Objective To evaluate the etiology of Shiga-like toxin-producing Escherichia coli (SLTEC) in children with diarrhea. Methods We designed and synthesized 3 pairs of primers located in the SLT1, SLT2, and eaeA genes of ...Objective To evaluate the etiology of Shiga-like toxin-producing Escherichia coli (SLTEC) in children with diarrhea. Methods We designed and synthesized 3 pairs of primers located in the SLT1, SLT2, and eaeA genes of enterohaemorrhagic Escherichia coli (EHEC), while the virulent genes SLT1, SLT2, and eaeA from E.coli species were amplified by polymerase chain reaction (PCR). Results One strain of EHEC with SLT1, SLT2, and eaeA in 29 reference strains of diarrhea-causing E.coli (DCEC) and 10 strains of other enterobacteria detected by PCR had positive reactions, while all other DCEC and enterobacteria were negative. Of 474 strains of E. coli isolated from 1032 children with diarrhea and detected by PCR, 20 strains of SLT1 producing E. coli (4.2%) positive, and 7 strains of SLT2 producing E.coli (1.5%) positive; while of 74 strains of entero-SLTs-producing and invasive Escherichia coli (ESIEC), 15 strains of SLT1 (20.3%) and 5 strains of SLT2 (6.8%) were positive. Conclusion Shiga-like toxin E. coli has been identified as a major etiologic agent of children with diarrhea in Taiyuan, China.展开更多
Objective： To observe the clinical effects of a modified Tuina method in the treatment of childhood diarrhea. Methods： Thirty children with diarrhea of spleen deficiency were treated by ＂Four-Step Tuina＂（treatmen...Objective： To observe the clinical effects of a modified Tuina method in the treatment of childhood diarrhea. Methods： Thirty children with diarrhea of spleen deficiency were treated by ＂Four-Step Tuina＂（treatment group）; and control group was treated with routine Tuina methods. The scale of children diarrhea was used as an objective index for therapeutic effect evaluation. Results： After treatment, the scale was 1.4±1.92 in treatment group and 3.3±3.45 in control group; the statistical analysis showed that there was a difference between the scales; the treatment group had better effects than control group, in another word, the ＂Four-Step Tuina ＂ was quite effective for children diarrhea. Conclusion： Tuina has positive effects on children diarrhea of spleen deficiency, which provides evidences for the standardization of clinical therapeutic effects.展开更多
Objective:To observe the clinical efficacy of umbilical therapy with herbal cakes made of Ge Gen Qin Lian Tang prescription of different dosages in treating damp-heat diarrhea in young children.Methods:Seventy-two kid...Objective:To observe the clinical efficacy of umbilical therapy with herbal cakes made of Ge Gen Qin Lian Tang prescription of different dosages in treating damp-heat diarrhea in young children.Methods:Seventy-two kids with diarrhea of damp-heat pattern were recruited and divided into a high-dosage group and a low-dosage group using the random number table method,with 36 cases in each group.They all received conventional antidiarrheal treatment and umbilical application with herbal cakes.However,the herbal cakes for the high-dosage group were made of the mixture of Ge Gen Qin Lian Tang powder and water,and those for the low-dosage group consisted of 10%Ge Gen Qin Lian Tang powder and 90%auxiliary materials(corn starch)plus water.The treatment duration was 3 d.The clinical efficacy,antidiarrheal rate,effective rate for symptoms and signs of traditional Chinese medicine(TCM),TCM symptoms score,and safety indicators were compared between the two groups.Results:After the treatment,the TCM symptoms scores dropped significantly in both groups(P<0.01)and were lower in the high-dosage group than in the low-dosage group(P<0.05).The clinical efficacy was more significant in the high-dosage group than in the low-dosage group after the treatment,and the between-group difference was statistically significant(P<0.05);the antidiarrheal rate was markedly higher in the high-dosage group than in the low-dosage group(P<0.05).Regarding the TCM symptoms and signs,the high-dosage group showed better results in improving the greasy and yellowish tongue coating,bowel movement frequency,watery excrement,short and dark urine,red tongue body,red anus,vomiting,bowel sounds,and abdominal bloating compared with the low-dosage group,and the between-group differences were statistically significant(P<0.01).Conclusion:Umbilical therapy with herbal cakes made of Ge Gen Qin Lian Tang is safe,reliable,and effective in treating damp-heat diarrhea in young children;the high-dosage herbal cakes produce more significant efficacy than the low-dosage ones and are worth further investigation.展开更多
Objective: To evaluate the suitability of educational videos produced in Brazil that addressed aspects related to prevention and/or management of infant diarrhea according to content;language;graphic illustrations;sti...Objective: To evaluate the suitability of educational videos produced in Brazil that addressed aspects related to prevention and/or management of infant diarrhea according to content;language;graphic illustrations;stimulation for learning/motivation and cultural fit. Method: Documentary study, which evaluated six educational videos from an adapted and validated tool, the Suitability Assessment of Materials. Three nurses evaluated the videos. Data were processed using the Statistical Package for the Social Sciences, version 17.0, and presented in tables and graphics. Results: It was found that 4 (66.6%) of the educational videos were classified as appropriate, ranging from 60% to 63.3% of full approval, and 2 (33.3%) were assessed as higher, reaching up to 83.3% to 90% approval. Considering the socioeconomic and cultural contexts of the videos to the audience, they assigned notes ranging from 6 to 9 in a rating ranging from 0, the minimum score, to 10, the highest score. Conclusion: The educational videos were considered adequate, with positive results about the content on the prevention and management of infant diarrhea, being appropriate for the target audience. As a result, greater credibility is ratified as the use thereof, since these resources can contribute to health promotion and prevention of diarrhea in children.展开更多
文摘Objective To evaluate the etiology of Shiga-like toxin-producing Escherichia coli (SLTEC) in children with diarrhea. Methods We designed and synthesized 3 pairs of primers located in the SLT1, SLT2, and eaeA genes of enterohaemorrhagic Escherichia coli (EHEC), while the virulent genes SLT1, SLT2, and eaeA from E.coli species were amplified by polymerase chain reaction (PCR). Results One strain of EHEC with SLT1, SLT2, and eaeA in 29 reference strains of diarrhea-causing E.coli (DCEC) and 10 strains of other enterobacteria detected by PCR had positive reactions, while all other DCEC and enterobacteria were negative. Of 474 strains of E. coli isolated from 1032 children with diarrhea and detected by PCR, 20 strains of SLT1 producing E. coli (4.2%) positive, and 7 strains of SLT2 producing E.coli (1.5%) positive; while of 74 strains of entero-SLTs-producing and invasive Escherichia coli (ESIEC), 15 strains of SLT1 (20.3%) and 5 strains of SLT2 (6.8%) were positive. Conclusion Shiga-like toxin E. coli has been identified as a major etiologic agent of children with diarrhea in Taiyuan, China.
文摘Objective： To observe the clinical effects of a modified Tuina method in the treatment of childhood diarrhea. Methods： Thirty children with diarrhea of spleen deficiency were treated by ＂Four-Step Tuina＂（treatment group）; and control group was treated with routine Tuina methods. The scale of children diarrhea was used as an objective index for therapeutic effect evaluation. Results： After treatment, the scale was 1.4±1.92 in treatment group and 3.3±3.45 in control group; the statistical analysis showed that there was a difference between the scales; the treatment group had better effects than control group, in another word, the ＂Four-Step Tuina ＂ was quite effective for children diarrhea. Conclusion： Tuina has positive effects on children diarrhea of spleen deficiency, which provides evidences for the standardization of clinical therapeutic effects.
文摘Objective:To observe the clinical efficacy of umbilical therapy with herbal cakes made of Ge Gen Qin Lian Tang prescription of different dosages in treating damp-heat diarrhea in young children.Methods:Seventy-two kids with diarrhea of damp-heat pattern were recruited and divided into a high-dosage group and a low-dosage group using the random number table method,with 36 cases in each group.They all received conventional antidiarrheal treatment and umbilical application with herbal cakes.However,the herbal cakes for the high-dosage group were made of the mixture of Ge Gen Qin Lian Tang powder and water,and those for the low-dosage group consisted of 10%Ge Gen Qin Lian Tang powder and 90%auxiliary materials(corn starch)plus water.The treatment duration was 3 d.The clinical efficacy,antidiarrheal rate,effective rate for symptoms and signs of traditional Chinese medicine(TCM),TCM symptoms score,and safety indicators were compared between the two groups.Results:After the treatment,the TCM symptoms scores dropped significantly in both groups(P<0.01)and were lower in the high-dosage group than in the low-dosage group(P<0.05).The clinical efficacy was more significant in the high-dosage group than in the low-dosage group after the treatment,and the between-group difference was statistically significant(P<0.05);the antidiarrheal rate was markedly higher in the high-dosage group than in the low-dosage group(P<0.05).Regarding the TCM symptoms and signs,the high-dosage group showed better results in improving the greasy and yellowish tongue coating,bowel movement frequency,watery excrement,short and dark urine,red tongue body,red anus,vomiting,bowel sounds,and abdominal bloating compared with the low-dosage group,and the between-group differences were statistically significant(P<0.01).Conclusion:Umbilical therapy with herbal cakes made of Ge Gen Qin Lian Tang is safe,reliable,and effective in treating damp-heat diarrhea in young children;the high-dosage herbal cakes produce more significant efficacy than the low-dosage ones and are worth further investigation.
文摘Objective: To evaluate the suitability of educational videos produced in Brazil that addressed aspects related to prevention and/or management of infant diarrhea according to content;language;graphic illustrations;stimulation for learning/motivation and cultural fit. Method: Documentary study, which evaluated six educational videos from an adapted and validated tool, the Suitability Assessment of Materials. Three nurses evaluated the videos. Data were processed using the Statistical Package for the Social Sciences, version 17.0, and presented in tables and graphics. Results: It was found that 4 (66.6%) of the educational videos were classified as appropriate, ranging from 60% to 63.3% of full approval, and 2 (33.3%) were assessed as higher, reaching up to 83.3% to 90% approval. Considering the socioeconomic and cultural contexts of the videos to the audience, they assigned notes ranging from 6 to 9 in a rating ranging from 0, the minimum score, to 10, the highest score. Conclusion: The educational videos were considered adequate, with positive results about the content on the prevention and management of infant diarrhea, being appropriate for the target audience. As a result, greater credibility is ratified as the use thereof, since these resources can contribute to health promotion and prevention of diarrhea in children.
