To investigate effects of Dragon＇s Blood（DB）,a traditional Chinese medicine,on blood coagulation and NO/iNOS level in myocardium and serum of rats in simulated microgravity for the first time,Sprague Dawley（SD）ra...To investigate effects of Dragon＇s Blood（DB）,a traditional Chinese medicine,on blood coagulation and NO/iNOS level in myocardium and serum of rats in simulated microgravity for the first time,Sprague Dawley（SD）rats were randomly divided into six groups：（a）5-day control group,（b）5-day model group,（c）5-day drug group,（d）21-day control group,（e）21-day model group,and（f）21-day drug group.Blood coagulation and NO/iNOS level in myocardium and serum were examined after 5 and 21 days of simulated microgravity respectively.The results showed that blood of tail-suspended rats was in a hypercoagulable state that could not be converted with time extending.Conversely,DB changed these parameters towards normal level and the curative effects became better when tail-suspension lasted till the 21 st day.NO concentration of both myocardium and serum for two periods all increased markedly and DB could effectively reduce these increases except that of 21-day myocardium NO.Activity of iNOS increased markedly as early as 5 days and became more serious on the 21 st day,while DB showed preventive effect on the 21 st day.Western Blot analysis illustrated that the expression of iNOS in the 5-day model group increased significantly over the 5-day control group and the expression in the 5-day drug group dramatically returned to the normal level.The similar trend was observed on the 21-day groups without notable variances.The findings of this study can serve for the further use of Dragon＇s Blood in space diseases.展开更多
目的:分析负压封闭引流技术联合龙血竭粉在水烫伤足踝创面修复中的应用。方法:选择笔者医院2019年7月-2021年1月收治的90例因足踝部水烫伤的患者作为研究对象,依据不同治疗方法均分为两组,对照组45例患者使用龙血竭粉治疗,观察组45例患...目的:分析负压封闭引流技术联合龙血竭粉在水烫伤足踝创面修复中的应用。方法:选择笔者医院2019年7月-2021年1月收治的90例因足踝部水烫伤的患者作为研究对象,依据不同治疗方法均分为两组,对照组45例患者使用龙血竭粉治疗,观察组45例患者在此基础上采取负压封闭引流技术治疗。比较两组临床疗效、创面无渗液时间、结痂时间、创面愈合时间、美国足踝外科学会(American academy of foot and ankle surgery,AOFAS)踝-后足评分、视觉模拟评分表(Visual analogue scale,VAS)、血浆C反应蛋白(C-reactive protein,CRP)水平、白细胞计数(White blood cell count,WBC)及满意评分情况。结果:对照组患者临床疗效(77.78%)显著低于观察组(93.33%),差异有统计学意义(P<0.05)。观察组创面无渗液时间、结痂时间及创面愈合时间均显著低于对照组,差异有统计学意义(P<0.05)。观察组AOFAS评分显著高于对照组,VAS评分显著低于对照组,差异有统计学意义(P<0.05)。观察组WBC、CRP水平显著低于对照组,差异有统计学意义(P<0.05)。对照组患者满意度(77.78%)显著低于观察组(93.33%),差异有统计学意义(P<0.05)。结论:负压封闭引流技术联合龙血竭粉可缩短踝部水烫伤患者创面愈合时间,缓解患者的疼痛,抑制炎症反应,临床疗效较好。展开更多
基金Supported by the National Natural Science Foundation of China(81202996,81573693)National Key Scientific Apparatus Development of Special Item(2012YQ040140)
文摘To investigate effects of Dragon＇s Blood（DB）,a traditional Chinese medicine,on blood coagulation and NO/iNOS level in myocardium and serum of rats in simulated microgravity for the first time,Sprague Dawley（SD）rats were randomly divided into six groups：（a）5-day control group,（b）5-day model group,（c）5-day drug group,（d）21-day control group,（e）21-day model group,and（f）21-day drug group.Blood coagulation and NO/iNOS level in myocardium and serum were examined after 5 and 21 days of simulated microgravity respectively.The results showed that blood of tail-suspended rats was in a hypercoagulable state that could not be converted with time extending.Conversely,DB changed these parameters towards normal level and the curative effects became better when tail-suspension lasted till the 21 st day.NO concentration of both myocardium and serum for two periods all increased markedly and DB could effectively reduce these increases except that of 21-day myocardium NO.Activity of iNOS increased markedly as early as 5 days and became more serious on the 21 st day,while DB showed preventive effect on the 21 st day.Western Blot analysis illustrated that the expression of iNOS in the 5-day model group increased significantly over the 5-day control group and the expression in the 5-day drug group dramatically returned to the normal level.The similar trend was observed on the 21-day groups without notable variances.The findings of this study can serve for the further use of Dragon＇s Blood in space diseases.
文摘目的:分析负压封闭引流技术联合龙血竭粉在水烫伤足踝创面修复中的应用。方法:选择笔者医院2019年7月-2021年1月收治的90例因足踝部水烫伤的患者作为研究对象,依据不同治疗方法均分为两组,对照组45例患者使用龙血竭粉治疗,观察组45例患者在此基础上采取负压封闭引流技术治疗。比较两组临床疗效、创面无渗液时间、结痂时间、创面愈合时间、美国足踝外科学会(American academy of foot and ankle surgery,AOFAS)踝-后足评分、视觉模拟评分表(Visual analogue scale,VAS)、血浆C反应蛋白(C-reactive protein,CRP)水平、白细胞计数(White blood cell count,WBC)及满意评分情况。结果:对照组患者临床疗效(77.78%)显著低于观察组(93.33%),差异有统计学意义(P<0.05)。观察组创面无渗液时间、结痂时间及创面愈合时间均显著低于对照组,差异有统计学意义(P<0.05)。观察组AOFAS评分显著高于对照组,VAS评分显著低于对照组,差异有统计学意义(P<0.05)。观察组WBC、CRP水平显著低于对照组,差异有统计学意义(P<0.05)。对照组患者满意度(77.78%)显著低于观察组(93.33%),差异有统计学意义(P<0.05)。结论:负压封闭引流技术联合龙血竭粉可缩短踝部水烫伤患者创面愈合时间,缓解患者的疼痛,抑制炎症反应,临床疗效较好。
