Objective:To determine the slow-release effect of Sorafenib carried beads and its impact on the normal liver of dogs.Materials and Methods:(1)To obtain the maximal drug-carrying of beads,different sizes of beads(300-...Objective:To determine the slow-release effect of Sorafenib carried beads and its impact on the normal liver of dogs.Materials and Methods:(1)To obtain the maximal drug-carrying of beads,different sizes of beads(300-500μm and 500-700μm)were tried.Five bottles of different sizes of beads were added into 75% solution of Sorafenid-alcohol with different concentrations:Bottle a,50mg/20ml;Bottle b,100mg/20ml;Bottle c,100 mg/40ml;Bottle d,200mg/40m;Bottle e,250mg/50ml.(2)In vivo study:12 dogs were randomly divided into four groups[group A,Sorafenib carried bead(500-700μm);group B,only bead(300-500μm);group C,Lipiodol-sorafenib and four dogs in each group].Each group was treated with TAE with emulsion mentioned above.Sorafenib concentration in plasma and liver tissue was determined with HPLC respectively.Result:(1)In vitro research:Sorafenib can be dissolved into 75% alcohol and the best concentration for drug-carrying was 100mg/20ml.(2)In vivo study:①Compared with group D,the Cmax and AUC in plasma in group A and B has a significant statistics difference(p<0.05).②Sorafenib concentration in liver tissue could be determined in group A in the 3rd day and even after one week while it could not be determined in group D.Conclusion:Sorafenib can be carried in DC-Bead in a certain condition.Compared with emulsion with Sorafenib and lipiodol,DC-bead has a definite slow-release function and it is superior to lipiodol.展开更多
文摘Objective:To determine the slow-release effect of Sorafenib carried beads and its impact on the normal liver of dogs.Materials and Methods:(1)To obtain the maximal drug-carrying of beads,different sizes of beads(300-500μm and 500-700μm)were tried.Five bottles of different sizes of beads were added into 75% solution of Sorafenid-alcohol with different concentrations:Bottle a,50mg/20ml;Bottle b,100mg/20ml;Bottle c,100 mg/40ml;Bottle d,200mg/40m;Bottle e,250mg/50ml.(2)In vivo study:12 dogs were randomly divided into four groups[group A,Sorafenib carried bead(500-700μm);group B,only bead(300-500μm);group C,Lipiodol-sorafenib and four dogs in each group].Each group was treated with TAE with emulsion mentioned above.Sorafenib concentration in plasma and liver tissue was determined with HPLC respectively.Result:(1)In vitro research:Sorafenib can be dissolved into 75% alcohol and the best concentration for drug-carrying was 100mg/20ml.(2)In vivo study:①Compared with group D,the Cmax and AUC in plasma in group A and B has a significant statistics difference(p<0.05).②Sorafenib concentration in liver tissue could be determined in group A in the 3rd day and even after one week while it could not be determined in group D.Conclusion:Sorafenib can be carried in DC-Bead in a certain condition.Compared with emulsion with Sorafenib and lipiodol,DC-bead has a definite slow-release function and it is superior to lipiodol.
