D sR ed-M it是一种特异性定位于线粒体的荧光分子探针。本文重点探讨了D sR ed-M it探针在观察细胞凋亡形态学、以及凋亡过程中动态分子调控过程研究中的应用。实验结果表明,在细胞凋亡过程中应用该探针标记线粒体,能够直观地监测线粒...D sR ed-M it是一种特异性定位于线粒体的荧光分子探针。本文重点探讨了D sR ed-M it探针在观察细胞凋亡形态学、以及凋亡过程中动态分子调控过程研究中的应用。实验结果表明,在细胞凋亡过程中应用该探针标记线粒体,能够直观地监测线粒体肿胀的形态变化,以及细胞凋亡的重要蛋白--Bax蛋白转位线粒体和细胞色素C释放的动态过程。展开更多
Fusarium verticillioides was labeled with DsRed via Agrobacterium tumefaciens-mediated transformation to examine differences in colonization and reactions of resistant and susceptible inbred lines of maize(Zea mays L....Fusarium verticillioides was labeled with DsRed via Agrobacterium tumefaciens-mediated transformation to examine differences in colonization and reactions of resistant and susceptible inbred lines of maize(Zea mays L.). The extent of systemic colonization of F. verticillioides in roots from maize lines either resistant or susceptible to the fungus was studied by visualizing the red fluorescence produced by the fungus expressing DsRed. The difference in quantities of colony forming units(CFU) in roots and basal stems, production of fumonisin B1, and pH of root were determined. Although F. verticillioides colonized both resistant and susceptible lines, differences were observed in the pattern and extent of fungal colonization in the two types of maize lines. The fungus colonized the susceptible lines producing mosaic patterns by filling the individual root cells with hyphae. Such a pattern of colonization was rarely observed in resistant lines, which were less colonized by the fungus than the susceptible lines in terms of CFUs. The production of mycotoxin fumonisin B1 in roots from different lines was closely correlated with the amount of F. verticillioides colonization, rather than the pH or amylopectin concentrations in the root. The findings from this study contribute to a better understanding of the defense mechanism in resistant maize lines to F. verticillioides.展开更多
文摘D sR ed-M it是一种特异性定位于线粒体的荧光分子探针。本文重点探讨了D sR ed-M it探针在观察细胞凋亡形态学、以及凋亡过程中动态分子调控过程研究中的应用。实验结果表明,在细胞凋亡过程中应用该探针标记线粒体,能够直观地监测线粒体肿胀的形态变化,以及细胞凋亡的重要蛋白--Bax蛋白转位线粒体和细胞色素C释放的动态过程。
基金the National Natural Science Foundation (31170080)China Agricultural Research Service (CARS-02) was greatly appreciated
文摘Fusarium verticillioides was labeled with DsRed via Agrobacterium tumefaciens-mediated transformation to examine differences in colonization and reactions of resistant and susceptible inbred lines of maize(Zea mays L.). The extent of systemic colonization of F. verticillioides in roots from maize lines either resistant or susceptible to the fungus was studied by visualizing the red fluorescence produced by the fungus expressing DsRed. The difference in quantities of colony forming units(CFU) in roots and basal stems, production of fumonisin B1, and pH of root were determined. Although F. verticillioides colonized both resistant and susceptible lines, differences were observed in the pattern and extent of fungal colonization in the two types of maize lines. The fungus colonized the susceptible lines producing mosaic patterns by filling the individual root cells with hyphae. Such a pattern of colonization was rarely observed in resistant lines, which were less colonized by the fungus than the susceptible lines in terms of CFUs. The production of mycotoxin fumonisin B1 in roots from different lines was closely correlated with the amount of F. verticillioides colonization, rather than the pH or amylopectin concentrations in the root. The findings from this study contribute to a better understanding of the defense mechanism in resistant maize lines to F. verticillioides.
