Aim: Recently, there is an increased average of developing cancers. Though, the chemotherapeutic-treatment is unfavorable during pregnancy due to its harmful effects on developing fetuses, physicians have two ways to ...Aim: Recently, there is an increased average of developing cancers. Though, the chemotherapeutic-treatment is unfavorable during pregnancy due to its harmful effects on developing fetuses, physicians have two ways to minimize these effects either by termination of the pregnancy or minimizing its side effects. The present work aimed to illustrate the susceptibility of cardiac, lung and dorsal aorta function to the widely applicable drugs doxorubicin and cisplatin as well as 5-flurouracil. Materials and Methods: Mother albino rats were arranged into four-groups (control, doxorubicin, cisplatin and 5-flurouracil-treated groups). Each pregnant rat received intraperitoneal administration of 0.2 mg/kg body weight at 10th and 14th day of gestation and sacrificed at parturition (two doses). At parturition, serum of mother rats used to assess troponin I, heat shock protein 70, 8-hydroxydeoxyguanosine, vascular endothelial growth factor and adhesion molecules (ICAM-1 & VCAM-1). Isoenzyme electrophoresis of alkaline and acid phosphatases, glucose-6-phosphate dehydrogenase and lactic dehydrogenase were estimated in serum, myocardium and dorsal aorta of mother rats. The myocardium and lung were processed for histopathological investigations for both mothers and their offspring. Single strand (comet assay) and double strand DNA damage were carried out in heart and dorsal aorta of mother rats. Results: The present finding revealed that there are detected alterations of myocardial markers and lung amino acid metabolism as well as disruption of myocardial isoenzymes. DNA damage of myocardium and dorsal aorta were observed. Conclusions: The authors concluded that the metabolic activity of heart and lung is highly susceptible to doxorubicin and cisplatin treatment compared to 5-flurouracil and the therapeutic doses must be degraded.展开更多
Exposure to ethanol during human embryonic period has severe teratogenic effects on the cardiovascular system.In our study,we demonstrated that ethanol of gradient concentra-tions can interfere with the establishment ...Exposure to ethanol during human embryonic period has severe teratogenic effects on the cardiovascular system.In our study,we demonstrated that ethanol of gradient concentra-tions can interfere with the establishment of circulatory system in embryonic zebrafish.The ef-fective concentration to cause 50%malformations(EC_(50))was 182.5 mmol/L.The ethanol pulse exposure experiment displayed that dome stage during embryogenesis is the sensitive time window to ethanol.It is found that 400 mmol/L ethanol pulse exposure can induce circulatory defects in 43%treated embryos.We ruled out the possibility that ethanol can interfere with the process of hematopoiesis in zebrafish.By employing in situ hybridization with endothelial bio-marker(Flk-1),we revealed that ethanol disrupts the establishment of trunk axial vasculature,but has no effect on cranial vessels.Combined with the results of semi-thin histological sections,the in situ hybridization experiments with arterial and venous biomarkers(ephrinB2,ephB4)sug-gested that ethanol mainly interrupts the development of dorsal aorta while has little effect on axial vein.Further study indicated the negative influence of ethanol on the development of hy-pochord in zebrafish.The consequent lack of vasculogenic factors including Radar and Ang-1 partly explains the defects in formation and integrity of dorsal aorta.These results provide im-portant clues to the study of adverse effects of ethanol on the cardiovascular development in human fetus.展开更多
文摘Aim: Recently, there is an increased average of developing cancers. Though, the chemotherapeutic-treatment is unfavorable during pregnancy due to its harmful effects on developing fetuses, physicians have two ways to minimize these effects either by termination of the pregnancy or minimizing its side effects. The present work aimed to illustrate the susceptibility of cardiac, lung and dorsal aorta function to the widely applicable drugs doxorubicin and cisplatin as well as 5-flurouracil. Materials and Methods: Mother albino rats were arranged into four-groups (control, doxorubicin, cisplatin and 5-flurouracil-treated groups). Each pregnant rat received intraperitoneal administration of 0.2 mg/kg body weight at 10th and 14th day of gestation and sacrificed at parturition (two doses). At parturition, serum of mother rats used to assess troponin I, heat shock protein 70, 8-hydroxydeoxyguanosine, vascular endothelial growth factor and adhesion molecules (ICAM-1 & VCAM-1). Isoenzyme electrophoresis of alkaline and acid phosphatases, glucose-6-phosphate dehydrogenase and lactic dehydrogenase were estimated in serum, myocardium and dorsal aorta of mother rats. The myocardium and lung were processed for histopathological investigations for both mothers and their offspring. Single strand (comet assay) and double strand DNA damage were carried out in heart and dorsal aorta of mother rats. Results: The present finding revealed that there are detected alterations of myocardial markers and lung amino acid metabolism as well as disruption of myocardial isoenzymes. DNA damage of myocardium and dorsal aorta were observed. Conclusions: The authors concluded that the metabolic activity of heart and lung is highly susceptible to doxorubicin and cisplatin treatment compared to 5-flurouracil and the therapeutic doses must be degraded.
基金This work was supported by the Nat ional Natural ScienceFoundation of China(Grant No.30328009)National Special Fundfor“211 Project”of China to Fudan University.
文摘Exposure to ethanol during human embryonic period has severe teratogenic effects on the cardiovascular system.In our study,we demonstrated that ethanol of gradient concentra-tions can interfere with the establishment of circulatory system in embryonic zebrafish.The ef-fective concentration to cause 50%malformations(EC_(50))was 182.5 mmol/L.The ethanol pulse exposure experiment displayed that dome stage during embryogenesis is the sensitive time window to ethanol.It is found that 400 mmol/L ethanol pulse exposure can induce circulatory defects in 43%treated embryos.We ruled out the possibility that ethanol can interfere with the process of hematopoiesis in zebrafish.By employing in situ hybridization with endothelial bio-marker(Flk-1),we revealed that ethanol disrupts the establishment of trunk axial vasculature,but has no effect on cranial vessels.Combined with the results of semi-thin histological sections,the in situ hybridization experiments with arterial and venous biomarkers(ephrinB2,ephB4)sug-gested that ethanol mainly interrupts the development of dorsal aorta while has little effect on axial vein.Further study indicated the negative influence of ethanol on the development of hy-pochord in zebrafish.The consequent lack of vasculogenic factors including Radar and Ang-1 partly explains the defects in formation and integrity of dorsal aorta.These results provide im-portant clues to the study of adverse effects of ethanol on the cardiovascular development in human fetus.
