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Polyploidy Induction and Morphological Characteristics in the Asiatic Hybrid Lily(Lilium spp.cultivar dazzling)
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作者 Xiang Yixun Chen Mingxuan +5 位作者 Chen Jianguo Yu Zhenyu Zhang Jinzhu Yang Tao Che Daidi Fan Jinping 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第2期24-33,共10页
The Asiatic hybrid lily cultivar Lilium dazzling(family Liliaceae)is a perennial herbaceous bulbous plant with bright yellow flowers of ornamental values.It also shows resistance to diseases and abiotic stress,making ... The Asiatic hybrid lily cultivar Lilium dazzling(family Liliaceae)is a perennial herbaceous bulbous plant with bright yellow flowers of ornamental values.It also shows resistance to diseases and abiotic stress,making it an ideal parent for breeding studies.This study established a sterile culture system using scales of dazzling lily as explants to induce polyploidy.Adventitious buds growing to 1 cm were treated with different concentrations of colchicine(125,250 and 500μmol·L-1),pendimethalin or trifluralin(100,200 and 300μmol·L-1,respectively)for 12,24 and 36 h.Flow cytometry and chromosome counting were used to identify ploidy,and the phenotype of the polyploids was examined by stomatal observation and leaf index determination.With increasing mutagen concentration and exposure time,the mortality and mutagenic rate increased.The optimal treatment group was:300μmol·L-1 trifluralin for 12 h,which resulted in 15.55%mortality rate and 42.22%induction rate.The polyploid plants showed a significant increase in stomata length,leaf length and leaf width,with a decrease in stomatal density compared with the control plants.This study provided a basis for polyploid breeding. 展开更多
关键词 POLYPLOID Asiatic hybrid lily TRIFLURALIN flow cytometry plant morphology
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Transcriptomic and physiological analyses identifying Lanzhou lily(Lilium davidii var.unicolor)drought adaptation strategies 被引量:5
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作者 Wenmei Li Yajun Wang +4 位作者 Heng Ren Zhihong Guo Na Li Chengzheng Zhao Zhongkui Xie 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第1期145-157,共13页
Drought stress is the main limiting plant growth factor in arid and semiarid regions.The Lanzhou lily(Lilium davidii var.unicolor)is the only sweet-tasting lily grown in these regions of China that offers highly edibl... Drought stress is the main limiting plant growth factor in arid and semiarid regions.The Lanzhou lily(Lilium davidii var.unicolor)is the only sweet-tasting lily grown in these regions of China that offers highly edible,medicinal,health,and ornamental value.The Tresor lily is an ornamental flower known for its strong resistance.Plants were grown under three different drought intensity treatments,namely,being watered at intervals of 5,15,and 25 d(either throughout the study or during specific growth stages).We measured the biomass,leaf area,photosynthetic response,chlorophyll content(SPAD value),and osmoregulation of both the Lanzhou lily and the Tresor lily(Lilium‘Tresor’).Additionally,we employed RNA sequencing(RNA-Seq)and qRT-PCR to investigate transcriptomic changes of the Lanzhou lily in response to drought stress.Results showed that under drought stress,the decreasing rate in the Lanzhou lily bulb weight was lower than the corresponding Tresor lily bulb rate;the net photosynthetic rate,transpiration rate,and stomatal conductance of the Lanzhou lily were all higher compared to the Tresor lily;osmoregulation constituents,such as glucose,fructose,sucrose,trehalose,and soluble sugar,in the Lanzhou lily were comparatively higher;PYL,NCED,and ERS genes were significantly expressed in the Lanzhou lily.Under moderate drought,the biosynthesis of flavonoids,circadian rhythms,and the tryptophan metabolism pathway of the Lanzhou lily were all significant.Under severe drought stress,fatty acid elongation,photosynthetic antenna protein,plant hormone signal transduction,flavone and flavonol biosynthesis,and the carotenoid biosynthesis pathway were all significant.The Lanzhou lily adapted to drought stress by coordinating its organs and the unique role of its bulb,regulating photosynthesis,increasing osmolyte content,activating circadian rhythms,signal transduction,fatty acid elongation metabolism,and phenylalanine and flavonoid metabolic pathways,which may collectively be the main adaptation strategy and mechanisms used by the Lanzhou lily under drought stress. 展开更多
关键词 Drought stress Adaptation strategy OSMOLYTES Lanzhou lily Physiological characterization Transcription profiles
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GAMYB transcription factor LoMYB65 from lily plays a vital role in pollen development 被引量:1
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作者 Xinyue Liu Ling He +1 位作者 Ze Wu Nianjun Teng 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期223-238,共16页
Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lili... Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lilies.Gibberellin(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive development.GAMYB is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther development.Here,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in Arabidopsis.Fluorescence quantitative PCR results showed that LoMYB65 was mainly expressed in lily anthers.LoMYB65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf cells.The conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its transactivation.Overexpression of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male sterile.Simultaneously,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen amount.Overexpression of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be dose-dependent.Overall,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in lily.LoMYB65 may provide a useful candidate gene for pollenless breeding of lily. 展开更多
关键词 lily Anther development Pollen pollution GAMYB VIGS
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Effects of intercropping on rhizosphere soil microorganisms and root exudates of Lanzhou lily(Lilium davidii var.unicolor) 被引量:7
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作者 CuiPing Hua YaJun Wang +4 位作者 ZhongKui Xie ZhiHong Guo YuBao Zhang Yang Qiu Le Wang 《Research in Cold and Arid Regions》 CSCD 2018年第2期159-168,共10页
Both yield and quality of Lanzhou lily(Lilium davidii var. unicolor) are seriously affected by continuous cropping. We attempted to understand the effects of intercropping on the obstacles associated with continuous c... Both yield and quality of Lanzhou lily(Lilium davidii var. unicolor) are seriously affected by continuous cropping. We attempted to understand the effects of intercropping on the obstacles associated with continuous cropping of Lanzhou lily(Lilium davidii var. unicolor). The changes of rhizosphere microbial biomass and diversity in interplanting and monoculturing systems were studied by using the Illumina Hi Seq sequencing technique. The contents and composition of lily root exudates were measured by gas chromatography–mass spectrometer(GC–MS). The intercropping results of Lanzhou lily showed:(1) There was no difference in the composition of the rhizosphere soil microbes at the phylum level, but the relative abundance of the microbes decreased; and the relative abundance of harmful fungi such as Fusarium sp. increased. The relative abundance of Pleosporales sp. and other beneficial bacteria were reduced. After OTU(operational taxonomic unit)clustering, there were some beneficial bacteria, such as Chaetomium sp., in the lily rhizosphere soil in the interplanting system that had not existed in the single-cropping system. We did not find harmful bacteria that had existed in the single-cropping systm in the rhizosphere soil of interplanting system. The above results indicated that the changes of relative abundance of soil fungi and bacteria in lily rhizosphere soil was not conducive to improving the ecological structure of rhizosphere soil microbes. At the same time, the microbial composition change is very complex—beneficial and yet inadequate at the same time.(2) Root exudates provide a matrix for the growth of microorganisms. Combined with the detection of root exudates, the decrease in the composition of the root exudates of the lily was probably the reason for the decrease of the relative abundance of microbes after intercropping. At the same time, the decrease of the relative content of phenolic compounds, which inhibit the growth of microorganisms, did not increase the relative content of rhizosphere soil microorganisms. Changes in amino acids and total sugars may be responsible for the growth of Fusarium sp.. The results showed that the intercropping pattern did not noticeably alleviate the obstacle to continuous cropping of Lanzhou lily, and the change of microbial biomass and diversity was even unfavorable. However, the emergence of some beneficial bacteria, the disappearance of harmful fungi, and other changes with intercropping are in favor of alleviation of obstacles to continuous cropping of Lanzhou lily. 展开更多
关键词 continuous CROPPING obstacle LANZHOU lily(Lilium davidii var.unicolor) rhizosphere microbial
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Isolation of LiLFY1 and Its Expression in Lily (Lilium longiflorum Thunb.) 被引量:1
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作者 WANG Ai-ju TANG Jin-fu +1 位作者 ZHAO Xiang-yun ZHU Li-huang 《Agricultural Sciences in China》 CAS CSCD 2008年第9期1077-1083,共7页
LFY family genes play a conserved role in regulating flowering. In this study, the cDNA of LiLFY1 was isolated with the strategy of RT-PCR in combination with RACE from lily (Lilium longiflorum Thunb.). LiLFY1 encod... LFY family genes play a conserved role in regulating flowering. In this study, the cDNA of LiLFY1 was isolated with the strategy of RT-PCR in combination with RACE from lily (Lilium longiflorum Thunb.). LiLFY1 encodes a LFY transcriptional factor. The alignment analysis of the deduced LiLFY1 protein with other known LFY family proteins indicates that LiLFY1 is highly homologous with rice RFL and maize FLL. The result of Southern hybridization showed that there are two copies of LFY family genes in lily. LiLFY1 is expressed in young flower buds and shoot apical meristem (SAM) but not in roots, shoots, mature leaves, and mature floral organs. The cloned LiLFY1 gene may be applied to genetic engineering aiming for regulating the flowering time in lily. 展开更多
关键词 lily (Lilium longiflorum Thunb.) LFY transcriptional factor LiLFY1 EXPRESSION FLOWERING
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Comparative transcriptome and proteome analysis of lily clones inoculated with Fusarium oxysporum f. sp. lilii 被引量:1
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作者 Yiping Zhang Xin Wang +8 位作者 Chunlian Jin Feng Xu Xiumei Yang Yalian Jiang Lifang Zhang Lihua Wang Yan Su Lulin Ma Jihua Wang 《Ornamental Plant Research》 2022年第1期237-246,共10页
Basal bulb rot is the major factor restricting the production of lily,caused by Fusarium oxysporum f.sp.lilii.To systematically analyze the transcriptomic and proteomic responses of lily clones to F.oxysporum,we const... Basal bulb rot is the major factor restricting the production of lily,caused by Fusarium oxysporum f.sp.lilii.To systematically analyze the transcriptomic and proteomic responses of lily clones to F.oxysporum,we constructed six RNA-seq libraries and four iTRAQ proteomic libraries using lily resistant and susceptible clones sampled at 0,24 and 48 h post-inoculation(hpi)with F.oxysporum respectively.137,715 unigenes were generated,of which 7,667 were differentially expressed.1,679 and 4,051 differentially expressed genes(DEGs)between resistant and susceptible clones were isolated from samples collected at 0 and 48 hpi.Four hundred and thirty three and 155 DEGs were identified in resistant clones sampled at 24 and 48 hpi separately while 550 and 799 DEGs were isolated in the susceptible clones sampled at 24 and 48 hpi respectively.The results of iTRAQ analysis revealed 7,482 proteins in resistant and susceptible clones.Data analysis of transcriptome and proteome indicated that 5,735 proteins corresponded to mRNAs.Three hundred and sixteen and 1,052 DEGs had corresponding DEPs.At 48 hpi,the resistant clones showed 155 DEGs and 108 corresponding DEPs,while the susceptible clones showed 799 DEGs and 316 corresponding DEPs.In general,these results enhance comprehension of the defense response of lily resistant clones to F.oxysporum infection and provide valuable sequence data for studying the resistance mechanism. 展开更多
关键词 OXYSPORUM analysis lily
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Genetic Diversity of Five Different Lily(Lilium L.)Species in Lithuania Revealed by ISSR Markers
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作者 Judita Zukauskiene Algimantas Paulauskas +2 位作者 Judita Varkuleviciene Rita Marseliene Vytaute Gliaudelyte 《American Journal of Plant Sciences》 2014年第18期2741-2747,共7页
To study the genetic diversity and structure of lily (Lilium L.), we collected 35 samples from Vytautas Magnus University Kaunas Botanical Garden, and analyzed their mutual Simple Sequence Repeat (ISSR) molecular mark... To study the genetic diversity and structure of lily (Lilium L.), we collected 35 samples from Vytautas Magnus University Kaunas Botanical Garden, and analyzed their mutual Simple Sequence Repeat (ISSR) molecular markers. For genetic analysis of lily we chose the lily 6 markers. ISSR data revealed a relatively high level of genetic diversity at the different levels of the group, with 95% of polymorphic loci, effective number of alleles of 1.21, the average expected heterozygosis of 0.15 and Shannon’s information index of 0.26. ANOVA analysis and UPGMA-dendrogram suggested a hierarchical structure between species. 展开更多
关键词 ISSR lily Species Genetic Variability Lithuania
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Tissue Culture of Calla Lily (Zantedeschia spreng.): An Updated Review on the Present Scenario and Future Prospects
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作者 Xuan Sun Xue Wang +6 位作者 Bijaya Sharma Subedi Yin Jiang Di Wang Rongxin Gou Guojun Zhang Wenting Xu Zunzheng Wei 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第8期2413-2428,共16页
The calla lily(Zantedeschia spreng.)is a bulbousflower native to the tropical regions of Africa.Calla lily has gained significant popularity in the international market owing to its intricate morphology and prolonged fl... The calla lily(Zantedeschia spreng.)is a bulbousflower native to the tropical regions of Africa.Calla lily has gained significant popularity in the international market owing to its intricate morphology and prolonged flowering duration.Despite such advantages,for two sub-groups of calla lily,known as group Zantedeschia and group Aestivae,there are challenges in terms of hybrid production due to the‘plastome-genome incompatibility’there-between.Tissue culture is a fundamental biotechnological tool used in gene editing research,with a focus on disease resistance andflower color in calla lily breeding programs.The present review provides a brief background on the history and development of the calla lily,as well as a comprehensive and critical summary of calla lily tissue culture research.The regeneration pathways for both group Zantedeschia and group Aestivae can be divided into de novo organogenesis and somatic embryogenesis.Both groups are capable of obtaining replants through such means.However,only some species in group Aestivae have been reported to be successful in the somatic embryogenesis pathway.In the present review,special attention was paid to the influence of explant types,plant growth regulators,and culture conditions on both de novo organogenesis and somatic embryogenesis in calla lily tissue culture.Ultimately,future research prospects were determined based on integrated analysis of recent progress in calla lily tissue culture research. 展开更多
关键词 Calla lily tissue culture ORGANOGENESIS somatic embryogenesis
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不同百合食药用功能指标检测分析
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作者 杨迎东 王伟东 +6 位作者 张睿琪 冯秀丽 白一光 杨盼盼 周俐宏 李雪艳 胡新颖 《沈阳农业大学学报》 CAS CSCD 北大核心 2024年第3期276-284,共9页
百合(Lilium spp.)是百合科(Liliaceae)百合属(Lilium L.)多年生球根草本植物的统称,是传统的药食同源植物,含有多糖、甾体皂苷等生物活性物质和淀粉、可溶性糖等营养成分,具有抗癌、抗氧化、抗抑郁、止咳等多种药理功能。受品种少、种... 百合(Lilium spp.)是百合科(Liliaceae)百合属(Lilium L.)多年生球根草本植物的统称,是传统的药食同源植物,含有多糖、甾体皂苷等生物活性物质和淀粉、可溶性糖等营养成分,具有抗癌、抗氧化、抗抑郁、止咳等多种药理功能。受品种少、种源退化、重茬障碍等因素制约,现有食药用百合产能无法满足消费市场日益增长的需求,需要开发新的品种。选择百合原生种、药用百合杂交后代和栽培品种为试验材料,检测百合鳞茎多糖、总皂苷、总酚、黄酮、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等生物活性成分和可溶性总糖、维生素C、总淀粉、游离氨基酸、粗蛋白、总膳食纤维等营养成分含量,以卷丹和兰州百合为对照,开展百合食药用指标检测分析。结果表明:大花卷丹多糖含量达到281.58 mg·g^(-1)Dw,多项指标优于或接近卷丹和兰州百合,具有开发成食药用百合新品种的潜力;卷丹杂交后代辽宁晨曦多糖含量244.97 mg·g^(-1)Dw,粗蛋白含量130.94 mg·g^(-1)Dw,适合作为提取百合多糖和加工高蛋白食品的原料;栽培品种穿梭和伯格菲的淀粉、可溶性糖、膳食纤维含量等单项指标较为突出,适合作为提取单类营养物质的原料;凌源产兰州百合与大连产兰州百合5项指标差异极显著,可见产地环境条件能够对百合品质产生重要影响。 展开更多
关键词 百合 食用 药用 检测 分析
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水肥耦合对黄花菜生长发育及产量的影响
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作者 高晶霞 裴红霞 +2 位作者 张清云 席维娜 谢华 《灌溉排水学报》 CAS CSCD 2024年第S01期5-8,共4页
【目的】研究水肥耦合对黄花菜生长发育及产量的影响。【方法】以黄花菜为研究对象,设置滴灌次数和施肥量二因素试验,研究水肥耦合对黄花菜生长发育及产量的影响。【结果】滴水量相同情况下,不同滴水次数处理黄花菜产量差异显著,Ⅳ、Ⅴ... 【目的】研究水肥耦合对黄花菜生长发育及产量的影响。【方法】以黄花菜为研究对象,设置滴灌次数和施肥量二因素试验,研究水肥耦合对黄花菜生长发育及产量的影响。【结果】滴水量相同情况下,不同滴水次数处理黄花菜产量差异显著,Ⅳ、Ⅴ处理黄花菜农艺性状最佳,产量最大为5983.96~6000 kg/hm^(2);施肥时间相同、施肥量不同情况下,S-Ⅳ、S-Ⅴ处理黄花菜的薹高、花蕾数及花蕾等农艺性状表现最佳。【结论】黄花菜在整个生育期应滴水8~10次,施肥525~600 kg/hm^(2)。 展开更多
关键词 水肥 黄花菜 生长发育 产量
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蚯蚓联合生防细菌Bacillus velezensis改善连作百合土壤细菌群落结构及防治枯萎病的效果
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作者 鲁耀雄 高鹏 +6 位作者 彭福元 李卫东 李静 崔新卫 黄国林 潘素君 王运生 《植物营养与肥料学报》 CAS CSCD 北大核心 2024年第1期147-159,共13页
【目的】从土壤理化性状和微生物群落结构及多样性角度,研究蚯蚓联合生防细菌改善百合连作障碍的机理和效果,为湖南百合产业化种植提供技术措施。【方法】采用盆栽试验方法,在湖南长沙连续种植了两季百合(Lilium lancifolium Thunb.)。... 【目的】从土壤理化性状和微生物群落结构及多样性角度,研究蚯蚓联合生防细菌改善百合连作障碍的机理和效果,为湖南百合产业化种植提供技术措施。【方法】采用盆栽试验方法,在湖南长沙连续种植了两季百合(Lilium lancifolium Thunb.)。供试土壤为红壤,生防菌为Bacillales velezensis YFB3-1菌液(菌体浓度为10^(9) CFU/mL),蚯蚓为赤子爱胜蚓(Eisenia fetida)。试验设单施菌液(T1)、只接种蚯蚓(T2)、同时使用菌液和蚯蚓(T3) 3个处理,以不使用菌剂和蚯蚓为对照(CK)。调查了两茬百合产量和枯萎病病情指数,分析了第二茬百合根际土壤理化性质、细菌群落结构和多样性,以及连作百合产量与根际土壤理化性质、细菌群落和枯萎病的相关性。【结果】与第一茬相比,同一处理的第二茬百合产量都显著下降。两年连作百合产量都以同时使用菌液和蚯蚓(T3)处理为最高,分别为16464、15674 kg/hm^(2),都显著高于同年份其他处理。两年连作百合枯萎病发病率和病情指数都以T3为最低,都显著低于同年份其他处理,T3处理百合枯萎病病情指数分别为18.15(2019年)、25.00 (2020年),并且两年的防治效果(T3)都明显优于单施菌液(T1)和接种蚯蚓(T2)。相比于对照(CK),2019、2020年T3处理百合枯萎病防治效果分别为44.55%、37.66%,分别增产15.05%、14.78%。蚯蚓联合生防菌YFB3-1增加了根际土壤Rhizobiales、Flavobacteriales、Pseudomonadales、Bacillales等有益微生物的种群丰度,蚯蚓与生防菌YFB3-1在抑制连作百合枯萎病方面具有显著的互作效应,因而缓解连作百合产量下降的效果最佳。【结论】在施用芽孢杆菌生防菌剂防治连作百合枯萎病的同时,可以增施有机肥(牛粪)来提高土壤中蚯蚓数量,有效预防百合枯萎病发生,减少连作百合产量损失。 展开更多
关键词 连作百合 蚯蚓 贝莱斯芽孢杆菌 枯萎病 土壤细菌群落结构 土壤结构
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不同无土栽培处理对东方百合‘Siberia’籽球生长的影响
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作者 王伟东 白一光 +4 位作者 胡新颖 李雪艳 周俐宏 张睿琪 杨迎东 《园艺与种苗》 CAS 2024年第2期1-3,29,共4页
东方百合籽球抗逆性差,田间土壤栽培易感染病虫害,种球年生长量小。为提高种球质量,缩短繁育周期,开展籽球无土栽培技术研究,建立东方百合种球无土栽培繁育技术体系。[方法]以东方百合‘Siberia’为试验材料,采用单因素随机区组设计,探... 东方百合籽球抗逆性差,田间土壤栽培易感染病虫害,种球年生长量小。为提高种球质量,缩短繁育周期,开展籽球无土栽培技术研究,建立东方百合种球无土栽培繁育技术体系。[方法]以东方百合‘Siberia’为试验材料,采用单因素随机区组设计,探讨不同栽培基质配方、栽培密度、水溶性肥对百合籽球生长的影响。[结果]东方百合籽球无土栽培最佳基质配方为进口草炭∶珍珠岩=3∶1,最适宜无土栽培密度为100粒/箱,生长期施花无缺水溶性肥2000 mg/L最有利于籽球的生长。[结论]筛选出东方百合籽球无土栽培最佳基质配方、栽培密度、肥料种类和浓度,为东方百合籽球规模化生产提供参考。 展开更多
关键词 东方百合 栽培基质 栽培密度 水溶性肥 种球质量
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百合汁饮料生产工艺研究
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作者 陈雪玲 牛欣欣 孙雪琴 《安徽农业科学》 CAS 2024年第8期166-168,共3页
以新鲜百合鳞茎为原料,辅以浓缩苹果汁、蜂蜜等辅料,经科学加工制成具有独特风味的百合汁饮料,对百合汁饮料的关键生产工艺及配方进行了研究。结果表明:采用0.04%柠檬酸、0.10%异VC-钠、0.005%亚硫酸氢钠组成的复合护色剂可以取得理想... 以新鲜百合鳞茎为原料,辅以浓缩苹果汁、蜂蜜等辅料,经科学加工制成具有独特风味的百合汁饮料,对百合汁饮料的关键生产工艺及配方进行了研究。结果表明:采用0.04%柠檬酸、0.10%异VC-钠、0.005%亚硫酸氢钠组成的复合护色剂可以取得理想的护色效果;采用鲜百合∶浓缩苹果汁5∶1(总用量为6%)、0.05%柠檬酸钠、2.00%蜂蜜、0.04%苹果酸、6.00%蔗糖的原辅料配方,可获得口感醇厚、风味独特的百合汁饮料;采用0.10%CMC-Na、0.08%海藻酸钠、0.20%单甘脂、0.05%蔗糖酯(SE-11)构成的复合稳定剂可获得较理想的稳定效果。 展开更多
关键词 百合 饮料 护色 配方 稳定性
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射干制剂对失眠大鼠自主活动及TLR7/MyD88信号通路的影响
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作者 黄赫 赵文嘉 +7 位作者 赵磊 王若冰 包玉龙 范英兰 张瑜 张洪瀛 甘雨 朱竟赫 《长春中医药大学学报》 2024年第1期34-38,共5页
目的 探研射干制剂对PCPA致失眠大鼠自主活动、脑电波以及脑组织TLR7、MyD88基因表达的影响。方法 随机将70只健康SD大鼠分为空白对照组、模型对照组、射干制剂低剂量组(26 mg·kg^(-1))、射干制剂中剂量组(52 mg·kg^(-1))、... 目的 探研射干制剂对PCPA致失眠大鼠自主活动、脑电波以及脑组织TLR7、MyD88基因表达的影响。方法 随机将70只健康SD大鼠分为空白对照组、模型对照组、射干制剂低剂量组(26 mg·kg^(-1))、射干制剂中剂量组(52 mg·kg^(-1))、射干制剂高剂量组(104 mg·kg^(-1))、朱砂安神丸组(1.62 g·kg^(-1))、地西泮片组(0.9 mg·kg^(-1))。荧光定量PCR检测各组TLR7、My D88基因的相对表达量;采用Etho Vision大小鼠行为学系统记录大鼠自主活动情况;通过无线遥测系统记录各组睡眠时脑电图。结果 与空白组对照比较,模型对照组脑组织TLR7、MyD88相对表达量显著升高(P<0.01);同模型对照组相比,射干制剂低剂量组、射干制剂中剂量组TLR7、MyD88的mRNA表达量均降低(P<0.05),射干制剂高剂量组TLR7、MyD88 mRNA表达量显著降低(P<0.01);与空白对照组相比,PCPA造模后大鼠运动量明显增加,不同剂量射干制剂组、朱砂安神丸组及地西泮片组运动有不同程度的减少,射干制剂高剂量组运动减少较为明显;给药120 min后,与空白对照组相比,模型对照组脑电波中的δ波百分比降低(P<0.05);与模型对照组相比,射干低中剂量组、朱砂安神丸组、地西泮片组δ波百分比均升高(P<0.05)。结论 射干制剂可有效抑制大鼠兴奋性,增加脑电图δ波百分比,改善睡眠,这些作用可能是通过调节脑组织TLR7和MyD88基因的表达来实现。 展开更多
关键词 射干制剂 失眠 脑电波 TOLL样受体7基因 髓样分化因子88基因
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百合对土壤重金属污染生物修复能力研究
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作者 谭聪丽 杨跃中 《环境科学与管理》 CAS 2024年第1期154-157,共4页
研究采用卷丹百合植物修复土壤中的重金属污染。在受污染土壤上进行了自然生长的卷丹百合种植实验,分析土壤以及百合的地上和地下部分的重金属含量。结果表明,卷丹百合对重金属的修复能力按照降解速度排列为Cd>As>Zn>Cu,其中Cd... 研究采用卷丹百合植物修复土壤中的重金属污染。在受污染土壤上进行了自然生长的卷丹百合种植实验,分析土壤以及百合的地上和地下部分的重金属含量。结果表明,卷丹百合对重金属的修复能力按照降解速度排列为Cd>As>Zn>Cu,其中Cd和As对其更为敏感,容易吸收和固定。相关性分析发现,Cu和Cd的含量与富集系数呈不显著正相关,而与转运系数呈不显著负相关。Zn含量与富集系数显著正相关,但与转运系数无显著相关性。土壤中的As含量与富集系数和转运系数之间没有相关性。卷丹百合种植对重金属污染土壤具有显著的生物修复能力,尤其是Cd和As的修复效果更佳。 展开更多
关键词 重金属 百合 生物修复 土壤
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百合知母方结合针刺百会、神庭穴对永久性造口严重抑郁症(肝气郁结型)患者临床影响研究
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作者 何谦 张菊 +1 位作者 罗锦 肖燕玲 《四川中医》 2024年第1期143-147,共5页
目的:观察百合知母方结合针刺百会、神庭穴对永久性造口严重抑郁症(肝气郁结型)患者临床影响。方法:研究纳入永久性造口严重抑郁症(肝气郁结型)患者共计79例,将患者以随机数字表法分成对照组(39例)与观察组(40例),对照组患者给予阿戈美... 目的:观察百合知母方结合针刺百会、神庭穴对永久性造口严重抑郁症(肝气郁结型)患者临床影响。方法:研究纳入永久性造口严重抑郁症(肝气郁结型)患者共计79例,将患者以随机数字表法分成对照组(39例)与观察组(40例),对照组患者给予阿戈美拉汀片药物干预及常规临床干预,观察组在对照组基础上给予患者百合知母方结合针刺百会、神庭穴干预,数据观察:疗效、干预前后患者中医证候(忧郁不畅、胸闷肋胀、失眠多梦、易怒善哭、嗳气频作、善太息等)积分变化、汉密尔顿抑郁量表(HAMD)及抑郁自评量表(SDS)评分变化、血清5-羟色胺(5-HT)及脑源性神经营养因子(BDNF)水平变化、不良反应。结果:观察组有效率(97.50%)比对照组(82.05%)更高,P<0.05;干预前,两组患者中医证候(忧郁不畅、胸闷肋胀、失眠多梦、易怒善哭、嗳气频作、善太息等)积分、HAMD及SDS评分、5-HT及BDNF水平比较,P>0.05,干预后各组患者中医证候(忧郁不畅、胸闷肋胀、失眠多梦、易怒善哭、嗳气频作、善太息等)积分、HAMD及SDS评分、5-HT及BDNF水平均改善,观察组患者干预后中医证候(忧郁不畅、胸闷肋胀、失眠多梦、易怒善哭、嗳气频作、善太息等)积分、HAMD及SDS评分、5-HT及BDNF水平等指标均优于对照组患者,P<0.05;两组均未见不良反应。结论:百合知母方结合针刺百会、神庭穴可较好改善永久性造口严重抑郁症(肝气郁结型)患者临床症状,提升临床疗效,促进患者病情好转,值得应用。 展开更多
关键词 永久性造口 严重抑郁症 肝气郁结型 百合知母方 针刺
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株行距和种植深度对西伯利亚百合生长的影响
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作者 黄淑燕 黄云玲 《浙江农业科学》 2024年第7期1657-1662,共6页
阐明不同株行距及种植深度对西伯利亚百合在栽植阶段生长的影响,总结试验栽植地最适西伯利亚百合生长的最佳株行距及种植深度,从中挖掘提高西伯利亚百合切花品质的关键种植因素,寻求绿色、高效的增产提质方式。以西伯利亚2、3代种球为... 阐明不同株行距及种植深度对西伯利亚百合在栽植阶段生长的影响,总结试验栽植地最适西伯利亚百合生长的最佳株行距及种植深度,从中挖掘提高西伯利亚百合切花品质的关键种植因素,寻求绿色、高效的增产提质方式。以西伯利亚2、3代种球为试验对象,按照标准化生产流程,采用二因素随机区组设计,探讨不同株行距及种植深度对百合鲜切花的花茎高度、花苞大小及花苞数量的影响。除株行距对2代种球的花苞数量影响达5%显著水平外,株行距及种植深度对西伯利亚百合2代和3代种球的其他生长指标影响均达1%极显著差异水平。处理3即15 cm×20 cm的株行距25 cm的种植深度组合于2代和3代种球均为最佳处理方式。处理7即25 cm×30 cm的株行距15 cm的种植深度组合对2代和3代种球的花茎高度和花苞直径的生长最不利,处理4不利于种球花苞数量的生长。种植深度对于2代和3代种球花苞数量增长的影响大于株行距。而花茎高度和花苞直径则有所区别,种植深度对2代种球花茎高度生长的影响大于株行距,3代种球反之;株行距对2代种球花苞直径的影响大于种植深度,3代种球反之。于2、3代种球的花茎高度和花苞直径而言,A1B3即以15 cm×20 cm的株行距25 cm的种植深度组合能够获得良好的生长效果。而A3B3即25 cm×30 cm的株行距25 cm的种植深度的组合对2代种球的花苞数量增加有利,A1B3则利于3代种球花苞数量的增加。合适的种植深度能够使植物的根系得到良好的发育,同时能够有效地吸收土壤中的养分和水分,促进植物生长。种球种植株行距与百合花苞数量、花茎高度及花苞大小都呈显著正相关。 展开更多
关键词 株行距 种植深度 西伯利亚 百合
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LilyTask任务并行环境中基于任务关系的初始任务分配算法 被引量:6
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作者 邸楠 王韬 李晓明 《计算机学报》 EI CSCD 北大核心 2005年第5期892-899,共8页
LilyTask是一个基于任务并行的并行程序设计环境,它引入了任务间关系的概念.任务间会由于这种任务依赖关系而产生等待,为了减少这种等待开销,LilyTask系统在预编译阶段分析这些数据依赖关系,并做出相应的静态任务分配.该文给出在LilyTas... LilyTask是一个基于任务并行的并行程序设计环境,它引入了任务间关系的概念.任务间会由于这种任务依赖关系而产生等待,为了减少这种等待开销,LilyTask系统在预编译阶段分析这些数据依赖关系,并做出相应的静态任务分配.该文给出在LilyTask任务并行环境中的一族新的基于任务关系图的静态任务分配的算法——WCP算法,并在实际测试中与另外两个著名的静态分配算法ETF和MCP算法作了比较,测试结果说明WCP算法在任务计算开销与通信开销不能准确给出的情况下有更好的分配效果. 展开更多
关键词 lily TASK 任务并行 静态任务分配 有向无环图 关键路径
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6-BA和VC对百合(Lily)切花瓶插期间的生理影响 被引量:9
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作者 赵明德 刘雅莉 +1 位作者 王西平 陈红武 《西北农业学报》 CAS CSCD 2003年第2期122-125,共4页
探讨了瓶插期间不同浓度的6-BA和VC处理百合切花延缓衰老的机理。结果发现经不同浓度6-BA和VC处理均延长了百合切花瓶插期寿命,并提高了其品质,其中以90mg/L VC和1.5mg/L 6-BA效果较为显著,表现为膜透性降低,丙二醛(MDA)的产生得以抑制... 探讨了瓶插期间不同浓度的6-BA和VC处理百合切花延缓衰老的机理。结果发现经不同浓度6-BA和VC处理均延长了百合切花瓶插期寿命,并提高了其品质,其中以90mg/L VC和1.5mg/L 6-BA效果较为显著,表现为膜透性降低,丙二醛(MDA)的产生得以抑制,花的保鲜效果优于对照。 展开更多
关键词 6-BA VC 百合 切花 瓶插期间 生理 膜进性
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百合鳞茎营养素与植物化学成分研究进展
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作者 米璐 林玉红 +1 位作者 廖小军 徐贞贞 《食品工业科技》 CAS 北大核心 2024年第14期1-14,共14页
百合是多年生草本植物,兼具观赏、食用和药用价值,其可食用或药用部位一般为鳞茎。近年来,百合鳞茎作为一种特色蔬菜,受到消费者青睐。本文重点从不同品种视角对百合鳞茎进行了分析,首先归纳了碳水化合物(包括活性多糖)、蛋白质及氨基... 百合是多年生草本植物,兼具观赏、食用和药用价值,其可食用或药用部位一般为鳞茎。近年来,百合鳞茎作为一种特色蔬菜,受到消费者青睐。本文重点从不同品种视角对百合鳞茎进行了分析,首先归纳了碳水化合物(包括活性多糖)、蛋白质及氨基酸、脂类、矿物质和维生素等营养素的详细构成,进一步总结了其甾体皂苷(包括螺甾烷醇、异螺甾烷醇、变形螺甾烷醇、呋甾烷醇类)、酚酸类(包括单聚酚酸、酚酸甘油酯和苷类)、黄酮类(包括黄酮醇、黄烷醇、查尔酮、黄烷酮、黄烷酮醇、花色苷类等)和生物碱类(包括异喹啉、吡咯啉、哌啶和甾体糖苷类)等已知植物化学成分的构成及其生物活性,最后对百合在保健食品中的应用进行概括,以期为百合研究及非药用加工利用提供理论依据和研究思路。 展开更多
关键词 百合 百合鳞茎 特色蔬菜 植物化学成分 生物活性 功能性食品
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