期刊文献+
共找到542篇文章
< 1 2 28 >
每页显示 20 50 100
Genetic Variation of the VP1 Gene of the Virulent Duck Hepatitis A Virus Type 1(DHAV-1) Isolates in Shandong Province of China 被引量:13
1
作者 Jiming Gao Junhao Chen +5 位作者 Xingkui Si Zhijing Xie Yanli Zhu Xingxiao Zhang Shujing Wang Shijin Jiang 《Virologica Sinica》 CAS CSCD 2012年第4期248-253,共6页
To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete ... To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete sequence of the virion protein 1(VP1) gene of nine virulent DHAV-1 strains,which were isolated from infected ducklings with clinical symptoms in Shandong province of China in 2007-2008,were tested.The fifth generation duck embryo allantoic liquids of the 9 isolates were tested on 12-day-old duck embryos and on 7-day-old ducklings for the median embryonal lethal doses(ELD 50 s) and the median lethal doses(LD 50 s),respectively.The results showed that the ELD 50 s of embryonic duck eggs of the 9 DHAV-1 isolates were between 1.9 × 10 6 /mL to 1.44 × 10 7 /mL,while the LD 50 s were 2.39 × 10 5 /mL to 6.15 × 10 6 /mL.Cross-neutralization tests revealed that the 9 DHAV-1 isolates were completely neutralized by the standard serum and the hyperimmune sera against the 9 DHAV-1 isolates,respectively.Compared with other virulent,moderate virulent,attenuated vaccine and mild strains,the VP1 genes of the 9 strains shared 89.8%-99.7% similarity at the nucleotide level and 92.4%-99.6% at amino acid level with other DHAV-1 strains.There were three hypervariable regions at the C-terminus(aa 158-160,180-193 and 205-219) and other variable points in VP1 protein,but which didn't cause virulence of DHAV-1 change. 展开更多
关键词 duck hepatitis A virus type 1 (DHAV-1) Embryonal lethal dose (ELDs0) Lethal dose (LDso) Cross-neutralization tests Virionprotein 1 (VP 1)
下载PDF
Replication of hepatitis B virus in primary duck hepatocytes transfected with linear viral DNA 被引量:2
2
作者 Yun-Qing Yao Ding-Feng Zhang +10 位作者 Ni Tang Ai-Long Huang Xiao-Yi Zou Jiang-Feng Xiao Yun Luo Da-Zhi Zhang Bo Wang Wei-Ping Zhou Hong Ren Qi Liu Shu-Hua Guo 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第32期5019-5021,共3页
AIM: To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).METHODS: Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19×... AIM: To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).METHODS: Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19×1012copies of linear HBV DNA/1×107 PDHs). After 1-5 d of transfection, HBsAg and HBeAg in the supernatant and lysate of PDHs were measured with the IMX System.Meanwhile, replicative intermediates of HBV DNA were analyzed by Southern blotting and Dot blotting. PDHs electroporated were used as control group.RESULTS: HBsAg in the hepatocyte lysates of transfected group was 15.24 (1 d), 14.55 (3 d) and 5.13 (5 d; P/N values, positive≥2.1) respectively. HBeAg was negative (<2.1). Both HBsAg and HBeAg were negative in the supernatant of transfected group. Dot blotting revealed that HBV DNA was strongly positive in the transfected group and negative in the control group. Southern blot analysis of intracellular total DNA indicated that there were relaxed circular (rc DNA), covalently closed circular (ccc DNA), and single-stranded (ss DNA) HBV DNA replicative intermediates in the transfected group, there was no integrated HBV DNA in the cellular genome. These parameters were negative in control group.CONCLUSION: Expression and replication of HBV genes can occur in hepatocytes from non-mammalian species.HBV replication has no critical species-specificity, and yet hepatic-specific regulating factors in hepatocytes may be essential for viral replication. 展开更多
关键词 hepatitis B virus REPLICATION EXPRESSION Primary duck hepatocytes
下载PDF
The Effect of Gankang Suppository on Duck Hepatitis B Virus, Serum Biochemistry and Liver Histology in Ducklings 被引量:1
3
作者 李晖 田德英 +2 位作者 吴会玲 陈淼 陈安群 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2008年第4期421-425,共5页
To examine the effect of Gankang Suppository on duck hepatitis B virus (DHBV), the serum biochemistry and hepatic histology in an animal model of DHBV infection, a model of DHBV infection was established by infectin... To examine the effect of Gankang Suppository on duck hepatitis B virus (DHBV), the serum biochemistry and hepatic histology in an animal model of DHBV infection, a model of DHBV infection was established by infecting 1-day-old Yingtaogu ducklings with DHBV-positive serum. The successful model was confirmed by PCR assay and 48 ducklings infected with DHBV were randomly divided into 3 groups: a Gankang Suppository treatment group, an acyclovir (ACV) group and a DHBV model group (control), with each group having 16 animals. All the animals were given the medicines for 4 weeks in a row. The serum of the animals was taken 14 and 28 days after the medica- tion and 7 days after drug discontinuation. Real-time PCR was performed to detect the copy numbers of DHBV DNA in the serum. ALT and AST were dynamically monitored. The ducklings were sacrificed on the 7th day after the discontinuation of the treatment and livers were harvested and examined for inflammation and degeneration of liver cells by using HE staining. The results showed that on day 14, 28 after the treatment and day 7 after the withdrawal, the logarithmic values (log) of DHBV DNA copy numbers in ducklings of Gankang Suppository treatment group were significantly lower than that before the treatment (P=0.0092, P=0.0070, P=0.0080, respectively). Compared with DHBV model control group, the ALT level was significantly decreased (P=0.0020, P=0.0019, respectively) on day 28 after the treatment and on day 7 after the withdrawal. The AST level was also reduced on day 14 after the treatment (P=0.0298). Compared with the ACV control group, the level of ALT was lower on day 7 after the withdrawal (P=0.0016). Histologically, the hepatocyte swelling, vacuolous degeneration and acidophilic degeneration in Gankang Suppository treatment group were alleviated 7 days after the withdrawal as compared with model control group (P=0.0282, P=0.0084, P=0.0195, respectively). It is concluded that Gankang Suppository can effectively suppress DHBV replication, reduce the levels of serum ALT and AST and improve hepatic histology. 展开更多
关键词 duck hepatitis B virus Gankang Suppository duck hepatitis animal model BIOCHEMISTRY HISTOLOGY
下载PDF
Isolation,Identification and Pathogenic Characteristics of Duck Hepatitis Virus Isolates 被引量:1
4
作者 GAO Ya-dong LING Hong-li DONG Rui-e SUN Hai-xin 《Animal Husbandry and Feed Science》 CAS 2010年第8期37-39,共3页
[ Objective] The aim of this study was to determine and analyze the pathogen and the reasons of duck viral hepatitis which is prevalent recently and difficult to control. [Method] Viruses were isolated from livers and... [ Objective] The aim of this study was to determine and analyze the pathogen and the reasons of duck viral hepatitis which is prevalent recently and difficult to control. [Method] Viruses were isolated from livers and spleens of ducks with typical clinical symptoms in Linyi, Weifang, Binzhou and other regions of Shandong Province. The pathogenic characteristics were observed by inoculation in chicken or duck embryo, RT- PCR, serological test, and duck regression. [ Result] Four duck hepatitis virus (DHV) strains were isolated, and the 5th passage allantoic fluid contained 10^3.41 -10^5.20 ELD50/mI. The serum cross protection rate was 20% -80% between the DHV stains and DHV type I. The mortalities of 4- day-old healthy ducks challenged by these four stains were 50% -100%. All challenged ducks had typical lesions of duck viral hepatitis, and the death peak appeared after 24-48 h. [Conclusion] The virulence of different DHV isolates has regional difference. 展开更多
关键词 duck hepatitis virus IDENTIFICATION ISOLATION viruLENCE
下载PDF
Molecular Characterization and SYBR Green I-Based Quantitative PCR for Duck Hepatitis Virus Type 1 被引量:1
5
作者 LUO Yu-jun ZHANG Gui-hong +2 位作者 XU Xiao-qin CHEN Jian-hong LIAO Ming 《Agricultural Sciences in China》 CAS CSCD 2008年第9期1140-1146,共7页
To determine the genomic sequence of a duck hepatitis virus type 1 (DHV-1) strain, real-time quantitative polymerase chain reaction (RTQ-PCR) assay based on SYBR Green I technology was developed to target 3D gene ... To determine the genomic sequence of a duck hepatitis virus type 1 (DHV-1) strain, real-time quantitative polymerase chain reaction (RTQ-PCR) assay based on SYBR Green I technology was developed to target 3D gene of DHV-1, Comparative sequence analysis showed that the genome has a typical picornarivus genetic organization, and strain DHV-1 R genetic organaiztion is 5' untranslated region (UTR)-VP0-VP3-VPI-2A1-2A2-2B-2C-3A-3B-3C-3D-3' UTR, DHV-1 R has close relationship with Parechovirus, and has 95.1-99.1% nucleotide sequence identity with other DHV-1 strains. Based on the DHV-1 sequences in GenBank, three pairs of specific primers were designed to amplify DHV-1 using real-time PCR. The results showed that real-time PCR Tm value is 85.6℃ and the real-time PCR provides a broad dynamic range, detecting from 102 to 109 copies of DHV-1 cDNA per reaction. No cross-reactions were found in specimens containing DPV, AIV and NDV. It is concluded that DHV-1 belongs to a new group of the family Picornaviridae that may form a separate genus most closely related to the genus Parechovirus. All results showed that the real-time PCR has high sensitivity and specificity to detect DHV-1 using SYBR Green I dissociation curve analysis, isolates can be distinguished by their melting temperature. These methods are rapid, sensitive, and reliable, and can be readily adapted for detection of DHV-1 from other clinical samples. 展开更多
关键词 duck hepatitis virus type 1 complete genome real-time RT-PCR
下载PDF
Complete Genomic Sequence of a Chinese Isolate of Duck Hepatitis Virus 被引量:9
6
作者 Guang-qing LIU Fei WANG +4 位作者 Zheng NI Tao YUN Bin YU Jiong-gang HUANG Jian-Ping CHEN 《中国病毒学》 CSCD 2007年第5期353-359,共7页
The complete genomic sequence of Duck hepatitis virus 1(DHV-1) ZJ-V isolate was sequenced and determined to be 7 691 nucleotides(nt) in length with a 5'-terminal un-translated region(UTR) of 626 nt and a 3'-te... The complete genomic sequence of Duck hepatitis virus 1(DHV-1) ZJ-V isolate was sequenced and determined to be 7 691 nucleotides(nt) in length with a 5'-terminal un-translated region(UTR) of 626 nt and a 3'-terminal UTR of 315 nt(not including the poly(A) tail).One large open reading frame(ORF) was found within the genome(nt 627 to 7 373) coding for a polypeptide of 2 249 amino acids.Our data also showed that the poly(A) tail of DHV-1 has at least 22 A's.Sequence comparison revealed significant homology(from 91.9% to 95.7%) between the protein sequences of the ZJ-V isolate and those of 21 reference isolates.Although DHV-1 has been classified as an unassigned virus in the Picornaviridae family,its genome showed some unique characteristics.DHV-1 contains 3 copies of the 2A gene and only 1 copy of the 3B gene,and its 3'-NCR is longer than those of other picornaviruses.Phylogenetic analysis to do sequence homology based on the VP1 protein sequences showed that the ZJ-V isolate shares high sequence homology with the reported DHV-1 isolates(from 92.9% to 99.2%),indicating that DHV-1 is genetically stable. 展开更多
关键词 全基因序列 鸭肝炎病毒 中国 隔离群
下载PDF
The Potential of Duck Hepatitis Virus(DHV-1) Stimulating the Body Weight Gain and the Effects of Silymarin on It in Duckling 被引量:1
7
作者 LIU Wei-min WANG Bing-yun CHEN Jian-hong WANG Jun JI Hui-qin YUAN Sheng HUANG De-chun LI Kang-lin 《Agricultural Sciences in China》 CAS CSCD 2009年第11期1403-1408,共6页
To evaluate the effects of duck hepatitis virus-1 (DHV-1) on the body weight gain in duck and the effects of silymarin on it in vivo, 100 10-d-old ducks, both male and female, were collected to be subjected to the t... To evaluate the effects of duck hepatitis virus-1 (DHV-1) on the body weight gain in duck and the effects of silymarin on it in vivo, 100 10-d-old ducks, both male and female, were collected to be subjected to the test. The experiments were conducted in 8 groups: in group 1-3, the animals were inoculated with 1:105 diluted duck hepatitis virus (DHV-1) infected allantoic fluid and given 0, 30, and 50 mg kg^-1 BW d^-1 silymarin orally, respectively. In group 4-6, the animals were inoculated with 1:5 × 105 diluted DHV-1 infected allantoic fluid and given 0, 10, and 30 mg kg^-1 BW d^-1 silymarin orally, respectively. In group 7, the animals were given 10 mg kg^-1 BW d^-1 silymafin only. Group 8 was the control one treated by injecting sterillized saline into the leg muscles. All the silymarin was given from 0 to 4 d after inoculation of the virus. By the 5th d after inoculation, the vein blood was drawn from the dorsal foot vein and the plasma samples were collected and stored at -20℃. The body weight gain (BWG) was measured from 0 to 10 d after inoculation. The plasma IGF-I, T3, and T4 concentrations were measured by radioimmunoassay (RIA). At the virus dose of 1:5 ×105 diluted virus infected allantoic fluid, the inoculation of the virus enhanced the BWG significantly compared with that of the control (P〈 0.01), while 10-50 mg kg^-1 BW d^-1 silymarin could counteract the effects of the virus on the BWG dose-dependently. The plasma IGF-I levels showed no correlation with the BWG, but the T3 levels showed a same tropism with the body weight gain. The present results indicated that sublethal DHV-1 enhanced the body weight gain of ducklings significantly, and the silymarin could counteract this effect in vivo. 展开更多
关键词 duck hepatitis virus-l DHV body weight gain SILYMARIN INFECTION duckLING
下载PDF
Molecular Characterization of Duck Hepatitis B Virus Isolated from Hubei Brown Ducks
8
作者 胡权 张小勇 +3 位作者 雷延昌 张正茂 Mengji Lu 杨东亮 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第5期633-636,共4页
The objective of this study was to characterize the genome structure of duck hepatitis B virus (DHBV) isolated from Hubei brown ducks. The natural carrier rate of DHBV in adult ducks from Hubei area was investigated... The objective of this study was to characterize the genome structure of duck hepatitis B virus (DHBV) isolated from Hubei brown ducks. The natural carrier rate of DHBV in adult ducks from Hubei area was investigated and the DHBV DNA-positive serum screened out. The complete genome of a DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the carrier rate of DHBV in Hubei brown ducks was 10 % This strain (GenBank accession number DQ276978) had a genome of 3024 nucleotides with three overlapping open reading frames encoding the surface, core and polymerase proteins respectively. Comparison of the strain with 17 DHBV strains registered in GenBank revealed a homology from 89.3 % to 93.5 % at the nucleotide level. The sequences of the structural and functional domains of these proteins were highly conserved. The strain was found to share more signature amino acids in the polymerase genes with the "Chinese" DHBV strains than those of the "Western" country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, the DQ276978 might belong to a subtype of the Chinese DHBV strains. 展开更多
关键词 duck hepatitis B virus homology analysis GENOME CLONING
下载PDF
Development of RT PCR Assay for Detection of Duck Hepatitis Virus Type 1
9
作者 Wang Yongjuan Cui Pingfu +1 位作者 Liu Bo Meng Ting 《Animal Husbandry and Feed Science》 CAS 2017年第5期308-310,共3页
In this study,a RT-PCR assay for rapid detection of duck hepatitis virus type 1( DHV-1) was established in the presence of a pair of primers designed based on vp1 gene sequence,using the genomic RNA of DHV-1 or other ... In this study,a RT-PCR assay for rapid detection of duck hepatitis virus type 1( DHV-1) was established in the presence of a pair of primers designed based on vp1 gene sequence,using the genomic RNA of DHV-1 or other common viruses as the template. The template was serially diluted 10-fold to determine the reaction sensitivity. Finally,seven liver samples of ducks with suspected DHV-1 infection,which were collected from different regions of Jiangsu Province,were detected with this method. The results showed that a DNA fragment of about 360 bp was specifically amplified with the RT-PCR system,and the detection limit was1 fg/μL. The detection rate of clinical samples with this method was 100%. The results revealed that the RT-PCR system which had high specificity and high sensitivity in the detection of DHV-1 was successfully established. 展开更多
关键词 duck hepatitis virus TYPE 1 RT PCR DETECTION
下载PDF
Therapeutic effect of Styela plicata on duck hepatitis B virus in vivo
10
作者 张淼 王瑞 +2 位作者 闫荟 曾凡林 万新祥 《Journal of Medical Colleges of PLA(China)》 CAS 2006年第6期352-357,共6页
Objective:To evaluate the antiviral activity of the alcohol extract of Styela plicata on DHBV (duck hepatitis B virus) in vivo. Methods: Guangzhou-Sheldrake ducklings congenitally infected with DHBV were assigned to r... Objective:To evaluate the antiviral activity of the alcohol extract of Styela plicata on DHBV (duck hepatitis B virus) in vivo. Methods: Guangzhou-Sheldrake ducklings congenitally infected with DHBV were assigned to receive the alcohol extract of Styela plicata or lamivudine for 30 consecutive days. The DHBV DNA of sera was detected by RT-PCR. and the histological analysis of duckling liver was evaluated. Results:Thirty days after therapy,histological analysis of duckling liver showed that the ducklings receiving the alcohol extract of Styela plicata or lamivudine exhibited catabatic status in the degree of liver cell degeneration and inflammation compared with the ducklings receiving normal diet. DHBV DNA of sera from alcohol extract of Styela plicata-treated ducklings and lamivudine-treated ducklings all produced significantly lower levels compared with ducklings receiving normal diet (P<0. 01 ). Although these treatment groups all exhibited a rebound phenomenon 10 d after withdrawal of medication, they still exhibited a significant lower level of serum DHBV DNA compared with the control group responded to normal diet (P<0. 05, P<0. 01). Conclusion:Styela plicata may be an effective antiviral medicine in treating chronic hepatitis B. The data of this experiment will be valuable in studying the therapeutic role and the potential therapeutic mechanism of Styela plicata. 展开更多
关键词 Styela plicata duck hepatitis B virus chronic hepatitis B RT-PCR SYBR Green I dye
下载PDF
DUCK HEPATITIS B VIRUS DNA WITHIN HEPATIC MULTICENTRIC CANCER AND/OR METASTATIC CANCER
11
作者 杨广笑 王全颖 +3 位作者 金友南 迟宝荣 李家敏 叶维法 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1992年第1期9-16,共8页
Duck hepatitis B vims (DHBV) DNA was detected in different tumorous nodules of ducks with hepatic multicentric cancer or intrahepatic metastasis by Southern blot technique. Among 7 ducks with hepatocellular carcinoma ... Duck hepatitis B vims (DHBV) DNA was detected in different tumorous nodules of ducks with hepatic multicentric cancer or intrahepatic metastasis by Southern blot technique. Among 7 ducks with hepatocellular carcinoma of multiple tumor nodules, the hybridization pattern of Integrated DHBV DNA In different tumorous nodules was identical in 3 cases and different in 2 cases. One case showed a similar hybridization pattern in two tumorous nodules and other one was negative tor DHBV DNA. Integrated DHBV DNA was also identified in a metastatic lung cancer of ducks with hepatocellular carcinoma. The hybridization pattern of metastasis of lungs was as the some as that in primary hepatocellular carcinoma. The same discrete hybridization bands In the different tumorous nodules indicate that these nodules might arise from one transformed cell. The different hybridization patterns In various tumorous nodules show that these tumorous nodules might arise from various transformed cells. The results suggest that the hybridization pattern of different nodules of hepatocellular carcinoma with viral DNA probe could make a cell clone origin marker of tumor nodule to differentiate hepatic multlcentric cancer from Intrahepatic metastatic cancer. 展开更多
关键词 DNA duck hepatitis B virus DNA WITHIN hepatIC MULTICENTRIC CANCER AND/OR METASTATIC CANCER DHBV
下载PDF
Detection of the covalently closed circular DNA of duck hepatitis B virus by Taq-Man fluorescent quantitative PCR assay
12
作者 MEI LI FU QING LIN +3 位作者 XIAO PENG LIU SHUI LAN SHI DONG LIANG LI ZI RONG CHEN 《Journal of Microbiology and Immunology》 2007年第1期35-39,共5页
To develop a fluorescent quantitative PCR assay based on Taq-Man chemistry to detect the covalenfly closed circular DNA (eccDNA) of duck hepatitis B virus (DHBV), a pair of primers was designed from both sides of ... To develop a fluorescent quantitative PCR assay based on Taq-Man chemistry to detect the covalenfly closed circular DNA (eccDNA) of duck hepatitis B virus (DHBV), a pair of primers was designed from both sides of the nick in the minus strand of DHBV and a Taq-Man probes between the primers, modified with 6-Fam at 5' end and Tamra at its 3' end was designed to detect the PCR products during PCR cycles. The DHBV DNA fragment was cloned into vector PUCm-T, and the recombinant plasmid was purified and subsequently qualified as the HBV DNA standard. The experimental conditions and reagents used in PCR assay for amplification were sophisticatedly optimized in order to yield a perfect amplification efficacy and reduce the possibility to produce non-specific amplification. It was demonstrated that the detect limit of assay was 10^3 copies/ml, and a linear standard curve was obtained between 10^5 -10^9 copies/ml [ C1 =-2.8361 ln(x) + 41.45, r =-0.9985]. The coefficient of variation was 0.2%-3.14% and 2.22%-4.43% for intra- and inter-assay respectively. After a dynamic survey on the contents of DHBV DNA in serum of ducks, it was found that its peak value appeared at the second week of birth in ducks. It is evident that this method of Taq-Man fluorescent quantitative PCR assay appears to be simple, sensitive and specific. 展开更多
关键词 duck hepatitis B virus Covalently closed circular DNA(cccDNA) Fluorescence quantitative PCR
下载PDF
Is hepatitis B-virucidal validation of biocides possible with the use of surrogates?
13
作者 Andreas Sauerbrei 《World Journal of Gastroenterology》 SCIE CAS 2014年第2期436-444,共9页
The hepatitis B virus(HBV)is considered to be a major public health problem worldwide,and a significant number of reports on nosocomial outbreaks of HBV infections have been reported.Prevention of indirect HBV transmi... The hepatitis B virus(HBV)is considered to be a major public health problem worldwide,and a significant number of reports on nosocomial outbreaks of HBV infections have been reported.Prevention of indirect HBV transmission by contaminated objects is only possible through the use of infection-control principles,including the use of chemical biocides,which are proven to render the virus non-infectious.The virucidal activity of biocides against HBV cannot be predicted;therefore,validation of the virucidal action of disinfectants against HBV is essential.However,feasible HBV infectivity assays have not yet been established.Thus,surrogate models have been proposed for testing the efficacy of biocides against HBV.Most of these assays do not correlate with HBV infectivity.Currently,the most promising and feasible assay is the use of the taxonomically related duck hepatitis B virus(DHBV),which belongs to the same Hepadnaviridae virus family.This paper reviews the application of DHBV,which can be propagated in vitro in primary duck embryonic hepatocytes,for the testing of biocides and describes why this model can be used as reliable method to evaluate disinfectants for efficacy against HBV.The susceptibility levels of important biocides,which are often used as ingredients for commercially available disinfectants,are also described. 展开更多
关键词 hepatitis B virus Surrogate model duck hepatitis B virus DISINFECTANTS Testing virucidal efficacy
下载PDF
SEQUENCE ANALYSIS OF DUCK HEPATITIS B VIRUS GENOME OF THE VARIANT ISOLATED FROM CHINESE DUCKS
14
作者 杨文刚 陈鸿珊 +3 位作者 金奇 胡钢 袁劲松 侯云德 《Chinese Science Bulletin》 SCIE EI CAS 1992年第13期1126-1129,共4页
Duck hepatitis B virus (DHBV) and its prototype human hepatitis B virus (HBV) are members of the hepadnavirus family. They have several characteristics in common, such as viral genomic structure and replication mechan... Duck hepatitis B virus (DHBV) and its prototype human hepatitis B virus (HBV) are members of the hepadnavirus family. They have several characteristics in common, such as viral genomic structure and replication mechanisms. But ducks, the DHBV natural host 展开更多
关键词 duck hepatitis B virus DNA SEQUENCE direct REPEAT sequence.
原文传递
Ⅰ型鸭肝炎病毒逆转录套式PCR检测方法的建立 被引量:16
15
作者 黄显明 张小飞 +2 位作者 李春芬 廖俊伟 毛火云 《中国兽医科学》 CAS CSCD 北大核心 2008年第1期25-28,共4页
根据GenBank中登录的Ⅰ型鸭肝炎病毒(DHV-Ⅰ)A66株的RNA聚合酶基因序列,设计合成了2对引物,建立了适合DHV-Ⅰ快速检测的逆转录套式PCR方法(RT-nested-PCR),采用该方法对DHV-ⅠA66弱毒株和R85952强毒株进行了检测。结果显示,均能扩增到30... 根据GenBank中登录的Ⅰ型鸭肝炎病毒(DHV-Ⅰ)A66株的RNA聚合酶基因序列,设计合成了2对引物,建立了适合DHV-Ⅰ快速检测的逆转录套式PCR方法(RT-nested-PCR),采用该方法对DHV-ⅠA66弱毒株和R85952强毒株进行了检测。结果显示,均能扩增到304 bp的条带,而正常鸭胚、健康鸭肝组织、鸭瘟病毒、鹅细小病毒、禽流感病毒(H9亚型)、新城疫病毒、传染性腔上囊病病毒、减蛋综合征病毒、鸭源大肠杆菌、鸭疫里氏杆菌和鸭源多杀性巴氏杆菌的扩增结果均为阴性。该方法第1次扩增的敏感性是100 pg,第2次扩增的敏感性是1 fg,第2次比第1次扩增的敏感性高105倍。表明,所建立的逆转录套式PCR方法可用于鸭病毒性肝炎(DVH)的临床诊断、病料检测和分子流行病学调查等。 展开更多
关键词 型鸭肝炎病毒 逆转录套式PCR 检测
下载PDF
鸭肝炎病毒Ⅰ型VP3基因的克隆及原核表达 被引量:11
16
作者 刘家森 甘一迪 +5 位作者 姜骞 孟庆文 韩凌霞 司昌德 刘娣 曲连东 《中国兽医科学》 CAS CSCD 北大核心 2008年第7期587-590,共4页
参照鸭肝炎病毒Ⅰ型(DHV-Ⅰ)的基因组序列设计并合成了1对引物。通过RT-PCR方法扩增获得了DHV-Ⅰ病毒161/79/V结构蛋白VP3基因,并将VP3基因片段插入pGEX-6p-1表达载体,转化E.coli Rosetta-gami(DE3)pLysS。SDS-PAGE分析表明,经用0.1mmol... 参照鸭肝炎病毒Ⅰ型(DHV-Ⅰ)的基因组序列设计并合成了1对引物。通过RT-PCR方法扩增获得了DHV-Ⅰ病毒161/79/V结构蛋白VP3基因,并将VP3基因片段插入pGEX-6p-1表达载体,转化E.coli Rosetta-gami(DE3)pLysS。SDS-PAGE分析表明,经用0.1mmol/LIPTG于16℃诱导表达后,菌体裂解产物有特异的、分子质量约52ku的目的蛋白条带。Western-blotting检测表明,表达产物与DHV-Ⅰ阳性血清发生特异性反应,具有良好的反应原性。 展开更多
关键词 鸭肝炎病毒 VP3基因 克隆 原核表达
下载PDF
Ⅰ型鸭病毒性肝炎病毒和新型鸭肝炎病毒鉴别RT-PCR检测方法的建立 被引量:22
17
作者 何冉娅 罗玉均 +3 位作者 孙伟 何逸民 于淼 张桂红 《黑龙江畜牧兽医》 CAS 北大核心 2009年第3期14-16,共3页
根据GenBank中公布的Ⅰ型鸭病毒性肝炎病毒和实验室分离到的Ⅰ型鸭病毒性肝炎ZJ株、Ⅰ型鸭病毒性肝炎R株所测序列(GenBank登录号分别为EU841005和EF585200)以及新型鸭肝炎病毒全基因组序列,应用PrimerPrimier5.0软件,在序列保守区... 根据GenBank中公布的Ⅰ型鸭病毒性肝炎病毒和实验室分离到的Ⅰ型鸭病毒性肝炎ZJ株、Ⅰ型鸭病毒性肝炎R株所测序列(GenBank登录号分别为EU841005和EF585200)以及新型鸭肝炎病毒全基因组序列,应用PrimerPrimier5.0软件,在序列保守区域分别设计了2对鉴别引物,成功建立了Ⅰ型鸭病毒性肝炎病毒和新型鸭肝炎病毒的鉴别RT—PCR检测方法。结果表明:该方法能从Ⅰ型鸭病毒性肝炎病毒中扩增到与预期相符的471bp条带,从广东佛山分离到的新型鸭肝炎病毒FS株中扩增到705bp的条带,设计的2对引物能够特异性地检测出Ⅰ型鸭病毒性肝炎病毒和新型鸭肝炎病毒,特异性好;分别能够检测出模板含量为0.8ng/μL和1.0ng/μL的Ⅰ型鸭病毒性肝炎病毒和新型鸭肝炎病毒。因此,该方法可用于Ⅰ型鸭病毒性肝炎病毒和新型鸭病毒性肝炎临床样品的鉴别诊断和分子流行病学调查。 展开更多
关键词 型鸭病毒性肝炎病毒 新型鸭肝炎病毒 鉴别RT—PCR
下载PDF
Ⅰ型鸭肝炎病毒VP3基因的克隆与原核表达 被引量:8
18
作者 廖俊伟 张小飞 +4 位作者 黄显明 毛火云 尹秀凤 刘大伟 魏建忠 《动物医学进展》 CSCD 北大核心 2009年第7期49-52,共4页
根据GenBank上登录的Ⅰ型鸭肝炎病毒(DHV-Ⅰ)基因组序列,设计一对特异性引物。通过RT-PCR的方法扩增DHV-Ⅰ(A66株)VP3基因并将其5′端起始处稀有密码子同义突变。用限制性内切酶BamHⅠ/SalⅠ消化VP3基因片段和表达载体pGEX-6p-1后构建... 根据GenBank上登录的Ⅰ型鸭肝炎病毒(DHV-Ⅰ)基因组序列,设计一对特异性引物。通过RT-PCR的方法扩增DHV-Ⅰ(A66株)VP3基因并将其5′端起始处稀有密码子同义突变。用限制性内切酶BamHⅠ/SalⅠ消化VP3基因片段和表达载体pGEX-6p-1后构建重组表达质粒pGEX-VP3,转化E.coliBL21(DE3)。经IPTG诱导后,SDS-PAGE分析表明,VP3基因在大肠埃希菌中大量表达,表达产物的分子质量约为52 ku。Western blot检测表明,表达产物能与DHV-Ⅰ阳性血清发生反应,具有反应原性。 展开更多
关键词 型鸭肝炎病毒 VP3基因 克隆与表达
下载PDF
3株鸭肝炎病毒Ⅰ型结构基因VP1的克隆及序列分析 被引量:10
19
作者 甘一迪 刘家森 +6 位作者 姜骞 郭东春 司昌德 孟庆文 韩凌霞 刘娣 曲连东 《畜牧兽医学报》 CAS CSCD 北大核心 2009年第6期952-957,共6页
通过RT-PCR方法扩增鸭肝炎病毒DHV-1:161/79/V、YH、HN株的VP1基因的核苷酸序列。系统发育分析表明,3株病毒与已发表的DHV-1结构基因VP1核苷酸和氨基酸的同源性分别为92.7%~96.9%和95.4%~96.6%,与DHV-1变异株核苷酸和氨基酸序列的相似... 通过RT-PCR方法扩增鸭肝炎病毒DHV-1:161/79/V、YH、HN株的VP1基因的核苷酸序列。系统发育分析表明,3株病毒与已发表的DHV-1结构基因VP1核苷酸和氨基酸的同源性分别为92.7%~96.9%和95.4%~96.6%,与DHV-1变异株核苷酸和氨基酸序列的相似性均分别低于75%和88%,表明3株病毒属于DHV-1,与变异株是不同的血清型。强毒DHV-1:161/79/V的VP1蛋白第49和第183位氨基酸为T和H,弱毒DHV-1:YH、HN为S和Q,推测这2处位点的改变可能与病毒的强弱有关 DHV-1和其变异株的VP1蛋白均没有保守的RGD序列 变异株N-DHV:90D、04G在第50和51位比DHV-1多2个氨基酸(Q和D),在第147和185位各缺失1个氨基酸(E和L),而在变异株DHV:AP-03337、AP-04009、AP-04114、AP-04203的第145和146位比DHV-1多了2个氨基酸(G、G) 不同血清型的鸭肝炎病毒在46-64位、95-149位、180-223位抗原指数差别较大,推测这些位点的改变可能影响病毒的生物学特性。 展开更多
关键词 鸭肝炎病毒(DHV) VP1基因 序列分析
下载PDF
Ⅰ型鸭病毒性肝炎研究进展 被引量:7
20
作者 黄显明 张小飞 +2 位作者 魏建忠 李春芬 廖俊伟 《动物医学进展》 CSCD 2007年第11期78-81,共4页
鸭病毒性肝炎(DVH)是由鸭肝炎病毒(DHV)引起雏鸭一种高度致死、高度传播性的病毒性传染病。该病以肝炎为主要特征,具有高发病率和高病死率。DHV分为Ⅰ型、Ⅱ型和Ⅲ型,3个型之间无交叉保护作用。Ⅰ型DHV呈世界性分布,并常和其他病毒、细... 鸭病毒性肝炎(DVH)是由鸭肝炎病毒(DHV)引起雏鸭一种高度致死、高度传播性的病毒性传染病。该病以肝炎为主要特征,具有高发病率和高病死率。DHV分为Ⅰ型、Ⅱ型和Ⅲ型,3个型之间无交叉保护作用。Ⅰ型DHV呈世界性分布,并常和其他病毒、细菌混合感染,严重影响了养鸭业,造成了巨大的经济损失。文章对Ⅰ型DVH病原学、致病机理、分子生物学诊断技术和防控等方面进行了综述。 展开更多
关键词 型鸭肝炎病毒 致病机理 基因结构 分子生物学诊断 防控
下载PDF
上一页 1 2 28 下一页 到第
使用帮助 返回顶部