Polyamines(PAs) are important endogenous plant growth regulators responding to environmental stress and mediating many physiological processes including grain filling in cereals.This study investigated whether PAs med...Polyamines(PAs) are important endogenous plant growth regulators responding to environmental stress and mediating many physiological processes including grain filling in cereals.This study investigated whether PAs mediate the effect of post-anthesis soil drying on starch granule size distribution,starch content,and weight of superior and inferior kernels of wheat(Triticum aestivum L.).Two wheat cultivars were grown in pots.Three treatments,well-watered(WW),moderate soil drying(MD) and severe soil drying(SD),were imposed from 9 days post-anthesis until maturity.PA levels in kernels and small,medium and large granules were measured.The results showed that superior kernels had much higher free spermidine(Spd) and free spermine(Spm) concentrations,larger volumes of medium starch granules,and smaller-sized large granules than did inferior kernels under all the treatments.Compared to WW,MD significantly increased the concentrations of free Spd and free Spm,activities of soluble starch synthase and granule-bound starch synthase,volume of medium granules,and starch content and kernel weight of inferior kernels,and decreased the size of large granules.SD produced the opposite effect.Application of Spd or Spm to spikes produced effects similar to those of MD,and application of an inhibitor of Spd and Spm synthesis produced effects similar to those of SD.These results suggest that PAs mediate the effect of post-anthesis soil drying on starch biosynthesis in wheat kernels by regulating key enzymes in starch synthesis and that elevated PA levels under MD increase the volume of medium granules and kernel weight of inferior kernels.展开更多
The objective of this study was to investigate whether and how exogenous abscisic acid(ABA)is involved in mediating starch accumulation in the grain and redistribution of carbohydrates during grain filling of two whea...The objective of this study was to investigate whether and how exogenous abscisic acid(ABA)is involved in mediating starch accumulation in the grain and redistribution of carbohydrates during grain filling of two wheat cultivars with different staygreen characteristics.At blooming stage,plants of Wennong 6(a staygreen cultivar)and Jimai 20(control)were sprayed with10 mg L-1abscisic acid(ABA)for 3 days.The application of ABA significantly(P<0.05)increased grain filling rate,starch accumulation rate and content,remobilization of dry matters to kernels,and 1000-grain weight of the two cultivars.Exogenous ABA markedly(P<0.05)increased grain yield at maturity,and Wennong 6 and Jiami 20 showed 14.14%and 4.86%higher compared yield than the control.Dry matter accumulation after anthesis of Wennong 6 was also significantly(P<0.05)influenced by exogenous ABA,whereas that of Jimai 20 was unchanged.Application of ABA increased endogenous zeatin riboside(ZR)content 7 days after anthesis(DAA),and spraying ABA significantly increased endogenous indole-3-acetic acid(IAA)and ABA contents from 7 to 21 DAA and decreased gibberellin(GA3)content at 14 DAA,but increased GA3content from 21 to 35 DAA.The results suggested that increased yield of staygreen was due to greater starch assimilation owing to a higher filling rate and longer grain-filling duration.展开更多
The dynamic relationship of dry matter accumulation and distribution between winter wheat root and shoot was studied under different soil water conditions. The dry matter accumulation in root was greatly influenced by...The dynamic relationship of dry matter accumulation and distribution between winter wheat root and shoot was studied under different soil water conditions. The dry matter accumulation in root was greatly influenced by water stress, so as to the final root weight of the treatment with 40% field moisture capacity(FMC) was less than 1/4 of that of the treatment with 80% FMC on average. Water stress during the 3-leaf stage to the tillering stage had the greatest influence on root, and the influence of water stress during the jointing stage to the booting stage on shoot was greater than root. However, water stress during the tillering stage to the booting stage had a balanced effect on root and shoot, and the proportion of dry matter that distributed to root and shoot was almost the same after rewatering. Water recovery during the jointing stage to booting stage could promote R/S, but the increasing degree was related to the duration of water limitation. Soil water condition had the lowest effect on R/S during the flowering stage to the filling stage and the maximal effect on R/S during the jointing stage to the heading stage, R/S of 40% FMC treatment was 20.93 and 126.09% higher than that of 60% FMC and 80% FMC treatments respectively at this period.展开更多
The photosynthetic characteristics of flag leaf and the accumulation and remobilization of pre-anthesis dry mass(DM) and nitrogen(N) in vegetable organs in nine wheat cultivars under different source-sink manipula...The photosynthetic characteristics of flag leaf and the accumulation and remobilization of pre-anthesis dry mass(DM) and nitrogen(N) in vegetable organs in nine wheat cultivars under different source-sink manipulation treatments including defoliation(DF), spike shading(SS) and half spikelets removal(SR) were investigated. Results showed that the SS treatment increased the photosynthetic rate(Pn) of flag leaf in source limited cultivar, but had no significant effect on sink limited cultivar. The SR treatment decreased the Pn of flag leaf. Grain DM accumulation was limited by source in some cultivars, in other cultivars, it was limited by sink. Grain N accumulation was mainly limited by source supply. The contribution of pre-anthesis dry mass to grain yield from high to low was stem, leaf and chaff, while the contribution of pre-anthesis N to grain N from high to low was leaf, stem and chaff. Cultivars S7221 and TA9818 can increase the contribution of remobilization of DM and N to grain at the maximum ratio under reducing source treatments, which may be the major reason for these cultivars having lower decrease in grain yield and N content under reducing source treatments.展开更多
Applying mathematic models to evaluate absorbed-N effects on dry matter production at different developmental stages would help determine proper nitrogen management according to crop demands and yield target. Two fiel...Applying mathematic models to evaluate absorbed-N effects on dry matter production at different developmental stages would help determine proper nitrogen management according to crop demands and yield target. Two field trials were carried out for establishing absorbed-N effects on dry matter production(ANEDr) model, using uniform design in 2010–2011and 2012–2013 winter wheat growing seasons in Hebei Province, China. Another field trial was carried out in 2010–2011for model validation. Dry matter and N concentration in leaf and non-leaf organs were measured at setting, jointing, anthesis, and maturity. Theory of best linear unbiased prediction(BLUP) was applied to analyse the N effects of leaf and non-leaf organs on dry matter production. Within ANEDr model, four N-affected phases at each stage were concerned,leaf absorbed-N effect before this stage, non-leaf organ absorbed-N effect before this stage,leaf absorbed-N effect at this stage, and non-leaf organ absorbed-N effect at this stage. In addition, developmental processes, genotype characters and temperature were three factors that determine each N effect. It was demonstrated that ANEDr model can precisely quantify absorbed-N effects on dry matter production with high correlation coefficient(r=0.95). Comparing with other models, ANEDr model considered both leaf and non-leaf organs according to developmental processes of winter wheat, showed higher flexibility and simplicity, thus could be applied to different environments, cultivars and crops after parameter adjustment.展开更多
The relation between technology parameters and performance index of the wheat three-stage concurrent-flow drying technology is studied by use of quadratic orthogonial rotate combinative experiment design method.The op...The relation between technology parameters and performance index of the wheat three-stage concurrent-flow drying technology is studied by use of quadratic orthogonial rotate combinative experiment design method.The optimum parameters of drying technology are obtained by nonlinear optimize corresponding regression model and test.All experiment was finished in concurrent-flow grain drying experiment table.The results of this study will be significant for designing energy-saving grain drying machine.展开更多
为提高新疆旱地春小麦产量及水分利用效率,筛选出适宜的播种方式,采用单因素随机区组试验,设置传统平作(T1)、起垄沟播(T2)、起垄覆膜沟播(T3)3个播种方式,研究不同播种方式下旱地的土壤含水量及春小麦的叶面积指数(leaf area index,LAI...为提高新疆旱地春小麦产量及水分利用效率,筛选出适宜的播种方式,采用单因素随机区组试验,设置传统平作(T1)、起垄沟播(T2)、起垄覆膜沟播(T3)3个播种方式,研究不同播种方式下旱地的土壤含水量及春小麦的叶面积指数(leaf area index,LAI)、干物质量、产量,并进一步比较土壤贮水量、耗水量及水分利用效率等。结果表明,在抽穗期之前,T2和T3处理均可显著增加旱地春小麦的LAI和干物质量;在抽穗期之后,T3处理较T2和T1处理更有利于提高旱地春小麦的LAI和干物质量。同时,T3处理可显著提高拔节期土壤0—80 cm土层的含水量和贮水量,分别较T2、T1处理显著提高32.09%、34.64%和38.20%、38.85%;T3处理降低了播种—拔节期土壤耗水量,增加了拔节期—收获期的土壤耗水量,有利于植株中、后期的生长发育。T3处理可提高旱地春小麦的有效穗数、单穗粒数、籽粒产量、水分利用效率和降水利用效率,其中产量最高为2474.43 kg·hm^(-2),较T1、T2处理分别显著增加50.13%和50.47%;水分利用效率和降水利用效率分别显著提高48.99%、51.02%和49.41%、50.15%。综上所述,起垄覆膜沟播有利于小麦增产和水分高效利用,为新疆旱地春小麦蓄水保墒和高产高效提供了理论依据及技术参考。展开更多
基金supported by grants from the National Basic Research Program of China(No.2012CB114306)the National Natural Science Foundation of China(Nos.31461143015,31271641,and 31471438)+3 种基金the National Key Technology R&D Program of China(Nos.2012BAD04B08 and 2014AA10A605)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Top Talent Supporting Program of Yangzhou University(No.2015-01)Jiangsu Creation Program for Post-graduation Students(No.KYZZ15_0364)
文摘Polyamines(PAs) are important endogenous plant growth regulators responding to environmental stress and mediating many physiological processes including grain filling in cereals.This study investigated whether PAs mediate the effect of post-anthesis soil drying on starch granule size distribution,starch content,and weight of superior and inferior kernels of wheat(Triticum aestivum L.).Two wheat cultivars were grown in pots.Three treatments,well-watered(WW),moderate soil drying(MD) and severe soil drying(SD),were imposed from 9 days post-anthesis until maturity.PA levels in kernels and small,medium and large granules were measured.The results showed that superior kernels had much higher free spermidine(Spd) and free spermine(Spm) concentrations,larger volumes of medium starch granules,and smaller-sized large granules than did inferior kernels under all the treatments.Compared to WW,MD significantly increased the concentrations of free Spd and free Spm,activities of soluble starch synthase and granule-bound starch synthase,volume of medium granules,and starch content and kernel weight of inferior kernels,and decreased the size of large granules.SD produced the opposite effect.Application of Spd or Spm to spikes produced effects similar to those of MD,and application of an inhibitor of Spd and Spm synthesis produced effects similar to those of SD.These results suggest that PAs mediate the effect of post-anthesis soil drying on starch biosynthesis in wheat kernels by regulating key enzymes in starch synthesis and that elevated PA levels under MD increase the volume of medium granules and kernel weight of inferior kernels.
基金supported by the National Natural Science Foundation of China(31271661)the National Basic Research Program of China(2009CB118602)the Public Service Sector(Agriculture)Research Program of China(201203100)
文摘The objective of this study was to investigate whether and how exogenous abscisic acid(ABA)is involved in mediating starch accumulation in the grain and redistribution of carbohydrates during grain filling of two wheat cultivars with different staygreen characteristics.At blooming stage,plants of Wennong 6(a staygreen cultivar)and Jimai 20(control)were sprayed with10 mg L-1abscisic acid(ABA)for 3 days.The application of ABA significantly(P<0.05)increased grain filling rate,starch accumulation rate and content,remobilization of dry matters to kernels,and 1000-grain weight of the two cultivars.Exogenous ABA markedly(P<0.05)increased grain yield at maturity,and Wennong 6 and Jiami 20 showed 14.14%and 4.86%higher compared yield than the control.Dry matter accumulation after anthesis of Wennong 6 was also significantly(P<0.05)influenced by exogenous ABA,whereas that of Jimai 20 was unchanged.Application of ABA increased endogenous zeatin riboside(ZR)content 7 days after anthesis(DAA),and spraying ABA significantly increased endogenous indole-3-acetic acid(IAA)and ABA contents from 7 to 21 DAA and decreased gibberellin(GA3)content at 14 DAA,but increased GA3content from 21 to 35 DAA.The results suggested that increased yield of staygreen was due to greater starch assimilation owing to a higher filling rate and longer grain-filling duration.
基金supported by the National Natural Science Foundation of China(49971042).
文摘The dynamic relationship of dry matter accumulation and distribution between winter wheat root and shoot was studied under different soil water conditions. The dry matter accumulation in root was greatly influenced by water stress, so as to the final root weight of the treatment with 40% field moisture capacity(FMC) was less than 1/4 of that of the treatment with 80% FMC on average. Water stress during the 3-leaf stage to the tillering stage had the greatest influence on root, and the influence of water stress during the jointing stage to the booting stage on shoot was greater than root. However, water stress during the tillering stage to the booting stage had a balanced effect on root and shoot, and the proportion of dry matter that distributed to root and shoot was almost the same after rewatering. Water recovery during the jointing stage to booting stage could promote R/S, but the increasing degree was related to the duration of water limitation. Soil water condition had the lowest effect on R/S during the flowering stage to the filling stage and the maximal effect on R/S during the jointing stage to the heading stage, R/S of 40% FMC treatment was 20.93 and 126.09% higher than that of 60% FMC and 80% FMC treatments respectively at this period.
基金supported by the Special Fund for Agroscientific Research in the Public Interest in China (201303133, 201203031)the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD16B14)+1 种基金the Construction of Modern Agricultural Industrial Technology System, Ministry of Agriculture, Chinathe Beijing Higher Education Young Elite Teacher Project, China (YETP0300)
文摘The photosynthetic characteristics of flag leaf and the accumulation and remobilization of pre-anthesis dry mass(DM) and nitrogen(N) in vegetable organs in nine wheat cultivars under different source-sink manipulation treatments including defoliation(DF), spike shading(SS) and half spikelets removal(SR) were investigated. Results showed that the SS treatment increased the photosynthetic rate(Pn) of flag leaf in source limited cultivar, but had no significant effect on sink limited cultivar. The SR treatment decreased the Pn of flag leaf. Grain DM accumulation was limited by source in some cultivars, in other cultivars, it was limited by sink. Grain N accumulation was mainly limited by source supply. The contribution of pre-anthesis dry mass to grain yield from high to low was stem, leaf and chaff, while the contribution of pre-anthesis N to grain N from high to low was leaf, stem and chaff. Cultivars S7221 and TA9818 can increase the contribution of remobilization of DM and N to grain at the maximum ratio under reducing source treatments, which may be the major reason for these cultivars having lower decrease in grain yield and N content under reducing source treatments.
基金supported by the Special Fund for Agro-Scientific Research in the Public Interest, China (201203031)the China Agriculture Research System (CARS-02-26)
文摘Applying mathematic models to evaluate absorbed-N effects on dry matter production at different developmental stages would help determine proper nitrogen management according to crop demands and yield target. Two field trials were carried out for establishing absorbed-N effects on dry matter production(ANEDr) model, using uniform design in 2010–2011and 2012–2013 winter wheat growing seasons in Hebei Province, China. Another field trial was carried out in 2010–2011for model validation. Dry matter and N concentration in leaf and non-leaf organs were measured at setting, jointing, anthesis, and maturity. Theory of best linear unbiased prediction(BLUP) was applied to analyse the N effects of leaf and non-leaf organs on dry matter production. Within ANEDr model, four N-affected phases at each stage were concerned,leaf absorbed-N effect before this stage, non-leaf organ absorbed-N effect before this stage,leaf absorbed-N effect at this stage, and non-leaf organ absorbed-N effect at this stage. In addition, developmental processes, genotype characters and temperature were three factors that determine each N effect. It was demonstrated that ANEDr model can precisely quantify absorbed-N effects on dry matter production with high correlation coefficient(r=0.95). Comparing with other models, ANEDr model considered both leaf and non-leaf organs according to developmental processes of winter wheat, showed higher flexibility and simplicity, thus could be applied to different environments, cultivars and crops after parameter adjustment.
文摘The relation between technology parameters and performance index of the wheat three-stage concurrent-flow drying technology is studied by use of quadratic orthogonial rotate combinative experiment design method.The optimum parameters of drying technology are obtained by nonlinear optimize corresponding regression model and test.All experiment was finished in concurrent-flow grain drying experiment table.The results of this study will be significant for designing energy-saving grain drying machine.
文摘为提高新疆旱地春小麦产量及水分利用效率,筛选出适宜的播种方式,采用单因素随机区组试验,设置传统平作(T1)、起垄沟播(T2)、起垄覆膜沟播(T3)3个播种方式,研究不同播种方式下旱地的土壤含水量及春小麦的叶面积指数(leaf area index,LAI)、干物质量、产量,并进一步比较土壤贮水量、耗水量及水分利用效率等。结果表明,在抽穗期之前,T2和T3处理均可显著增加旱地春小麦的LAI和干物质量;在抽穗期之后,T3处理较T2和T1处理更有利于提高旱地春小麦的LAI和干物质量。同时,T3处理可显著提高拔节期土壤0—80 cm土层的含水量和贮水量,分别较T2、T1处理显著提高32.09%、34.64%和38.20%、38.85%;T3处理降低了播种—拔节期土壤耗水量,增加了拔节期—收获期的土壤耗水量,有利于植株中、后期的生长发育。T3处理可提高旱地春小麦的有效穗数、单穗粒数、籽粒产量、水分利用效率和降水利用效率,其中产量最高为2474.43 kg·hm^(-2),较T1、T2处理分别显著增加50.13%和50.47%;水分利用效率和降水利用效率分别显著提高48.99%、51.02%和49.41%、50.15%。综上所述,起垄覆膜沟播有利于小麦增产和水分高效利用,为新疆旱地春小麦蓄水保墒和高产高效提供了理论依据及技术参考。