Different Foliar Fertilizer Treatments Affect the Yield and Quality of Brassica napus L.for Both Vegetable and Oil Dual-Use Rape

Field experiments were carried out to study the effect of different foliar fertilizer treatments on the agronomic traits,yield,and quality of Brassica napus L.for both vegetable and oil dual-use rape.The seedlings of‘Youtai 929’,‘Jinyoutai 1’,and‘Zhongshuang 11’were sprayed with potassium dihydrogen phosphate,naphthylacetic acid,and selenium fertilizer,and the agronomic traits,yield,and quality were determined.The results showed that the naphthylacetic acid treatment had the highest indexes before winter,followed by the selenium fertilizer treatment.At the harvesting stage,the plant height,number of siliquae,yield per plant,and number of effective branches on the main stem of the three varieties were the highest in the naphthylacetic acid treatment,followed by those in the potassium dihydrogen phosphate treatment.The fresh shoot quality was the best in the selenium fertilizer treatment and the second in the naphthylacetic acid treatment.‘Jinyoutai 1’applied with naphthylacetic acid had the highest economic benefit of 4971.78 CNY/667m2,which was 642.32 CNY/667m2 higher than that of the control.The findings suggested that naphthylacetic acid was suitable to be used as the foliar fertilizer for the seedlings of B.napus varieties for both vegetable and oilseed,and the additional application of selenium fertilizer demonstrated better effect.

How to promote cooperative innovation in environmentally friendly technology:a case of agricultural biotechnology in China

This study explored the factors influencing cooperative innovation in environmentally friendly agricultural biotechnology in China.First,we constructed an evolutionary game model comprising the factors of net income of cooperative innovation,net income of independent innovation,market constraints,and government subsidies.Using MATLAB simulation,we assigned different values to the aforementioned variables to explore the evolutionary trend of innovators’willingness to cooperate.Results showed that when the values of net income of cooperative innovation,net income of independent innovation,market constraints,and government subsidies exceeded the threshold,innovators’willingness to cooperate was significantly enhanced.Furthermore,the proportion of innovators who cooperated with others gradually increased to 100%;otherwise,it gradually decreased to 0%.Comparing the simulation curve with the real evolution curve of cooperative innovation in agricultural biotechnology in China,we found that the gradual decline in the willingness to cooperate could be due to insufficient subsidies for cooperative innovation,low income from cooperative innovation,weak profitability of innovators,and weak market constraints.

Tapping Wet Potential China and Malaysia join hands to develop marine biotechnology

Kappaphycus alvarezii(K.alvarezii)is a perennial large algae native to tropical and subtropical regions.With a slender thallus,it branches better at the upper part than the lower end.The sea moss is mainly found in Hainan Province,China,and Southeast Asian countries such as Malaysia and the Philippines because it grows best in the subtidal zone of clean.

Reform of Teaching Practice of Modern Instrumental Analysis in Biotechnology Programs

Through the online and offline practical reform of the Modern Instrumental Analysis course of biotechnology majors from teaching content,teaching method,teaching demonstration to teaching effect,the traditional single offline lecture is transformed into diversified and interactive modern teaching.The practical reform enriches and optimizes the course content,perfects and improves the course assessment system,and improves the teaching quality.It achieves the student-centered and application-oriented teaching goal,and also provides reference for further cultivating high-quality applied talents.

Biotechnology ofα-linolenic acid in oilseed rape(Brassica napus)using FAD2 and FAD3 from chia(Salvia hispanica)

α-Linolenic acid(ALA,18:3Δ9,12,15)is an essential fatty acid for humans since it is the precursor for the biosynthesis of omega-3 long-chain polyunsaturated fatty acids(LC-PUFA).Modern people generally suffer from deficiency of ALA because most staple food oils are low or lack ALA content.Biotechnological enrichment of ALA in staple oil crops is a promising strategy.Chia(Salvia hispanica)has the highest ALA content in its seed oil among known oil crops.In this study,the FAD2 and FAD3 genes from chia were engineered into a staple oil crop,oilseed rape(Brassica napus),via Agrobaterium tumefaciens-mediated transformation of their LP4-2A fusion gene construct driven by the seed-specific promoter P_(NapA).In seeds of T0,T1,and T2 lines,the average ALA contents were 20.86,23.54,and 24.92%,respectively,which were 2.21,2.68,and 3.03 folds of the non-transformed controls(9.42,8.78,and 8.22%),respectively.The highest seed ALA levels of T0,T1,and T2 plants were 38.41,35.98,and 39.19%respectively,which were 4.10-4.77 folds of the respective controls.FA-pathway enzyme genes(BnACCD,BnFATA,BnSAD,BnSCD,BnDGAT1,BnDGAT2,and BnDGAT3)and positive regulatory genes(BnWRI1,BnLEC1,BnL1L,BnLEC2,BnABI3,BnbZIP67,and BnMYB96)were all significantly up-regulated.In contrast,BnTT1,BnTT2,BnTT8,BnTT16,BnTTG1,and BnTTG2,encoding negative oil accumulation regulators but positive secondary metabolism regulators,were all significantly down-regulated.This means the foreign ShFAD2-ShFAD3 fusion gene,directly and indirectly,remodeled both positive and negative loci of the whole FA-related network in transgenic B.napus seeds.

Production of Biotechnology Fulvic Acid from Fermented Corn Straw by Microbial Community LCM9 and Its Application Effect

[Objective] The aim was to evaluate the feasibility of biotechnology fulvic acid produced by microbial community LCM9 by the use of corn straws as substrate and its application effect.[Method]Microbial community was obtained from the nature samples,and corn straws were used as fermentation substrate.The yield of biotechnology fulvic acid and its application effect was evaluated.[Result]The yield of biotechnology fulvic acid was up to 15.7% when the water content was 75.0% and fermented after 7 d using corn straws as the only source of nutrition.After 500 mg/kg of biotechnology fulvic acid was added for 5 d,the root length and bud length could be increased by 59.1% and 97.1%,respectively.[Conclusion]A higher yield of biotechnology fulvic acid could be obtained by the method in this experiment and the product could enhance the growth of wheat seedlings.

“Essentials of Biotechnology”来华留学英语授课品牌课程建设纪实

经过十多年的建设,"Essentials of Biotechnology"已建设成为江南大学乃至江苏省来华留学生英语授课品牌课程。该课程针对来华留学生学科背景有较大差异,英语能力参差不齐和课程知识涉及面广,知识点较多,理论性强等问题,在原有双语课程教学的基础上,通过优化教材和教学内容,打造双向教学模式和建设多元评价体系并持续改进,构建了多层次启发式、互动、研讨相结合的复合教学体系,显著增强留学生专业基础知识水平和自主学习能力,为后续课程学习和研究工作的开展奠定了良好的基础。教学实践结果表明,该教学体系对提高该类课程的学习成效和课程质量,具有较为显著的示范效应。

生物技术(Biotechnology)在海水养殖业中的应用

作为人类开发和利用海洋生物资源的一种重要形式,海水养殖业正逐步成为我国海洋水产业的支柱产业,但目前面临着诸多问题的挑战。本文从持续发展的角度出发,针对严重影响我国海水养殖业稳定和持续发展的品种、病害和生态环境问题,探讨了应用海洋生物技术来解决这些“瓶颈”问题的作用及发展前景。

Chinese public understanding of the use of agricultural biotechnology—A case study from Zhejiang Province of China

This study explores the Chinese public’s perceptions of, and attitudes to, agriculture and food applications of bio-technology; and investigates the effect of socio-demographic factors on attitudes. A questionnaire survey and interviews were used in an attempt to combine quantitative analysis with qualitative review. The main finding of this study is that the Chinese population has a superficial, optimistic attitude to agricultural biotechnology; and that, in accordance with public attitudes, a cautious policy, with obligatory labelling, should be adopted. The study reveals that education is the factor among socio-demographic variables with the strongest impact on public attitudes. Higher education leads to a more positive evaluation of GM (genetically modified) foods and applications of biotechnology with respect to usefulness, moral acceptability, and suitability for encouragement. In addition, public attitudinal differences depend significantly on area of residence. Compared with their more urban compatriots, members of the public in less developed areas of China have more optimistic attitudes, perceive more benefits, and are more risk tolerant in relation to GM foods and agricultural biotechnology. Finally we obtained a very high rate of “don’t know” answers to our survey questions. This suggests that many people do not have settled attitudes, and correspondingly, that the overall public attitude to agricultural biotechnology and GM foods in China is at present somewhat unstable.

Application of Biotechnology in Environmental Remediation of Mining Area

Based on the characteristics of the ecological environmental remediation of mining area, the application of biotechnology was proposed to do the remediation of the polluted environment in the mining area, mainly including the treatment of gangue dump and tailing heap, improvement of reclamation soil as well as the re- mediation of contaminated soil. Finally, the application and deveiopment trend of biotechnology in environmental remediation and soil remediation of mining area were pointed out.

Geminiviruses and their application in biotechnology

Being a major class of single-stranded DNA viruses, geminiviruses are mostly studied due to their catastrophic infectious effect on crops. These DNA viruses are characteristic for their ability in quickly multiplying viral genetic materials without integrating into the genome of plants, which makes them ideal for developing viral vectors for plants bioengineering. Geminivirus-derived vectors can be classified into expression vectors and virus-induced gene silencing（VIGS） vectors. Details of the design, construction, application and improvements of these geminivirus vectors are summarized and discussed.

Success Factors of Biotechnology Industry Based on Triangular Fuzzy Number

Based on the theory of competitive advantage and value chain, this paper establishes the indicator system, and develop the strategic framework using the fuzzy Delphi method. Then the triangular fuzzy number model is established using Fuzzy Analytic Hierarchy Process, and the key factors influencing biotechnology industry are extracted. The results show that in terms of weight, the key factors influencing the success of biotechnology industry are sequenced as follows: "open innovation capacity", "quality and cost control ability", "advanced customer-oriented product manufacturing capacity", "technology R & D personnel's capacity", "brand image building capacity", "logistics and sales capacity", "grasping the market demand trends". The manufacturers and government decision-making body can use this as the basis, to promote the development of the biotechnology industry.

Construction and Exploration of Teaching Reform about the Horticultural Plant Biotechnology Course

Horticultural Plant Biotechnology is a core course of undergraduate teaching of horticulture with high practicality.For students,its contents are too complex and abstract to master.In order to strengthen the teaching effect of the course,stimulate students’interest in learning,improve students’ability to analyze and solve problems,this article makes an in-depth analysis of the complicated and abstract knowledge points,the endless emergence of new knowledge,the disconnection between practice and experimental teaching in the Horticultural Plant Biotechnology course.Based on the analysis,it puts forward the reasonable and blend methods of online teaching,to cultivate innovative and practical ability of students.

Raman spectroscopy as process analytical tool in downstream processing of biotechnology

Downstream processing or product recovery plays a vital role in the development of bioprocesses.To improve the bioprocess efficiency,some unconventional methods are much required.The continuous manufacturing in downstream processing makes the Process Analytical Technologies(PATs)as an important tool.Monitoring and controlling bioprocess are an essential factor for the principles of PAT and quality by design.Spectroscopic methods can apply to monitor multiple analytes in real-time with less sample processing with significant advancements.Raman spectroscopy is an extensively used technique as an analytical and research tool owing to its modest process form,non-destructive,non-invasive optical molecular spectroscopic imaging with computer-based analysis.Generally,its application is essential for the analysis and characterization of biological samples,and it is easy to operate with minimal sample.The innovation on various types of enhanced Raman spectroscopy was designed to enhance the Raman analytical technique.Raman spectroscopy could couple with chemometrics to provide reliable alternative analysis method of downstream process analysis.Thus,this review aims to provide useful insight on the application of Raman spectroscopy for PAT in downstream processing of biotechnology and Raman data analysis in biological fields.

Advanced Biotechnology for Cell Cryopreservation

Cell cryopreservation has evolved as an important technology required for supporting various cell-based applications,such as stem cell therapy,tissue engineering,and assisted reproduction.Recent times have witnessed an increase in the clinical demand of these applications,requiring urgent improvements in cell cryopreservation.However,cryopreservation technology suff ers from the issues of low cryopreservation effi ciency and cryoprotectant(CPA)toxicity.Application of advanced biotechnology tools can signifi cantly improve post-thaw cell survival and reduce or even eliminate the use of organic solvent CPAs,thus promoting the development of cryopreservation.Herein,based on the diff erent cryopreservation mechanisms available,we provide an overview of the applications and achievements of various biotechnology tools used in cell cryopreservation,including trehalose delivery,hydrogel-based cell encapsulation technique,droplet-based cell printing,and nanowarming,and also discuss the associated challenges and perspectives for future development.

Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology

Immobilized molecules using biomaterials and nanobiotechnology is a very interesting topic that touching almost all aspects of our life. It uses the sciences of biology, chemistry, physics, materials engineering and computer science to develop instruments and products that are at the cutting edge of some of today’s most promising scientific frontiers. In this review article, the author based on his experience in this arena has tried to focus on some of the supports for im-mobilization;the most important molecules to be immobilized such as DNA, cells, enzymes, metals, polysaccharides, etc and their applications in medicine, food, drug, water treatment, energy and even in aerospace. He specified a special section on what is new in the arena of supports and technologies used in enzyme immobilization and finally a recommendation by the author for future work with a special attention to up-to-date references.

The Use of Microbial Biotechnology in Value Addition to Cassava Wastes as Feed for Pigs: Prospects and Challenges

Cassava peels were fermented through a combination of two lactic acid bacteria (Lactobacillus coryneformis and Lactobacillus delbrueckii) and a fungus (Aspergillus fumigatus) using solid substrate fermentation (SSF) technique for five consecutive days. The enhanced product designated as microbially fermented cassava peel (MFCP) was used at graded levels in the feed formulation for growing pigs. Four iso-nitrogenous and iso-caloric diets were formulated with MFCP at 0% (T1), 20% (T2), 40% (T3) and 60% (T4) inclusion levels. Twenty-four boarlings of an average initial weight of 37.15 kg were used in a fourteen-week trial to test the effect of these graded levels of MFCP on growth performance, digestive and reproductive physiology of the experimental animals. The result showed significant differences (P < 0.05) in mean daily feed consumption. Pigs in T3 had the highest feed intake (2.24 ± 0.05 kg), while pigs in T4 had the lowest (1.78 ± 0.04 kg). The final highest live weight was in T3 (72.00 kg) and the least in T4 (58.17 kg). The feed conversion ratios also showed significant differences (P < 0.05) among treatment means. The result obtained from gut morphometry showed that animals in T1 had the highest stomach weight (full and empty), while animals in T3 had the least rate of gastric emptying (81.50%). Animals in T3 and T4 had the longest small intestine (16.21 m and 15.35 m, respectively), while those in T4 had the longest colon (4.24 m). Animals in T1 and T2 had more numbers of gastric glands, while animals in T3 and T4 had more parietal cells. The reproductive indices revealed that animals in T1 were better in terms of the weight and relative weight of vesicular and Cowper’s gland, while animals in T4 had the highest value of the weight and relative weight of prostate gland. Proximate analyses of the ileal digesta revealed that the rate of nutrient absorption was highest in T3 and T1 and lowest in T4. Histological examination of the testes revealed normal testicular architecture in the control diet. The testes of animals fed 0%, 20% and 40% MFCP diets exhibited normal and intact lumen, Sertoli cells, and germ cells. However, animal fed 60% MFCP diet revealed massive disruption and degeneration of germinal epithelium, and complete erosion of the seminiferous tubules. It could be concluded that feeding pigs at 40% MFCP would enhance feed consumption, nutrient absorption and improve the growth rate of pigs. Using MFCP beyond 40% level on swine diet could compromise gut health through diarrhoeagenic disposition of the digesta in the distal colon and modification of the parietal and chief cells of the gastric glands. The hypertrophy of the prostate gland and vacuolization of the seminiferous tubules of pigs fed the 60% MFCP diet indicated a probable reduced reproductive performance.

Dual-Use Signal Design for Radar and Communication via Joint Orthogonal Signal and Phase Modulation

This paper proposes a new information modulation resorting to orthogonal signal and its phase for dual-function radar communication(DFRC)systems.Focusing on the standardized linear frequency modulation(LFM)signal by additional phase,a bank of signals enjoying satisfactory autocorrelation and cross-correlation characteristics,are generated.Then,these signals map the different information as well as their phases are also modulated to increase the communication bit rate,thus yielding a series of dual-use signals.Finally,the radar detection and communication performance of dual-use signals are also provided through numerical simulation and half-physical platform verification,confirming the effectiveness of the designed signals compared with the existing design strategy.

Single-molecule biotechnology for protein researches

Cells employ proteins to perform metabolic functions and maintain active physiological state through charge transfer and energy conversion.These processes are carried out in a narrow space precisely and rapidly,which,no doubt,bring great difficulty for their detection and dissection.Fortunately,in recent years,the development and expansion of single-molecule technique in protein research make monitoring the dynamical changes of protein at single-molecule level a reality,which also provides a powerful tool for the further exploration of new phenomena and new mechanisms of life activities.This paper aims to summarize the working principle and essential achievements of single-molecule technique in protein research in recent five years.We focus on not only dissecting the difference of nanopores,atomic force microscope,scanning tunneling microscope,and optical tweezers technique,but also discussing the great significance of these single-molecule techniques in investigating intramolecular and intermolecular interactions,electron transport,and conformational changes.Finally,the opportunities and challenges of the single-molecule technique in protein research are discussed,which provide a new door for single-molecule protein research.

SEAWEED CULTIVATION: A NEW APPLIED FIELD FOR BIOTECHNOLOGY

Seaweeds cultivation has resulted in great achievements, although it has a history ofonly a few decades. With higher productivity and resulting higher profit, it has become the leading marineexploitation industry with the brightest prospects. The relatively limited species introduced for commercialcultivation showed great biological diversity. Introduction of selected good strains for tradtional cultivationand the transformation from cultivation of mixed stains to that of pure cell lines are two certain tendenciesin the future. Pure line cultivation of seaweeds is a sort of advanced biotechnology. It provides new op-portunities for not only the industry itself, but also the stable market of high quality natural marine prod-ucts. More work should be done on principles and methods to obtain optimal results for the combinationof pure line cultivation techniques with advanced biochemistry. The Programmed batch production of finechemicals such as polysaccharides and proteins will probably become the social demand.