[Objective] A mobility measurement and a regeneration assay were conducted to explore the behavioral effects of Cu2＋ on planarian Dugesia japonica. [Method] Dugesia japonica was respectively treated with different co...[Objective] A mobility measurement and a regeneration assay were conducted to explore the behavioral effects of Cu2＋ on planarian Dugesia japonica. [Method] Dugesia japonica was respectively treated with different concentrations of copper solution for 4 and 6 d, and the blank control group was set. The mobility measurement and a regeneration assay of copper ions were determined by mIC50 and rIC50 . [Result] The mobility IC 50 for adult D. japonica at 24, 48, 72 and 96 h were 86, 3.73, 3.47 and 2.61 mg Cu2＋ /L, respectively. The mobility IC50s for newborns was 2.22, 1.64, 0.87 and 0.93 mg Cu2＋ /L, respectively. Although there was no significant difference between 24 and 48 h, and between 72 and 96 h of copper exposure, Cu2＋ decreased newborns locomotor behavior in a concentration-dependent manner at sub-toxic concentrations. The regeneration IC50 of eyespots and auricles for adult D. japonica at 6 d were calculated as 0.76, 0.78 mg Cu2＋ /L, respectively. The number of eyespots and auricles regeneration in planarian D. japonica showed a concentration-dependent manner. Results showed that newborns mobility and adult regeneration assay for D. japonica in a concentration-dependent manner. [Conclusion] The results of the present study suggest that planarians can be regarded as a useful bioindicator species for toxicological studies in the future.展开更多
The appearance of abnormal growths on the planarian, Dugesia dorotocephala, in response to cadmium with and without pre-exposure to L-buthionine-R, S-sulfoximine (BSO) and concurrent exposure to the polychlorinated bi...The appearance of abnormal growths on the planarian, Dugesia dorotocephala, in response to cadmium with and without pre-exposure to L-buthionine-R, S-sulfoximine (BSO) and concurrent exposure to the polychlorinated biphenyls (PCBs) Aroclor 1254, PCB 28, PCB 110 or PCB 126 is described. Pigmented rose thorn (PRT) lesions were non-invasive and appeared in response to PCBs. Post-head (PH) lesions developed in up to 100% of the animals within 6-20 days post-dosing, progressed rather rapidly and were highly invasive. Round tail tip (RTT) lesions appeared in lower frequencies within 10-30 days, but progressed extremely rapidly resulting in tail loss within 48 h. We have referred to these types of lesions as 'tumors', but they are not necessarily characteristic of vertebrate neoplasms.PCBs interacted with cadmium in a complex way, in some cases increasing total lesions and decreasing time-to-lesion and in other cases having the opposite effects. A three-factor (PCB, PCB dose, Cd dose) nested analysis of variance model was used to determine lesion rates in order to compare PCB potencies as potentiators or antagonists. The Aroclor mixture was always the least potent co-toxicant but appeared to be the most potent antagonist; the coplanar PCB 126 was the most potent co-toxicant. The complex response surfaces and the lack of stoichiometry in dose-response relationships indicate that multiple mechanisms are responsible for PH and RTT lesions in planarians. These results emphasize the complexity of PCB toxicities and suggest further studies to validate the planarian model as a screen for combinations or environmental mixtures which may have altered biological potency in other species.展开更多
基金Supported by Natural Science Foundation of Shandong Province(ZR2009BM017,ZR2009DM029)National Natural Science Foundation of China(31172074)~~
文摘[Objective] A mobility measurement and a regeneration assay were conducted to explore the behavioral effects of Cu2＋ on planarian Dugesia japonica. [Method] Dugesia japonica was respectively treated with different concentrations of copper solution for 4 and 6 d, and the blank control group was set. The mobility measurement and a regeneration assay of copper ions were determined by mIC50 and rIC50 . [Result] The mobility IC 50 for adult D. japonica at 24, 48, 72 and 96 h were 86, 3.73, 3.47 and 2.61 mg Cu2＋ /L, respectively. The mobility IC50s for newborns was 2.22, 1.64, 0.87 and 0.93 mg Cu2＋ /L, respectively. Although there was no significant difference between 24 and 48 h, and between 72 and 96 h of copper exposure, Cu2＋ decreased newborns locomotor behavior in a concentration-dependent manner at sub-toxic concentrations. The regeneration IC50 of eyespots and auricles for adult D. japonica at 6 d were calculated as 0.76, 0.78 mg Cu2＋ /L, respectively. The number of eyespots and auricles regeneration in planarian D. japonica showed a concentration-dependent manner. Results showed that newborns mobility and adult regeneration assay for D. japonica in a concentration-dependent manner. [Conclusion] The results of the present study suggest that planarians can be regarded as a useful bioindicator species for toxicological studies in the future.
文摘The appearance of abnormal growths on the planarian, Dugesia dorotocephala, in response to cadmium with and without pre-exposure to L-buthionine-R, S-sulfoximine (BSO) and concurrent exposure to the polychlorinated biphenyls (PCBs) Aroclor 1254, PCB 28, PCB 110 or PCB 126 is described. Pigmented rose thorn (PRT) lesions were non-invasive and appeared in response to PCBs. Post-head (PH) lesions developed in up to 100% of the animals within 6-20 days post-dosing, progressed rather rapidly and were highly invasive. Round tail tip (RTT) lesions appeared in lower frequencies within 10-30 days, but progressed extremely rapidly resulting in tail loss within 48 h. We have referred to these types of lesions as 'tumors', but they are not necessarily characteristic of vertebrate neoplasms.PCBs interacted with cadmium in a complex way, in some cases increasing total lesions and decreasing time-to-lesion and in other cases having the opposite effects. A three-factor (PCB, PCB dose, Cd dose) nested analysis of variance model was used to determine lesion rates in order to compare PCB potencies as potentiators or antagonists. The Aroclor mixture was always the least potent co-toxicant but appeared to be the most potent antagonist; the coplanar PCB 126 was the most potent co-toxicant. The complex response surfaces and the lack of stoichiometry in dose-response relationships indicate that multiple mechanisms are responsible for PH and RTT lesions in planarians. These results emphasize the complexity of PCB toxicities and suggest further studies to validate the planarian model as a screen for combinations or environmental mixtures which may have altered biological potency in other species.
