'Dignity'probably isn’t the first word that comes to mind when you spot a gang of Beijing Pekinese,or京巴(Jingba),pulling their owners down the street.With their snubbed-nose faces,lolling tongues and patteri...'Dignity'probably isn’t the first word that comes to mind when you spot a gang of Beijing Pekinese,or京巴(Jingba),pulling their owners down the street.With their snubbed-nose faces,lolling tongues and pattering gait,the ubiquitous lap dog looks as much like an overgrown rodent as it does a prized purebred.But behind their befuddled expressions,these pitiful pups hide an ancient royal lineage.The Pekinese’s original long-haired ancestors were carefully bred to展开更多
“狗咬人”虽不是新闻。但是整个故事都围绕着被咬者的耳朵展开。一波三折,让人关注。作者蕴幽默于不动声色的行文中。非常耐读。比如:The dog survived, but the ear didn't. When it finally reached the hospital four hours aft...“狗咬人”虽不是新闻。但是整个故事都围绕着被咬者的耳朵展开。一波三折,让人关注。作者蕴幽默于不动声色的行文中。非常耐读。比如:The dog survived, but the ear didn't. When it finally reached the hospital four hours after being chewed off, it was no longer in any condition to be stitched back on. 动词survived值得读者玩味,而且。When it finally reached the hospital…一句的中的主语it,不是别的而是the ear!我们汉语就不能直译,如译:当耳朵来到医院的时候……】展开更多
Objective To develop and validate a simple, rapid, sensitive, and reproducible HPLC method for determination of hyperoside in plasma of dogs and for the subsequent pharmacokinetic (PK) study. Methods An accurate and r...Objective To develop and validate a simple, rapid, sensitive, and reproducible HPLC method for determination of hyperoside in plasma of dogs and for the subsequent pharmacokinetic (PK) study. Methods An accurate and reproducible HPLC-UV method was developed and validated for the determination of hyperoside in plasma of dogs, using kaempferol as internal standard. The plasma samples of dogs following ig administration of hyperoside were analyzed for the detection of quercetin after enzymatic hydrolysis treatment with combined β-glucuronidase and sulphatase. The analytes were separated on a Diamonsil C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-buffer solution (0.1 mol/L NH 4 Ac + 0.3 mmol/L EDTA-Na 2 )-acetic acid (60:40:1) and was delivered at a flow rate of 1 mL/min. The UV detector was set at 370 nm and the column temperature was maintained at 35 ℃. The sample injection volume was 20 μL. Data were collected and analyzed using the ANASTAR software. PK parameters were calculated with DAS software (2.0). Results Linear relationships were validated over the range of 0.01-1 μg/mL for hyperoside (r = 0.9997). The intra- and inter-day precision values for all samples were within 10.0%, and the accuracies of intra- and inter-day assays were within the range of 92.4%-102.4%. The validated method was successfully used to determine the hyperoside concentration in plasma of dogs for up to 12 h, after a single ig administration (25 mg/kg). The mean PK parameters for male and female dogs were as follows: C max (0.18 ± 0.05) and (0.16 ± 0.05) μg/mL, AUC 0-∞ (0.79 ± 0.34) and (0.86 ± 0.27) μg/(mL·h), t 1/2(ka) (0.89 ± 0.41) and (0.88 ± 0.28) h, respectively. Statistical analysis on the PK of hyperoside in male and female groups showed that sex had no significant impact on the PK of hyperoside (P > 0.05). Conclusion The method is able and sufficient to be used in drug PK studies of hyperoside.展开更多
文摘'Dignity'probably isn’t the first word that comes to mind when you spot a gang of Beijing Pekinese,or京巴(Jingba),pulling their owners down the street.With their snubbed-nose faces,lolling tongues and pattering gait,the ubiquitous lap dog looks as much like an overgrown rodent as it does a prized purebred.But behind their befuddled expressions,these pitiful pups hide an ancient royal lineage.The Pekinese’s original long-haired ancestors were carefully bred to
文摘“狗咬人”虽不是新闻。但是整个故事都围绕着被咬者的耳朵展开。一波三折,让人关注。作者蕴幽默于不动声色的行文中。非常耐读。比如:The dog survived, but the ear didn't. When it finally reached the hospital four hours after being chewed off, it was no longer in any condition to be stitched back on. 动词survived值得读者玩味,而且。When it finally reached the hospital…一句的中的主语it,不是别的而是the ear!我们汉语就不能直译,如译:当耳朵来到医院的时候……】
基金National Nature Science Foundation of China (30572350)New Drug Foundation of State Administration of Traditional Chinese Medicine (DIX005A)
文摘Objective To develop and validate a simple, rapid, sensitive, and reproducible HPLC method for determination of hyperoside in plasma of dogs and for the subsequent pharmacokinetic (PK) study. Methods An accurate and reproducible HPLC-UV method was developed and validated for the determination of hyperoside in plasma of dogs, using kaempferol as internal standard. The plasma samples of dogs following ig administration of hyperoside were analyzed for the detection of quercetin after enzymatic hydrolysis treatment with combined β-glucuronidase and sulphatase. The analytes were separated on a Diamonsil C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-buffer solution (0.1 mol/L NH 4 Ac + 0.3 mmol/L EDTA-Na 2 )-acetic acid (60:40:1) and was delivered at a flow rate of 1 mL/min. The UV detector was set at 370 nm and the column temperature was maintained at 35 ℃. The sample injection volume was 20 μL. Data were collected and analyzed using the ANASTAR software. PK parameters were calculated with DAS software (2.0). Results Linear relationships were validated over the range of 0.01-1 μg/mL for hyperoside (r = 0.9997). The intra- and inter-day precision values for all samples were within 10.0%, and the accuracies of intra- and inter-day assays were within the range of 92.4%-102.4%. The validated method was successfully used to determine the hyperoside concentration in plasma of dogs for up to 12 h, after a single ig administration (25 mg/kg). The mean PK parameters for male and female dogs were as follows: C max (0.18 ± 0.05) and (0.16 ± 0.05) μg/mL, AUC 0-∞ (0.79 ± 0.34) and (0.86 ± 0.27) μg/(mL·h), t 1/2(ka) (0.89 ± 0.41) and (0.88 ± 0.28) h, respectively. Statistical analysis on the PK of hyperoside in male and female groups showed that sex had no significant impact on the PK of hyperoside (P > 0.05). Conclusion The method is able and sufficient to be used in drug PK studies of hyperoside.
