The recent development of gene transfer approaches in plants and animals has revealed that transgene can undergo silencing after integration in the genome. Host genes can also be silenced as a consequence of the prese...The recent development of gene transfer approaches in plants and animals has revealed that transgene can undergo silencing after integration in the genome. Host genes can also be silenced as a consequence of the presence of a homologous transgene. More and more investigations have demonstrated that double- stranded RNA can silence genes by triggering degradation of homologous RNA in the cytoplasm and by directing methylation of homologous nuclear DNA sequences. Analyses of Arabidopsis mutants and plant viral suppressors of silencing are unraveling RNA-silencing mechanisms and are assessing the role of methy- lation in transcriptional and posttranscriptional gene silencing. This review will focus on double-stranded RNA mediated mRNA degradation and gene inactivation in plants.展开更多
Objective: To describe the characteristics of short interfering double stranded RNA (dsRNA) against hepatitis C virus (HCV) and to find out the determining factors in design for desirable inhibitory efficacy. Methods:...Objective: To describe the characteristics of short interfering double stranded RNA (dsRNA) against hepatitis C virus (HCV) and to find out the determining factors in design for desirable inhibitory efficacy. Methods: The data were collected and analyzed by retrieval of 229 published short dsRNAs designed for degradation of HCV RNA. Results: Statistical analyses showed that the most frequently involved short dsRNAs were directing against 5′NTR/core and genotype 1b, accounting for 64.2% and 69.9%, respectively. Inhibitory efficacy varied with the structural characteristics of short dsRNAs, of which the most potential were those directed against HCV core region with inhibitory efficacy of 70.2%. Moreover, the mean inhibitory efficacy of short dsRNAs with GC contents from 30% to 52% was higher than that of those with GC contents out of this range. Conclusion: Based on this pooled data in a relatively large sample, the present results provided clues to design for short dsRNAs with more potent inhibitory efficacy.展开更多
文摘The recent development of gene transfer approaches in plants and animals has revealed that transgene can undergo silencing after integration in the genome. Host genes can also be silenced as a consequence of the presence of a homologous transgene. More and more investigations have demonstrated that double- stranded RNA can silence genes by triggering degradation of homologous RNA in the cytoplasm and by directing methylation of homologous nuclear DNA sequences. Analyses of Arabidopsis mutants and plant viral suppressors of silencing are unraveling RNA-silencing mechanisms and are assessing the role of methy- lation in transcriptional and posttranscriptional gene silencing. This review will focus on double-stranded RNA mediated mRNA degradation and gene inactivation in plants.
基金Supported by Grants from the Committee of Science and Technology of Shanghai, China (No.10ZR1413100, and No.114119a1400)
文摘Objective: To describe the characteristics of short interfering double stranded RNA (dsRNA) against hepatitis C virus (HCV) and to find out the determining factors in design for desirable inhibitory efficacy. Methods: The data were collected and analyzed by retrieval of 229 published short dsRNAs designed for degradation of HCV RNA. Results: Statistical analyses showed that the most frequently involved short dsRNAs were directing against 5′NTR/core and genotype 1b, accounting for 64.2% and 69.9%, respectively. Inhibitory efficacy varied with the structural characteristics of short dsRNAs, of which the most potential were those directed against HCV core region with inhibitory efficacy of 70.2%. Moreover, the mean inhibitory efficacy of short dsRNAs with GC contents from 30% to 52% was higher than that of those with GC contents out of this range. Conclusion: Based on this pooled data in a relatively large sample, the present results provided clues to design for short dsRNAs with more potent inhibitory efficacy.
