BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its ro...BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its role in hepatocellular carcinoma(HCC)has not been fully deciphered.AIM To decipher the role of CDKN2B-AS1 in the progression of HCC.METHODS CDKN2B-AS1 expression in HCC was detected by quantitative real-time polymerase chain reaction.The malignant phenotypes of Li-7 and SNU-182 cells were detected by the CCK-8 method,EdU method,and flow cytometry,respectively.RNA immunoprecipitation was executed to confirm the interaction between CDKN2B-AS1 and E2F transcription factor 1(E2F1).Luciferase reporter assay and chromatin immunoprecipitation were performed to verify the binding of E2F1 to the promoter of G protein subunit alpha Z(GNAZ).E2F1 and GNAZ were detected by western blot in HCC cells.RESULTS In HCC tissues,CDKN2B-AS1 was upregulated.Depletion of CDKN2B-AS1 inhibited the proliferation of HCC cells,and the depletion of CDKN2B-AS1 also induced cell cycle arrest and apoptosis.CDKN2B-AS1 could interact with E2F1.Depletion of CDKN2B-AS1 inhibited the binding of E2F1 to the GNAZ promoter region.Overexpression of E2F1 reversed the biological effects of depletion of CDKN2B-AS1 on the malignant behaviors of HCC cells.CONCLUSION CDKN2B-AS1 recruits E2F1 to facilitate GNAZ transcription to promote HCC progression.展开更多
采用高效液相色谱/串联质谱技术建立了快速鉴定Z型盐酸头孢吡肟原料药中E型异构体杂质的新方法。以乙腈-醋酸盐缓冲溶液(4∶96)为流动相经C18柱分离,通过高分辨电喷雾串联质谱在线检测,获得了相关的色谱和质谱信息。在所建立条件下,Z型...采用高效液相色谱/串联质谱技术建立了快速鉴定Z型盐酸头孢吡肟原料药中E型异构体杂质的新方法。以乙腈-醋酸盐缓冲溶液(4∶96)为流动相经C18柱分离,通过高分辨电喷雾串联质谱在线检测,获得了相关的色谱和质谱信息。在所建立条件下,Z型盐酸头孢吡肟及其E型异构体杂质获得了有效分离,保留时间分别为5.22 m in和14.60 m in,同时它们的二级质谱及裂解方式呈现明显差异。本法能在无对照品情况下,快速、准确地分离鉴定盐酸头孢吡肟原料药中的Z/E异构体。展开更多
文摘BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its role in hepatocellular carcinoma(HCC)has not been fully deciphered.AIM To decipher the role of CDKN2B-AS1 in the progression of HCC.METHODS CDKN2B-AS1 expression in HCC was detected by quantitative real-time polymerase chain reaction.The malignant phenotypes of Li-7 and SNU-182 cells were detected by the CCK-8 method,EdU method,and flow cytometry,respectively.RNA immunoprecipitation was executed to confirm the interaction between CDKN2B-AS1 and E2F transcription factor 1(E2F1).Luciferase reporter assay and chromatin immunoprecipitation were performed to verify the binding of E2F1 to the promoter of G protein subunit alpha Z(GNAZ).E2F1 and GNAZ were detected by western blot in HCC cells.RESULTS In HCC tissues,CDKN2B-AS1 was upregulated.Depletion of CDKN2B-AS1 inhibited the proliferation of HCC cells,and the depletion of CDKN2B-AS1 also induced cell cycle arrest and apoptosis.CDKN2B-AS1 could interact with E2F1.Depletion of CDKN2B-AS1 inhibited the binding of E2F1 to the GNAZ promoter region.Overexpression of E2F1 reversed the biological effects of depletion of CDKN2B-AS1 on the malignant behaviors of HCC cells.CONCLUSION CDKN2B-AS1 recruits E2F1 to facilitate GNAZ transcription to promote HCC progression.
文摘采用高效液相色谱/串联质谱技术建立了快速鉴定Z型盐酸头孢吡肟原料药中E型异构体杂质的新方法。以乙腈-醋酸盐缓冲溶液(4∶96)为流动相经C18柱分离,通过高分辨电喷雾串联质谱在线检测,获得了相关的色谱和质谱信息。在所建立条件下,Z型盐酸头孢吡肟及其E型异构体杂质获得了有效分离,保留时间分别为5.22 m in和14.60 m in,同时它们的二级质谱及裂解方式呈现明显差异。本法能在无对照品情况下,快速、准确地分离鉴定盐酸头孢吡肟原料药中的Z/E异构体。
