含DsRed类似生色团的红色荧光蛋白的发展及应用

自从绿色荧光蛋白(GFP)被发现以来,荧光蛋白在生物医学领域已经成为一种重要的荧光成像工具.随着红色荧光蛋白DsRed的出现,各种优化的DsRed突变体和远红荧光蛋白也不断涌现.其中荧光蛋白生色团的形成机制对改建更优的荧光蛋白变种影响很大,对于红色荧光蛋白而言,大多数的红色荧光蛋白的生色团类型为DsRed类似生色团,在此基础上又出现了Far-redDsRed类似生色团.目前,含DsRed类似生色团的荧光蛋白主要有单体红色荧光蛋白、光转换荧光蛋白、斯托克斯红移蛋白、荧光计时器等.这些优化的荧光蛋白作为分子探针可以实现对活细胞、细胞器或胞内分子的时空标记和追踪,已经在生物工程学、细胞生物学、基础医学领域得到广泛应用.本文综述了含DsRed类似生色团的荧光蛋白的研究进展及其应用,以及由此发展起来的远红荧光蛋白在活体显微成像技术中的应用,并展望了荧光探针技术研究的新方向.

烯二炔抗肿瘤抗菌素的合成进展

当前,自然科学正在经历着一场重大的转变。化学发展的主要趋势之一就是利用化学的多重性,去探索生命科学中的复杂问题。其中,复杂并具有生物活性天然物质的合成无疑扮演着一个十分重要的角色。

Tuning the solid-state emission of the analogous GFP chromophore by varying alkyl chains in the imidazolinone ring

New analogues of green fluorescent protein (GFP) chromophore mGFP-Cn (n = 1, 3, 5, 11) with alkyl chains of different lengths in the imidazolinone rings were synthesized and their crystal structures were determined. These GFP-like chromophores are all emissive in the solid state. And the solid-state emission quantum yields of increase by extending the lengths of alkyl chains, owing to the fact that the intermolecular pi-pi interactions are significantly weakened based on their crystal structures.

Tuning the solid-state emission of the analogous GFP chromophore by varying alkyl chains in the imidazolinone ring

New analogues of green fluorescent protein(GFP)chromophore mGFP-Cn(n=1,3,5,11)with alkyl chains of different lengths in the imidazolinone rings were synthesized and their crystal structures were determined.These GFP-like chromophores are all emissive in the solid state.And the solid-state emission quantum yields of increase by extending the lengths of alkyl chains,owing to the fact that the intermolecular pi-pi interactions are significantly weakened based on their crystal structures.