Background A deterioration in the meat quality of broilers has attracted much more attention in recent years.L-malic acid(MA)is evidenced to decrease meat drip loss in broilers,but the underlying molecular mechanisms ...Background A deterioration in the meat quality of broilers has attracted much more attention in recent years.L-malic acid(MA)is evidenced to decrease meat drip loss in broilers,but the underlying molecular mechanisms are still unclear.It’s also not sure whether the outputs obtained under experimental conditions can be obtained in a com-mercial condition.Here,we investigated the effects and mechanisms of dietary MA supplementation on chicken meat drip loss at large-scale rearing.Results Results showed that the growth performance and drip loss were improved by MA supplementation.Meat metabolome revealed that L-2-aminoadipic acid,β-aminoisobutyric acid,eicosapentaenoic acid,and nicotinamide,as well as amino acid metabolism pathways connected to the improvements of meat quality by MA addition.The transcriptome analysis further indicated that the effect of MA on drip loss was also related to the proper immune response,evidenced by the enhanced B cell receptor signaling pathway,NF-κB signaling pathway,TNF signaling pathway,and IL-17 signaling pathway.Conclusions We provided evidence that MA decreased chicken meat drip loss under commercial conditions.Metabolome and transcriptome revealed a comprehensive understanding of the underlying mechanisms.Together,MA could be used as a promising dietary supplement for enhancing the water-holding capacity of chicken meat.展开更多
The effect of selenium supplementation in finishing broiler diets on meat quality was studied. A corn soya based diet was supplemented with 0.3 ppm of Se from an organic (Seleno methionine;Se-Met) or inorganic source ...The effect of selenium supplementation in finishing broiler diets on meat quality was studied. A corn soya based diet was supplemented with 0.3 ppm of Se from an organic (Seleno methionine;Se-Met) or inorganic source (sodium selenite;Se-Na) and offered to Rossmale chickens from thirty five to fifty two days old. Body weight and feed consumption were recorded during the experimental period. Immediately post sacrifice liver was removed and weight was recorded. The liver glycogen and lactate content was measured. Carcass yield and abdominal fat were determined. At 24 hours post mortem, pHu, colour L*, a*, b*, H°, C*, and drip loss of the Pectoralis and Gastrocnemius muscles were determined. Se-Met supplementation significantly decreases daily food intake. Se-Na significantly increases liver glycogen and lactate content at 3 minutes post mortem. Se-Met and Se-Na significantly increase pHu as compared to the control diet. Also a Se-Met provoked a darker (lower L*) and a redder (higher a*) and a decreased tone (lower H°) in Pectoralis. Se-Met and Se-Na significantly decrease the drip loss in Pectoralis and Gastrocnemius at 24 hours post mortem. We conclude that selenium supplementation decreases food intake and improves meat quality by improving colour and decreasing drip loss, likely by a modulation of reserve of glycogen in liver. The difference among the organic and inorganic sources is not clear and requires further investigation.展开更多
Evaporation loss from the saturated soil beneath drip irrigation emitters highly influences the irrigation efficiency of drip krigation （D1]. Subsurface drip irrigation （SDI） is one good approach to curb this ineff...Evaporation loss from the saturated soil beneath drip irrigation emitters highly influences the irrigation efficiency of drip krigation （D1]. Subsurface drip irrigation （SDI） is one good approach to curb this inefficiency, but in a new irrigation method, straight tube irrigation （STI）, the irrigation tubes do not need to be buried and thus STI is recommended to increase the irrigation efficiency under normal surface-applied DI. STI consists of only connectors and water-transference tubes that can directly transfer irrigation water from the lateral emitters in the drip line to the root zone of plants. Five-month field experiments were carried out in aeolian sand soil in the forest-belts of the Taklimakan Desert, which have poor water storage capacity, to compare the potential water saving between STI and DI. The preliminary results showed that, compared with DI, STI （1） improved the soil water content in soil depths from 40 to 100 cm under the soil surface; （2） achieved the same irrigation effects in relatively shorter irrigation durations; （3） had very little water loss due to deep seepage; and （4） formed a layer of dry sand about 10 to 30 cm thick immediately below the soil surface, which lessened evaporation loss of soil water beneath the emitters on the soil surface. This demonstrates that STI can maximize the water-saving potential of DI through the reduction of wetted soil perimeters on the soil surface. This is valuable information for water-saving engineering applications and projects with STI in arid and semiarid regions.展开更多
[ Objective] To explore the effects of different proportion of bee pollen on the water holding capacity of pork in Duroc Landrace x Yorkshire growing-finishing pigs in order to determine the optimal proportion. [ Met...[ Objective] To explore the effects of different proportion of bee pollen on the water holding capacity of pork in Duroc Landrace x Yorkshire growing-finishing pigs in order to determine the optimal proportion. [ Method] A total of 80 Duroc x Landrace x Yorkshire growing-finishing pigs, weighing （10.0 ± 1.0） kg, were randomly divided into five groups. The experimental period was 120 d including the 5-day pre-feecling period. The corn-soybean meal was not replaced during the whole period. All pigs had free access to feed and water. The pigs were fasting for 24 h but not prohibited from feed before the beginning. Group I was the control group reared with the diet not supplemented bee pollen. The pigs in group II, III ,IV and V were reared with the diet supplemented bee pollen at the concentration of 1%, 3%, 5% and 7%, respectively. After slaughtering, eye muscle was used for determination of water holding capacity through fast filter paper method, drip loss method, cooking loss method and Na- pole yield determination method. [ Resultl With the increase of the proportion of bee pollen, the water holding capacity of pork was first good and then poor. The addition of bee pollen at the concentration of 5% significantly reduced the drip loss of pork and the water holding capacity which was detected by fast filter paper method, but the cooking loss and Napole yield were not significantly influenced. [ Condmion] The bee pollen can effectively improve the water holding capacity of pork in Duroc x Landrace x Yorkshire growing-finishing pigs, and the optimal proportion is 5%.展开更多
基金This study was funded by the Key Laboratory of Feed and Livestock and Poultry Products Quality&Safety Control,Ministry of Agriculture(2021202201)Reform and Development Project of BAAFS(XMS202322).
文摘Background A deterioration in the meat quality of broilers has attracted much more attention in recent years.L-malic acid(MA)is evidenced to decrease meat drip loss in broilers,but the underlying molecular mechanisms are still unclear.It’s also not sure whether the outputs obtained under experimental conditions can be obtained in a com-mercial condition.Here,we investigated the effects and mechanisms of dietary MA supplementation on chicken meat drip loss at large-scale rearing.Results Results showed that the growth performance and drip loss were improved by MA supplementation.Meat metabolome revealed that L-2-aminoadipic acid,β-aminoisobutyric acid,eicosapentaenoic acid,and nicotinamide,as well as amino acid metabolism pathways connected to the improvements of meat quality by MA addition.The transcriptome analysis further indicated that the effect of MA on drip loss was also related to the proper immune response,evidenced by the enhanced B cell receptor signaling pathway,NF-κB signaling pathway,TNF signaling pathway,and IL-17 signaling pathway.Conclusions We provided evidence that MA decreased chicken meat drip loss under commercial conditions.Metabolome and transcriptome revealed a comprehensive understanding of the underlying mechanisms.Together,MA could be used as a promising dietary supplement for enhancing the water-holding capacity of chicken meat.
文摘The effect of selenium supplementation in finishing broiler diets on meat quality was studied. A corn soya based diet was supplemented with 0.3 ppm of Se from an organic (Seleno methionine;Se-Met) or inorganic source (sodium selenite;Se-Na) and offered to Rossmale chickens from thirty five to fifty two days old. Body weight and feed consumption were recorded during the experimental period. Immediately post sacrifice liver was removed and weight was recorded. The liver glycogen and lactate content was measured. Carcass yield and abdominal fat were determined. At 24 hours post mortem, pHu, colour L*, a*, b*, H°, C*, and drip loss of the Pectoralis and Gastrocnemius muscles were determined. Se-Met supplementation significantly decreases daily food intake. Se-Na significantly increases liver glycogen and lactate content at 3 minutes post mortem. Se-Met and Se-Na significantly increase pHu as compared to the control diet. Also a Se-Met provoked a darker (lower L*) and a redder (higher a*) and a decreased tone (lower H°) in Pectoralis. Se-Met and Se-Na significantly decrease the drip loss in Pectoralis and Gastrocnemius at 24 hours post mortem. We conclude that selenium supplementation decreases food intake and improves meat quality by improving colour and decreasing drip loss, likely by a modulation of reserve of glycogen in liver. The difference among the organic and inorganic sources is not clear and requires further investigation.
基金Tarim Oilfield Corporation of China National Petroleum Corporation for providing funds
文摘Evaporation loss from the saturated soil beneath drip irrigation emitters highly influences the irrigation efficiency of drip krigation （D1]. Subsurface drip irrigation （SDI） is one good approach to curb this inefficiency, but in a new irrigation method, straight tube irrigation （STI）, the irrigation tubes do not need to be buried and thus STI is recommended to increase the irrigation efficiency under normal surface-applied DI. STI consists of only connectors and water-transference tubes that can directly transfer irrigation water from the lateral emitters in the drip line to the root zone of plants. Five-month field experiments were carried out in aeolian sand soil in the forest-belts of the Taklimakan Desert, which have poor water storage capacity, to compare the potential water saving between STI and DI. The preliminary results showed that, compared with DI, STI （1） improved the soil water content in soil depths from 40 to 100 cm under the soil surface; （2） achieved the same irrigation effects in relatively shorter irrigation durations; （3） had very little water loss due to deep seepage; and （4） formed a layer of dry sand about 10 to 30 cm thick immediately below the soil surface, which lessened evaporation loss of soil water beneath the emitters on the soil surface. This demonstrates that STI can maximize the water-saving potential of DI through the reduction of wetted soil perimeters on the soil surface. This is valuable information for water-saving engineering applications and projects with STI in arid and semiarid regions.
文摘[ Objective] To explore the effects of different proportion of bee pollen on the water holding capacity of pork in Duroc Landrace x Yorkshire growing-finishing pigs in order to determine the optimal proportion. [ Method] A total of 80 Duroc x Landrace x Yorkshire growing-finishing pigs, weighing （10.0 ± 1.0） kg, were randomly divided into five groups. The experimental period was 120 d including the 5-day pre-feecling period. The corn-soybean meal was not replaced during the whole period. All pigs had free access to feed and water. The pigs were fasting for 24 h but not prohibited from feed before the beginning. Group I was the control group reared with the diet not supplemented bee pollen. The pigs in group II, III ,IV and V were reared with the diet supplemented bee pollen at the concentration of 1%, 3%, 5% and 7%, respectively. After slaughtering, eye muscle was used for determination of water holding capacity through fast filter paper method, drip loss method, cooking loss method and Na- pole yield determination method. [ Resultl With the increase of the proportion of bee pollen, the water holding capacity of pork was first good and then poor. The addition of bee pollen at the concentration of 5% significantly reduced the drip loss of pork and the water holding capacity which was detected by fast filter paper method, but the cooking loss and Napole yield were not significantly influenced. [ Condmion] The bee pollen can effectively improve the water holding capacity of pork in Duroc x Landrace x Yorkshire growing-finishing pigs, and the optimal proportion is 5%.
