Dwarf mutants are the crucial resources for molecular biology research and rice breeding. Here, a rice mutant, dwarf and deformed flower3（ddf3）, was identified in tissue culture of Oryza sativa cv. Dongjin. Compared...Dwarf mutants are the crucial resources for molecular biology research and rice breeding. Here, a rice mutant, dwarf and deformed flower3（ddf3）, was identified in tissue culture of Oryza sativa cv. Dongjin. Compared with wild type, the ddf3 mutant exhibited severe dwarfism, a greater number of tillers and significantly decreased fertility. In addition, leaf length, panicle length, and grain length, were significantly shorter. All internodes of ddf3 were shorter than those of wild type, and histological analysis revealed that internode cell elongation was significantly inhibited in ddf3. In the ddf3 mutant, pollen activity was significantly decreased, and the development of most stigmas was abnormal. Genetic analysis indicated that the ddf3 mutant phenotypes are controlled by a single or tightly linked nuclear genes. Using an F2 mapping population generated from a cross between ddf3 and Yangdao 6（9311）, the DDF3 gene was mapped to a 45.21-kb region between insertion-deletion（In Del） markers M15 and M16 on the long arm of chromosome 7. Sequencing revealed a 13.98-kbdeletion in this region in the ddf3 mutant genome that resulted in the complete or partial deletion of ZF（DHHC type zinc finger protein）, EP（expressed protein）, and FH2（actin-binding FH2 domain-containing protein） genes. Quantitative RT-PCR analyses revealed that in wild type, the transcript levels of FH2 were almost the same in all organs, while ZF was mainly expressed in the panicle, and no expression of EP was detected in any organ. Based on these results, ZF and FH2 could be potential DDF3 candidate genes involved in the regulation of rice morphology and flower organ development. Our work has laid the foundation for future functional analysis of these candidate genes and has provided a profitable gene resource for rice breeding for increased fertility in the future.展开更多
在温室生长的条件下,以一年生中国矮樱桃品种‘莱阳’幼苗(Prunus pseudocerasus L. cv. Laiyang)为试材,研究了中国矮樱桃叶片生长和光合作用对不同光照环境(100%、70%、48%、30%、11%)的适应性调节。结果表明,随着光照强度减弱,樱桃...在温室生长的条件下,以一年生中国矮樱桃品种‘莱阳’幼苗(Prunus pseudocerasus L. cv. Laiyang)为试材,研究了中国矮樱桃叶片生长和光合作用对不同光照环境(100%、70%、48%、30%、11%)的适应性调节。结果表明,随着光照强度减弱,樱桃叶面积变大,比叶重下降,叶片变薄,栅栏组织与海绵组织的比值上升。弱光处理还降低了樱桃叶片净光合速率、叶片光饱和点和光补偿点、叶绿素a/b比值,同时提高了叶片可溶性蛋白和叶绿素含量。展开更多
对2007、2008两年在适宜密度下半矮蔓（1．8株／m^2）和矮蔓（2．4株／m^2）各2个西葫芦品种的冠层结构指标与产量进行了研究，结果表明，日光温室中，不同矮蔓型西葫芦品种的冠层结构不同，半矮蔓品种的冠层高度是矮蔓品种的1．63倍，...对2007、2008两年在适宜密度下半矮蔓（1．8株／m^2）和矮蔓（2．4株／m^2）各2个西葫芦品种的冠层结构指标与产量进行了研究，结果表明，日光温室中，不同矮蔓型西葫芦品种的冠层结构不同，半矮蔓品种的冠层高度是矮蔓品种的1．63倍，最大叶面积指数较矮蔓品种高1．06，而叶面积密度较矮蔓品种低1．25m^-1；半矮蔓品种的叶片较上举，功能叶片与主茎夹角在45°～65°之间，矮蔓品种的叶片比较平展，功能叶与主茎夹角在65°～90°之间，结果期平均叶倾角（mean tilt angle，MTA）半矮蔓品种大于矮蔓品种；半矮蔓品种冠层的整体受光态势良好，消光系数平均为0．67，矮蔓品种为0．82。半矮蔓品种与矮蔓品种早熟性上差异不显著，但总产量显著高于矮蔓品种，平均增产24．3％，单株产量的显著提高是其增产的主要原因。产量与结果中期的冠层结构指标相关性最大，其次是结果后期，与苗期相关性最小，结果期维持较高较稳的冠层高度、叶面积指数（1eaf area index，IAI），较低的叶面积密度，有利于西葫芦产量的形成。展开更多
基金supported by the National Natural Science Foundation of China (31560350 and 31760350)the Science and Technology Program of Jiangxi, China (20171ACF60018)
文摘Dwarf mutants are the crucial resources for molecular biology research and rice breeding. Here, a rice mutant, dwarf and deformed flower3（ddf3）, was identified in tissue culture of Oryza sativa cv. Dongjin. Compared with wild type, the ddf3 mutant exhibited severe dwarfism, a greater number of tillers and significantly decreased fertility. In addition, leaf length, panicle length, and grain length, were significantly shorter. All internodes of ddf3 were shorter than those of wild type, and histological analysis revealed that internode cell elongation was significantly inhibited in ddf3. In the ddf3 mutant, pollen activity was significantly decreased, and the development of most stigmas was abnormal. Genetic analysis indicated that the ddf3 mutant phenotypes are controlled by a single or tightly linked nuclear genes. Using an F2 mapping population generated from a cross between ddf3 and Yangdao 6（9311）, the DDF3 gene was mapped to a 45.21-kb region between insertion-deletion（In Del） markers M15 and M16 on the long arm of chromosome 7. Sequencing revealed a 13.98-kbdeletion in this region in the ddf3 mutant genome that resulted in the complete or partial deletion of ZF（DHHC type zinc finger protein）, EP（expressed protein）, and FH2（actin-binding FH2 domain-containing protein） genes. Quantitative RT-PCR analyses revealed that in wild type, the transcript levels of FH2 were almost the same in all organs, while ZF was mainly expressed in the panicle, and no expression of EP was detected in any organ. Based on these results, ZF and FH2 could be potential DDF3 candidate genes involved in the regulation of rice morphology and flower organ development. Our work has laid the foundation for future functional analysis of these candidate genes and has provided a profitable gene resource for rice breeding for increased fertility in the future.
文摘在温室生长的条件下,以一年生中国矮樱桃品种‘莱阳’幼苗(Prunus pseudocerasus L. cv. Laiyang)为试材,研究了中国矮樱桃叶片生长和光合作用对不同光照环境(100%、70%、48%、30%、11%)的适应性调节。结果表明,随着光照强度减弱,樱桃叶面积变大,比叶重下降,叶片变薄,栅栏组织与海绵组织的比值上升。弱光处理还降低了樱桃叶片净光合速率、叶片光饱和点和光补偿点、叶绿素a/b比值,同时提高了叶片可溶性蛋白和叶绿素含量。
文摘对2007、2008两年在适宜密度下半矮蔓（1．8株／m^2）和矮蔓（2．4株／m^2）各2个西葫芦品种的冠层结构指标与产量进行了研究，结果表明，日光温室中，不同矮蔓型西葫芦品种的冠层结构不同，半矮蔓品种的冠层高度是矮蔓品种的1．63倍，最大叶面积指数较矮蔓品种高1．06，而叶面积密度较矮蔓品种低1．25m^-1；半矮蔓品种的叶片较上举，功能叶片与主茎夹角在45°～65°之间，矮蔓品种的叶片比较平展，功能叶与主茎夹角在65°～90°之间，结果期平均叶倾角（mean tilt angle，MTA）半矮蔓品种大于矮蔓品种；半矮蔓品种冠层的整体受光态势良好，消光系数平均为0．67，矮蔓品种为0．82。半矮蔓品种与矮蔓品种早熟性上差异不显著，但总产量显著高于矮蔓品种，平均增产24．3％，单株产量的显著提高是其增产的主要原因。产量与结果中期的冠层结构指标相关性最大，其次是结果后期，与苗期相关性最小，结果期维持较高较稳的冠层高度、叶面积指数（1eaf area index，IAI），较低的叶面积密度，有利于西葫芦产量的形成。
