Electrocatalytic hydrogen production from seawater holds enormous promise for clean energy generation.Nevertheless,the direct electrolysis of seawater encounters significant challenges due to poor anodic stability cau...Electrocatalytic hydrogen production from seawater holds enormous promise for clean energy generation.Nevertheless,the direct electrolysis of seawater encounters significant challenges due to poor anodic stability caused by detrimental chlorine chemistry.Herein,we present our recent discovery that the incorporation of Ce into Ni Fe layered double hydroxide nanosheet array on Ni foam(Ce-Ni Fe LDH/NF)emerges as a robust electrocatalyst for seawater oxidation.During the seawater oxidation process,CeO_(2)is generated,effectively repelling Cl^(-)and inhibiting the formation of Cl O-,resulting in a notable enhancement in the oxidation activity and stability of alkaline seawater.The prepared Ce-Ni Fe LDH/NF requires only overpotential of 390 m V to achieve the current density of 1 A cm^(-2),while maintaining long-term stability for 500 h,outperforming the performance of Ni Fe LDH/NF(430 m V,150 h)by a significant margin.This study highlights the effectiveness of a Ce-doping strategy in augmenting the activity and stability of materials based on Ni Fe LDH in seawater electrolysis for oxygen evolution.展开更多
The double perovskite oxides Sr2Mg1-xF exMoO6-δ were investigated as catalysts for the methane oxidation.The structural properties of catalysts were characterized in detail by X-ray diffraction,X-ray photoelectron sp...The double perovskite oxides Sr2Mg1-xF exMoO6-δ were investigated as catalysts for the methane oxidation.The structural properties of catalysts were characterized in detail by X-ray diffraction,X-ray photoelectron spectroscopy and X-ray absorption spectroscopy.The catalytic property was strongly influenced by the Fe substitution.The relation between catalytic performance and the degree of Fe substitution was examined with regard to the structure and surface characteristics of the mixed oxides.The Fe-containing catalysts exhibited higher activity attributable to the possible（Fe2＋,Mo6＋） and （Fe3＋,Mo5＋）valency pairs,and the highest activity was observed for Sr2Mg0.2Fe0.8MoO6-δ.The enhancement of the catalytic activity may be correlated with the Fe-relating surface lattice oxygen species and was discussed in view of the presence of oxygen vacancies.展开更多
The aim of this paper is to investigate the effect of nitrite intercalated Mg-Al layered double hydroxides(Mg-Al LDH-NO_(2))on mortar durability under the coexisting environment of Cl−and SO_(4)^(2-).Cl−and SO_(4)^(2-...The aim of this paper is to investigate the effect of nitrite intercalated Mg-Al layered double hydroxides(Mg-Al LDH-NO_(2))on mortar durability under the coexisting environment of Cl−and SO_(4)^(2-).Cl−and SO_(4)^(2-) binding properties of Mg-Al LDH-NO_(2) in simulated concrete pore solutions,Cl−and SO_(4)^(2-) diffusion properties of mortars with Mg-Al LDHNO 2 were examined.The steel corrosion and resistance of mortar against SO_(4)^(2-) attack were also evaluated.The results indicate that Mg-Al LDH-NO_(2) can effectively adsorb the Cl−and SO_(4)^(2-) in simulated concrete pore solution,and inhibit the diffusion of Cl−and SO_(4)^(2-) into cement mortars.The presence of SO_(4)^(2-) can greatly affect the uptake amount of Cl−,and there is a coupled effect of Cl−and SO_(4)^(2-) on their penetration into mortar specimens.In addition,Mg-Al LDH-NO_(2) can greatly upgrade the resistance of mortars against SO_(4)^(2-) attack and well prevent the steel from corrosion.However,Cl−will aggravate the SO_(4)^(2-) attack and SO_(4)^(2-) can initially decrease and then increase the steel corrosion.展开更多
Mg-Al layered double hydroxide intercalated with CO_(3)^(2-)(CO_(3)·Mg-Al LDH) is effective for treating HCl exhaust gas.HCl reacts with CO_(3)^(2-) in CO_(3)·Mg-Al LDH, resulting in the formation of Cl·...Mg-Al layered double hydroxide intercalated with CO_(3)^(2-)(CO_(3)·Mg-Al LDH) is effective for treating HCl exhaust gas.HCl reacts with CO_(3)^(2-) in CO_(3)·Mg-Al LDH, resulting in the formation of Cl·Mg-Al LDH.We propose that CO_(2) can be used for the desorption of Cl^(-)from Cl·Mg-Al LDH to regenerate CO_(3)·Mg-Al LDH.Herein,we studied the desorption of a from CI-Mg-Al LDH by adding water to Cl·Mg-Al LDH and blowing CO_(2) into it.We also analyzed the effects of temperature and water addition speed on the desorption of CI^(-)from Cl·Mg-Al LDH.Our results show that the added water adhered to CI·Mg-Al LDH and that CO_(2) in the gaseous phase was dissolved in this adhered water,thus generating CO_(3)^(2-).Therefore,anion exchange occurred between CO_(3)^(2-) and Cl^(-)in the Cl·Mg-Al LDH,thus desorbing Cl^(-).展开更多
Rationally manipulating surface reconstruction of catalysts for water oxidation,inducing formation and dynamic accumulation of catalytically active centers still face numerous challenges.Herein,the introduction of[Cr(...Rationally manipulating surface reconstruction of catalysts for water oxidation,inducing formation and dynamic accumulation of catalytically active centers still face numerous challenges.Herein,the introduction of[Cr(C_(2)O_(4))_(3)]^(3-)into NiFe LDHs by intercalation engineering to promote surface reconstruction achieves an advanced oxygen evolution reaction(OER)activity.In view of the weak electronegativity of Cr^(3+) in[Cr(C_(2)O_(4))_(3)]^(3-),the intercalation of[Cr(C_(2)O_(4))_(3)]^(3-)is expected to result in an electron-rich structure of Fe sites in NiFe LDHs,and higher valence state of Ni can be formed with the charge transfer between Fe and Ni.The optimized electronic structure of NiFe-[Cr(C_(2)O_(4))_(3)]^(3-)-LDHs with more active Ni^(3+) species and the expedited dynamic generation of Ni^(3+) (Fe)OOH phase during the OER process contributed to its excellent catalytic property,revealed by in situ X-ray absorption spectroscopy,Raman spectroscopy,and quasi-in situ X-ray photoelectron spectroscopy.With the modulated electronic structure of metal sites,NiFe-[Cr(C_(2)O_(4))_(3)]^(3-)-LDHs exhibited promoted OER property with a lower overpotential of 236 mV at the current density of 10 mA cm^(-2).This work illustrates the intercalation of conjugated anion to dynamically construct desired Ni^(3+) sites with the optimal electronic environment for improved OER electrocatalysis.展开更多
Double - faced iso - object embroidery is carried out byusing the uneven intercalated stitch crafts of the parallel- lines stitch as a main body, being accompanied by oth-er stitch crafts and miscellaneous stitches, d...Double - faced iso - object embroidery is carried out byusing the uneven intercalated stitch crafts of the parallel- lines stitch as a main body, being accompanied by oth-er stitch crafts and miscellaneous stitches, done on thesame textile material. This paper deals with the flower -bird double - faced embroidery fan of the Qing Dynasty,from pattern design, stitching formation and techniqueof color arrangement, describes the possible way of mak-ing double - faced embroidery with iso - object effectboth in the front and in the back.展开更多
Abstract: A new pyrometer, which can solve the affection of emissivity change in temperature measuring, has been developed by double Y - type optical fibers. The mathematical model of the new pyrometer is presented an...Abstract: A new pyrometer, which can solve the affection of emissivity change in temperature measuring, has been developed by double Y - type optical fibers. The mathematical model of the new pyrometer is presented and the errors of the new pyrometer are analyzed.展开更多
The aspect of formation and evolution of the recycled pulsar（PSR J0737-3039 A/B） is investigated, taking into account the contributions of accretion rate, radius and spin-evolution diagram（- diagram） in the double...The aspect of formation and evolution of the recycled pulsar（PSR J0737-3039 A/B） is investigated, taking into account the contributions of accretion rate, radius and spin-evolution diagram（- diagram） in the double pulsar system. Accepting the spin-down age as a rough estimate（or often an upper limit） of the true age of the neutron star, we also impose the restrictions on the radius of this system. We calculate the radius of the recycled pulsar PSR J0737-3039 A ranges approximately from 8.14 to 25.74 km, and the composition of its neutron star nuclear matters is discussed in the mass-radius diagram.展开更多
The inverse problem of wave equation is the importance of study not only in seismic prospecting but also in applied mathematics. With the development of the research, the inverse methods of 1 - D wave equations have b...The inverse problem of wave equation is the importance of study not only in seismic prospecting but also in applied mathematics. With the development of the research, the inverse methods of 1 - D wave equations have been trending towards the multiple parameters inversion . We have obtained an inverse method with double -parameter, in which medium density and wave velocity can be derived simultaneously. In this paper, to increase the inverse accuracy, the method is improved as follows. Firstly, the formula in which the Green Function is omitted are derived and used. Secondly, the regularizing method is reasonable used by choosing the stable function. With the new method, we may derive elastic parameter and medium density or medium density and wave velocity. Thus, lithology parameters for seismic prospecting may be obtained.After comparing the derived values from the new method with that from previous method, we obtain the new method through which substantially improve the derived accuracy . The new method has been applied to real depths inversion for sedimentary strata and volcanic rock strata in Chaoyanggou Terrace of Songliao Basin in eastern China. According to the inverse results,the gas - bearing beds are determlned.展开更多
The thermodynamic instability of zinc anodes in aqueous electrolytes leads to issues such as corrosion,hydrogen evolution reactions(HER), and dendrite growth, severely hindering the practical application of zinc-based...The thermodynamic instability of zinc anodes in aqueous electrolytes leads to issues such as corrosion,hydrogen evolution reactions(HER), and dendrite growth, severely hindering the practical application of zinc-based aqueous energy storage devices. To address these challenges, this work proposes a dualfunction zinc anode protective layer, composed of Zn-Al-In layered double oxides(ILDO) by rationally designing Zn-Al layered double hydroxides(Zn-Al LDHs) for the first time. Differing from previous works on the LDHs coatings, firstly, the ILDO layer accelerates zinc-ion desolvation and also captures and anchors SO_(4)^(2-). Secondly, the in-situ formation of the Zn-In alloy phase effectively lowers the nucleation energy barrier, thereby regulating zinc nucleation. Consequently, the zinc anode with the ILDO protective layer demonstrates long-term stability exceeding 1900 h and low voltage hysteresis of 7.5 m V at 0.5 m A cm^(-2) and 0.5 m A h cm^(-2). Additionally, it significantly enhances the rate capability and cycling performance of Zn@ILDO//MnO_(2) full batteries and Zn@ILDO//activated carbon zinc-ion hybrid capacitors.This simple and effective dual-function protective layer strategy offers a promising approach for achieving high-performance zinc-ion batteries.展开更多
AIM To determine the prevalence and diagnostic value of autoantibodies inα-fetoprotein(AFP)-negative hepatocellular carcinoma(HCC).METHODS Fifty-six serum samples from AFP-negative HCC cases,86 from AFP-positive HCC ...AIM To determine the prevalence and diagnostic value of autoantibodies inα-fetoprotein(AFP)-negative hepatocellular carcinoma(HCC).METHODS Fifty-six serum samples from AFP-negative HCC cases,86 from AFP-positive HCC cases,168 from chronic liver disease cases,and 59 from normal human controls were included in this study.Autoantibodies to nucleophosmin(NPM)1,14-3-3zeta and mouse double minute 2 homolog(MDM2)proteins in AFP-negative HCC serum were evaluated by enzymelinked im munosorbent assay.Partially positive sera were further evaluated by western blotting.Immunohistochemistry was used to detect the expression of three tumor-associated antigens(TAAs)in AFP-negative HCC and normal control tissues.RESULTS The frequency of autoantibodies to the three TAAs in AFP-negative HCC sera was 21.4%,19.6%and 19.6%,which was significantly higher than in the chronic liver disease cases and normal human controls(P<0.01)as well as AFP-positive HCC cases.The sensitivity of the three autoantibodies for diagnosis of AFP-negative HCC ranged from 19.6%to 21.4%,and the specificity was approximately 95%.When the three autoantibodies were combined,the sensitivity reached 30.4%and the specificity reached 91.6%.CONCLUSION Autoantibodies to NPM1,14-3-3zeta and MDM2 may be useful biomarkers for immunodiagnosis of AFP-negative HCC.展开更多
Benzene is an established leukotoxin and leukemogen in humans. We have previously re- ported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein k...Benzene is an established leukotoxin and leukemogen in humans. We have previously re- ported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein kinase catalytic subunit （DNA-PKcs） to mediate the cellular response to DNA double strand break （DSB） caused by DNA-damaging metabolites. In this study, we used a new, small molecule, a selective inhibitor of DNA-PKcs, 2-（morpholin-4-yl）-benzo[h]chomen-4-one （NU7026）, as a probe to analyze the molecular events and pathways in hydroquinone-induced DNA DSB repair and apoptosis. Inhibition of DNA-PKcs by NU7026 markedly potentiated the apoptotic and growth inhibitory effects of hydroquinone in proerythroid leukemic K562 cells in a dose-dependent manner. Treatment with NU7026 did not alter the production of reactive oxygen species and oxidative stress by hydroquinone but repressed the protein level of DNA-PKcs and blocked the induction of the kinase mRNA and protein expression by hydroquinone. Moreover, hydroquinone increased the phos- phorylation of Akt to activate Akt, whereas co-treatment with NU7026 prevented the activation of Akt by hydroquinone. Lastly, hydroquinone and NU7026 exhibited synergistic effects on promoting apop- tosis by increasing the protein levels of pro-apoptotic proteins Bax and caspase-3 but decreasing the protein expression of anti-apoptotic protein Bcl-2. Taken together, the findings reveal a central role of DNA-PKcs in hydroquinone-induced hematotoxicity in which it coordinates DNA DSB repair, cell cycle progression, and apoptosis to regulate the response to hydroquinone-induced DNA damage.展开更多
基金support from the Free Exploration Project of Frontier Technology for Laoshan Laboratory(No.16-02)the National Natural Science Foundation of China(Nos.22072015 and 21927811)。
文摘Electrocatalytic hydrogen production from seawater holds enormous promise for clean energy generation.Nevertheless,the direct electrolysis of seawater encounters significant challenges due to poor anodic stability caused by detrimental chlorine chemistry.Herein,we present our recent discovery that the incorporation of Ce into Ni Fe layered double hydroxide nanosheet array on Ni foam(Ce-Ni Fe LDH/NF)emerges as a robust electrocatalyst for seawater oxidation.During the seawater oxidation process,CeO_(2)is generated,effectively repelling Cl^(-)and inhibiting the formation of Cl O-,resulting in a notable enhancement in the oxidation activity and stability of alkaline seawater.The prepared Ce-Ni Fe LDH/NF requires only overpotential of 390 m V to achieve the current density of 1 A cm^(-2),while maintaining long-term stability for 500 h,outperforming the performance of Ni Fe LDH/NF(430 m V,150 h)by a significant margin.This study highlights the effectiveness of a Ce-doping strategy in augmenting the activity and stability of materials based on Ni Fe LDH in seawater electrolysis for oxygen evolution.
基金the financial supports from the National Natural Science Foundation of China(No.20703042)National Basic Research Program of China(No.2010CB923300)+1 种基金USTC-NSRL Association Funding(No.KY2060030009)the Fundamental Research Funds for the Central Universities
文摘The double perovskite oxides Sr2Mg1-xF exMoO6-δ were investigated as catalysts for the methane oxidation.The structural properties of catalysts were characterized in detail by X-ray diffraction,X-ray photoelectron spectroscopy and X-ray absorption spectroscopy.The catalytic property was strongly influenced by the Fe substitution.The relation between catalytic performance and the degree of Fe substitution was examined with regard to the structure and surface characteristics of the mixed oxides.The Fe-containing catalysts exhibited higher activity attributable to the possible（Fe2＋,Mo6＋） and （Fe3＋,Mo5＋）valency pairs,and the highest activity was observed for Sr2Mg0.2Fe0.8MoO6-δ.The enhancement of the catalytic activity may be correlated with the Fe-relating surface lattice oxygen species and was discussed in view of the presence of oxygen vacancies.
基金Project(51478164)supported by the National Natural Science Foundation of ChinaProject(BK20181306)supported by Natural Science Foundation of Jiangsu Province,China。
文摘The aim of this paper is to investigate the effect of nitrite intercalated Mg-Al layered double hydroxides(Mg-Al LDH-NO_(2))on mortar durability under the coexisting environment of Cl−and SO_(4)^(2-).Cl−and SO_(4)^(2-) binding properties of Mg-Al LDH-NO_(2) in simulated concrete pore solutions,Cl−and SO_(4)^(2-) diffusion properties of mortars with Mg-Al LDHNO 2 were examined.The steel corrosion and resistance of mortar against SO_(4)^(2-) attack were also evaluated.The results indicate that Mg-Al LDH-NO_(2) can effectively adsorb the Cl−and SO_(4)^(2-) in simulated concrete pore solution,and inhibit the diffusion of Cl−and SO_(4)^(2-) into cement mortars.The presence of SO_(4)^(2-) can greatly affect the uptake amount of Cl−,and there is a coupled effect of Cl−and SO_(4)^(2-) on their penetration into mortar specimens.In addition,Mg-Al LDH-NO_(2) can greatly upgrade the resistance of mortars against SO_(4)^(2-) attack and well prevent the steel from corrosion.However,Cl−will aggravate the SO_(4)^(2-) attack and SO_(4)^(2-) can initially decrease and then increase the steel corrosion.
文摘Mg-Al layered double hydroxide intercalated with CO_(3)^(2-)(CO_(3)·Mg-Al LDH) is effective for treating HCl exhaust gas.HCl reacts with CO_(3)^(2-) in CO_(3)·Mg-Al LDH, resulting in the formation of Cl·Mg-Al LDH.We propose that CO_(2) can be used for the desorption of Cl^(-)from Cl·Mg-Al LDH to regenerate CO_(3)·Mg-Al LDH.Herein,we studied the desorption of a from CI-Mg-Al LDH by adding water to Cl·Mg-Al LDH and blowing CO_(2) into it.We also analyzed the effects of temperature and water addition speed on the desorption of CI^(-)from Cl·Mg-Al LDH.Our results show that the added water adhered to CI·Mg-Al LDH and that CO_(2) in the gaseous phase was dissolved in this adhered water,thus generating CO_(3)^(2-).Therefore,anion exchange occurred between CO_(3)^(2-) and Cl^(-)in the Cl·Mg-Al LDH,thus desorbing Cl^(-).
基金support from the National Natural Science Foundation of China(51402100,21905088,21573066 and U19A2017)the Provincial Natural Science Foundation of Hunan(2020JJ5044,2022JJ10006)。
文摘Rationally manipulating surface reconstruction of catalysts for water oxidation,inducing formation and dynamic accumulation of catalytically active centers still face numerous challenges.Herein,the introduction of[Cr(C_(2)O_(4))_(3)]^(3-)into NiFe LDHs by intercalation engineering to promote surface reconstruction achieves an advanced oxygen evolution reaction(OER)activity.In view of the weak electronegativity of Cr^(3+) in[Cr(C_(2)O_(4))_(3)]^(3-),the intercalation of[Cr(C_(2)O_(4))_(3)]^(3-)is expected to result in an electron-rich structure of Fe sites in NiFe LDHs,and higher valence state of Ni can be formed with the charge transfer between Fe and Ni.The optimized electronic structure of NiFe-[Cr(C_(2)O_(4))_(3)]^(3-)-LDHs with more active Ni^(3+) species and the expedited dynamic generation of Ni^(3+) (Fe)OOH phase during the OER process contributed to its excellent catalytic property,revealed by in situ X-ray absorption spectroscopy,Raman spectroscopy,and quasi-in situ X-ray photoelectron spectroscopy.With the modulated electronic structure of metal sites,NiFe-[Cr(C_(2)O_(4))_(3)]^(3-)-LDHs exhibited promoted OER property with a lower overpotential of 236 mV at the current density of 10 mA cm^(-2).This work illustrates the intercalation of conjugated anion to dynamically construct desired Ni^(3+) sites with the optimal electronic environment for improved OER electrocatalysis.
文摘Double - faced iso - object embroidery is carried out byusing the uneven intercalated stitch crafts of the parallel- lines stitch as a main body, being accompanied by oth-er stitch crafts and miscellaneous stitches, done on thesame textile material. This paper deals with the flower -bird double - faced embroidery fan of the Qing Dynasty,from pattern design, stitching formation and techniqueof color arrangement, describes the possible way of mak-ing double - faced embroidery with iso - object effectboth in the front and in the back.
文摘Abstract: A new pyrometer, which can solve the affection of emissivity change in temperature measuring, has been developed by double Y - type optical fibers. The mathematical model of the new pyrometer is presented and the errors of the new pyrometer are analyzed.
基金Supported by the National Program on Key Research and Development Project under Grant No 2016YFA0400801the National Natural Science Foundation of China under Grant Nos 11173034,11673023 and 11364007+2 种基金the Fundamental Research Funds for the Central Universitythe Key Support Disciplines of Theoretical Physics of Guizhou Province Education Bureau under Grant No ZDXK[2015]38the Youth Talents Project of Science and Technology in Education Bureau of Guizhou Province under Grant No KY[2017]204
文摘The aspect of formation and evolution of the recycled pulsar（PSR J0737-3039 A/B） is investigated, taking into account the contributions of accretion rate, radius and spin-evolution diagram（- diagram） in the double pulsar system. Accepting the spin-down age as a rough estimate（or often an upper limit） of the true age of the neutron star, we also impose the restrictions on the radius of this system. We calculate the radius of the recycled pulsar PSR J0737-3039 A ranges approximately from 8.14 to 25.74 km, and the composition of its neutron star nuclear matters is discussed in the mass-radius diagram.
文摘The inverse problem of wave equation is the importance of study not only in seismic prospecting but also in applied mathematics. With the development of the research, the inverse methods of 1 - D wave equations have been trending towards the multiple parameters inversion . We have obtained an inverse method with double -parameter, in which medium density and wave velocity can be derived simultaneously. In this paper, to increase the inverse accuracy, the method is improved as follows. Firstly, the formula in which the Green Function is omitted are derived and used. Secondly, the regularizing method is reasonable used by choosing the stable function. With the new method, we may derive elastic parameter and medium density or medium density and wave velocity. Thus, lithology parameters for seismic prospecting may be obtained.After comparing the derived values from the new method with that from previous method, we obtain the new method through which substantially improve the derived accuracy . The new method has been applied to real depths inversion for sedimentary strata and volcanic rock strata in Chaoyanggou Terrace of Songliao Basin in eastern China. According to the inverse results,the gas - bearing beds are determlned.
基金Natural Science Foundation of Hunan Province (No.2020JJ4734)High Performance Computing Center of Central South University。
文摘The thermodynamic instability of zinc anodes in aqueous electrolytes leads to issues such as corrosion,hydrogen evolution reactions(HER), and dendrite growth, severely hindering the practical application of zinc-based aqueous energy storage devices. To address these challenges, this work proposes a dualfunction zinc anode protective layer, composed of Zn-Al-In layered double oxides(ILDO) by rationally designing Zn-Al layered double hydroxides(Zn-Al LDHs) for the first time. Differing from previous works on the LDHs coatings, firstly, the ILDO layer accelerates zinc-ion desolvation and also captures and anchors SO_(4)^(2-). Secondly, the in-situ formation of the Zn-In alloy phase effectively lowers the nucleation energy barrier, thereby regulating zinc nucleation. Consequently, the zinc anode with the ILDO protective layer demonstrates long-term stability exceeding 1900 h and low voltage hysteresis of 7.5 m V at 0.5 m A cm^(-2) and 0.5 m A h cm^(-2). Additionally, it significantly enhances the rate capability and cycling performance of Zn@ILDO//MnO_(2) full batteries and Zn@ILDO//activated carbon zinc-ion hybrid capacitors.This simple and effective dual-function protective layer strategy offers a promising approach for achieving high-performance zinc-ion batteries.
基金Supported by Clinical Research Cooperation Fund of the Capital Medical University,No.15JL67Project of Science and Technology Development Plan of Beijing Municipal Education Commission,No.KM201610025021+2 种基金High-Tech Personnel Training Program of Beijing Health System,No.2015-3-104Beijing Municipal Science and Technology Commission,No.Z151100004015066Shaanxi Science and Technology Coordination and Innovation Project,No.2016KTZDSF02-02
文摘AIM To determine the prevalence and diagnostic value of autoantibodies inα-fetoprotein(AFP)-negative hepatocellular carcinoma(HCC).METHODS Fifty-six serum samples from AFP-negative HCC cases,86 from AFP-positive HCC cases,168 from chronic liver disease cases,and 59 from normal human controls were included in this study.Autoantibodies to nucleophosmin(NPM)1,14-3-3zeta and mouse double minute 2 homolog(MDM2)proteins in AFP-negative HCC serum were evaluated by enzymelinked im munosorbent assay.Partially positive sera were further evaluated by western blotting.Immunohistochemistry was used to detect the expression of three tumor-associated antigens(TAAs)in AFP-negative HCC and normal control tissues.RESULTS The frequency of autoantibodies to the three TAAs in AFP-negative HCC sera was 21.4%,19.6%and 19.6%,which was significantly higher than in the chronic liver disease cases and normal human controls(P<0.01)as well as AFP-positive HCC cases.The sensitivity of the three autoantibodies for diagnosis of AFP-negative HCC ranged from 19.6%to 21.4%,and the specificity was approximately 95%.When the three autoantibodies were combined,the sensitivity reached 30.4%and the specificity reached 91.6%.CONCLUSION Autoantibodies to NPM1,14-3-3zeta and MDM2 may be useful biomarkers for immunodiagnosis of AFP-negative HCC.
文摘Benzene is an established leukotoxin and leukemogen in humans. We have previously re- ported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein kinase catalytic subunit （DNA-PKcs） to mediate the cellular response to DNA double strand break （DSB） caused by DNA-damaging metabolites. In this study, we used a new, small molecule, a selective inhibitor of DNA-PKcs, 2-（morpholin-4-yl）-benzo[h]chomen-4-one （NU7026）, as a probe to analyze the molecular events and pathways in hydroquinone-induced DNA DSB repair and apoptosis. Inhibition of DNA-PKcs by NU7026 markedly potentiated the apoptotic and growth inhibitory effects of hydroquinone in proerythroid leukemic K562 cells in a dose-dependent manner. Treatment with NU7026 did not alter the production of reactive oxygen species and oxidative stress by hydroquinone but repressed the protein level of DNA-PKcs and blocked the induction of the kinase mRNA and protein expression by hydroquinone. Moreover, hydroquinone increased the phos- phorylation of Akt to activate Akt, whereas co-treatment with NU7026 prevented the activation of Akt by hydroquinone. Lastly, hydroquinone and NU7026 exhibited synergistic effects on promoting apop- tosis by increasing the protein levels of pro-apoptotic proteins Bax and caspase-3 but decreasing the protein expression of anti-apoptotic protein Bcl-2. Taken together, the findings reveal a central role of DNA-PKcs in hydroquinone-induced hematotoxicity in which it coordinates DNA DSB repair, cell cycle progression, and apoptosis to regulate the response to hydroquinone-induced DNA damage.
