Halophilic alga Dunaliella bardawil (Chlorophyceae) was cultivated in artificialseawater (containing 3 .0, 1. 5 or 0. 5 mol/L of NaCl respectively) for at least two weeks; total RNAswere then extracted and 8 stable di...Halophilic alga Dunaliella bardawil (Chlorophyceae) was cultivated in artificialseawater (containing 3 .0, 1. 5 or 0. 5 mol/L of NaCl respectively) for at least two weeks; total RNAswere then extracted and 8 stable differential bands were harvested after DDRT-PCR and electrophoresis.The retrieved bands were amplified, subjected to electrophoresis again, and cloned into plasmid pUCm-Trespectively. After exelusion of false positive bands, We obtained a recombinant plasmid pTE containing afragment of cDNA, which was only specilically expressed under high salinity condition. Sequencing of展开更多
Toxic pollutants are metabolic poisons that can seriously injure or destroy the photosynthetic organisms upon which the food chain depends. Since microalgae play a key role in marine ecosystems, marine microalgae are ...Toxic pollutants are metabolic poisons that can seriously injure or destroy the photosynthetic organisms upon which the food chain depends. Since microalgae play a key role in marine ecosystems, marine microalgae are proposed as excellent bio-indicators of pollution due to their high sensitivity, which can give warning of the toxic effects of chemicals sooner than any other species. The aim of this work concentrated on the effect of different concentrations of the antifouling biocide (Irgarol 1051) on growth and chlorophylls content (as an essential metabolite) of the two marine unicellular green algae Chlorella salina and Dunaliella bardawil that usually used in fish feeding. The growth of the wall-less Dunaliella bardawil was more sensitive to Irgarol 1051 than the walled cells Chlorella salina, although the concentrations used were greatly different. The product of photosynthesis in the two algal species greatly affected since in the presence of Irgarol 1051, a serious destructive effect was observed. The cell wall appeared to play a significant role in protecting the organism against toxicity of the antifouling agent either by adsorption or degradation. The strength of toxicity depends mainly on the concentration of the antifouling agent, the length of culturing period and the type of organism tested.展开更多
基金This work was supported by the Foundation of National Science and Technology Ministry,Grant No.J00-B-014,and Science and Technology Project of Xiamen City,Grant No.350222000104
文摘Halophilic alga Dunaliella bardawil (Chlorophyceae) was cultivated in artificialseawater (containing 3 .0, 1. 5 or 0. 5 mol/L of NaCl respectively) for at least two weeks; total RNAswere then extracted and 8 stable differential bands were harvested after DDRT-PCR and electrophoresis.The retrieved bands were amplified, subjected to electrophoresis again, and cloned into plasmid pUCm-Trespectively. After exelusion of false positive bands, We obtained a recombinant plasmid pTE containing afragment of cDNA, which was only specilically expressed under high salinity condition. Sequencing of
文摘Toxic pollutants are metabolic poisons that can seriously injure or destroy the photosynthetic organisms upon which the food chain depends. Since microalgae play a key role in marine ecosystems, marine microalgae are proposed as excellent bio-indicators of pollution due to their high sensitivity, which can give warning of the toxic effects of chemicals sooner than any other species. The aim of this work concentrated on the effect of different concentrations of the antifouling biocide (Irgarol 1051) on growth and chlorophylls content (as an essential metabolite) of the two marine unicellular green algae Chlorella salina and Dunaliella bardawil that usually used in fish feeding. The growth of the wall-less Dunaliella bardawil was more sensitive to Irgarol 1051 than the walled cells Chlorella salina, although the concentrations used were greatly different. The product of photosynthesis in the two algal species greatly affected since in the presence of Irgarol 1051, a serious destructive effect was observed. The cell wall appeared to play a significant role in protecting the organism against toxicity of the antifouling agent either by adsorption or degradation. The strength of toxicity depends mainly on the concentration of the antifouling agent, the length of culturing period and the type of organism tested.
