目的了解2022—2023年贵州省单核细胞增生李斯特氏菌血清表型、脉冲场凝胶电泳(pulse-field gel electrophoresis,PFGE)分子分型特征和耐药情况,旨在为贵州省李斯特氏菌病的防控提供实验室数据支持并提高预警监测能力。方法收集2022年1...目的了解2022—2023年贵州省单核细胞增生李斯特氏菌血清表型、脉冲场凝胶电泳(pulse-field gel electrophoresis,PFGE)分子分型特征和耐药情况,旨在为贵州省李斯特氏菌病的防控提供实验室数据支持并提高预警监测能力。方法收集2022年1月—2023年12月贵州省致病菌识别网中心实验室监测腹泻疾病相关样本1220份,按照GB 4789.30—2016《食品安全国家标准食品卫生微生物学检验单核细胞增生李斯特氏菌检验》的执行标准分别进行分离培养,按照《国家致病菌识别网实验室监测技术手册(2022试用版)》进行实时荧光聚合酶链反应(polymerase chain reaction,PCR)检测方法鉴定,多重PCR检测血清型分型、PFGE分子分型和药敏试验。结果1220份腹泻疾病相关样本分离出424株菌株,其中单核细胞增生李斯特氏菌11株,阳性率为2.59%(11/424);11株单核细胞增生李斯特氏菌分为6种血清表型,血清型分布为1/2a、1/2b、1/2c、3a、3b和3c型;11株单核细胞增生李斯特氏菌对头孢西丁(cefoxitin,FOX)耐药率高达100%,对氯霉素(chloroamphenicol,CHL)中介率为72.72%,剩余12种药物[红霉素(erythromycin,ERY)、复方新诺明(trime-thoprim/sulfamethoxazole,T/SUL)、庆大霉素(gentamicin,GEN)、亚胺培南(imipenem,IPM)、苯唑西林(benzoxacillin,OXA)、克林霉素(clindamycin,CLI)、环丙沙星(ciprofloxacine,CIP)、达托霉素(datomycin,DAP)、氨苄西林(ampicillin,AMP)、四环素(tetracycline,TET)、万古霉素(vancomycin,VAN)、青霉素(chloramphenical,PEN)]均100%敏感;经AscⅠ酶切后11株单核细胞增生李斯特氏菌分成7种PFGE带型,相似性为40%~100%,其中3株菌相似性高达100%。结论2022—2023年贵州省发生了一起家庭聚集性李斯特氏菌病事件,遵义市肉制品中存在相同的污染来源,且污染源持续存在,贵州省具有多种致病性单核细胞增生李斯特氏菌,但尚未出现严重耐药情况。展开更多
A spatiotemporally dynamic therapy(SDT)is proposed as a powerful therapeutic modality that provides spatially dynamic responses of drug-carriers for adapting to the wound microenvironment.Herein,dynamic chitosan-poly(...A spatiotemporally dynamic therapy(SDT)is proposed as a powerful therapeutic modality that provides spatially dynamic responses of drug-carriers for adapting to the wound microenvironment.Herein,dynamic chitosan-poly(ethylene glycol)(CP)Schiff-base linkages are employed to perform SDT by directly converting a liquid drug Kangfuxin(KFX)into a gel formation.The obtained KFX-CP drug-gel with shape-adaptive property is used to treat a representative oral mucositis(OM)model in a spatiotemporally dynamic manner.The KFX-CP drug-gel creates an instructive microenvironment to regulate signaling biomolecules and endogenous cells behavior,thereby promoting OM healing by the rule of dynamically adjusting shape to fit the irregular OM regions first,and then provides space for tissue regeneration,over KFX potion control and the general hydrogel group of CP hydrogel and KFX-F127.Most interestingly,the regenerated tissue has ordered structure like healthy tissue.Therefore,the SDT provides a new approach for the design of next generation of wound dressing and tissue engineering materials.展开更多
The bioactive glass and related biomaterials have become increasingly popular, and have also attracted the research interest of many researchers in recent years due its special performance and tissue engineering appli...The bioactive glass and related biomaterials have become increasingly popular, and have also attracted the research interest of many researchers in recent years due its special performance and tissue engineering application. In this study, to create a material with a variety of properties Mg doped hollow bioactive glass (Mg-HBG) of 80SiO2-5P2O5-10CaO-5MgO system had been produced by using a sol-gel method. The porous structure nanoparticles were specifically made by employing the cetyltrimethylammonium bromide (CTAB) as a surfactant. Magnesium was selected as a doped material with HBG, because it is the most existing cations in the human body which helps for bone metabolism as well as it has antibacterial property. Based on different investigations resulted nanoparticle with the inclusion of the lower molar fractions magnesium has good tested result. For a drug model vancomycin hydrochloride (VAN) was used in this study and it has also good antibacterial activity effect. These findings help the possibility of using Mg-HBG nanoparticles to treat infectious bone abnormalities by demonstrating their compatibility with antibiotics, drug loading and release behavior.展开更多
目的对一起因食用肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件进行溯源分析。方法对8例患者进行流行病学调查,采集相关环境、食品及粪便等样本,根据国家标准进行实验室检测,经增菌后挑取可疑菌落进行生化鉴定,对检出的6株沙门...目的对一起因食用肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件进行溯源分析。方法对8例患者进行流行病学调查,采集相关环境、食品及粪便等样本,根据国家标准进行实验室检测,经增菌后挑取可疑菌落进行生化鉴定,对检出的6株沙门氏菌株采用标准诊断血清进行血清凝集试验,药敏试验及脉冲场凝胶电泳。结果该起事件发病8例,6份样本中,分离出肠炎沙门氏菌,沙门氏菌检出率食品、环境和病人粪便样本均为100%,药敏试验结果显示,耐药谱为多粘菌素E(Colistin,CT)―头孢噻肟(Cefotaxime,CTX)―四环素(Tetracycline,TET)―环丙沙星(Ciprofloxacin,CIP)―氨苄西林(Ampicillin,AMP)―氨苄西林/舒巴坦(Ampicillin/Sulbactam,AMS),均呈现6重耐药现象,对氯霉素(Chloramphenicol,CHL)、复方新诺明(Sulfamethoxazole,SXT)、厄他培南(Ertapenem,ETP)、美罗培南(Meropenem,MEM)、头孢他啶(Ceftazidime,CAZ)、头孢他啶/阿维巴坦(Ceftazidime/avibactam,CZA)、替加环素(Tigecycline,TIG)、阿奇霉素(Azithromycin,AZM)、阿米卡星(Amikacin,AMI)敏感,6株菌的脉冲场凝胶电泳(Pulse Field Gel Electrophoresis,PFGE)条带一致,同源性100%,显示为同一克隆。结论该起事件为市民食用被肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件。展开更多
目的了解2019—2021年辽宁省食品中致泻大肠埃希菌的污染状况,对分离出的致泻大肠埃希菌进行分子特征研究及耐药分析,为食源性疾病溯源和临床用药提供科学依据。方法辽宁省内采集包括水果及其制品、肉及其制品、水产品、餐饮食品等1000...目的了解2019—2021年辽宁省食品中致泻大肠埃希菌的污染状况,对分离出的致泻大肠埃希菌进行分子特征研究及耐药分析,为食源性疾病溯源和临床用药提供科学依据。方法辽宁省内采集包括水果及其制品、肉及其制品、水产品、餐饮食品等1000份样品进行致泻大肠埃希菌分离,对分离出的阳性菌采用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)进行分子特征分析,应用BioNumeries 7.6软件对菌株间的相似度聚类分析;采用微量肉汤稀释法进行药物敏感性检测。结果从1000份食品中分离出38株致泻大肠埃希菌,检出率为3.8%,其中从肉及肉制品中分离出20株,占52.6%。PFGE聚类结果显示36株肠集聚性大肠埃希菌(enteroaggre-gative Escherichia coli,EAEC)菌被分为35种带型,同源性为60%~100%。药物敏感性结果显示,38株致泻大肠埃希菌呈现30株耐药株,耐药率为78.9%,其中耐药率最高的为四环素,为71.1%(27/38);其次是氨苄西林55.3%(21/38),共发现12种多重耐药谱,多重耐药率为60.5%(23/38)。结论辽宁省致泻大肠埃希菌污染情况较为严重,且来源于肉及肉制品食品的致泻大肠埃希菌耐药情况比较严重,应引起相关部门的注意。展开更多
基金supported by Key Program of Beijing Natural Science Foundation(Z200025)National Natural Science Foundation of China(52003161)+3 种基金National Mega-project for Innovative Drugs(2019ZX09721001-007-002)Shenzhen Science and Technology Project(JCYJ20180507183842516)Fundamental Research Funds for the Central Universities(BHYC1705B,PYBZ1709)Research Projects on Biomedical Transformation of China-Japan Friendship Hospital(PYBZ1806).
文摘A spatiotemporally dynamic therapy(SDT)is proposed as a powerful therapeutic modality that provides spatially dynamic responses of drug-carriers for adapting to the wound microenvironment.Herein,dynamic chitosan-poly(ethylene glycol)(CP)Schiff-base linkages are employed to perform SDT by directly converting a liquid drug Kangfuxin(KFX)into a gel formation.The obtained KFX-CP drug-gel with shape-adaptive property is used to treat a representative oral mucositis(OM)model in a spatiotemporally dynamic manner.The KFX-CP drug-gel creates an instructive microenvironment to regulate signaling biomolecules and endogenous cells behavior,thereby promoting OM healing by the rule of dynamically adjusting shape to fit the irregular OM regions first,and then provides space for tissue regeneration,over KFX potion control and the general hydrogel group of CP hydrogel and KFX-F127.Most interestingly,the regenerated tissue has ordered structure like healthy tissue.Therefore,the SDT provides a new approach for the design of next generation of wound dressing and tissue engineering materials.
文摘The bioactive glass and related biomaterials have become increasingly popular, and have also attracted the research interest of many researchers in recent years due its special performance and tissue engineering application. In this study, to create a material with a variety of properties Mg doped hollow bioactive glass (Mg-HBG) of 80SiO2-5P2O5-10CaO-5MgO system had been produced by using a sol-gel method. The porous structure nanoparticles were specifically made by employing the cetyltrimethylammonium bromide (CTAB) as a surfactant. Magnesium was selected as a doped material with HBG, because it is the most existing cations in the human body which helps for bone metabolism as well as it has antibacterial property. Based on different investigations resulted nanoparticle with the inclusion of the lower molar fractions magnesium has good tested result. For a drug model vancomycin hydrochloride (VAN) was used in this study and it has also good antibacterial activity effect. These findings help the possibility of using Mg-HBG nanoparticles to treat infectious bone abnormalities by demonstrating their compatibility with antibiotics, drug loading and release behavior.
文摘目的对一起因食用肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件进行溯源分析。方法对8例患者进行流行病学调查,采集相关环境、食品及粪便等样本,根据国家标准进行实验室检测,经增菌后挑取可疑菌落进行生化鉴定,对检出的6株沙门氏菌株采用标准诊断血清进行血清凝集试验,药敏试验及脉冲场凝胶电泳。结果该起事件发病8例,6份样本中,分离出肠炎沙门氏菌,沙门氏菌检出率食品、环境和病人粪便样本均为100%,药敏试验结果显示,耐药谱为多粘菌素E(Colistin,CT)―头孢噻肟(Cefotaxime,CTX)―四环素(Tetracycline,TET)―环丙沙星(Ciprofloxacin,CIP)―氨苄西林(Ampicillin,AMP)―氨苄西林/舒巴坦(Ampicillin/Sulbactam,AMS),均呈现6重耐药现象,对氯霉素(Chloramphenicol,CHL)、复方新诺明(Sulfamethoxazole,SXT)、厄他培南(Ertapenem,ETP)、美罗培南(Meropenem,MEM)、头孢他啶(Ceftazidime,CAZ)、头孢他啶/阿维巴坦(Ceftazidime/avibactam,CZA)、替加环素(Tigecycline,TIG)、阿奇霉素(Azithromycin,AZM)、阿米卡星(Amikacin,AMI)敏感,6株菌的脉冲场凝胶电泳(Pulse Field Gel Electrophoresis,PFGE)条带一致,同源性100%,显示为同一克隆。结论该起事件为市民食用被肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件。
文摘目的了解2019—2021年辽宁省食品中致泻大肠埃希菌的污染状况,对分离出的致泻大肠埃希菌进行分子特征研究及耐药分析,为食源性疾病溯源和临床用药提供科学依据。方法辽宁省内采集包括水果及其制品、肉及其制品、水产品、餐饮食品等1000份样品进行致泻大肠埃希菌分离,对分离出的阳性菌采用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)进行分子特征分析,应用BioNumeries 7.6软件对菌株间的相似度聚类分析;采用微量肉汤稀释法进行药物敏感性检测。结果从1000份食品中分离出38株致泻大肠埃希菌,检出率为3.8%,其中从肉及肉制品中分离出20株,占52.6%。PFGE聚类结果显示36株肠集聚性大肠埃希菌(enteroaggre-gative Escherichia coli,EAEC)菌被分为35种带型,同源性为60%~100%。药物敏感性结果显示,38株致泻大肠埃希菌呈现30株耐药株,耐药率为78.9%,其中耐药率最高的为四环素,为71.1%(27/38);其次是氨苄西林55.3%(21/38),共发现12种多重耐药谱,多重耐药率为60.5%(23/38)。结论辽宁省致泻大肠埃希菌污染情况较为严重,且来源于肉及肉制品食品的致泻大肠埃希菌耐药情况比较严重,应引起相关部门的注意。
