A review of the pangenome:how it affects our understanding of genomic variation,selection and breeding in domestic animals?

As large-scale genomic studies have progressed,it has been revealed that a single reference genome pattern cannot represent genetic diversity at the species level.While domestic animals tend to have complex routes of origin and migration,suggesting a possible omission of some population-specific sequences in the current reference genome.Conversely,the pangenome is a collection of all DNA sequences of a species that contains sequences shared by all individuals(core genome)and is also able to display sequence information unique to each individual(variable genome).The progress of pangenome research in humans,plants and domestic animals has proved that the missing genetic components and the identification of large structural variants(SVs)can be explored through pangenomic studies.Many individual specific sequences have been shown to be related to biological adaptability,phenotype and important economic traits.The maturity of technologies and methods such as third-generation sequencing,Tel-omere-to-telomere genomes,graphic genomes,and reference-free assembly will further promote the development of pangenome.In the future,pangenome combined with long-read data and multi-omics will help to resolve large SVs and their relationship with the main economic traits of interest in domesticated animals,providing better insights into animal domestication,evolution and breeding.In this review,we mainly discuss how pangenome analysis reveals genetic variations in domestic animals(sheep,cattle,pigs,chickens)and their impacts on phenotypes and how this can contribute to the understanding of species diversity.Additionally,we also go through potential issues and the future perspectives of pangenome research in livestock and poultry.

Management practices and technologies for efficient biological sample collection from domestic animals with special reference to Indian field conditions

Management strategies and the use of advanced technologies are equally important for determining the sample number and sampling frame for successful field sampling for animal disease prevalence studies.The quality of the biological samples collected in the field has a direct bearing on the integrity of the data generated,prevalence estimates and subsequent policy decisions on disease control.Hence,compromising the quality of biological samples collected in the field could potentially undermine the priority setting principles in disease control strategies.Biological samples collected from domestic animals in the field are precious materials and require meticulous planning for sample collection,sample storage in the field,transportation,and storage in the laboratory.Poorly managed field sampling has a significant detrimental impact on the sample quality and quantity and directly affects the accuracy of disease prevalence data.A bad choice of sampling tools,containers,storage and transport all have a negative impact on the integrity of the sample and consequently have an impact on the outcome.Over the last two years,as part of our one health animal sampling work in ndia,we have observed challenges and opportunities in the field sampling of animals for disease prevalence studies.This paper aims to provide information on management practices and technologies for efficient biological sample collection from the field and ensure that good quality samples are available fortesting.

Immunoglobulin genes and diversity: what we have learned from domestic animals

This review focuses on the diversity of immunoglobulin （Ig） genes and Ig isotypes that are expressed in domestic animals. Four livestock species--cattle, sheep, pigs, and horses--express a full range of Ig heavy chains （IgHs）, including t J, 6, y, c, and a. Two poultry species （chickens and ducks） express three IgH isotypes, la, u, and a, but not （5. The K and X light chains are both utilized in the four livestock species, but only the X chain is expressed in poultry. V（D）J recombination, somatic hypermutation （SHM）, and gene conversion （GC） are three distinct mechanisms by which immunoglobulin variable region diversity is generated. Different domestic animals may use distinct means to diversifyrearrancled variable reqions of la aenes.

Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals

Background:Schistosomiasis japonica is a common zoonosis.Domestic animals are the primary source of infection and play an important role in disease transmission.The prevalence and infectivity of this disease in domestic animals in China have significantly decreased and,for this reason,diagnostics with a higher sensitivity have become increasingly necessary.It was reported that polymerase chain reaction(PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and specificity.The present study aimed to develop a PCR-based method for detection of Schistosoma japonicum infection in domestic animals.Methods:A specific nested-PCR assay was developed to detect S.japonicum infection in domestic animals via amplification of a 231-bp DNA fragment of retrotransposon SjR2.The developed assay was first used in sera and dry blood filter paper(DBFP)from goats and buffaloes at different time points of infection.Then,78 DBFPs from 39 artificially-infected bovines at 14 and 28 days post-infection and 42 DBFPs from schistosome-negative bovines from the city of Huangshan in the Anhui province were used to evaluate the diagnostic validity.Furthermore,this assay was used to detect S.japonicum infection in domestic animals in Dongzhi and Wangjiang counties.Results:The expected PCR product was detected in eggs and adult worms of S.japonicum and blood samples from S.japonicum-infected goats and water buffaloes,but not from Fasciola and Haemonchus contortus worms.The nested-PCR assay could detect the target S.japonicum DNA in DBFPs from goats and buffaloes after day 3 post-infection.The sensitivity in buffaloes at 14 and 28 days post-infection was 92.30%(36/39)and 100%(39/39),respectively.The specificity was 97.60%(41/42).The positivity rates in Dongzhi and Wangjiang counties were 6.00%and 8.00%in bovines and 22.00%and 16.67%in goats,respectively.The positivity rates in goats in both counties were higher than those in bovines with a significant difference in Dongzhi County but not in Wangjiang County(P<0.05 and P=0.23,respectively).Conclusions:Our results suggest that the developed nested-PCR assay may be used for the diagnosis of S.japonicum infection in domestic animals,and the control of S.japonicum infection in goats should be paid more attention.

Seroprevalence of Toxoplasma gondii infection in domestic animals of Mohmand agency,Pakistan

Objective:To determine the prevalence of toxoplasmosis among domestic animals of Mohmand Agency,Pakistan.Methods:Indirect haemagglutination test was used to detect Toxoplasma gondii(T.gondii)antibodies in the sera.Results:Out of 384 domestic animals,124(32.29%)were seropositive for antibodies of T.gondii.T.gondii antibodies were detected in 56 out of 104 examined goats(53.84%).The seropositivity in male and female goats were 20(38.46%)and 36(69.23%)respectively.The seropositivity of T.gondii in sheep was 36%(n=100)while the prevalence in female sheep was higher(41.66%)as compared to male sheep(30.76%).A total of 8 out of 48 male cows were detected seropositive(16.66%)and 8 out of 32 female cows were detected seropositive(25%).Lowest prevalence was detected in buffaloes which was 16%(n=100).Out of 52 male buffaloes 4 were detected seropositive for T.gondii antibodies(7.69%)while in female buffaloes was 25%(n=48).The seroprevalence of T.gondii antibodies was higher in goats and lower in buffaloes.Conclusions:The findings of the present study indicate that T.gondii infection is high in domestic animals in Mohmand agency,Pakistan,which has implications for public health in this region.Essential control strategies and suitable measures should be carried out rationally in this region,in order to lower the risk of exposure to T.gondii.

The Innovative Expression of Traditional Chinese Cultural Archetypes in Animated Films: Establishing a Reliable, Admirable, and Respectable Image of China

China’s domestic animation industry is deeply rooted in its rich traditional cultural heritage.While continuously exploring and showcasing the unique charm of such cultural heritage through storytelling,imagery establishment,and spirit creation,Chinese animation also seamlessly integrates modern aesthetic characteristics and cultural values into its development within the context of the new era.By adopting a contemporary perspective,it innovatively expresses the essence of traditional culture while striving to shape and present a reliable,admirable and respectable image of China.Chinese animation aims to create credible contemporary animation images by drawing inspiration from traditional Chinese cultural archetypes.It also seeks to revitalize admirable traditional cultural imagery using vibrant and prevailing ACGN visuals,while shaping credible characters with richer cultural connotations and crafting stories with more enchanting plots to convey the spiritual essence of the Chinese nation.By establishing reliable,admirable and respectable animation images,the Chinese animation industry strives to enhance its capability to“tell China’s stories well and make the voice of China heard,”to better promote the image of China in the new era from a global perspective.

Molecular detection of Leishmania species in human and animals from cutaneous leishmaniasis endemic areas of Waziristan, Khyber Pakhtunkhwa, Pakistan

Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.

A Study of the English Translation Strategies of Chinese Film and Television Animation from the Perspective of Skopos Theory

The English translation level of domestic film and television animation not only affects the appreciation effect of English-speaking audiences,but also affects the communication and exchange of Chinese excellent culture in the world,and even affects the commercial interests of film and television production.With the increasing international exchange of various cultures,the traditional theoretical views on translation are no longer suitable for the enrichment and development of modern literary forms.Based on the Skopos Theory,this paper will discuss the translation strategies of film and television animation works and propose the translation methods of film and television animation,to enhance the communication and influence of Chinese culture.

Reimagining safe lithium applications in the living environment and its impacts on human,animal,and plant system

Lithium's(Li)ubiquitous distribution in the environment is a rising concern due to its rapid proliferation in the modern electronic industry.Li enigmatic entry into the terrestrial food chain raises many questions and uncertainties that may pose a grave threat to living biota.We examined the leverage existing published articles regarding advances in global Li resources,interplay with plants,and possible involvement with living organisms,especially humans and animals.Globally,Li concentration(<10 e300 mg kg
1)is detected in agricultural soil,and their pollutant levels vary with space and time.High mobility of Li results in higher accumulation in plants,but the clear mechanisms and specific functions remain unknown.Our assessment reveals the causal relationship between Li level and biota health.For example,lower Li intake(<0.6 mM in serum)leads to mental disorders,while higher intake(>1.5 mM in serum)induces thyroid,stomach,kidney,and reproductive system dysfunctions in humans and animals.However,there is a serious knowledge gap regarding Li regulatory standards in environmental compartments,and mechanistic approaches to unveil its consequences are needed.Furthermore,aggressive efforts are required to define optimum levels of Li for the normal functioning of animals,plants,and humans.This review is designed to revitalize the current status of Li research and identify the key knowledge gaps to fight back against the mountainous challenges of Li during the recent digital revolution.Additionally,we propose pathways to overcome Li problems and develop a strategy for effective,safe,and acceptable applications.