Objective Doubly labeled water （DLW） method is the gold standard for measuring total energy expenditure （TEE）. We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±...Objective Doubly labeled water （DLW） method is the gold standard for measuring total energy expenditure （TEE）. We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 recruited. TEE was measured by the DLW method, by indirect calorimetry. We also conducted 24-h estimate energy requirements of the subjects. years with body mass index 22.0±1.4 kg/m2 were and basal energy expenditure （BEE） was determined activity, energy balance and factorial approach to Results TEE of subjects by DLW method was 9.45±0.57 M J/day （2258±180 kcal/day）. The 24-h activity was 10.80±0.33 M J/day （2582±136 kcal/day）. The energy requirement, derived from energy balance observations, was 9.93±1.32 M J/day （2373±315 kcal/day）. The BEE of 6.65±0.28 M J/day （1589±67 kcal/day）, calculated by the adjusted Schofield equation, was significantly higher （P〈O.O01） than that measured by indirect calorimetry, 5.99-±0.66 M J/day （1433-±158 kcal/day）. The TEE derived from the factorial approach was 10.31-±0.43 M J/day （2463±104 kcal/day）. Conclusion The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake （RNI）, suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement.展开更多
The purpose of this study was to use the measurement of the PAEE taken from Actiheart in order to calculate the TEE in Costa Rican schoolchildren, and at the same time, to determine the effectiveness when it is compar...The purpose of this study was to use the measurement of the PAEE taken from Actiheart in order to calculate the TEE in Costa Rican schoolchildren, and at the same time, to determine the effectiveness when it is compared against the TEE obtained by the DLW. A total of sixteen male schoolchildren were measured for their total daily energy expenditure (TEE) with the doubly labeled water (DLW) technique. The TEE obtained by the Actiheart monitor was calculated and validated against the DWL. The TEE was obtained adding the physical activity energy expenditure given by the Actiheart, plus the basal metabolic rate, and the energy cost of growth. The Pearson’s product‐moment correlation coefficient and the paired t‐test sample were measured in order to identify the association of the data and to evidence the differences between both measurements of TEE respectively. The Lin’s concordance correlation coefficient and the Bland-Altman plot evaluated the concordance of both methods. The correlation between the TEE obtained by DLW and the one calculated by Actiheart was r = 0.97, P < 0.001. The paired t-test showed no significant differences between both methods. Lin’s concordance correlation coefficient was Cb = 0.99, classified as almost perfect. The study validated the TEE calculated with Actiheart against the TEE measured by the DLW.展开更多
Objective To determine the thermic effect of food(TEF)in a Chinese mixed diet in young people.Methods During the study,the participants were weighed and examined for body composition every morning.The total energy exp...Objective To determine the thermic effect of food(TEF)in a Chinese mixed diet in young people.Methods During the study,the participants were weighed and examined for body composition every morning.The total energy expenditure(TEE)of the participants was measured by the doubly labeled water method for 7 days,and during this period,basal energy expenditure was measured by indirect calorimetry and physical activity energy expenditure was measured by an accelerometer.The value obtained by subtracting basal energy expenditure and physical activity energy expenditure from TEE was used to calculate TEF.Results Twenty healthy young students(18–30 years;10 male)participated in the study.The energy intake of the participants was not significantly different from the Chinese Dietary Reference Intake of energy(P>0.05).The percentage of energy from protein,fat and carbohydrate were all in the normal range.The intakes of fruits,milk and dietary fiber of the participants were significantly lower than those in the Chinese Dietary Guidelines(P<0.05).There was no significant difference in the body weight of the participants during the experiment(P>0.05).When adjusted for body weight,there was no significant difference in either TEE or basal energy expenditure between the male and female participants(P>0.05).In addition,there was no significant difference in physical activity energy expenditure and TEF between the male and female participants(P>0.05).The percentage of TEF in TEE was 8.73%.Conclusion The percentage of TEF in TEE in a Chinese mixed diet in young people was significantly lower than 10%(P<0.001).A value of 10%is usually considered to be the TEF in mixed diets as a percentage of TEE.展开更多
Energy expenditure is a key variable in the study of ageing, and the fruit fly Drosophila melanogaster is a model organism that has been used to make step changes in our understanding of the ageing process. Standard m...Energy expenditure is a key variable in the study of ageing, and the fruit fly Drosophila melanogaster is a model organism that has been used to make step changes in our understanding of the ageing process. Standard methods for measurement of energy expenditure involve placing individuals in metabolic chambers where their oxygen consumption and CO2 production can be quantified. These measurements require separating individuals from any social context, and may only poorly reflect the environment in which the animals normally live. The doubly-labeled water （DLW） method is an isotope-based technique for measuring energy expenditure which overcomes these problems. However, technical challenges mean that the smallest animals this method has been previously applied to weighed 50-200 mg. We overcame these technical challenges to measure energy demands in Drosophila weighing 0.78 mg. Mass-specific energy expenditure varied between 43 and 65 mW·g^-1. These estimates are considerably higher than estimates using indirect calorimetry of Drosophila in small metabolic chambers （around 18 mW·g^-1）. The methodology we have established extends downwards by three orders of magnitude the size of animals that can be measured using DLW. This approach may be of considerable value in future ageing research attempting to understand the genetic and genomic basis of ageing.展开更多
Objective The present study aimed to evaluate the accuracy of the factorial method for estimating energy needs in individuals living in China.Methods Sixteen healthy female adults aged 22.1±1.2 years with a body ...Objective The present study aimed to evaluate the accuracy of the factorial method for estimating energy needs in individuals living in China.Methods Sixteen healthy female adults aged 22.1±1.2 years with a body mass index （kg/m 2） of 20.4±1.7 were selected as subjects.In free-living conditions,energy expenditure （EE） was determined by using the factorial method.At the same time,the doubly labeled water method （DLW） was also used to measure energy expenditure of the subjects and served as the criterion method.EE predicted by the factorial method （EE factorial） was compared with the simultaneous measurement of EE by the validated DLW method （EE DLW）.Results There was excellent agreement between EE factorial （7.46±0.59 MJ/d） and EE DLW （7.64 ± 0.49 MJ/d）,with a difference of-2.6±4.9% （-0.18±0.36 MJ/d）.No significant differences were found between the two methods.EE factorial was highly correlated with EE DLW （r=0.795,P0.001） and a good agreement for individuals was found by using the Bland and Altman test.Conclusion The factorial method gives satisfactory estimates of EE for both groups and individuals living in China.展开更多
Purpose:The purpose of this study was to compare established methods with newly-developed methods for estimating the total energy expenditure(TEE). Methods:The study subjects comprised 46 individuals,including 16 midd...Purpose:The purpose of this study was to compare established methods with newly-developed methods for estimating the total energy expenditure(TEE). Methods:The study subjects comprised 46 individuals,including 16 middle-aged men(mean age 51.4 years),14 middle-aged women(mean age 49.9 years) and 16 young women(mean age 19.1 years).The TEE was estimated from 24-h heart rate(HR) data using newly-developed software (MoveSense HRAnalyzer 201 la,RC1.Suunto Oy,Vantaa,Finland),and was compared against the TEE determined using doubly labeled water (DLW).Agreement between the two methods was analyzed using Bland and Altman plots. Results:The HR method yielded similar TEE values as the DLW method at the group level,with an average of 8.6 kcal/day in the difference in the mean,but with large individual variations.Forty-four(96%) out of 46 subjects fell within±2SD of the mean difference in TEE comparisons, and there was no tendency towards under- or over-estimation. Conclusion:Our results indicate that the current software using HR analysis for the estimation of daily TEE needs further development for use with free-living individuals.展开更多
Purpose: The purpose of the present study was to determine if there were any differences in various aspects of physical activity such as energy expenditure, intensity, and type of activity between normal-weight and ov...Purpose: The purpose of the present study was to determine if there were any differences in various aspects of physical activity such as energy expenditure, intensity, and type of activity between normal-weight and overweight boys.Methods: Children aged 9—12 years were recruited from 2 elementary schools located in different urban districts in Republic of Korea. The present study included 45 Korean boys, of which 19 were normal-weight and 26 were overweight. Daily physical activity was estimated over the same 1-week study period under free-living conditions using the doubly labeled water(DLW) method and a tri-axial accelerometer. Resting metabolic rate(RMR) was measured using the Douglas bag method and open-circuit indirect calorimetry. We calculated the physical activity level(PAL) as the total energy expenditure(TEE)/RMR.Results: PAL was not significantly different between the groups. In the accelerometer data, time spent in locomotive moderate-to-vigorous physical activity(MVPA) was significantly lower in overweight boys than in normal-weight subjects, whereas other variables including non-locomotive activity did not differ between groups. In addition, among all participants, time spent in total locomotive activity was significantly associated with PAL. Time spent in locomotive MVPA was significantly associated with PAL.Conclusion: Overweight boys may be less physically active based on locomotive MVPA, which was positively related with PAL. Our findings suggest that the contribution of locomotive MVPA to the increase in PAL was relatively significant.展开更多
The aim of this study was to gather information on the use of stable isotopes to measure total body water and body composition scan in children. We selected studies in the last 13 years, in addition to classical studi...The aim of this study was to gather information on the use of stable isotopes to measure total body water and body composition scan in children. We selected studies in the last 13 years, in addition to classical studies on the subject, indexed in the database PubMed, LILACS, BVS and SciELO. The body composition was characterized by the amount of bone tissue, muscle and adipose tissue, also including the organs as well as levels of body water. Your knowledge becomes increasingly important in light of the changes that occur in the nutritional status of various types of diseases in frameworks, such as diabetes mellitus, protein energy malnutrition, in cases of obesity and metabolic syndrome. The ability to accurately assess body fat mass especially in children is associated with the importance of effective strategies for prevention and treatment of childhood obesity. Historically, in addition to clinical applications, measurements of total body water were used to determine body composition in nutritional studies. To the knowledge of the body composition, the body water can be measured and used by the ingestion of a dose of labeled water. The measured isotope enrichment is a function of the amount of body water. The method of deuterium is particularly interesting for the assessment of body composition in children, due to its characteristics of collection and analysis.展开更多
基金supported by the projects of National Natural Science Foundation of China (No.30771811)the National Technology Research and Development Program in the Eleventh Five-Year Plan of China(No.2008BAI58B01)
文摘Objective Doubly labeled water （DLW） method is the gold standard for measuring total energy expenditure （TEE）. We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 recruited. TEE was measured by the DLW method, by indirect calorimetry. We also conducted 24-h estimate energy requirements of the subjects. years with body mass index 22.0±1.4 kg/m2 were and basal energy expenditure （BEE） was determined activity, energy balance and factorial approach to Results TEE of subjects by DLW method was 9.45±0.57 M J/day （2258±180 kcal/day）. The 24-h activity was 10.80±0.33 M J/day （2582±136 kcal/day）. The energy requirement, derived from energy balance observations, was 9.93±1.32 M J/day （2373±315 kcal/day）. The BEE of 6.65±0.28 M J/day （1589±67 kcal/day）, calculated by the adjusted Schofield equation, was significantly higher （P〈O.O01） than that measured by indirect calorimetry, 5.99-±0.66 M J/day （1433-±158 kcal/day）. The TEE derived from the factorial approach was 10.31-±0.43 M J/day （2463±104 kcal/day）. Conclusion The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake （RNI）, suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement.
文摘The purpose of this study was to use the measurement of the PAEE taken from Actiheart in order to calculate the TEE in Costa Rican schoolchildren, and at the same time, to determine the effectiveness when it is compared against the TEE obtained by the DLW. A total of sixteen male schoolchildren were measured for their total daily energy expenditure (TEE) with the doubly labeled water (DLW) technique. The TEE obtained by the Actiheart monitor was calculated and validated against the DWL. The TEE was obtained adding the physical activity energy expenditure given by the Actiheart, plus the basal metabolic rate, and the energy cost of growth. The Pearson’s product‐moment correlation coefficient and the paired t‐test sample were measured in order to identify the association of the data and to evidence the differences between both measurements of TEE respectively. The Lin’s concordance correlation coefficient and the Bland-Altman plot evaluated the concordance of both methods. The correlation between the TEE obtained by DLW and the one calculated by Actiheart was r = 0.97, P < 0.001. The paired t-test showed no significant differences between both methods. Lin’s concordance correlation coefficient was Cb = 0.99, classified as almost perfect. The study validated the TEE calculated with Actiheart against the TEE measured by the DLW.
基金supported by Danone Fund for Dietary Nutrition Research and Education[DIC2021-03].
文摘Objective To determine the thermic effect of food(TEF)in a Chinese mixed diet in young people.Methods During the study,the participants were weighed and examined for body composition every morning.The total energy expenditure(TEE)of the participants was measured by the doubly labeled water method for 7 days,and during this period,basal energy expenditure was measured by indirect calorimetry and physical activity energy expenditure was measured by an accelerometer.The value obtained by subtracting basal energy expenditure and physical activity energy expenditure from TEE was used to calculate TEF.Results Twenty healthy young students(18–30 years;10 male)participated in the study.The energy intake of the participants was not significantly different from the Chinese Dietary Reference Intake of energy(P>0.05).The percentage of energy from protein,fat and carbohydrate were all in the normal range.The intakes of fruits,milk and dietary fiber of the participants were significantly lower than those in the Chinese Dietary Guidelines(P<0.05).There was no significant difference in the body weight of the participants during the experiment(P>0.05).When adjusted for body weight,there was no significant difference in either TEE or basal energy expenditure between the male and female participants(P>0.05).In addition,there was no significant difference in physical activity energy expenditure and TEF between the male and female participants(P>0.05).The percentage of TEF in TEE was 8.73%.Conclusion The percentage of TEF in TEE in a Chinese mixed diet in young people was significantly lower than 10%(P<0.001).A value of 10%is usually considered to be the TEF in mixed diets as a percentage of TEE.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB 13030000) a 1000 talents professorship
文摘Energy expenditure is a key variable in the study of ageing, and the fruit fly Drosophila melanogaster is a model organism that has been used to make step changes in our understanding of the ageing process. Standard methods for measurement of energy expenditure involve placing individuals in metabolic chambers where their oxygen consumption and CO2 production can be quantified. These measurements require separating individuals from any social context, and may only poorly reflect the environment in which the animals normally live. The doubly-labeled water （DLW） method is an isotope-based technique for measuring energy expenditure which overcomes these problems. However, technical challenges mean that the smallest animals this method has been previously applied to weighed 50-200 mg. We overcame these technical challenges to measure energy demands in Drosophila weighing 0.78 mg. Mass-specific energy expenditure varied between 43 and 65 mW·g^-1. These estimates are considerably higher than estimates using indirect calorimetry of Drosophila in small metabolic chambers （around 18 mW·g^-1）. The methodology we have established extends downwards by three orders of magnitude the size of animals that can be measured using DLW. This approach may be of considerable value in future ageing research attempting to understand the genetic and genomic basis of ageing.
基金supported by the National Natural Science Foundation of China (No. 30771811)the Scientific Research Foundation of Hebei Medical University for the Young Core Instructor (No. 30900143)
文摘Objective The present study aimed to evaluate the accuracy of the factorial method for estimating energy needs in individuals living in China.Methods Sixteen healthy female adults aged 22.1±1.2 years with a body mass index （kg/m 2） of 20.4±1.7 were selected as subjects.In free-living conditions,energy expenditure （EE） was determined by using the factorial method.At the same time,the doubly labeled water method （DLW） was also used to measure energy expenditure of the subjects and served as the criterion method.EE predicted by the factorial method （EE factorial） was compared with the simultaneous measurement of EE by the validated DLW method （EE DLW）.Results There was excellent agreement between EE factorial （7.46±0.59 MJ/d） and EE DLW （7.64 ± 0.49 MJ/d）,with a difference of-2.6±4.9% （-0.18±0.36 MJ/d）.No significant differences were found between the two methods.EE factorial was highly correlated with EE DLW （r=0.795,P0.001） and a good agreement for individuals was found by using the Bland and Altman test.Conclusion The factorial method gives satisfactory estimates of EE for both groups and individuals living in China.
基金funded by the Academy of Finlandthe Finnish Ministry of Education,Suunto Oy+2 种基金the Shanghai overseas distinguish professor award program 2011the Shanghai Key Lab of Human Performance(No.11DZ2261100)2012 National Science and Technology Infrastructure Program(Grant No. 2012BAK21B00).
文摘Purpose:The purpose of this study was to compare established methods with newly-developed methods for estimating the total energy expenditure(TEE). Methods:The study subjects comprised 46 individuals,including 16 middle-aged men(mean age 51.4 years),14 middle-aged women(mean age 49.9 years) and 16 young women(mean age 19.1 years).The TEE was estimated from 24-h heart rate(HR) data using newly-developed software (MoveSense HRAnalyzer 201 la,RC1.Suunto Oy,Vantaa,Finland),and was compared against the TEE determined using doubly labeled water (DLW).Agreement between the two methods was analyzed using Bland and Altman plots. Results:The HR method yielded similar TEE values as the DLW method at the group level,with an average of 8.6 kcal/day in the difference in the mean,but with large individual variations.Forty-four(96%) out of 46 subjects fell within±2SD of the mean difference in TEE comparisons, and there was no tendency towards under- or over-estimation. Conclusion:Our results indicate that the current software using HR analysis for the estimation of daily TEE needs further development for use with free-living individuals.
基金supported by a National Research Foundation of Korea Grant funded by the Korean Government (NRF2013S1A5A2A03044895 JHP)
文摘Purpose: The purpose of the present study was to determine if there were any differences in various aspects of physical activity such as energy expenditure, intensity, and type of activity between normal-weight and overweight boys.Methods: Children aged 9—12 years were recruited from 2 elementary schools located in different urban districts in Republic of Korea. The present study included 45 Korean boys, of which 19 were normal-weight and 26 were overweight. Daily physical activity was estimated over the same 1-week study period under free-living conditions using the doubly labeled water(DLW) method and a tri-axial accelerometer. Resting metabolic rate(RMR) was measured using the Douglas bag method and open-circuit indirect calorimetry. We calculated the physical activity level(PAL) as the total energy expenditure(TEE)/RMR.Results: PAL was not significantly different between the groups. In the accelerometer data, time spent in locomotive moderate-to-vigorous physical activity(MVPA) was significantly lower in overweight boys than in normal-weight subjects, whereas other variables including non-locomotive activity did not differ between groups. In addition, among all participants, time spent in total locomotive activity was significantly associated with PAL. Time spent in locomotive MVPA was significantly associated with PAL.Conclusion: Overweight boys may be less physically active based on locomotive MVPA, which was positively related with PAL. Our findings suggest that the contribution of locomotive MVPA to the increase in PAL was relatively significant.
基金CAPES Graduate Program in Health Sciences of UFSJ
文摘The aim of this study was to gather information on the use of stable isotopes to measure total body water and body composition scan in children. We selected studies in the last 13 years, in addition to classical studies on the subject, indexed in the database PubMed, LILACS, BVS and SciELO. The body composition was characterized by the amount of bone tissue, muscle and adipose tissue, also including the organs as well as levels of body water. Your knowledge becomes increasingly important in light of the changes that occur in the nutritional status of various types of diseases in frameworks, such as diabetes mellitus, protein energy malnutrition, in cases of obesity and metabolic syndrome. The ability to accurately assess body fat mass especially in children is associated with the importance of effective strategies for prevention and treatment of childhood obesity. Historically, in addition to clinical applications, measurements of total body water were used to determine body composition in nutritional studies. To the knowledge of the body composition, the body water can be measured and used by the ingestion of a dose of labeled water. The measured isotope enrichment is a function of the amount of body water. The method of deuterium is particularly interesting for the assessment of body composition in children, due to its characteristics of collection and analysis.
