Stem cell technology for antitumor drug loading and delivery in oncology

The main aim of antineoplastic treatment is to maximize patient benefit by augmenting the drug accumulation within affected organs and tissues,thus incrementing drug effects and,at the same time,reducing the damage of non-involved tissues to cytotoxic agents.Mesenchymal stromal cells(MSC)represent a group of undifferentiated multipotent cells presenting wide self-renewal features and the capacity to differentiate into an assortment of mesenchymal family cells.During the last year,they have been proposed as natural carriers for the selective release of antitumor drugs to malignant cll,s thus optimizing cytotoxic action on cancer cll,while significantly reducing adverse side efect on healthy cells.MSC chemotherapeutic drug loading and delivery is an encouraging new area of cell therapy for several tumors,especially for those with unsatisfactory prognosis and limited treatment options available.Although some experim ental models have been sucesfuly developed,phase I dinical studies are needed to confirm this potential application of cell therapy,in particular in the case of primary and secondary lung cancers.

High drug loading hydrophobic cross-linked dextran microspheres as novel drug delivery systems for the treatment of osteoarthritis

Drug delivery via intra-articular(IA)injection has proved to be effective in osteoarthritis(OA)therapy,limited by the drug efficiency and short retention time of the drug delivery systems(DDSs).Herein,a series of modified cross-linked dextran(Sephadex,S0)was fabricated by respectively grafting with linear alkyl chains,branched alkyl chains or aromatic chain,and acted as DDSs after ibuprofen(Ibu)loading for OA therapy.This DDSs expressed sustained drug release,excellent anti-inflammatory and chondroprotective effects both in IL-1βinduced chondrocytes and OA joints.Specifically,the introduction of a longer hydrophobic chain,particularly an aromatic chain,distinctly improved the hydrophobicity of S0,increased Ibu loading efficiency,and further led to significantly improving OA therapeutic effects.Therefore,hydrophobic microspheres with greatly improved drug loading ratio and prolonged degradation rates show great potential to act as DDSs for OA therapy.

Enhanced drug loading efficiency of contact lenses via salt-induced modulation

Low drug loading efficiency is one of the main obstacles hindering the application of contact lenses(CLs) as the carrier for extended ocular drug delivery. Here in this study, a simple and effective drug loading method based on salt induced modulation was proposed and demonstrated with mechanism elucidation. First of all, using poly(2-hydroxyethyl methacrylate)(p-HEMA) as the contact lens material, betaxolol hydrochloride, Diclofenac Sodium and Betaxolol Base as the model drugs with different solubility, influence of salt concentration,salt type(sodium salts of sulfate, chloride, and sulfocyanate) and drug properties in the loading solution on drug loading efficiency was investigated. Mechanism of enhanced drug loading in contact lens was further explored via studying the influence of salt on the absorption isotherm, drug solubility and water content of CLs. Applicability of this method to other CLs materials was also investigated. It was demonstrated that adjusting the ionic strength of loading solutions resulted in significant increase of drug loading in CLs. Type and concentration of the salts and solubility of the drug were the main factors influencing enhancement ratio of drug loading. The mechanism for improved drug loading was related to the reduced drug solubility in loading solutions and the reduced bound water content in contact lenses. Modulation of drug loading by adjusting ionic strength was also applicable to other CLs and the light transmittance was not affected. This method was more suitable for salt-form drugs with high solubility. In summary, adjusting ionic strength of loading solution is an economical and effective way to improve drug loading in CLs, and this simple method may also find application in other hydrogel based drug delivery systems.

Continuous melt granulation to develop high drug loaded sustained release tablet of Metformin HCl

The present work explores the application of melt granulation technology to develop a high drug loaded sustained release matrix tablet of Metformin HCl using hydroxypropylcellulose(HPC) as a hydrophilic binder and stearic acid as an extrusion aid for producing cohesive granules. This novel approach allowed the use of a minimum number of excipients to reduce the tablet size, and to enhance compressibility of the drug. This also offered a cost effective method owing to the elimination of a ‘drying step’ prevalent in wet granulation method.Moreover, this research also focuses on resolving the processability issues associated with the use of HPC Nisso-H at high drug loading. The thermal lubricants were screened for this purpose and evaluated for their impact on extrudability, granule and tablet characteristics. Stearic acid was selected as the thermal lubricant, which not only contributed to the inhibition of burst release, but also improved the flow property of the granules.The developed matrix tablet(75% drug loading) resulted in 670 mg of weight for 500 mg dose strength and showed sustained drug release over 10 h. When compared, with conventional granulation techniques, it was observed that, under identical compression force, the tablet prepared by MG exhibited superior compactibility along with tablet hardness and optimal drug release profile. FTIR suggested nonexistence of chemical interaction between the drug and the other excipients while XRD and DSC analysis revealed the crystalline state of the drug.Furthermore, the results obtained from Raman spectroscopy proved the uniform distribution of the Metformin HCl and polymer in the final dosage form. This technology leads to the manufacture of sustained release matrix formulation with reduced tablet size of a high dose,highly water soluble drug otherwise difficult to process using standard batch-granulation.

Drug Loading on Microneedles

An experimental study was carried out to investigate the amount of drugs loaded on microneedles. The microneedles were made with poly (lactic acid). Aqueous poly (vinyl alcohol) solutions were prepared as drug solutions. Two drug loading approaches, i.e., dropping and dipping, were examined. It was found that capillary number is the only relevant dimensionless group for the two methods. For the dropping approach, dried drugs will spread near the bottom of a microneedle patch provided the surface tension is low. As for the dipping approach, both a single microneedle and an array of nine microneedles were examined. For a single microneedle, high capillary rises before pulling and pulling speed are two key factors to increase the drug loading volume. For an array of microneedles, the effect of capillary rise owing to the interaction between microneedles would increase the drug loading volume several times higher than a single microneedle of the same dimension.

Development of a concentration-controlled sequential nanoprecipitation for making lipid nanoparticles with high drug loading

Lipid-based nanostructures have garnered considerable interests over the last two decades,and have achieved tremendous clinical success including thefirst clinical approval of a liposome(Doxil)for cancer therapy in 1995 and the recent COVID-19 mRNA lipid nanoparticle vaccines.Compared to liposomes which have a lipid bilayer surrounding an aqueous core,lipid nanoparticles with a particle structure have several attractive advantages for encapsulating poorly water-soluble drugs such as better stability due to the particle structure,high drug encapsulation efficiency because of a pre-or co-drug-loading strategy.While many studies have reported the synthesis of lipid nanoparticles for hydrophobic drug encapsulation,the pre-cise control of drug loading and encapsulation efficiency remains a significant challenge.This work reports a new concentration-controlled nanoprecipitation plat-form technology for fabricating lipid nanoparticles with tunable drug loading up to 70 wt%.This method is applicable for encapsulating a wide range of drugs from very hydrophobic to slightly hydrophilic.Using this facile method,nanoparticles with tunable drug loading exhibited excellent properties such as small particle size,narrow size distribution,good particle stability,showing great promise for future drug delivery applications.

Phase separation-induced nanoprecipitation for making polymer nanoparticles with high drug loading Special Collection:Distinguished Australian Researchers

Increasing drug loading remains a critical challenge in the development and translation of nanomedicine.High drug-loading nanoparticles have demonstrated unique advantages such as less carrier material used,better-controlled drug release,and improved efficacy and safety.Herein,we report a simple and efficient salt concentration screening method for making polymer nanoparticles with exceptionally high drug loading(up to 66.5 wt%)based on phase separation-induced nanoprecipitation.Upon addition of salt,phase separation occurs in a miscible solvent-water solution delaying the precipitation time of drugs and polymers to different extents,facilitating their co-precipitation thus the formation of high drug-loading nanoparticles with high encapsulation efficiency(>90%)and excellent stability(>1 month).This technology is versatile and easy to be adapted to various hydrophobic drugs,different polymers,and solvents.This salt-induced nanoprecipitation strategy offers a novel approach to fabricating polymer nanoparticles with tunable drug loading,and opens great potentials for future nanomedicines.

Preparation and in vitro Release Properties of Mercaptopurine Drug-loaded Magnetic Microspheres

Magnetic Fe304 nanoparticles were synthesized by co-precipitation method and the mercaptopurine （MER） drug-loaded magnetic microspheres were obtained through emulsion cross-linking methods. The efficiency of this approach was evaluated in terms of drug loading content （DLC）, encapsulation efficiency （EE） and delivery properties in vitro, determined by high performance liquid chromatograph （HPLC）. The microspheres showed good DLC values of 11.8%, as well as good EE values of 79.4%. The in vitro drug release study was carried out in phosphate buffer solution （PBS） simulated body fluid, at 37 ~C with pH=7.4. The release profiles showed an initial fast release rate, which decreased as time progressed and about 84 % had been released after 48 h. The experimental results indicated that the prepared magnetic microspheres may be useful for potential applications of MER for magnetically targeted chemotherapy.

Functional Amphiphilic Poly(2-oxazoline)Block Copolymers as Drug Carriers:the Relationship between Structure and Drug Loading Capacity

Poly(2-oxazoline)(POx)is a kind of polymeric amides that can be viewed as conformational isomers of polypeptides with excellent cyto-and hemo-compatibility,and is promising to be used as drug carriers.However,the drug loading capacity(DLC)of POx for many drugs is still low except several hydrophobic ones including paclitaxel(PTX).Herein,we prepared a series of amphiphilic POx block copolymers with various functional groups,and investigated the relationship between functional structures and the DLC.Functional POxs with benzyl,carboxyl,and amino groups in the side-chain were synthesized based on a poly(2-methyl-2-oxazoline)-block-poly(2-buty1-2-oxazoline-co-2-buteny1-2-oxazoline)(PMeOx-P(nBuOx-co-ButenOx),PMBEOx)precursor,followed by click reaction between vinyl and the 2-phenylethanethiol,thioglycolic acid and cysteamine.Using thin-film hydration method,eight commonly used drugs with various characteristics were encapsulated within these functional POx polymers.We found that amine-containing drugs were more easily encapsulated by POx with carboxyl groups,while amine functionalities in POx enhanced the loading capacity of drugs with carboxyl groups.In addition,n-n interactions resulted in enhanced DLC of most drugs,except several hydrophobic drugs with aromatic to total carbon ratios less than 0.5.In general,we could successfully encapsulate all the selected drugs with a DLC%over 10%using properly selected functional POxs.The above results confirm that the DLC of polymeric carriers can be adjusted by modifying the functional groups,and the prepared series of functional POxs provide an option for various drug loadings.

High drug loading polymer micelle@ZIF-8 hybrid core–shell nanoparticles through donor–receptor coordination interaction for pH/H_(2)O_(2)-responsive drug release

Smart drug delivery nanocarriers with high drug loading capacity are of great importance in the treatment of diseases,and can improve therapeutic effectiveness as well as alleviate side effects in patients.In this work,a pH and H_(2)O_(2)-responsive drug delivery platform with high doxorubicin(DOX)loading capacity has been established through coordination interaction between DOX and phenylboronic acid containing block polymer.A composited drug nanocarrier is further fabricated by growing a zeolitic imidazolate framework 8(ZIF-8)on the surface of drug-loaded polymer micelles.The study verifies that ZIF-8 shell can act as intelligent“switch”to prevent DOX leaking from core–shell nanoparticles upon H_(2)O_(2) stimulus.However,a burst drug release is detected upon pH and H_(2)O_(2) stimuli due to the further disassociation of ZIF-8 in acid solution.Moreover,the in vitro anti-cancer experiments demonstrate that the DOX-loaded core–shell nanoparticles provide effective treatment towards cancer cells but have negligible effect on normal cells,which results from the high concentration of H_(2)O_(2) and low pH in the microenvironment of tumor cells.

Amino functionalized chiral mesoporous silica nanoparticles for improved loading and release of poorly water-soluble drug

In the present paper, chiral mesoporous silica nano-cocoon(A-CMSN) functionalized with amino group was synthesized, and its loading and release of indomethacin(IMC), a poorly soluble drug, was studied. Due to the use of chiral anionic surfactants as a template, ACMSN possessed 2D hexagonal nano-cocoon morphology with curled channels on its surface, which was quite different from another 2D hexagonal mesoporous silica nanoparticles(MCM-41) with straightway channels. After being loaded into the two silica carriers by hydrogen bond, crystalline IMC converted to amorphous form, leading to the improved drug dissolution. And IMC loading capacity of A-CMSN was higher than MCM-41 because curled loading process originating from curvature chiral channels can hold more drug molecules. Compared with IMC, IMC loaded A-CMSN presented obviously fast release throughout the in vitro release experiment, while IMC loaded MCM-41 released faster than IMC at the initial 5 h then showed controlled slow release afterwards, which was closely related to the mesoporous silica nanoparticles and different channel mesostructures of these two carriers. A-CMSN possessed nano-cocoon morphology with curled 2D hexagonal channel and its channel length was shorter than MCM-41, therefore IMC molecules can easily get rid of the constraint of A-CMSN then to be surrounded by dissolution medium.

CalliSpheres可载药微球动脉栓塞化疗治疗膀胱癌并出血的疗效及安全性分析

目的 探讨CalliSpheres可载药微球动脉化疗栓塞治疗膀胱癌并出血的可行性及安全性。方法 选取接受CalliSpheres可载药微球经导管动脉化疗栓塞(DEB-TACE)治疗的11例膀胱癌并出血患者,采用改良实体瘤疗效评价标准(mRECIST)评价3个月、6个月的治疗效果,并记录患者治疗前后血尿复发情况、血液指标变化、不良反应及并发症发生情况。结果 11例患者均完成介入栓塞治疗,技术成功率100%。肉眼血尿均在术后48~72 h消失。患者首次治疗后3个月和6个月的客观溶解率(ORR)分别为72.73%和63.64%,疾病控制率DCR分别为90.91%和81.82%。随访9个月时11例患者均生存。术后并发症包括膀胱区疼痛8例,恶心、呕吐6例,发热2例,均经对症处理后缓解。未出现异位栓塞等严重并发症。结论 应用CalliSpheres载药微球动脉栓塞化疗治疗膀胱癌并出血疗效显著,安全性好,值得推广。

ZnO Incorporated Acrylamide Grafted Chitosan Based Composite Film for Advanced Wound Healing Applications

This study was carried out to prepare ZnO nanoparticles incorporated acrylamide grafted chitosan composite film for possible biomedical application especially drug loading in wound healing. ZnO nanoparticles were prepared by co-precipitation method from zinc acetate di-hydrate and incorporated in acrylamide grafted chitosan. FT-IR and TGA of the prepared composite film confirmed the successful incorporation of ZnO nanoparticles in the acrylamide-grafted polymer matrix. SEM images showed that the ZnO nanoparticles were homogeneously distributed on the porous matrix of the composite film. Water uptake and buffer uptake analysis revealed that the composite film could hold water and buffer sufficiently, which facilitated the absorption of exudate from the wound site. Amoxicillin was loaded in the prepared composite film and the maximum loading efficiency was found to be 67.33% with drug concentration of 300 ppm. In vitro studies showed greater antimicrobial activity of drug-loaded composite film compared to both pure film and standard antibiotic disc. Finally, the In vivo mouse model showed maximum healing efficiency compared to conventional gauge bandages because the loading of antibiotic in the film produced a synergistic effect and healing time was reduced.

负载EPZ6438牛血清白蛋白-壳聚糖纳米粒抑制骨肉瘤的效应

背景:骨肉瘤肿瘤干细胞激活最显著的转录因子是EZH2,据报道沉默EZH2可以抑制骨肉瘤细胞生长。研究证实,牛血清白蛋白-壳聚糖纳米粒是一种具有优异生物相容性和生物降解性的药物传递载体,且白蛋白载体可提供靶向肿瘤的药物递送功能。目的:探讨负载EPZ6438(EZH2抑制剂)血清白蛋白-壳聚糖纳米粒抑制骨肉瘤的效应及机制。方法:①制备未负载与负载EPZ6438的血清白蛋白-壳聚糖纳米粒,检测载EPZ6438血清白蛋白-壳聚糖纳米粒的药物包载率及药物释放率。②将MG-63细胞分4组培养,分别加入PBS(对照组)、血清白蛋白-壳聚糖纳米粒浸提液(空白纳米粒组)、EPZ6438溶液(游离药物组)及负载EPZ6438的血清白蛋白-壳聚糖纳米粒浸提液(载药纳米粒组),培养3 d后,利用流式细胞仪检测细胞凋亡、RT-PCR法检测细胞caspase3 mRNA的表达。③取12只裸鼠,通过腋下注射MG-63细胞悬液建立皮下荷瘤小鼠模型,造模成功后随机分4组干预,分别向肿瘤组织内注射生理盐水(对照组)、血清白蛋白-壳聚糖纳米粒溶液(空白纳米粒组)、EPZ6438溶液(游离药物组)及负载EPZ6438的血清白蛋白-壳聚糖纳米粒溶液(载药纳米粒组),每组3只。注射7 d后,观察肿瘤体积及肿瘤组织冰冻切片TUNEL染色。结果与结论:①载药纳米粒的药物包载率约为8.8%,该纳米粒在纯水中具有良好的药物释放效果,24 h药物释放量为(34.72±1.93)μg,72 h为(48.58±1.10)μg,120 h为(49.18±1.24)μg,168 h(50.25±1.13)μg,药物释放在120 h到达平台期,释放率约为97.9%;②与MG-63细胞培养3 d后,对照组、空白纳米粒组细胞凋亡率低于游离药物组、载药纳米粒组(P<0.001),caspase3 mRNA的表达低于游离药物组、载药纳米粒组(P<0.0001);③注射7 d后,载药纳米粒组裸鼠肿瘤体积小其他3组(P<0.05),肿瘤组织TUNEL染色阳性细胞比率高于其他3组(P<0.0001);④结果显示,负载EPZ6438的血清白蛋白-壳聚糖纳米粒可通过诱导肿瘤细胞的凋亡来抑制骨肉瘤生长。

脂质体的制备方法及应用的研究进展

目的综述脂质体的制备方法及应用的研究进展,旨在为脂质体的进一步研究与开发新功能提供思路。方法查阅近年来国内外关于脂质体研究的相关文献,并对其进行整理、归纳与总结。结果脂质体作为近几十年发现的一种高效载体,可由人工合成的磷脂化合物来制备,且制备方法日益完善,可根据药物的不同特性对脂质体进行结构修饰,在医药、基因工程、食品、化妆品等领域起着越来越重要的作用。结论脂质体在药物治疗及生活中有着重要的研究意义与应用前景。

单轴及同轴载盐酸米诺环素骨支架抑菌性能的评价

背景:由于单轴骨支架的药物缓释速率不稳定,近年来国内外开始寻求多结构复合型打印方式,目前有将载药缓释系统与骨移植修复技术相结合的同轴载药骨支架,其在植入体内后不仅替代了缺损骨,还可缓慢释放药物,为植入部位提供一个利于成骨的微环境。目的:探讨并评价单轴及同轴载盐酸米诺环素骨支架的体外抑菌性能。方法:采用快速成型技术分别制备单轴羟基磷灰石/丝素蛋白-聚乙烯醇支架、单轴载米诺环素的羟基磷灰石/丝素蛋白-聚乙烯醇支架、同轴羟基磷灰石/丝素蛋白-聚乙烯醇支架、同轴载米诺环素的羟基磷灰石/丝素蛋白-聚乙烯醇支架,分别命名为S1、S2、T1、T2,表征支架的形态、孔隙率、降解性能、体外缓释性能及细胞毒性。将4种骨支架浸泡于PBS中制备不同时间点(1,3,5,7,14,21,28 d)的浸提液,再将定性滤纸片置入浸提液中浸提24 h,将滤纸片分别与牙龈卟啉单胞菌、具核梭酸杆菌共培养72 h,采用琼脂扩散法检测4组支架的抑菌作用。结果与结论:①支架表征:4组支架均成型良好,扫描电镜下可见,S1、S2组微观丝材表面致密且光滑,T1、T2组微观丝材表面呈现粗糙状,孔隙率大小在40%-47%之间,均满足骨支架的要求;与S2比较,T2的缓释时间更长,且药物缓释浓度长期处于1-10μg/mL之间,更有利于抑菌及成骨;体外浸泡于PBS中10周,同轴打印骨支架的降解速率快于对应的单轴打印骨支架,并且同轴载药支架的降解率低于同轴不载药组;将4组支架浸提液分别与成骨细胞共培养,CCK-8检测显示细胞增殖率均大于75%,均满足生物相容性要求;②支架抑菌作用:S1、T1不具有抑菌性能,S2、T2具有抑菌性能,在第28天的浸提液下,S2组对牙龈卟啉单胞菌、具核梭酸杆菌的抑菌圈直径均小于T2组(P<0.05);③结果表明:同轴载米诺环素的羟基磷灰石/丝素蛋白-聚乙烯醇支架具有良好的物理性能及抑菌性能。

含松香的聚氨酯载药微球的制备及性能

采用预聚—扩链—中和法合成松香基聚氨酯(RPU),以其为载体,以5-氟尿嘧啶(5-Fu)为模型药物,通过悬浮聚合法制备了负载5-Fu的松香基聚氨酯微球(5-Fu/RPUMs)。采用单因素实验优化了药物负载条件,通过红外光谱、X射线光电子能谱、扫描电子显微镜、能谱分析等对载药微球进行表征,研究了载药微球的体外释放性能及药物释放动力学模型。结果表明:成功制备了5-Fu/RPUMs微球,平均粒径为35.0μm,表面光滑圆整且有孔。药物最佳负载条件为:ρ_(5-Fu)=120 mg/mL,致孔剂种类为正丁醇/甲苯,此条件下RPU对5-Fu的包封率达63.35%,载药量达7.60%。5-Fu/RPUMs在不同pH下表现出不同的缓释性能,具有pH敏感性;在pH=6.8的PBS缓冲溶液中,264 h后的累积释放率为65.80%,具有长效的缓释作用。

基于DLP 3D打印的高给药效率微针制备工艺

载药微针可以实现一次性快速药物递送,无需二次药物涂敷与输送过程,但其不佳的载药量与刺入率造成了给药效率差的问题。研发了一种基于数字光处理(DLP)3D打印技术的高给药效率微针(HDMN)制备工艺。通过在载药微针上构建锯齿状针体结构与阶梯状载药结构来提高载药微针的刺入率与载药量,进而提高给药效率。实验结果表明使用环氧丙烯酸酯(EA)打印微针时,当曝光时间为700 ms、打印层厚为30μm时,打印出的微针实际尺寸与理论尺寸平均误差最小为9.2%。利用旋涂法在微针表面交联聚乙二醇二丙烯酸酯(PEGDA)以改善微针生物相容性。显微镜观察发现,当交联层数为1层时,PEGDA均匀地覆盖在微针表面。石蜡膜穿刺实验结果表明,HDMN穿透了2层石蜡膜,而相同实验条件下的传统载药微针仅穿透了1层。药物释放实验结果表明,HDMN相对于传统载药微针在猪皮内留下的模拟药物浓度提升了61%。该研究为后续通过改善微针结构来优化微针性能研究提供了有益借鉴。

缓释万古霉素三维支架修复兔感染性骨软骨缺损

背景:大量研究证实组织工程支架几乎可完全修复骨软骨缺损,但当骨软骨缺损合并感染时,即使前期经过彻底的清创,单纯骨软骨组织工程支架的修复效果往往不理想。目的:制备盐酸万古霉素缓释微球丝素蛋白/壳聚糖/纳米羟基磷灰石支架,观察其对兔股骨远端感染性骨软骨缺损的修复效果。方法:①采用乳化溶剂挥发法制备盐酸万古霉素缓释微球;将不同质量(7.5,10,12.5 mg)的缓释微球分别与丝素蛋白-壳聚糖-纳米羟基磷灰石溶液混合,利用化学交联法制备盐酸万古霉素缓释微球丝素蛋白/壳聚糖/纳米羟基磷灰石支架,表征支架的孔隙率、吸水膨胀率、热水溶失率及体外药物缓释等。②将45只新西兰大白兔随机分为空白组、对照组、实验组,每组15只,均建立右后肢股骨远端骨软骨缺损并感染模型,空白组不作任何处理,对照组缺损处植入丝素蛋白-壳聚糖-纳米羟基磷灰石支架,实验组缺损处植入盐酸万古霉素缓释微球(10 mg)丝素蛋白/壳聚糖/纳米羟基磷灰石支架。术后1周,检测血液样本C-反应蛋白、白细胞水平;术后4,8,12周取术区组织,分别进行大体观察与病理学观察。结果与结论:①随着缓释微球含量的增加,支架的孔隙率降低,组间比较差异有显著性意义(P<0.05);3组支架的孔径大小、吸水膨胀率、热水溶失率比较差异均无显著性意义(P>0.05);3组支架体外均可持续释放盐酸万古霉素达30 d以上。②实验组兔血液样本C-反应蛋白、白细胞水平均低于空白组、对照组(P<0.05);实验组兔术后各时间点的大体软骨修复情况明显好于空白组、对照组;苏木精-伊红、Masson、阿利新蓝及Ⅱ型胶原免疫组化染色显示,实验组兔术后各时间点的骨软骨修复效果明显优于空白组、对照组。③结果表明,盐酸万古霉素缓释微球丝素蛋白/壳聚糖/纳米羟基磷灰石支架能有效促进开放性骨软骨缺损的修复。

养肺益气汤联合载药微球支气管动脉化疗在非小细胞肺癌治疗中的临床效果分析

目的:分析养肺益气汤联合载药微球支气管动脉化疗治疗非小细胞肺癌的临床效果。方法:选择2020年1月—2023年1月启东市中医院肿瘤科收治的82例非小细胞肺癌患者,根据随机数表法分为化疗组、联用组,各41例。其中化疗组采用载药微球支气管动脉化疗治疗,而联用组采用养肺益气汤联合载药微球支气管动脉化疗治疗。比较两组肿瘤标志物、中医症候积分、毒副作用发生率。结果:治疗前,两组肿瘤标志物比较,差异无统计学意义(P>0.05);治疗后,两组肿瘤标志物均低于治疗前,且联用组低于化疗组,差异有统计学意义(P<0.05)。治疗前,两组中医症候积分比较,差异无统计学意义(P>0.05);治疗后,两组中医症候积分均低于治疗前,且联用组低于化疗组,差异有统计学意义(P<0.05)。联用组毒副作用总发生率低于化疗组,差异有统计学意义(P<0.05)。结论:在针对非小细胞肺癌进行治疗时,在载药微球支气管动脉化疗基础上予以养肺益气汤治疗能够进一步控制癌症病变,缓解各项临床症状,并降低毒副作用发生的可能性。