of wear mechanisms like microcutting, microploughing, and delamination, identified by means of in-depth characterization of worn surfaces and generated debris for both alloy and composite. The selection of applicable tribological condition for the composite has been accomplished by adopting the multi-response optimization technique based on combined desirability approach to obtain concurrent optimization of the percentage variations of wear rates and friction coefficients. Predictive models correlating the superiority of tribological performance of composite with abrasion conditions have been developed, and these are found to be accurate (errors <10%), as determined by confirmatory experiment.