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OPG/RANKL/RANK信号通路在脓毒症相关急性肾损伤小鼠中的研究
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作者 李辉 陈蔚霖 牛新荣 《海南医学院学报》 CAS 北大核心 2024年第3期168-174,共7页
目的:探讨骨保护素(OPG)/核因子⁃κB受体活化因子配体(RANKL)/核因子⁃κB受体激活因子(RANK)信号通路因子在脓毒症相关急性肾损伤(SA⁃AKI)小鼠中的改变及可能的作用,为SA⁃AKI的机制研究探索新的思路。方法:通过盲肠结扎穿孔手术(CLP)建... 目的:探讨骨保护素(OPG)/核因子⁃κB受体活化因子配体(RANKL)/核因子⁃κB受体激活因子(RANK)信号通路因子在脓毒症相关急性肾损伤(SA⁃AKI)小鼠中的改变及可能的作用,为SA⁃AKI的机制研究探索新的思路。方法:通过盲肠结扎穿孔手术(CLP)建立SA⁃AKI模型组(CLP组),对照组为假手术组(Sham组),只行开腹不做盲肠结扎穿孔操作。术后24 h采血并处死留取小鼠肾脏组织,用试剂盒检测血清Scr、BUN水平,酶联免疫测定法(ELISA)检验血清中IL⁃6、TNF⁃α、IL⁃1β水平,苏木精⁃伊红染色(HE)观测肾组织病理学变化,聚合酶链反应(RT⁃qPCR)和蛋白质印迹实验(Western blotting)检验小鼠肾组织中OPG、RANKL、RANK的mRNA和蛋白水平。结果:与Sham组对比,CLP组Scr、BUN、IL⁃6、TNF⁃α、IL⁃1β水平显著升高,差异均具有统计学意义(P<0.05);与Sham组对比,CLP组肾小球充血水肿,部分缺血皱缩,球囊间隙扩大,部分肾小管中可见坏死的上皮细胞,管腔扩大,肾间质水肿,少量炎性细胞浸润;与Sham组相比较,CLP组肾组织中OPG和RANK的mRNA表达显著上调,而RANKL的mRNA表达明显下降,差异均具有统计学意义(P<0.05);与Sham组相比较,CLP组肾脏组织中OPG及RANK的蛋白表达均升高,而RANKL的蛋白表达明显下降,差异均具有统计学意义(P<0.05)。结论:在脓毒症的小鼠模型中OPG及RANK表达升高,RANKL表达下降,表明OPG/RANKL/RANK信号通路可能参与了SA⁃AKI的病理生理过程。 展开更多
关键词 脓毒症 急性肾损伤 OPG/rankL/rank信号通路
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膝关节置换术后患者血清RANKL、sVCAM-1、ESR表达水平及其与预后的相关性
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作者 张文 杨卫兵 《中国骨科临床与基础研究杂志》 2024年第3期183-188,共6页
目的 探讨膝关节置换术后患者血清核因子-κB受体活化因子配基(RANKL)、可溶性血管细胞黏附分子-1(sVCAM-1)、红细胞沉降率(ESR)的表达意义及其与预后的关系。方法 回顾性分析2020年1月至2022年9月安阳市人民医院收治的118例膝关节置换... 目的 探讨膝关节置换术后患者血清核因子-κB受体活化因子配基(RANKL)、可溶性血管细胞黏附分子-1(sVCAM-1)、红细胞沉降率(ESR)的表达意义及其与预后的关系。方法 回顾性分析2020年1月至2022年9月安阳市人民医院收治的118例膝关节置换手术患者的临床资料,根据术后6个月Lysholm评分将患者分为预后不良组(总分<70分,27例)和预后良好组(总分≥70分,91例)。比较两组术后1、3个月血清RANKL、sVCAM-1、ESR表达水平,分析其与Lysholm评分的相关性,评估上述血清学指标联合检测对患者术后预后不良的预测价值。结果 术后1、3个月预后不良组血清RANKL、sVCAM-1、ESR水平高于预后良好组(P <0.05),术后1、3个月血清RANKL、sVCAM-1、ESR水平与Lysholm评分呈负相关(P <0.05),联合预测患者术后预后不良的曲线下面积(AUC)优于各血清指标单独预测(P <0.05)。结论 血清RANKL、sVCAM-1、ESR水平升高可提示膝关节置换术患者预后不良风险的增加,联合检测可作为预测预后不良、判断病情转归的参考依据。 展开更多
关键词 关节成形术 置换 预后 rank配体 血管细胞黏附分子1 血沉
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脱氢胆酸调控OPG/RANK和TRAF3抑制破骨细胞分化 被引量:2
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作者 朱禹潼 张晓楠 +1 位作者 关溪 尚东 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第1期12-16,共5页
目的探讨脱氢胆酸(dehydrocholic acid,DHCA)对破骨细胞(osteoclasts,OCs)分化及功能的影响。方法采用粒细胞-巨噬细胞集落刺激因子和核因子κB受体活化因子配体(receptor activator of nuclear factor kappa B(NF-κB)-ligand,RANKL)... 目的探讨脱氢胆酸(dehydrocholic acid,DHCA)对破骨细胞(osteoclasts,OCs)分化及功能的影响。方法采用粒细胞-巨噬细胞集落刺激因子和核因子κB受体活化因子配体(receptor activator of nuclear factor kappa B(NF-κB)-ligand,RANKL)诱导成熟骨髓来源的巨噬细胞分化为OCs。通过抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP/ACP5)染色,确定DHCA抑制OCs形成的最佳浓度。qRT-PCR检测OCs分化和功能相关基因TRAP/ACP5、组织蛋白酶K(cathepsin K,CTSK)和基质金属蛋白酶9(matrix metalloproteinase 9,MMP9)的基因表达。Western blot检测OCs中TNF受体相关因子3(TNF receptor-associated factor 3,TRAF3)、NF-κB受体活化因子(receptor activator of NF-κB,RANK)和骨保护素(osteoprotegerin,OPG)的蛋白水平。结果DHCA浓度为200μmol/L是抑制OCs形成的最佳剂量(P<0.01)。DHCA抑制OCs分化和功能相关基因ACP5、CTSK和MMP9的表达(P<0.01)。DHCA通过下调RANK蛋白和增加TRAF3和OPG蛋白的表达来抑制OCs的分化(P<0.01)。结论DHCA通过调控OPG/RANK和TRAF3抑制OCs分化,这可能是一种有效的骨质疏松前体药物。 展开更多
关键词 脱氢胆酸 破骨细胞 OPG/rank TRAF3 骨质疏松
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基于RANKL/RANK/OPG信号轴探讨骨痹通消颗粒对激素性股骨头坏死模型小鼠的治疗作用
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作者 方祥 周正新 +3 位作者 朱磊 芮仞 许茂玉 朱彩玉 《中国现代医学杂志》 CAS 2024年第7期27-33,共7页
目的观察骨痹通消颗粒对激素性股骨头坏死模型小鼠的治疗作用,并从核因子-κB受体活化因子配体/核因子-κB受体活化因子/骨保护素(RANKL/RANK/OPG)信号轴探讨其作用机制。方法采取腹腔注射脂多糖和臀肌注射醋酸泼尼松龙复制激素性股骨... 目的观察骨痹通消颗粒对激素性股骨头坏死模型小鼠的治疗作用,并从核因子-κB受体活化因子配体/核因子-κB受体活化因子/骨保护素(RANKL/RANK/OPG)信号轴探讨其作用机制。方法采取腹腔注射脂多糖和臀肌注射醋酸泼尼松龙复制激素性股骨头缺血坏死(SONFH)动物模型,经核磁共振鉴定后,将模型复制成功的60只小鼠分成模型(MC)组、骨痹通消颗粒(GBTX)组及通络生骨胶囊(PC)组,每组20只,另设12只正常小鼠作为空白对照(NC)组。分别予以对应组灌胃相应药物12周后麻醉状态下取材,观察各组小鼠治疗前后的一般行为;酶联免疫吸附试验(ELISA)检测各组小鼠的骨碱性磷酸酶(BALP)和I型氨基端延长肽(PINP)含量;Western blotting和实时荧光定量聚合酶链反应(qRT-qPCR)检测各组RANK、RANKL、OPG、磷脂酰肌醇特异性磷脂酶Cγ2(PLCγ2)、组织蛋白酶K(CTSK)、抗酒石酸酸性磷酸酶(TRAP)的蛋白和基因表达。结果核磁共振显示,模型复制成功后的小鼠左侧髋部呈高信号状态。与NC组比较,MC组BALP、PINP水平,以及OPG蛋白和基因相对表达量均降低(P<0.05),RANK、RANKL、PLCγ2、CTSK、TRAP蛋白和基因相对表达量均升高(P<0.05);与MC组比较,GBTX组和PC组BALP、PINP水平,以及OPG蛋白和基因相对表达量均升高(P<0.05),RANK、RANKL、PLCγ2、CTSK、TRAP蛋白和基因相对表达量均下降(P<0.05);与GBTX组比较,PC组OPG、RANK、RANKL、CTSK、TRAP蛋白和基因相对表达量,以及PLCγ2蛋白相对表达量差异均无统计学意义(P>0.05),而PC组PLCγ2基因相对表达量较GBTX组下降(P<0.05)。结论骨痹通消颗粒可能通过上调OPG表达,抑制RANK、RANKL、PLCγ2、CTSK和TRAP表达,从而改善股骨头坏死情况。 展开更多
关键词 激素性股骨头坏死 骨痹通消颗粒 rankL/rank/OPG信号轴
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miR-34a-5p、OPG、RANK、RANKL在肌少-骨质疏松症患者中的作用机制研究
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作者 杨靖元 杨物鹏 +1 位作者 刘昊 王永江 《中文科技期刊数据库(引文版)医药卫生》 2024年第10期0096-0102,共7页
探究miR-34a-5p和OPG/RANK/RANKL在肌少症及骨质疏松中的作用与机制。方法 选取80名患者分为2组,实验组44人肌少-骨质疏松症患者,对照组36人为仅患有骨质疏松症患者。检测血清OPG、RANK、RANKL表达及miR-34a基因含量,采用Excel 2019和SP... 探究miR-34a-5p和OPG/RANK/RANKL在肌少症及骨质疏松中的作用与机制。方法 选取80名患者分为2组,实验组44人肌少-骨质疏松症患者,对照组36人为仅患有骨质疏松症患者。检测血清OPG、RANK、RANKL表达及miR-34a基因含量,采用Excel 2019和SPSS 25.0软件进行数据整理和统计学分析。结果 (1)80名患者中,男性30名,女性50名,性别x2=1.458,年龄年龄x2=-1.225,骨质疏松分为骨质疏松和严重骨质疏松症,骨质疏松症患者p<0.05。(2)实验组和对照组中miR-34a均表达上调,但差异无统计学意义。(3)实验组中miR-34a与OPG、RANK、RANKL表达均有差异,与OPG成正相关,与RANKL成负相关。(4)对照组中miR-34a与OPG、RANK、RANKL表达均有差异,与OPG成正相关,与RANKL成负相关。(5)OPG在对照组中表达上调,RANKL在实验组中表达增加,RANK在两组中表达有差异。结论 (1)miR-34a可能不占主导地位,不能影响肌少症发生,但可通过OPG/RANK/RANKL信号通路影响骨质疏松症。(2)OPG/RANK/RANKL信号通路参与调节骨质疏松症及肌少症罹患过程。 展开更多
关键词 骨质疏松症 肌少症 信号通路 MICRORNA OPG rank rankL
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Er,Cr:YSGG激光治疗对高糖环境下微螺钉周围炎症及对RANK/RANKL信号通路的影响
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作者 刘阳 牛家慧 《口腔颌面修复学杂志》 2024年第3期170-174,共5页
目的:探讨铒铬铱钪镓石榴石(Er,Cr:YSGG)激光治疗对高糖环境下微螺钉周围炎症治疗的疗效,并分析与RANK/RANKL信号通路的关系。方法:20只正常健康雄性新西兰兔随机分为A组(微螺钉周健康组)、B组(微螺钉周围炎症组)、C组(微螺钉周围炎症... 目的:探讨铒铬铱钪镓石榴石(Er,Cr:YSGG)激光治疗对高糖环境下微螺钉周围炎症治疗的疗效,并分析与RANK/RANKL信号通路的关系。方法:20只正常健康雄性新西兰兔随机分为A组(微螺钉周健康组)、B组(微螺钉周围炎症组)、C组(微螺钉周围炎症激光治疗组),皮下注射2%四氧嘧啶建立糖尿病模型,下颌双侧无牙区植入微螺钉种植体,3个月后,A组进行菌斑控制,B组、C组用丝线拴结于微螺钉种植体颈部基台处引入炎症,C组予以Er,Cr:YSGG激光治疗。记录菌斑指数、探诊深度、牙龈指数和龈沟液量;ELISA检测龈沟液炎性细胞白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平;CBCT检查微螺钉种植体周围炎周围骨吸收状态;Western blot检测牙龈组织中核因子κB(NF-κB)/NF-κB受体性活化因子(RANK)/NF-κB受体活化因子配体(RANKL)通路相关蛋白表达。结果:CBCT检查显示与A组比较,B组微螺钉种植体周围牙槽骨有明显的炎性吸收,微螺钉种植体-骨界面愈合差,骨附着不足;与B组比较,C组一侧牙槽骨有少量新生纤维成骨。与A组比较,B组PI、PD、GI水平较高,龈沟液量较多,龈沟液中IL-1β、TNF-α和IL-6水平较高,牙龈中NF-κBp65、RANK、RANKL蛋白表达水平较高(P<0.05);与B组比较,C组PI、PD、GI水平较低,龈沟液量较少,龈沟液中IL-1β、TNF-α和IL-6水平较低,牙龈中NF-κBp65、RANK、RANKL蛋白表达水平较低(P<0.05)。结论:Er,Cr:YSGG激光治疗糖尿病兔颌骨微螺钉种植体周围炎的疗效显著,且在NF-κB/RANK/RANKL通路表达调控上可能发挥一定作用。 展开更多
关键词 铒铬铱钪镓石榴石激光 糖尿病 微螺钉周围炎 核因子κB(NF-κB)/NF-κB受体性活化因子(rank)/NF-κB受体活化因子配体(rankL)通路
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Low rank optimization for efficient deep learning:making a balance between compact architecture and fast training
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作者 OU Xinwei CHEN Zhangxin +1 位作者 ZHU Ce LIU Yipeng 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第3期509-531,F0002,共24页
Deep neural networks(DNNs)have achieved great success in many data processing applications.However,high computational complexity and storage cost make deep learning difficult to be used on resource-constrained devices... Deep neural networks(DNNs)have achieved great success in many data processing applications.However,high computational complexity and storage cost make deep learning difficult to be used on resource-constrained devices,and it is not environmental-friendly with much power cost.In this paper,we focus on low-rank optimization for efficient deep learning techniques.In the space domain,DNNs are compressed by low rank approximation of the network parameters,which directly reduces the storage requirement with a smaller number of network parameters.In the time domain,the network parameters can be trained in a few subspaces,which enables efficient training for fast convergence.The model compression in the spatial domain is summarized into three categories as pre-train,pre-set,and compression-aware methods,respectively.With a series of integrable techniques discussed,such as sparse pruning,quantization,and entropy coding,we can ensemble them in an integration framework with lower computational complexity and storage.In addition to summary of recent technical advances,we have two findings for motivating future works.One is that the effective rank,derived from the Shannon entropy of the normalized singular values,outperforms other conventional sparse measures such as the?_1 norm for network compression.The other is a spatial and temporal balance for tensorized neural networks.For accelerating the training of tensorized neural networks,it is crucial to leverage redundancy for both model compression and subspace training. 展开更多
关键词 model compression subspace training effective rank low rank tensor optimization efficient deep learning
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Ranking academic institutions based on the productivity,impact,and quality of institutional scholars
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作者 Amir Faghri Theodore L.Bergman 《Journal of Data and Information Science》 CSCD 2024年第3期116-154,共39页
Purpose:The quantitative rankings of over 55,000 institutions and their institutional programs are based on the individual rankings of approximately 30 million scholars determined by their productivity,impact,and qual... Purpose:The quantitative rankings of over 55,000 institutions and their institutional programs are based on the individual rankings of approximately 30 million scholars determined by their productivity,impact,and quality.Design/methodology/approach:The institutional ranking process developed here considers all institutions in all countries and regions,thereby including those that are established,as well as those that are emerging in scholarly prowess.Rankings of individual scholars worldwide are first generated using the recently introduced,fully indexed ScholarGPS database.The rankings of individual scholars are extended here to determine the lifetime and last-five-year Top 20 rankings of academic institutions over all Fields of scholarly endeavor,in 14 individual Fields,in 177 Disciplines,and in approximately 350,000 unique Specialties.Rankings associated with five specific Fields(Medicine,Engineering&Computer Science,Life Sciences,Physical Sciences&Mathematics,and Social Sciences),and in two Disciplines(Chemistry,and Electrical&Computer Engineering)are presented as examples,and changes in the rankings over time are discussed.Findings:For the Fields considered here,the Top 20 institutional rankings in Medicine have undergone the least change(lifetime versus last five years),while the rankings in Engineering&Computer Science have exhibited significant change.The evolution of institutional rankings over time is largely attributed to the recent emergence of Chinese academic institutions,although this emergence is shown to be highly Field-and Discipline-dependent.Practical implementations:Existing rankings of academic institutions have:(i)often been restricted to pre-selected institutions,clouding the potential discovery of scholarly activity in emerging institutions and countries;(ii)considered only broad areas of research,limiting the ability of university leadership to act on the assessments in a concrete manner,or in contrast;(iii)have considered only a narrow area of research for comparison,diminishing the broader applicability and impact of the assessment.In general,existing institutional rankings depend on which institutions are included in the ranking process,which areas of research are considered,the breadth(or granularity)of the research areas of interest,and the methodologies used to define and quantify research performance.In contrast,the methods presented here can provide important data over a broad range of granularity to allow responsible individuals to gauge the performance of any institution from the Overall(all Fields)level,to the level of the Specialty.The methods may also assist identification of the root causes of shifts in institution rankings,and how these shifts vary across hundreds of thousands of Fields,Disciplines,and Specialties of scholarly endeavor.Originality/value:This study provides the first ranking of all academic institutions worldwide over Fields,Disciplines,and Specialties based on a unique methodology that quantifies the productivity,impact,and quality of individual scholars. 展开更多
关键词 Academic institution ranking Top institutional scholars ScholarGPS
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RPL-Based IoT Networks under Decreased Rank Attack:Performance Analysis in Static and Mobile Environments
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作者 Amal Hkiri Mouna Karmani +3 位作者 Omar Ben Bahri Ahmed Mohammed Murayr Fawaz Hassan Alasmari Mohsen Machhout 《Computers, Materials & Continua》 SCIE EI 2024年第1期227-247,共21页
The RPL(IPv6 Routing Protocol for Low-Power and Lossy Networks)protocol is essential for efficient communi-cation within the Internet of Things(IoT)ecosystem.Despite its significance,RPL’s susceptibility to attacks r... The RPL(IPv6 Routing Protocol for Low-Power and Lossy Networks)protocol is essential for efficient communi-cation within the Internet of Things(IoT)ecosystem.Despite its significance,RPL’s susceptibility to attacks remains a concern.This paper presents a comprehensive simulation-based analysis of the RPL protocol’s vulnerability to the decreased rank attack in both static andmobilenetwork environments.We employ the Random Direction Mobility Model(RDM)for mobile scenarios within the Cooja simulator.Our systematic evaluation focuses on critical performance metrics,including Packet Delivery Ratio(PDR),Average End to End Delay(AE2ED),throughput,Expected Transmission Count(ETX),and Average Power Consumption(APC).Our findings illuminate the disruptive impact of this attack on the routing hierarchy,resulting in decreased PDR and throughput,increased AE2ED,ETX,and APC.These results underscore the urgent need for robust security measures to protect RPL-based IoT networks.Furthermore,our study emphasizes the exacerbated impact of the attack in mobile scenarios,highlighting the evolving security requirements of IoT networks. 展开更多
关键词 RPL decreased rank attacks MOBILITY random direction model
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Requirements ranking based on crowd-sourcing high-end product USs
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作者 MA Yufeng DOU Yajie +2 位作者 XU Xiangqian JIA Qingyang TAN Yuejin 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2024年第1期94-104,共11页
Based on the characteristics of high-end products,crowd-sourcing user stories can be seen as an effective means of gathering requirements,involving a large user base and generating a substantial amount of unstructured... Based on the characteristics of high-end products,crowd-sourcing user stories can be seen as an effective means of gathering requirements,involving a large user base and generating a substantial amount of unstructured feedback.The key challenge lies in transforming abstract user needs into specific ones,requiring integration and analysis.Therefore,we propose a topic mining-based approach to categorize,summarize,and rank product requirements from user stories.Specifically,after determining the number of story categories based on py LDAvis,we initially classify“I want to”phrases within user stories.Subsequently,classic topic models are applied to each category to generate their names,defining each post-classification user story category as a requirement.Furthermore,a weighted ranking function is devised to calculate the importance of each requirement.Finally,we validate the effectiveness and feasibility of the proposed method using 2966 crowd-sourced user stories related to smart home systems. 展开更多
关键词 high-end product complex system crowd-sourcing user stories topic mining requirements ranking
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Test Case Generation Evaluator for the Implementation of Test Case Generation Algorithms Based on Learning to Rank
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作者 Zhonghao Guo Xinyue Xu Xiangxian Chen 《Computer Systems Science & Engineering》 2024年第2期479-509,共31页
In software testing,the quality of test cases is crucial,but manual generation is time-consuming.Various automatic test case generation methods exist,requiring careful selection based on program features.Current evalu... In software testing,the quality of test cases is crucial,but manual generation is time-consuming.Various automatic test case generation methods exist,requiring careful selection based on program features.Current evaluation methods compare a limited set of metrics,which does not support a larger number of metrics or consider the relative importance of each metric to the final assessment.To address this,we propose an evaluation tool,the Test Case Generation Evaluator(TCGE),based on the learning to rank(L2R)algorithm.Unlike previous approaches,our method comprehensively evaluates algorithms by considering multiple metrics,resulting in a more reasoned assessment.The main principle of the TCGE is the formation of feature vectors that are of concern by the tester.Through training,the feature vectors are sorted to generate a list,with the order of the methods on the list determined according to their effectiveness on the tested assembly.We implement TCGE using three L2R algorithms:Listnet,LambdaMART,and RFLambdaMART.Evaluation employs a dataset with features of classical test case generation algorithms and three metrics—Normalized Discounted Cumulative Gain(NDCG),Mean Average Precision(MAP),and Mean Reciprocal Rank(MRR).Results demonstrate the TCGE’s superior effectiveness in evaluating test case generation algorithms compared to other methods.Among the three L2R algorithms,RFLambdaMART proves the most effective,achieving an accuracy above 96.5%,surpassing LambdaMART by 2%and Listnet by 1.5%.Consequently,the TCGE framework exhibits significant application value in the evaluation of test case generation algorithms. 展开更多
关键词 Test case generation evaluator learning to rank RFLambdaMART
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唑来膦酸对慢性阻塞性肺疾病患者骨质疏松症的临床疗效及对OPG/RANK/RANKL系统的影响
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作者 李硕 董明霞 张丽娜 《罕少疾病杂志》 2024年第9期115-117,共3页
目的 探讨唑来膦酸对慢性阻塞性肺疾病患者骨质疏松症的临床疗效及对OPG/RANK/RANKL系统的影响。方法 选取2019年5月~2022年9月在本院就诊的慢性阻塞性肺疾病合并骨质疏松症患者86例,在慢阻肺规范化治疗基础上,予以钙尔奇D,同时采用静... 目的 探讨唑来膦酸对慢性阻塞性肺疾病患者骨质疏松症的临床疗效及对OPG/RANK/RANKL系统的影响。方法 选取2019年5月~2022年9月在本院就诊的慢性阻塞性肺疾病合并骨质疏松症患者86例,在慢阻肺规范化治疗基础上,予以钙尔奇D,同时采用静脉注射的方式予以唑来膦酸注射液。统计患者治疗前后视觉模拟评分法(visual analogue scale,VAS)评分、骨密度、肺功能、骨代谢指标及相关性分析骨代谢指标关系。结果 治疗后VAS评分显著低于治疗前,腰椎BMD、股骨颈BMD均显著高于治疗前,差异均具有统计学意义(t=57.527,P<0.001;t=3.039,P=0.004;t=8.012,P<0.001)。治疗后肺功能FEV1、FEV1/FVC均优于治疗前,差异具有统计学意义(t=3.009,P=0.004;t=2.321,P=0.025)。治疗后骨代谢指标RANKL、RANKL/OPG、PINP、S-CTX、血磷均低于治疗前,差异均具有统计学意义(P<0.05),治疗前后OPG、血钙水平比较,差异无统计学意义(P>0.05)。相关性分析显示,OPG均与PINP、S-CTX呈负相关,但无显著差异(P>0.05),RANKL、RANKL/OPG均与PINP呈正相关,但无显著差异(P>0.05),但与S-CTX呈正相关,具有统计学意义(P<0.05)。结论 唑来膦酸用于治疗慢性阻塞性肺疾病合并骨质疏松症患者,可改善患者骨代谢,提高骨密度,其治疗机制与介导OPG/RANK/RANKL系统有关。 展开更多
关键词 唑来膦酸 慢性阻塞性肺疾病 骨质疏松症 临床疗效 OPG/rank/rankL系统
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Study of the OPG/RANKL/RANK signaling pathway in mice treated with sepsis-related acute kidney injury
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作者 LI Hui CHEN Wei-lin NIU Xinrong 《Journal of Hainan Medical University》 CAS 2024年第3期8-14,共7页
Objective:The objective of this study was to investigate the alterations and potential implications of the Osteoprotegerin(OPG)/Receptor Activator of Nuclear Factor-kappa B Ligand(RANKL)/Receptor Activator of Nuclear ... Objective:The objective of this study was to investigate the alterations and potential implications of the Osteoprotegerin(OPG)/Receptor Activator of Nuclear Factor-kappa B Ligand(RANKL)/Receptor Activator of Nuclear Factor-kappa B(RANK)signaling pathway factors in a murine model of sepsis-associated acute kidney injury(SA-AKI).This research aimed to offer novel insights into the mechanistic exploration of SA-AKI.Methods:The SA-AKI model group(CLP group)was established through cecal ligation and puncture surgery(CLP),while the control group consisted of sham-operated animals(Sham group)subjected only to laparotomy without cecal ligation and puncture.Blood samples were collected 24 h post-surgery,and murine kidney tissues were harvested upon euthanasia.Serum levels of Serum Creatinine(Scr)and Blood Urea Nitrogen(BUN)were quantified using assay kits.Furthermore,serum levels of interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α),and interleukin-1 beta(IL-1β)were assessed through enzyme-linked immunosorbent assay(ELISA).Renal tissue pathological alterations were examined employing hematoxylin-eosin staining(HE),and the mRNA and protein levels of OPG,RANKL,and RANK in murine kidney tissues were determined via reverse transcription-quantitative polymerase chain reaction(RT-qPCR)and Western blotting.Results:Comparative analysis revealed that,in comparison to the Sham group,the CLP group demonstrated a significant elevation in the levels of Scr,BUN,IL-6,TNF-α,and IL-1β,with statistically significant disparities(all P<0.05).Histopathological examination of the CLP group's kidneys unveiled glomerular congestion,edema,partial ischemic wrinkling,enlargement of interstitial spaces,the presence of necrotic epithelial cells in select renal tubules,tubular luminal dilation,varying degrees of interstitial edema,and infiltration by a limited number of inflammatory cells.In parallel,relative to the Sham group,the CLP group exhibited substantial upregulation in mRNA expression of OPG and RANK in renal tissues,while RANKL mRNA expression experienced marked downregulation,with statistically significant distinctions(all P<0.05).Moreover,in comparison with the Sham group,the CLP group demonstrated an elevation in protein expression of OPG and RANK in kidney tissues,whereas RANKL protein expression displayed significant downregulation,with statistically significant differences(all P<0.05).Conclusion:In a murine sepsis model,augmented expression of OPG and RANK,coupled with diminished RANKL expression,suggests the potential involvement of the OPG/RANKL/RANK signaling pathway in the pathophysiological progression of SA-AKI. 展开更多
关键词 SEPSIS Acute kidney injury OPG/rankL/rank signaling pathway
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RANKL/RANK信号通路在女性常见癌症中的研究进展
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作者 王馨冉 徐兴华 +1 位作者 辛勤 商文庆 《菏泽医学专科学校学报》 2024年第3期77-81,共5页
癌症,即恶性肿瘤,是一类严重威胁人类健康的疾病。近些年乳腺癌、宫颈癌、卵巢癌和子宫内膜癌发病率和死亡率逐年升高,严重威胁女性的生命健康[1]。为了不断优化女性癌症的诊疗方案,众多科研工作者对以上几种女性常见癌症的发病机制进... 癌症,即恶性肿瘤,是一类严重威胁人类健康的疾病。近些年乳腺癌、宫颈癌、卵巢癌和子宫内膜癌发病率和死亡率逐年升高,严重威胁女性的生命健康[1]。为了不断优化女性癌症的诊疗方案,众多科研工作者对以上几种女性常见癌症的发病机制进行了深入研究。 展开更多
关键词 rankL/rank信号通路 女性 癌症
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基于OPG/RANKL/RANK信号通路探讨傣药肾叶山蚂蝗的抗骨质疏松作用
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作者 杨丽萍 张光云 +1 位作者 陈普 段小花 《世界科学技术-中医药现代化》 CSCD 北大核心 2024年第3期769-776,共8页
目的探讨傣药肾叶山蚂蝗对去卵巢骨质疏松大鼠模型的作用及其机制。方法60只雌性SD大鼠随机分为假手术组、模型组、仙灵骨葆组(0.24 g·kg^(-1))、肾叶山蚂蝗低(1.35 g·kg^(-1))、中(2.70 g·kg^(-1))和高剂量组(5.40 g... 目的探讨傣药肾叶山蚂蝗对去卵巢骨质疏松大鼠模型的作用及其机制。方法60只雌性SD大鼠随机分为假手术组、模型组、仙灵骨葆组(0.24 g·kg^(-1))、肾叶山蚂蝗低(1.35 g·kg^(-1))、中(2.70 g·kg^(-1))和高剂量组(5.40 g·kg^(-1))。采用双侧卵巢切除术建立骨质疏松模型,药物干预14周后,检测血清中骨钙素(Bone gamma-carboxyglutamic-acid-containing Proteins,BGP)、骨保护素(Ostoeprotegerin,OPG)和碱性磷酸酶(Alkaline phosphatase,ALP)的含量,苏木精-伊红(HE)染色法观察骨组织中骨小梁的变化;实时荧光定量PCR(qPCR)检测大鼠胫骨中OPG/RANKL/RANK信号通路相关基因的表达。另取破骨细胞前体细胞株RAW264.7,分为阴性对照组、核因子κB受体活化因子配体(RANKL)组、仙灵骨葆组、肾叶山蚂蝗低、中、高剂量组。阴性对照组不加RANKL,其余各组使用50 ng·mL^(-1) RANKL诱导,药物组同时分别加入不同浓度的仙灵骨葆和肾叶山蚂蝗含药血清进行干预。10天后进行抗酒石酸酸性磷酸酶(TRAP)染色,观察破骨细胞分化情况,qPCR测定OPG/RANKL/RANK信号通路相关基因的表达。结果与假手术组比较,模型组血清中OPG含量显著降低(P<0.01),ALP和BGP显著升高(P<0.01),骨小梁显著减少,断裂,排列稀疏,骨小梁之间间距大,胫骨组织OPG mRNA表达显著减少(P<0.01),RANKL、肿瘤坏死因子受体相关蛋白6(TRAF6)、活化T-细胞核因子1(NFATc1)、组织蛋白酶K(CTK)和降钙素受体(CalcR)mRNA水平的表达升高(P<0.01),肾叶山蚂蝗干预后能显著改善以上指标。RAW264.7培养10天后,与阴性对照组比较,RANKL组破骨细胞明显增多,肾叶山蚂蝗能显著降低破骨细胞的数量,OPG/RANKL/RANK通路相关基因表达趋势和动物实验一致。结论肾叶山蚂蝗能有效改善去卵巢大鼠模型的骨质疏松,其作用机制可能是通过抑制破骨细胞增殖分化,调节OPG/RANKL/RANK信号通路来实现。 展开更多
关键词 去卵巢骨质疏松模型 肾叶山蚂蝗 OPG/rankL/rankL信号通路 RAW264.7细胞
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Low-Rank Multi-View Subspace Clustering Based on Sparse Regularization
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作者 Yan Sun Fanlong Zhang 《Journal of Computer and Communications》 2024年第4期14-30,共17页
Multi-view Subspace Clustering (MVSC) emerges as an advanced clustering method, designed to integrate diverse views to uncover a common subspace, enhancing the accuracy and robustness of clustering results. The signif... Multi-view Subspace Clustering (MVSC) emerges as an advanced clustering method, designed to integrate diverse views to uncover a common subspace, enhancing the accuracy and robustness of clustering results. The significance of low-rank prior in MVSC is emphasized, highlighting its role in capturing the global data structure across views for improved performance. However, it faces challenges with outlier sensitivity due to its reliance on the Frobenius norm for error measurement. Addressing this, our paper proposes a Low-Rank Multi-view Subspace Clustering Based on Sparse Regularization (LMVSC- Sparse) approach. Sparse regularization helps in selecting the most relevant features or views for clustering while ignoring irrelevant or noisy ones. This leads to a more efficient and effective representation of the data, improving the clustering accuracy and robustness, especially in the presence of outliers or noisy data. By incorporating sparse regularization, LMVSC-Sparse can effectively handle outlier sensitivity, which is a common challenge in traditional MVSC methods relying solely on low-rank priors. Then Alternating Direction Method of Multipliers (ADMM) algorithm is employed to solve the proposed optimization problems. Our comprehensive experiments demonstrate the efficiency and effectiveness of LMVSC-Sparse, offering a robust alternative to traditional MVSC methods. 展开更多
关键词 CLUSTERING Multi-View Subspace Clustering Low-rank Prior Sparse Regularization
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Robust Principal Component Analysis Integrating Sparse and Low-Rank Priors
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作者 Wei Zhai Fanlong Zhang 《Journal of Computer and Communications》 2024年第4期1-13,共13页
Principal Component Analysis (PCA) is a widely used technique for data analysis and dimensionality reduction, but its sensitivity to feature scale and outliers limits its applicability. Robust Principal Component Anal... Principal Component Analysis (PCA) is a widely used technique for data analysis and dimensionality reduction, but its sensitivity to feature scale and outliers limits its applicability. Robust Principal Component Analysis (RPCA) addresses these limitations by decomposing data into a low-rank matrix capturing the underlying structure and a sparse matrix identifying outliers, enhancing robustness against noise and outliers. This paper introduces a novel RPCA variant, Robust PCA Integrating Sparse and Low-rank Priors (RPCA-SL). Each prior targets a specific aspect of the data’s underlying structure and their combination allows for a more nuanced and accurate separation of the main data components from outliers and noise. Then RPCA-SL is solved by employing a proximal gradient algorithm for improved anomaly detection and data decomposition. Experimental results on simulation and real data demonstrate significant advancements. 展开更多
关键词 Robust Principal Component Analysis Sparse Matrix Low-rank Matrix Hyperspectral Image
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A Perturbation Analysis of Low-Rank Matrix Recovery by Schatten p-Minimization
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作者 Zhaoying Sun Huimin Wang Zhihui Zhu 《Journal of Applied Mathematics and Physics》 2024年第2期475-487,共13页
A number of previous papers have studied the problem of recovering low-rank matrices with noise, further combining the noisy and perturbed cases, we propose a nonconvex Schatten p-norm minimization method to deal with... A number of previous papers have studied the problem of recovering low-rank matrices with noise, further combining the noisy and perturbed cases, we propose a nonconvex Schatten p-norm minimization method to deal with the recovery of fully perturbed low-rank matrices. By utilizing the p-null space property (p-NSP) and the p-restricted isometry property (p-RIP) of the matrix, sufficient conditions to ensure that the stable and accurate reconstruction for low-rank matrix in the case of full perturbation are derived, and two upper bound recovery error estimation ns are given. These estimations are characterized by two vital aspects, one involving the best r-approximation error and the other concerning the overall noise. Specifically, this paper obtains two new error upper bounds based on the fact that p-RIP and p-NSP are able to recover accurately and stably low-rank matrix, and to some extent improve the conditions corresponding to RIP. 展开更多
关键词 Nonconvex Schatten p-Norm Low-rank Matrix Recovery p-Null Space Property the Restricted Isometry Property
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异甘草素抑制RANKL/RANK/TRAF6信号通路对骨质疏松大鼠成骨细胞分化的影响 被引量:4
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作者 张超 李强强 +3 位作者 王雄 赵辉 叶仲夺 王勇平 《中国骨质疏松杂志》 CAS CSCD 北大核心 2023年第4期488-492,508,共6页
目的探究异甘草素对骨质疏松(OP)大鼠成骨细胞分化的影响是否与调控核因子-κB受体活化体配体(RANKL)/核因子-κB受体活化体(RANK)/肿瘤坏死因子受体相关因子6(TRAF6)信号通路有关。方法采用去势法建立绝经后OP大鼠模型,造模2周后将大... 目的探究异甘草素对骨质疏松(OP)大鼠成骨细胞分化的影响是否与调控核因子-κB受体活化体配体(RANKL)/核因子-κB受体活化体(RANK)/肿瘤坏死因子受体相关因子6(TRAF6)信号通路有关。方法采用去势法建立绝经后OP大鼠模型,造模2周后将大鼠随机分为模型组、阳性组(戊酸雌二醇0.09 mg/kg)、异甘草素低(10 mg/kg)、中(20 mg/kg)、高(40 mg/kg)剂量组,每组10只,另取10只大鼠作为假手术组。给药结束后采用ELISA法检测大鼠血清中碱性磷酸酶(ALP)、雌二醇(E_(2))水平;Micro-CT扫描观察骨微结构指标;HE染色观察股骨组织病理学;免疫组化法检测大鼠股骨Runt相关转录因子2(Runx2)蛋白表达;Western Blot法检测大鼠股骨RANKL、RANK、TRAF6蛋白表达。结果与模型组比,阳性组与异甘草素各剂量组大鼠骨组织病变明显减轻,可见新生骨小梁;ALP[(107.94±9.83)U/L、(75.27±7.51)U/L、(89.35±9.14)U/L、(106.33±10.02)U/L比(53.79±5.62)U/L]、E_(2)[(27.71±2.67)pg/mL、(18.36±1.82)pg/mL、(23.65±2.28)pg/mL、(27.46±2.71)pg/mL比(14.64±1.59)pg/mL]水平,Tb.Th[(0.36±0.04)mm、(0.23±0.02)mm、(0.28±0.03)mm、(0.36±0.03)mm比(0.12±0.01)mm]、Tb.N[(4.45±0.44)1/mm、(2.67±0.27)1/mm、(3.36±0.34)1/mm、(4.41±0.44)1/mm比(1.51±0.12)1/mm]、BMD[(0.37±0.04)g/cm^(2)、(0.22±0.02)g/cm^(2)、(0.29±0.03)g/cm^(2)、(0.38±0.03)g/cm^(2)比(0.14±0.01)g/cm^(2)]、BV/TV[(11.94±1.23)%、(7.12±0.70)%、(8.49±0.85)%、(11.77±1.16)%比(5.75±0.61)%]以及Runx2表达[(0.84±0.08)、(0.41±0.04)、(0.59±0.06)、(0.82±0.08)比(0.27±0.03)]升高(P<0.05),Tb.Sp[(0.25±0.02)mm、(0.43±0.04)mm、(0.34±0.03)mm、(0.23±0.02)mm比(0.56±0.06)mm]以及RANKL[(0.42±0.04)、(0.86±0.08)、(0.64±0.06)、(0.45±0.04)比(1.09±0.11)]、RANK[(0.39±0.04)、(0.81±0.08)、(0.67±0.06)、(0.41±0.04)比(1.03±0.10)]、TRAF6[(0.47±0.05)、(0.77±0.08)、(0.61±0.06)、(0.49±0.05)比(0.96±0.09)]蛋白表达降低(P<0.05),且异甘草素呈剂量依赖性。结论异甘草素可能通过抑制RANKL/RANK/TRAF6信号通路,促进成骨细胞分化,改善股骨病变,有效发挥对OP的治疗作用。 展开更多
关键词 异甘草素 骨质疏松 成骨细胞分化 rankL/rank/TRAF6信号通路
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基于OPG/RANKL/RANK信号通路探讨益肾健骨方 防治 绝经后骨质疏松的作用机制 被引量:7
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作者 叶子丰 戎宽 +3 位作者 张信成 匡建军 万明明 许辉 《中国骨质疏松杂志》 CAS CSCD 北大核心 2023年第1期23-28,57,共7页
目的通过研究益肾健骨方对破骨细胞的影响,进一步揭示益肾健骨方对绝经后骨质疏松症的作用机制。方法大鼠去双侧卵巢造模后分为实验组(去势+益肾健骨方灌胃)、模型组(去势+生理盐水灌胃)、假手术组(假手术+生理盐水灌胃)、阳性药物对照... 目的通过研究益肾健骨方对破骨细胞的影响,进一步揭示益肾健骨方对绝经后骨质疏松症的作用机制。方法大鼠去双侧卵巢造模后分为实验组(去势+益肾健骨方灌胃)、模型组(去势+生理盐水灌胃)、假手术组(假手术+生理盐水灌胃)、阳性药物对照组(去势+阿仑膦酸钠灌胃),提取各组BMSCs进行培养、传代后备用,同时分离刚出生不久的大鼠破骨前体细胞进行体外培养,将各组骨髓间充质干细胞与破骨前体细胞共培养构建共培养体系。观察破骨细胞分化与成熟情况,并采用Real-time PCR与Western blot检测骨髓间充质干细胞与破骨前体细胞共培养体系中Wnt10b、β-Catenin、OPG、RANKL的表达情况。结果在共培养体系中模型+OC组Wnt10b、β-Catenin、OPG mRNA与蛋白表达最低,RANKL mRNA与蛋白表达最高,假手术+OC组表达最高,益肾健骨方与阳性对照物组处理后可提升Wnt10b、β-Catenin、OPG mRNA与蛋白的表达情况,且两组间比较差异无统计学意义。在共培养体系中模型+OC组RANKL mRNA与蛋白表达最高,假手术+OC组表达最低,益肾健骨方与阳性对照物组处理后可降低RANKL mRNA及蛋白的表达,且两组间比较差异无统计学意义。结论益肾健骨方可通过调节BMSCs抑制破骨细胞的形成,提高骨质量,同时通过激活OPG/RANKL/RANK信号通路抑制破骨细胞的分化与成熟,影响骨代谢从而防治PMOP。 展开更多
关键词 益肾健骨方 绝经后骨质疏松症 OPG/rankL/rank WNT/Β-CATENIN 作用机制 信号通路
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