Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and im...Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and immunomodulatory activity of Duchesnea phenolic fraction (DPF). ELISA and RIA assay were employed to measured the serum concentration of Th1/Th2 cytokines (IL-2, IL-4, IFN-γ and TNF-α). Administration with 0.25 g/kg, 0.5 g/kg and 1 g/kg DPF significantly reduced the tumor weight by 34.37%, 43.89% and 56.28%, respectively, as compared to the tumor control group. Furthermore, the serum level of IL-2, IFN-γ and TNF-α increased and IL-4 level decreased in a dose-dependent manner during DPF treatment, indicating that the antitumor activity of DPF may be associated with the decrease of TNF-α level and restoration of the balance of Th1/Th2 cell responses. These data suggested that DPF, a mixture of plant polyphenols, had potent anticancer activity which was in part accomplished by its immunomodulatory ability.展开更多
Neuraminidase inhibitors(NAIs)are the mainstay antiviral drugs against influenza infection.In this study,a ligand fishing protocol was developed to screen NAIs using neuraminidase immobilized magnetic beads(NA-MB).Aft...Neuraminidase inhibitors(NAIs)are the mainstay antiviral drugs against influenza infection.In this study,a ligand fishing protocol was developed to screen NAIs using neuraminidase immobilized magnetic beads(NA-MB).After verifying the feasibility of NA-MB with an artificial mixture including NA inhibitors and non-inhibitors,the developed ligand fishing protocol was applied to screen NAIs from the crude extracts of Duchesnea indica Andr.Twenty-four NA binding compounds were identified from the normal butanol(n-BuOH)extract of D.indica as potential NAIs by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS)assisted with Compound Structure Identification(CSI):FingerID,including 12 ellagitannins,4 brevifolin derivatives,3 ellagic acid derivatives,and 4 flavonoids.Among them,9 compounds were isolated and tested for in vitro NA inhibitory activities against NA from Clostridium perfringens,and from oseltamivir sensitive and resistant influenza A virus strains.The results indicate that compound B23 has the NA inhibitory activities in both the oseltamivir sensitive and resistant viral NA,with half maximal inhibitory concentration(IC50)values of 197.9 and 125.4μmol/L,respectively.Moreover,B23 can obviously reduce the replication of oseltamivir sensitive and resistant viruses in Madin-Darby canine kidney(MDCK)cells at the concentrations of 40 and 200μmol/L.展开更多
【目的】研究Cd污染对蛇泡草生理生化特性指标的影响,为重金属对中草药的毒害作用研究提供参考。【方法】采用盆栽模拟试验,待蛇泡草幼苗待生长稳定后,以50 m L/d的量浇灌不同浓度(11、22、45、90和134 mg/kg)的Cd2+溶液,记录蛇泡草5、3...【目的】研究Cd污染对蛇泡草生理生化特性指标的影响,为重金属对中草药的毒害作用研究提供参考。【方法】采用盆栽模拟试验,待蛇泡草幼苗待生长稳定后,以50 m L/d的量浇灌不同浓度(11、22、45、90和134 mg/kg)的Cd2+溶液,记录蛇泡草5、30和45 d的生长情况,同时测定其的可溶性总糖含量、总黄酮含量、及抗氧化酶活性。【结果】随Cd溶液浓度的增加,蛇泡草体内Cd含量不断增大,同时蛇泡草叶片、茎部和根部的可溶性总糖、总黄酮、及抗氧化酶活性均呈现出不断降低的趋势。【结论】Cd较易在蛇泡草体内积累,并会破坏植物的新成代谢和正常生长,导致各项生理生化特性指标下降,表现出明显的毒害作用。展开更多
文摘Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and immunomodulatory activity of Duchesnea phenolic fraction (DPF). ELISA and RIA assay were employed to measured the serum concentration of Th1/Th2 cytokines (IL-2, IL-4, IFN-γ and TNF-α). Administration with 0.25 g/kg, 0.5 g/kg and 1 g/kg DPF significantly reduced the tumor weight by 34.37%, 43.89% and 56.28%, respectively, as compared to the tumor control group. Furthermore, the serum level of IL-2, IFN-γ and TNF-α increased and IL-4 level decreased in a dose-dependent manner during DPF treatment, indicating that the antitumor activity of DPF may be associated with the decrease of TNF-α level and restoration of the balance of Th1/Th2 cell responses. These data suggested that DPF, a mixture of plant polyphenols, had potent anticancer activity which was in part accomplished by its immunomodulatory ability.
基金financially supported by National Natural Science Foundation,China(81872830,31970884,and U1801287)the International Science&Technology Cooperation Program of Guangzhou,China(201807010022)National Natural Science Foundation of Guangdong Province,China(No.2019A1515011489)
文摘Neuraminidase inhibitors(NAIs)are the mainstay antiviral drugs against influenza infection.In this study,a ligand fishing protocol was developed to screen NAIs using neuraminidase immobilized magnetic beads(NA-MB).After verifying the feasibility of NA-MB with an artificial mixture including NA inhibitors and non-inhibitors,the developed ligand fishing protocol was applied to screen NAIs from the crude extracts of Duchesnea indica Andr.Twenty-four NA binding compounds were identified from the normal butanol(n-BuOH)extract of D.indica as potential NAIs by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS)assisted with Compound Structure Identification(CSI):FingerID,including 12 ellagitannins,4 brevifolin derivatives,3 ellagic acid derivatives,and 4 flavonoids.Among them,9 compounds were isolated and tested for in vitro NA inhibitory activities against NA from Clostridium perfringens,and from oseltamivir sensitive and resistant influenza A virus strains.The results indicate that compound B23 has the NA inhibitory activities in both the oseltamivir sensitive and resistant viral NA,with half maximal inhibitory concentration(IC50)values of 197.9 and 125.4μmol/L,respectively.Moreover,B23 can obviously reduce the replication of oseltamivir sensitive and resistant viruses in Madin-Darby canine kidney(MDCK)cells at the concentrations of 40 and 200μmol/L.
文摘【目的】研究Cd污染对蛇泡草生理生化特性指标的影响,为重金属对中草药的毒害作用研究提供参考。【方法】采用盆栽模拟试验,待蛇泡草幼苗待生长稳定后,以50 m L/d的量浇灌不同浓度(11、22、45、90和134 mg/kg)的Cd2+溶液,记录蛇泡草5、30和45 d的生长情况,同时测定其的可溶性总糖含量、总黄酮含量、及抗氧化酶活性。【结果】随Cd溶液浓度的增加,蛇泡草体内Cd含量不断增大,同时蛇泡草叶片、茎部和根部的可溶性总糖、总黄酮、及抗氧化酶活性均呈现出不断降低的趋势。【结论】Cd较易在蛇泡草体内积累,并会破坏植物的新成代谢和正常生长,导致各项生理生化特性指标下降,表现出明显的毒害作用。
