Separation and Purification of Total Phloroglucinols in Dryopteris crassirhizoma with DM-130 Macroporous Adsorption Resin

To improve the purity of the total phloroglucinols from Dryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols from Dryopteris crassirhizoma were studied with DM-130 macroporous adsorption resin in this study. Adsorption rate, elution rate and purity of the total phloroglucinols were used as indexes to investigate the adsorption and desorption capacity of the total phloroglucinols with DM-130 macroporous adsorption resin. Through the study, the optimum sample concentration of the total phloroglucinols and maximum sample volume were 1.5 mg·mL^-1 and 7 BV （210 mL）, respectively. The optimum desorption conditions were achieved by using 80% ethanol as desorption solvent at elution flow rate of 1.0 mL·min^-1. The result showed DM-130 macroporous adsorption resin performed effective adsorption and desorption. After purification, the purity of the total phloroglucinols increased by 11.5-fold.

绵马贯众水和乙醇提取物抗病毒的实验研究

目的观察绵马贯众水、乙醇两种提取物的抗病毒作用。方法 (1)离体实验:采用致细胞病变法观察绵马贯众水、乙醇两种提取物对流感病毒(FM1株)、呼吸道合胞病毒(Long)、副流感病毒(Ⅰ型、Ⅲ型)、腺病毒(AD3)的抑制作用,计算药物半数有效浓度(EC50)、治疗指数(TI);(2)在体实验:绵马贯众提取物按25%、12.5%、6.25%原液的浓度设高、中、低3个剂量给药,并设立正常对照组和病毒对照组,观察绵马贯众水、乙醇两种提取物对滴鼻感染FM1株流感病毒的昆明种小鼠肺指数的影响。结果绵马贯众水、乙醇两种提取物对流感病毒(FM1株)、呼吸道合胞病毒(Long)、副流感病毒(Ⅰ型、Ⅲ型)、腺病毒(AD3)均具有一定的抗病毒作用,绵马贯众水提取物对上述病毒的EC50分别为110.58、141.72、126.21、152.66、182.55 ng/L;TI分别为34.94,14.21,30.62,25.31,21.17。绵马贯众乙醇提取物对上述病毒的EC50分别为152.08、168.27、132.28、135.26、156.38 ng/L;TI分别为14.24,11.12,16.37,16.01,13.84。绵马贯众水提物高、中剂量组、乙醇提取物高剂量组降低感染FM1株的小鼠肺指数,与病毒对照组比较差异有统计学意义(P<0.05,0.01)。结论绵马贯众水、乙醇两种提取物均具有一定的抗病毒作用,可减低病毒引起的肺损伤。

绵马贯众中绵马贯众素大孔树脂纯化工艺研究

目的:考察大孔吸附树脂对绵马贯众中绵马贯众素的吸附分离性能。方法:采用HPLC测定绵马贯众素含量,静态吸附分离法确定适合的大孔吸附树脂,动态吸附分离法确定分离条件。结果:DM130大孔吸附树脂对绵马贯众素有良好的吸附分离性能,其分离工艺条件为:绵马贯众素上样质量浓度为0.683 mg·ml^(-1),最大上样量为6.147 mg·ml^(-1)树脂,5BV水及30%乙醇先后洗去杂质,5BV95%乙醇洗脱。结论:DM130树脂交换吸附绵马贯众中绵马贯众素的纯化方法可取,具有良好的应用前景。

阴离子交换树脂分离纯化绵马贯众总多酚的工艺研究

目的研究阴离子交换树脂分离纯化绵马贯众总多酚的工艺条件。方法分别以绵马贯众素ABBA、绵马贯众总多酚的比吸附量、洗脱率、质量分数为指标,优选绵马贯众总多酚的最佳纯化工艺。结果最佳工艺为绵马贯众药材提取液以6 BV/h的体积流量通过201×7氢氧型阴离子交换树脂柱(柱径与柱高比为1∶7),树脂饱和后,水洗至中性,再用3%氯化钠的60%乙醇溶液以6 BV/h的体积流量洗脱,洗脱9倍量树脂柱体积。绵马贯众素ABBA及绵马贯众总多酚洗脱率分别为90.1%和91.1%,绵马贯众素ABBA、绵马贯众总多酚的质量分数分别为29.4%、49.2%。结论采用201×7氢氧型阴离子交换树脂纯化绵马贯众总多酚效率高,成本低,具有广阔的应用前景。