BACKGROUND:p130Cas(p130Crk-associated substance) is a junction protein that is important to the adhesion between cytoskeleton and extracellular matrix.Also the adhesion molecules E-cadherin andβ-catenin play importan...BACKGROUND:p130Cas(p130Crk-associated substance) is a junction protein that is important to the adhesion between cytoskeleton and extracellular matrix.Also the adhesion molecules E-cadherin andβ-catenin play important roles in the invasiveness of carcinoma.This study was undertaken to investigate the effects of p130Cas E-cadherin andβ-catenin on the invasion,metastasis and prognosis of hepatocellular carcinoma(HCC). METHODS:Immunohistochemistry was used to evaluate the expression of p130Cas,E-cadherin,andβ-catenin in 40 patients with HCC.All patients were followed up postoperatively,and the relationship between expression and clinicopathological prognostic parameters was analyzed RESULTS:The positive expression rates of p130Cas and E-cadherin in HCC tissue(n=40)were 62.50%and 55.00% but in normal liver tissue 10%,and 100%,respectively (P<0.05).The abnormal expression rate ofβ-catenin in HCC tissue was 70%,while in normal liver tissue it was 13.33%(P<0.05).The positive rate of p130Cas was correlated with lymph node invasion,pathological stage TNM stage,and a worse prognosis,but not with gender age,HBV infection,hepatic cirrhosis,alpha-fetoprotein (AFP)level before operation,and tumor diameter Similarly,the expression of E-cadherin andβ-catenin was correlated with lymph node invasion,pathological stage,TNM stage,and worse prognosis,but not with gender,age,HBV infection,hepatic cirrhosis,AFP level before operation,and tumor size.Correlations were found between p130Cas and abnormal E-cadherin/β-catenin expression(P<0.001 and<0.05,respectively).CONCLUSIONS:In HCC,there is a negative correlation between the positive expression of p130Cas and the normal expression of the adhesion molecules E-cadherin/β-catenin, and p130Cas plays important roles in the invasion, metastasis and prognosis of HCC.p130Cas may be involved in alterating the structure and function of E-cadherin/ β-catenin,by regulating tyrosine phosphorylation via the p130Cas-Src signal pathway.展开更多
[Objectives] To observe the effects of oxymatrine on the proliferation,invasion and migration of hepatocellular carcinoma cells,and to explore its possible molecular mechanism.[Methods]The hepatocellular carcinoma cel...[Objectives] To observe the effects of oxymatrine on the proliferation,invasion and migration of hepatocellular carcinoma cells,and to explore its possible molecular mechanism.[Methods]The hepatocellular carcinoma cell line Hep G2 was randomly divided into the negative control group and the low,medium and high concentration groups of oxymatrine.The negative control group was added to the cell culture medium,and the low,medium and high concentration groups of oxymatrine were added to the oxymatrine and cell culture medium with the final concentration of 1.0,2.0 and 4.0 mg/m L.The proliferation of each group was measured by MTT assay,and the inhibition rate of cell proliferation was calculated.The invasion and migration ability of each group was determined using Transwell chamber assay.The expression of E-cadherin and CD44 mRNA in each group was detected using Real-time PCR,while the expression of E-cadherin and CD44 protein was detected using Western blotting method.[Results] The inhibition rate of cell proliferation in the high concentration group of oxymatrine was higher than that in the medium and low concentration groups,and the inhibition rate of cell proliferation of medium concentration group was higher than that in the low concentration group.In the cell invasion and cell migration experiments,the number of transmembrane cells of low,medium and high concentration groups of oxymatrine was less than that in the negative control group(P < 0.05),the number of transmembrane cells of high concentration groups of oxymatrine was less than that in medium and low concentration groups,and the number of transmembrane cells of medium concentration group was less than in that of the low concentration group(P < 0.05).Compared with the negative control group,the expression of E-cadherin mRNA and protein increased in the low,medium and high concentration groups of oxymatrine,while the expression of CD44 mRNA and protein decreased(P < 0.05).[Conclusions] Oxymatrine has the effect of inhibiting the proliferation,invasion and migration of hepatocellular carcinoma cell line Hep G2 in vitro.The higher the concentration,the more significant the effect will be.The mechanism is possibly connected with the up-regulation of E-cadherin expression and down-regulation of CD44 expression.展开更多
目的:探讨大肠癌组织中埃兹蛋白(Ezrin),黏着斑激酶(FAK)及上皮细胞钙黏蛋白(E-cadherin)的表达及其与肿瘤侵袭和转移的关系.方法:大肠癌组织标本50例,其中高分化腺癌13例,中低分化腺癌37例;无淋巴结转移30例,淋巴结转移20例.采用免疫...目的:探讨大肠癌组织中埃兹蛋白(Ezrin),黏着斑激酶(FAK)及上皮细胞钙黏蛋白(E-cadherin)的表达及其与肿瘤侵袭和转移的关系.方法:大肠癌组织标本50例,其中高分化腺癌13例,中低分化腺癌37例;无淋巴结转移30例,淋巴结转移20例.采用免疫组织化学方法检测50例大肠癌组织中Ezrin,FAK及E-cadherin的蛋白表达,并运用统计学方法分析Ezrin,FAK及E-cadherin的表达与大肠癌各种临床病理特征的关系及三者的相关性.结果:Ezrin在中低分化、伴有淋巴结转移及Dukes C+D期大肠癌的阳性表达率显著高于高分化、无淋巴结转移及Dukes A+B期大肠癌(83.78% vs 46.15%,P<0.01;95.00%vs 60.00%,P<0.01;95.00% vs 60.00%,P<0.01),而E-cadherin在以上组织中的表达正好相反(24.32% vs 69.23%,P<0.01;10.00% vs 53.33%,P<0.01;10.00% vs 53.33%,P<0.01),其均与患者性别及年龄大小均无关(P>0.05).FAK在Dukes C+D期、伴有淋巴结转移大肠癌组织中的阳性表达显著高于Dukes A+B、无淋巴转移组织(100.00% vs 63.33%,P<0.01;100.00% vs 63-33%,P<0.01),而与肿瘤的分化程度、患者性别及年龄大小均无关(P>0.05).经Spearman相关分析,Ezrin与FAK在大肠癌组织中的表达呈正相关(r=0.346,P<0.05),E-cadherin与Ezrin、FAK在大肠癌组织中的表达均呈明显负相关(r=-0.410,P<0.01;r=-0.406,P<0.01).结论:Ezrin,FAK及E-cadherin在大肠癌组织中的异常表达与肿瘤组织的浸润和转移密切相关,联合检测可以作为一组有效的大肠癌肿瘤标记和预后指标.展开更多
文摘BACKGROUND:p130Cas(p130Crk-associated substance) is a junction protein that is important to the adhesion between cytoskeleton and extracellular matrix.Also the adhesion molecules E-cadherin andβ-catenin play important roles in the invasiveness of carcinoma.This study was undertaken to investigate the effects of p130Cas E-cadherin andβ-catenin on the invasion,metastasis and prognosis of hepatocellular carcinoma(HCC). METHODS:Immunohistochemistry was used to evaluate the expression of p130Cas,E-cadherin,andβ-catenin in 40 patients with HCC.All patients were followed up postoperatively,and the relationship between expression and clinicopathological prognostic parameters was analyzed RESULTS:The positive expression rates of p130Cas and E-cadherin in HCC tissue(n=40)were 62.50%and 55.00% but in normal liver tissue 10%,and 100%,respectively (P<0.05).The abnormal expression rate ofβ-catenin in HCC tissue was 70%,while in normal liver tissue it was 13.33%(P<0.05).The positive rate of p130Cas was correlated with lymph node invasion,pathological stage TNM stage,and a worse prognosis,but not with gender age,HBV infection,hepatic cirrhosis,alpha-fetoprotein (AFP)level before operation,and tumor diameter Similarly,the expression of E-cadherin andβ-catenin was correlated with lymph node invasion,pathological stage,TNM stage,and worse prognosis,but not with gender,age,HBV infection,hepatic cirrhosis,AFP level before operation,and tumor size.Correlations were found between p130Cas and abnormal E-cadherin/β-catenin expression(P<0.001 and<0.05,respectively).CONCLUSIONS:In HCC,there is a negative correlation between the positive expression of p130Cas and the normal expression of the adhesion molecules E-cadherin/β-catenin, and p130Cas plays important roles in the invasion, metastasis and prognosis of HCC.p130Cas may be involved in alterating the structure and function of E-cadherin/ β-catenin,by regulating tyrosine phosphorylation via the p130Cas-Src signal pathway.
基金Supported by Natural Science Foundation Project of Guangxi(2014GXNSFAA118143)Excellent Talents Funding Program of Colleges and Universities in Guangxi(RC20077029)Key Scientific Research Program for Medical Care and Health of Guangxi Health Department(200887)
文摘[Objectives] To observe the effects of oxymatrine on the proliferation,invasion and migration of hepatocellular carcinoma cells,and to explore its possible molecular mechanism.[Methods]The hepatocellular carcinoma cell line Hep G2 was randomly divided into the negative control group and the low,medium and high concentration groups of oxymatrine.The negative control group was added to the cell culture medium,and the low,medium and high concentration groups of oxymatrine were added to the oxymatrine and cell culture medium with the final concentration of 1.0,2.0 and 4.0 mg/m L.The proliferation of each group was measured by MTT assay,and the inhibition rate of cell proliferation was calculated.The invasion and migration ability of each group was determined using Transwell chamber assay.The expression of E-cadherin and CD44 mRNA in each group was detected using Real-time PCR,while the expression of E-cadherin and CD44 protein was detected using Western blotting method.[Results] The inhibition rate of cell proliferation in the high concentration group of oxymatrine was higher than that in the medium and low concentration groups,and the inhibition rate of cell proliferation of medium concentration group was higher than that in the low concentration group.In the cell invasion and cell migration experiments,the number of transmembrane cells of low,medium and high concentration groups of oxymatrine was less than that in the negative control group(P < 0.05),the number of transmembrane cells of high concentration groups of oxymatrine was less than that in medium and low concentration groups,and the number of transmembrane cells of medium concentration group was less than in that of the low concentration group(P < 0.05).Compared with the negative control group,the expression of E-cadherin mRNA and protein increased in the low,medium and high concentration groups of oxymatrine,while the expression of CD44 mRNA and protein decreased(P < 0.05).[Conclusions] Oxymatrine has the effect of inhibiting the proliferation,invasion and migration of hepatocellular carcinoma cell line Hep G2 in vitro.The higher the concentration,the more significant the effect will be.The mechanism is possibly connected with the up-regulation of E-cadherin expression and down-regulation of CD44 expression.
文摘目的:探讨大肠癌组织中埃兹蛋白(Ezrin),黏着斑激酶(FAK)及上皮细胞钙黏蛋白(E-cadherin)的表达及其与肿瘤侵袭和转移的关系.方法:大肠癌组织标本50例,其中高分化腺癌13例,中低分化腺癌37例;无淋巴结转移30例,淋巴结转移20例.采用免疫组织化学方法检测50例大肠癌组织中Ezrin,FAK及E-cadherin的蛋白表达,并运用统计学方法分析Ezrin,FAK及E-cadherin的表达与大肠癌各种临床病理特征的关系及三者的相关性.结果:Ezrin在中低分化、伴有淋巴结转移及Dukes C+D期大肠癌的阳性表达率显著高于高分化、无淋巴结转移及Dukes A+B期大肠癌(83.78% vs 46.15%,P<0.01;95.00%vs 60.00%,P<0.01;95.00% vs 60.00%,P<0.01),而E-cadherin在以上组织中的表达正好相反(24.32% vs 69.23%,P<0.01;10.00% vs 53.33%,P<0.01;10.00% vs 53.33%,P<0.01),其均与患者性别及年龄大小均无关(P>0.05).FAK在Dukes C+D期、伴有淋巴结转移大肠癌组织中的阳性表达显著高于Dukes A+B、无淋巴转移组织(100.00% vs 63.33%,P<0.01;100.00% vs 63-33%,P<0.01),而与肿瘤的分化程度、患者性别及年龄大小均无关(P>0.05).经Spearman相关分析,Ezrin与FAK在大肠癌组织中的表达呈正相关(r=0.346,P<0.05),E-cadherin与Ezrin、FAK在大肠癌组织中的表达均呈明显负相关(r=-0.410,P<0.01;r=-0.406,P<0.01).结论:Ezrin,FAK及E-cadherin在大肠癌组织中的异常表达与肿瘤组织的浸润和转移密切相关,联合检测可以作为一组有效的大肠癌肿瘤标记和预后指标.
