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Increase Data Characters to Construct the Molecular Phylogeny of the <i>Drosophila auraria</i>Species Complex
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作者 Lu Gan Gaodong Li +2 位作者 Wenhao Li Qingtao Zeng Yong Yang 《Open Journal of Genetics》 2017年第1期40-49,共10页
Previous phylogenetic analyses of the auraria species complex have led to conflicting hypotheses concerning their relationship;therefore the addition of new sequence data is necessary to discover the phylogeny of this... Previous phylogenetic analyses of the auraria species complex have led to conflicting hypotheses concerning their relationship;therefore the addition of new sequence data is necessary to discover the phylogeny of this species complex. Here we present new data derived from 22 genes to reconstruct the phylogeny of the auraria species complex. A variety of statistical tests, as well as maximum likelihood mapping analysis, were performed to estimate data quality, suggesting that all genes had a high degree of contribution to resolve the phylogeny. Individual locus was analyzed using maximum likelihood (ML), and the concatenated dataset (21,882 bp) were analyzed using partitioned maximum likelihood (ML) and Bayesian analyses. Separated analysis produced various phylogenetic relationships. Phylogenetic topologies from ML and Bayesian analysis based on concatenated dataset show that D. subauraria was well supported as the first species by separated analysis, concatenated dataset analysis, and some previous analysis, then followed by D. auraria and D. biauraria, D. quadraria and D. triauraria. The close relationships of D. quadraria and D. triauraria were consistent with most previous studies. The phylogenetic position of the D. auraria and D. biauraria will be resolved by more data sets. 展开更多
关键词 drosophila auraria species complex phylogenetic Reconstruction Multiple Genes
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用RAPD标记探讨金色果蝇复合种内的遗传分化 被引量:12
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作者 赵中明 陆剑 戴灼华 《动物学报》 SCIE CAS CSCD 北大核心 2001年第6期625-631,共7页
以随机扩增多态性DNA (RAPD)技术分析了金色果蝇复合种的 5个姊妹种 (Drosophileauraria、D .biauraria、D .triauraria、D .quadraria和D .subauraria)共 12个地理种群的遗传变异。在 4 0种 10碱基随机引物中有 3 0种引物对每个种群都... 以随机扩增多态性DNA (RAPD)技术分析了金色果蝇复合种的 5个姊妹种 (Drosophileauraria、D .biauraria、D .triauraria、D .quadraria和D .subauraria)共 12个地理种群的遗传变异。在 4 0种 10碱基随机引物中有 3 0种引物对每个种群都可获得令人满意的扩增结果。在 161个RAPD标记中有 12 9个呈多态性。以遗传距离为尺度用UPGMA法构建了该复合种的聚类关系图。聚类分析结果表明 :D .subauraria处于聚类关系图的基部 ,为该复合种中最早分化的种类 ;其后才是D .biauraria的分化 ,而D .auraria和D .quadraria的分化相对较晚。此结果不支持以往认为D .quadraria为金色果蝇复合种祖先种的观点。RAPD分子标记为金色果蝇复合种的系统演化的研究提供了新的、更有价值的信息。 展开更多
关键词 果蝇 金色果蝇复合种 rapd标记 系统关系 遗传分化
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